FastQCFastQC Report
Tue 24 May 2016
ERR841086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101308
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC570872.7167364327361816No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA337361.6054762081522556No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT175910.8371452447713519No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT118600.5644103577390844No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA113910.5420909262230953No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG94040.44753077606900077No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG74460.3543507186952127No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC64330.30614265019692494No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC61970.29491155032960426No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT61400.29219895417520897No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT60090.2859647419607216No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57350.27292524465713736No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC53490.2545557338571975No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT51660.2458468725194022No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48740.23195076590390365No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT48700.23176040827903385No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA48420.2304279049049449No Hit
CTCTTTCCCATCTTGCAAGATGGCGGG47960.22823879221894172No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG45490.21648420888322895No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC40280.1916901282439319No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT35680.16979900138389992No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT35620.16951346494659517No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35370.16832372979115864No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA34830.16575390185541578No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT34810.16565872304298085No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC34220.16285094807615066No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT32750.15585530536218395No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT31970.152143331677222No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA31680.15076323889691565No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT31240.14866930502334738No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC30340.1443862584637759No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA30280.14410072202647112No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGGGC29110.1385327614990282No Hit
AGACGGAAGTGAGCGACACACTCTGCGTCCTCGCCTCACCAGGAAACTA28770.13691472168763458No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG28750.13681954287519962No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT28520.13572498653219803No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA28500.13562980771976313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA27350.13015702600475515No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA26950.1282534497560567No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG26690.12701612519440272No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26050.12397040319648524No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA25690.12225718457265665No Hit
CTCTTGAGCTGCTTGCTTGGCCCACTCTCACTCTGTGGAGTGTACATTTG25690.12225718457265665No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT25560.12163852229182966No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC24870.11835485326282488No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT23800.11326278679755658No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA23560.11212064104833752No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT23120.11002670717476924No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTT22480.10698098517685176No Hit
CCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAA22400.10660026992711208No Hit
CTTTTGCTCTGTCAGGCTGGTGGCGTTTTGGTGTCTTCGTTTGTTATG22270.10598160764628507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATAC400.0253.6498644
GCGAACC151.3220666E-5253.6498444
TAATGAG301.2732926E-11253.6498444
CTAGGAC950.0253.6498344
AATCGCA1450.0244.903344
TCAAATG850.0238.7292644
ATATGCC1400.0235.53244
TGTAACA550.0230.5907644
AAACGTA1100.0219.0612244
AGATACT700.0217.4141544
ATCGGCA2700.0216.072144
AACTGGG600.0211.3748844
GACGTTC1150.0209.5368344
GTCTTAC1150.0209.5368344
GATGCCA800.0206.0905244
TTGTCCC253.9914266E-7202.9198844
CTCGTCC451.8189894E-12197.283244
CATTTAG650.0195.1152644
CCAATTC409.822543E-11190.237444
TAAGGTG800.0190.237444
Produced by FastQC (version 0.11.5)