FastQCFastQC Report
Tue 24 May 2016
ERR841085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5512743
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA187490.340102921540148No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA177870.32265244362017237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA172590.3130746345331172No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA156590.2840509706329499No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA140820.2554445219013475No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA138080.25047421945844384No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG115810.21007690726739847No Hit
AAAAAAAAAAAAAAAAAAAAAAAA110900.20117027040803462No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90680.1644916151541982No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT79260.14377597504545378No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC78230.14190757668188053No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC67190.12188124859076506No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC63780.1156955802220419No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC60680.1100722453413845No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC58900.1068433627324909No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA56970.1033423832745332No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGC56860.10314284558521955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCG201.1711745E-7259.547644
TAATGTC500.0259.547644
CGAATAT400.0227.1041744
CATAGTC1700.0190.8438444
TTCGGCG1150.0180.5548744
GGCATCG1250.0176.4923744
CGTACTA451.891749E-10173.0317544
GCCAGCG5100.0160.3088244
ACGAGCC2200.0147.4702544
AGTCCCG453.8366124E-8144.1931344
CTATCAT27450.0143.247644
ATGTTAC3850.0131.4591844
GCGACCA1300.0129.773844
GCATTAG2200.0117.976244
ATCACTG2450.0116.5315944
CGAGTAC251.7541606E-7111.77331543
ATATCGC750.0111.77331543
GCGTACT303.981768E-9111.77331543
ACGAATA359.094947E-11111.77331543
CTACGGG1450.0107.3990244
Produced by FastQC (version 0.11.5)