##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841085.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5512743
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.41672521283869	39.0	37.0	39.0	35.0	39.0
2	37.90319628540637	39.0	38.0	39.0	35.0	39.0
3	38.10006923957819	39.0	38.0	39.0	35.0	39.0
4	38.72706599963031	39.0	38.0	41.0	35.0	41.0
5	39.31018931954564	40.0	39.0	41.0	35.0	41.0
6	39.33817593165507	40.0	39.0	41.0	36.0	41.0
7	39.30085403945005	40.0	39.0	41.0	35.0	41.0
8	39.23860245253588	40.0	39.0	41.0	35.0	41.0
9	39.1895807586169	40.0	39.0	41.0	35.0	41.0
10	39.213926352090056	40.0	39.0	41.0	35.0	41.0
11	39.13738460145884	40.0	39.0	41.0	35.0	41.0
12	39.01784338577002	40.0	38.0	41.0	35.0	41.0
13	38.969987536150335	40.0	38.0	41.0	35.0	41.0
14	38.9629902935798	40.0	38.0	41.0	35.0	41.0
15	38.91024159842024	40.0	38.0	41.0	35.0	41.0
16	38.94030993282291	40.0	38.0	41.0	35.0	41.0
17	38.95076280537656	40.0	38.0	41.0	35.0	41.0
18	38.95157691914896	40.0	38.0	41.0	35.0	41.0
19	38.927424695836535	40.0	38.0	41.0	35.0	41.0
20	38.97754693081103	40.0	38.0	41.0	35.0	41.0
21	39.00244814604998	40.0	38.0	41.0	35.0	41.0
22	39.00054691466662	40.0	38.0	41.0	35.0	41.0
23	38.93968356587637	40.0	38.0	41.0	35.0	41.0
24	38.86651436499035	40.0	38.0	41.0	35.0	41.0
25	38.87306601626669	40.0	38.0	41.0	35.0	41.0
26	38.80779918813248	40.0	38.0	41.0	35.0	41.0
27	38.75393186082053	40.0	38.0	41.0	35.0	41.0
28	38.728416672454884	40.0	38.0	41.0	35.0	41.0
29	38.71753586380233	40.0	38.0	41.0	35.0	41.0
30	38.749118537174624	40.0	38.0	41.0	35.0	41.0
31	38.66133563901707	40.0	38.0	41.0	35.0	41.0
32	38.52741763729479	40.0	38.0	41.0	35.0	41.0
33	38.41469980751487	40.0	37.0	41.0	34.0	41.0
34	38.437106322105386	40.0	37.0	41.0	34.0	41.0
35	38.41190914460969	40.0	37.0	41.0	34.0	41.0
36	38.379353494447706	40.0	37.0	41.0	34.0	41.0
37	38.348765709179034	40.0	37.0	41.0	34.0	41.0
38	38.25080733863506	40.0	36.0	41.0	34.0	41.0
39	38.21340321554204	40.0	36.0	41.0	34.0	41.0
40	38.10407105170261	40.0	36.0	41.0	34.0	41.0
41	38.06058795220624	39.0	36.0	41.0	34.0	41.0
42	37.95637699885689	39.0	35.0	41.0	34.0	41.0
43	37.82567198477396	39.0	35.0	41.0	34.0	41.0
44	37.648923542087694	39.0	35.0	41.0	34.0	41.0
45	37.4145102448627	39.0	35.0	41.0	33.0	41.0
46	37.19299763965382	39.0	35.0	41.0	33.0	41.0
47	36.90276960769792	38.0	35.0	40.0	32.0	41.0
48	36.94644008691659	38.0	35.0	40.0	32.0	41.0
49	36.710067926558615	38.0	35.0	40.0	32.0	41.0
50	36.65977034498286	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	7.0
20	19.0
21	96.0
22	176.0
23	325.0
24	549.0
25	989.0
26	1795.0
27	3527.0
28	6887.0
29	13504.0
30	25054.0
31	46430.0
32	81202.0
33	146758.0
34	256405.0
35	218769.0
36	339663.0
37	572387.0
38	785044.0
39	1210731.0
40	1802425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.12674924261842	20.107920866254787	31.765329891126793
2	24.607060405319096	23.268434606873566	29.932249698562043	22.1922552892453
3	20.783410363951305	22.657722299044234	30.770888466957373	25.78797887004709
4	20.847788478439863	22.48374357375267	33.75183642698381	22.916631520823664
5	17.73008464207383	25.109260489741676	33.06863751856381	24.092017349620686
6	21.304058614740427	26.817811024384774	29.490291856522244	22.38783850435255
7	25.586645341529618	26.126685027762043	25.172024162925787	23.114645467782555
8	24.07126542993207	25.319809031547454	26.433954929515124	24.174970609005353
9	23.300723432962503	26.681182126574736	26.917380331352287	23.100714109110474
10	24.395024400738436	22.587938527154268	27.65146135054727	25.36557572156003
11	26.17473733130676	23.675999407191664	26.57551785018819	23.57374541131339
12	23.10220157188536	26.893925582962964	26.5615320721463	23.44234077300538
13	25.18880346861807	25.768533015233974	25.934113017784433	23.10855049836352
14	21.986296114293737	27.97830408564303	24.18560052590879	25.849799274154446
15	22.297103275084655	30.13245856010338	25.978682481661124	21.591755683150836
16	24.619014526887977	30.23465813661185	23.77279332629872	21.373534010201457
17	23.852608402024185	31.674884898497897	22.277040667413665	22.195466032064257
18	23.431021544084317	29.179321437621887	21.716503018551744	25.673153999742055
19	23.345528714108386	27.359084216332956	24.026115492777368	25.26927157678129
20	25.88845516651148	25.03653807188182	25.751136956683812	23.323869804922882
21	24.853997365739705	28.183301851727897	23.111017509795033	23.851683272737365
22	24.347969785640288	28.78325726412423	24.147379988510256	22.721392961725225
23	26.279222521347357	26.882533794882146	25.32936870084457	21.508874982925924
24	25.330166851601827	28.610874840347172	24.304670107059227	21.754288200991777
25	25.5412728222987	27.285458762750253	25.604847352718245	21.568421062232808
26	25.59033766350161	29.562108343157373	24.870555008772303	19.976998984568713
27	26.502492219907413	28.021673247212675	25.387376452869848	20.088458080010067
28	24.049163994751023	27.918008225989915	26.02908896452159	22.003738814737474
29	25.214655266618685	27.84084271928073	23.43527558699068	23.50922642710991
30	27.220799785925028	28.76892826435827	23.872343353146924	20.137928596569783
31	24.4796720212361	28.207093556850715	24.992985574338096	22.320248847575087
32	23.833554100564395	27.812421049151993	26.550348702038225	21.803676148245394
33	24.435938061085338	24.568761011417127	29.052276605796823	21.94302432170071
34	23.156869276574497	27.648604264672567	26.129929561396615	23.064596897356328
35	22.787973480968937	25.000186676994108	27.761835674599194	24.45000416743776
36	23.46454120745397	25.224744534333706	27.607131841885874	23.70358241632646
37	21.811847115475636	27.093775159878142	26.049953840688794	25.04442388395742
38	22.793122683766065	26.303641968149115	26.217625398555374	24.685609949529447
39	21.872995878525792	28.432179554508103	25.861670905527884	23.833153661438224
40	18.692575638644936	27.31667016652382	27.93557817399779	26.05517602083346
41	20.84817875568573	28.377547905012758	25.59760306757673	25.176670271724788
42	22.198530833292924	29.64491981681095	24.912778761990122	23.243770587906003
43	22.54718119641072	26.164895128220383	27.498200246559932	23.789723428808962
44	23.597942344436856	27.847241034746045	24.536152053522105	24.018664567294994
45	21.575279155459576	26.69067060988833	28.19189757847498	23.542152656177116
46	21.641075887817586	26.58531698520752	26.044514272031122	25.729092854943776
47	22.35393096280171	28.110698539711663	28.092670288680544	21.44270020880608
48	22.959446630559313	29.092444381526704	24.96721420515033	22.980894782763652
49	18.838261273133973	28.747447812678562	25.597546819834697	26.816744094352767
50	19.57904919153762	28.46659590461657	27.155591929314195	24.79876297453162
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	157656.0
1	155401.0
2	110074.5
3	65321.5
4	60775.0
5	42844.0
6	18594.5
7	9689.0
8	9644.5
9	9115.0
10	9421.0
11	9873.5
12	9360.5
13	8712.0
14	9385.0
15	9981.0
16	9524.5
17	9657.0
18	11187.0
19	14788.0
20	19095.5
21	24184.0
22	29993.0
23	34161.5
24	36781.0
25	42333.0
26	53423.5
27	63438.5
28	65458.5
29	68303.0
30	80513.0
31	101324.5
32	131271.5
33	151924.5
34	165946.0
35	182259.0
36	190250.5
37	201123.5
38	216649.0
39	223070.0
40	233586.5
41	264457.0
42	305196.0
43	344627.0
44	361999.5
45	368556.5
46	418445.0
47	458766.5
48	481854.5
49	521815.5
50	552312.5
51	558379.0
52	534298.5
53	508891.5
54	475954.0
55	433738.0
56	390947.5
57	351741.0
58	335745.0
59	321242.0
60	267329.0
61	199266.5
62	159020.0
63	127439.5
64	98604.5
65	85711.0
66	61138.0
67	33794.5
68	22675.5
69	16276.5
70	12941.0
71	9939.0
72	6407.0
73	3537.0
74	1717.0
75	1146.5
76	800.5
77	562.0
78	360.0
79	221.5
80	146.5
81	66.5
82	37.0
83	22.5
84	17.5
85	12.0
86	6.0
87	4.5
88	4.5
89	4.0
90	3.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	30988.0
25	36778.0
26	40157.0
27	49112.0
28	48128.0
29	45872.0
30	65274.0
31	114158.0
32	89163.0
33	18710.0
34	19320.0
35	20369.0
36	22379.0
37	23742.0
38	15822.0
39	20192.0
40	21404.0
41	25866.0
42	32433.0
43	55274.0
44	179181.0
45	445801.0
46	548183.0
47	733006.0
48	863592.0
49	1108406.0
50	839433.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.96272414745772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.15260751050252	7.909891326574253
2	11.360283613518538	2.4907931098377585
3	4.48038542782757	1.4735168855868896
4	2.3758950648356767	1.0418512879639878
5	1.4526278530219203	0.7962379220796536
6	0.9873348158985085	0.649432753672578
7	0.6826137397310651	0.5238314289555316
8	0.5280002907734694	0.4630657230021609
9	0.41420726077057046	0.4086755945731664
>10	3.462171885918853	7.7745416833335135
>50	0.5380472419762704	4.210608921874522
>100	1.2565386447618925	36.43191506235644
>500	0.24355091178872765	16.95845391408925
>1k	0.05981952219372258	12.147190558488896
>5k	0.0032867869337210214	2.3955967272318675
>10k+	0.0026294295469768167	4.324397100379522
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	18749	0.340102921540148	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	17787	0.32265244362017237	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	17259	0.3130746345331172	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	15659	0.2840509706329499	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	14082	0.2554445219013475	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13808	0.25047421945844384	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	11581	0.21007690726739847	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	11090	0.20117027040803462	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9068	0.1644916151541982	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	7926	0.14377597504545378	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	7823	0.14190757668188053	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	6719	0.12188124859076506	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	6378	0.1156955802220419	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	6068	0.1100722453413845	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	5890	0.1068433627324909	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	5697	0.1033423832745332	No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGC	5686	0.10314284558521955	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.08527515249667905	0.0	0.0	0.0	0.0
2	0.14009359768812005	0.0	0.0	0.0	0.0
3	0.1895608048479677	0.0	0.0	0.0	0.0
4	0.2627729970361397	0.0	0.0	0.0	0.0
5	0.3062177939367027	0.0	0.0	0.0	0.0
6	0.40859876834454284	0.0	0.0	0.0	0.0
7	0.45547198554331303	0.0	0.0	0.0	0.0
8	0.5106895061133813	0.0	0.0	0.0	0.0
9	0.5718024584131711	0.0	0.0	0.0	0.0
10	0.6157188898521118	0.0	0.0	0.0	0.0
11	0.6696484853366101	0.0	0.0	0.0	0.0
12	0.7169751972838204	0.0	0.0	0.0	0.0
13	0.7650275008285349	0.0	0.0	0.0	0.0
14	0.8057331894485196	0.0	0.0	0.0	0.0
15	0.8552366761882424	0.0	0.0	0.0	0.0
16	0.9007312693517546	0.0	0.0	0.0	0.0
17	0.9595767479093439	0.0	0.0	0.0	0.0
18	0.9944232843794822	0.0	0.0	0.0	0.0
19	1.0329521982069543	0.0	0.0	0.0	0.0
20	1.076270016577954	0.0	0.0	0.0	0.0
21	1.114291016287173	0.0	0.0	0.0	0.0
22	1.147504971662927	0.0	0.0	0.0	0.0
23	1.1805738087191802	0.0	0.0	0.0	0.0
24	1.2155654635088196	0.0	0.0	0.0	0.0
25	1.2494324513223272	0.0	0.0	0.0	0.0
26	1.2854399343484724	0.0	0.0	0.0	0.0
27	1.3147719746775788	0.0	0.0	0.0	0.0
28	1.3472966180357038	0.0	0.0	0.0	0.0
29	1.3825059503045942	0.0	0.0	0.0	0.0
30	1.4133798727783973	0.0	0.0	0.0	0.0
31	1.4430384293263807	0.0	0.0	0.0	0.0
32	1.4733137387322428	0.0	0.0	0.0	0.0
33	1.5015936712449682	0.0	0.0	0.0	0.0
34	1.5313610665325774	0.0	0.0	0.0	0.0
35	1.5576093425722912	0.0	0.0	0.0	0.0
36	1.581336187810678	0.0	0.0	0.0	0.0
37	1.5964466328287026	0.0	0.0	0.0	0.0
38	1.6101058946517188	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCACTCG	20	1.1711745E-7	259.5476	44
TAATGTC	50	0.0	259.5476	44
CGAATAT	40	0.0	227.10417	44
CATAGTC	170	0.0	190.84384	44
TTCGGCG	115	0.0	180.55487	44
GGCATCG	125	0.0	176.49237	44
CGTACTA	45	1.891749E-10	173.03175	44
GCCAGCG	510	0.0	160.30882	44
ACGAGCC	220	0.0	147.47025	44
AGTCCCG	45	3.8366124E-8	144.19313	44
CTATCAT	2745	0.0	143.2476	44
ATGTTAC	385	0.0	131.45918	44
GCGACCA	130	0.0	129.7738	44
GCATTAG	220	0.0	117.9762	44
ATCACTG	245	0.0	116.53159	44
CGAGTAC	25	1.7541606E-7	111.773315	43
ATATCGC	75	0.0	111.773315	43
GCGTACT	30	3.981768E-9	111.773315	43
ACGAATA	35	9.094947E-11	111.773315	43
CTACGGG	145	0.0	107.39902	44
>>END_MODULE