##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841084.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2145066
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.16227519339731	39.0	37.0	39.0	33.0	39.0
2	37.717252988952325	39.0	38.0	39.0	35.0	39.0
3	37.79898707079409	39.0	37.0	39.0	35.0	39.0
4	38.191002514607945	39.0	38.0	40.0	35.0	41.0
5	38.94537976920058	40.0	38.0	41.0	35.0	41.0
6	38.88791580305688	40.0	38.0	41.0	35.0	41.0
7	38.95427693133917	40.0	38.0	41.0	35.0	41.0
8	38.88459515930978	40.0	38.0	41.0	35.0	41.0
9	38.76976932178311	40.0	38.0	41.0	35.0	41.0
10	38.777136927255384	40.0	38.0	41.0	35.0	41.0
11	38.67364827002992	40.0	38.0	41.0	35.0	41.0
12	38.67050244607858	40.0	38.0	41.0	35.0	41.0
13	38.54151620509579	40.0	38.0	41.0	35.0	41.0
14	38.49143942424149	40.0	38.0	41.0	35.0	41.0
15	38.32427813409937	40.0	37.0	41.0	34.0	41.0
16	38.424666187427334	40.0	37.0	41.0	35.0	41.0
17	38.58607101133485	40.0	38.0	41.0	35.0	41.0
18	38.57289752389903	40.0	38.0	41.0	35.0	41.0
19	38.58352470273642	40.0	38.0	41.0	35.0	41.0
20	38.612457145840736	40.0	38.0	41.0	35.0	41.0
21	38.611738753026714	40.0	38.0	41.0	35.0	41.0
22	38.618726882995674	40.0	38.0	41.0	35.0	41.0
23	38.62086201543449	40.0	38.0	41.0	35.0	41.0
24	38.49167111874413	40.0	37.0	41.0	35.0	41.0
25	38.52229865155003	40.0	37.0	41.0	35.0	41.0
26	38.48571423157922	40.0	37.0	41.0	35.0	41.0
27	38.40811452991289	40.0	37.0	41.0	34.0	41.0
28	38.423464044372324	40.0	37.0	41.0	34.0	41.0
29	38.39748022149761	40.0	37.0	41.0	34.0	41.0
30	38.476673599275976	40.0	37.0	41.0	34.0	41.0
31	38.434864002315194	40.0	37.0	41.0	34.0	41.0
32	38.33286321640686	40.0	37.0	41.0	34.0	41.0
33	38.2019664753508	40.0	37.0	41.0	34.0	41.0
34	38.182246340844664	40.0	36.0	41.0	34.0	41.0
35	38.24501081775482	40.0	36.0	41.0	34.0	41.0
36	38.23643385031527	40.0	36.0	41.0	34.0	41.0
37	38.162491501684805	40.0	36.0	41.0	34.0	41.0
38	38.06300652242206	40.0	36.0	41.0	34.0	41.0
39	37.92901419277204	39.0	35.0	41.0	34.0	41.0
40	37.86250758581988	39.0	35.0	41.0	34.0	41.0
41	37.781364131444846	39.0	35.0	41.0	34.0	41.0
42	37.726282517230075	39.0	35.0	41.0	34.0	41.0
43	37.662123686923294	39.0	35.0	41.0	34.0	41.0
44	37.409731562018806	38.0	35.0	40.0	34.0	41.0
45	37.25216091095996	38.0	35.0	40.0	33.0	41.0
46	36.941593389863634	37.0	35.0	40.0	33.0	41.0
47	36.64997987742807	37.0	35.0	40.0	32.0	41.0
48	36.52062241829156	37.0	35.0	40.0	32.0	41.0
49	36.81855235469477	37.0	35.0	40.0	33.0	41.0
50	36.09961609644787	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	13.0
21	39.0
22	79.0
23	155.0
24	302.0
25	610.0
26	1015.0
27	2030.0
28	3743.0
29	7288.0
30	13281.0
31	23227.0
32	39577.0
33	66226.0
34	106597.0
35	111221.0
36	185135.0
37	249950.0
38	304774.0
39	503690.0
40	526112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	54.248820316018254	15.548705727469459	30.202473956512293
2	23.35172903770793	28.17209353931301	32.790040026740435	15.686137396238625
3	24.91354578367286	28.97253511080778	22.659722358193175	23.45419674732619
4	22.262811493912075	31.242721669170088	26.052391861136208	20.442074975781633
5	15.375564201754166	32.94835683377574	25.227242425174794	26.448836539295296
6	16.571098511654185	34.423602816883026	23.694375837386822	25.310922834075967
7	20.879497414065582	31.83030265735413	23.27462185312713	24.015578075453156
8	19.673054348910476	30.13594919690117	28.051631045385083	22.139365408803272
9	17.022693008047305	35.58697028436421	25.5356245448858	21.854712162702686
10	25.17381749559221	27.583673416109338	25.184633013622893	22.05787607467556
11	16.772490916363413	30.370673909334258	26.937259739327367	25.919575434974963
12	20.286275573805188	30.14802341746128	25.66331292370491	23.90238808502862
13	18.81489893551061	31.201510815984218	24.432161994083167	25.55142825442201
14	16.421452766488304	33.04364527711502	23.38445530347318	27.1504466529235
15	17.449253309688373	37.25573012671871	27.26503520171407	18.029981361878843
16	18.114873854697244	40.54616501310449	20.064557454176235	21.27440367802203
17	17.283057957191062	42.948748430118236	19.08458760709461	20.683606005596097
18	20.350189691133046	32.315042987022316	22.864797633266296	24.469969688578345
19	21.406101257490445	30.846090516562196	20.96965781006272	26.77815041588464
20	20.353639468435937	26.92546523044046	26.58510274275943	26.135792558364173
21	16.30304149149723	38.11276669342575	23.92178142770432	21.662410387372695
22	17.84527842033765	33.92506337800329	22.66219314463984	25.567465057019223
23	22.042398695424755	30.02387805316946	28.496885410518836	19.436837840886948
24	17.190566630583863	34.404629041717136	25.2042594493596	23.200544878339407
25	17.57543803144427	31.21581162171286	26.693450057208533	24.51530028963434
26	17.723466478962372	38.70737929878046	27.40684811204658	16.16230611021059
27	19.33675926182984	32.851721691813864	23.150769254334026	24.660749792022273
28	18.584832998280334	27.2516772834064	26.27015767675055	27.89333204156272
29	17.51842497139407	32.30143995063419	28.84126446816549	21.338870609806246
30	20.223714491074123	31.829220377003477	28.695339885783316	19.251725246139088
31	19.639777966244758	33.77443315875574	24.76390826034704	21.821880614652457
32	17.68072130681949	34.35207892651924	24.547408167570715	23.41979159909055
33	16.88733460365814	27.382996651928735	31.453144524640308	24.276524219772817
34	17.607224661921727	36.25172866759843	27.451687909647603	18.68935876083224
35	17.88602206969522	30.431561497855945	26.59607989436223	25.086336538086613
36	15.693029679141274	31.340377992155915	26.747973153737885	26.218619174964918
37	15.430499040174675	36.10710557335565	23.703094740093526	24.759300646376147
38	20.761399037930744	31.32661339141946	24.357131542356274	23.554856028293518
39	19.854143225271848	32.69160563428534	27.418215181566204	20.03603595887661
40	15.957554885321754	30.36005029082784	28.65980534073971	25.022589483110703
41	16.53714661122805	33.70348839722173	25.67047158842877	24.088893403121446
42	15.227977390096942	36.20101654315524	27.491571847115125	21.079434219632688
43	19.656466598089253	26.999617675444792	26.505827436880846	26.838088289585105
44	17.475073932430103	32.22772904934052	21.06275549669138	29.234441521537995
45	14.963303570566278	28.636565311918226	34.51719724380529	21.8829338737102
46	16.882509812955	33.194529618368406	29.06025371856929	20.862706850107305
47	16.158819820123576	34.16324893809494	28.767888822969432	20.910042418812058
48	21.046991364375362	29.94060976859726	25.001145805643034	24.01125306138435
49	18.330962726263078	29.491407361222066	25.923562535315554	26.25406737719931
50	19.530953849813066	31.301332699068674	27.33092263209924	21.83679081901902
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	109487.0
1	107308.5
2	65784.0
3	25928.5
4	24259.0
5	17709.0
6	8051.0
7	3787.0
8	3803.5
9	3553.0
10	2959.5
11	2394.0
12	1980.5
13	2075.5
14	3050.0
15	3852.0
16	3580.0
17	3307.5
18	4619.5
19	8093.5
20	10786.0
21	12695.5
22	15554.5
23	18870.0
24	22811.5
25	27946.5
26	32580.0
27	38495.5
28	44066.0
29	47665.5
30	55367.5
31	72888.5
32	98441.5
33	112357.5
34	122600.0
35	140614.5
36	140579.0
37	131388.5
38	137444.0
39	137298.0
40	127209.5
41	135071.0
42	155588.5
43	169151.5
44	168490.0
45	167063.0
46	173920.0
47	175117.0
48	185555.0
49	195818.0
50	208277.0
51	214150.5
52	170392.5
53	122050.5
54	104465.5
55	91581.5
56	74647.5
57	58893.0
58	46390.0
59	36296.0
60	28730.5
61	22214.5
62	15277.5
63	10440.0
64	7205.5
65	5387.0
66	3668.0
67	2450.0
68	1790.0
69	1298.5
70	977.5
71	797.0
72	636.0
73	473.5
74	379.0
75	359.5
76	371.0
77	360.5
78	505.5
79	817.0
80	898.5
81	829.5
82	633.0
83	226.5
84	11.0
85	7.5
86	3.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	15139.0
25	18805.0
26	21956.0
27	24816.0
28	28933.0
29	22226.0
30	22863.0
31	32659.0
32	45822.0
33	7924.0
34	6117.0
35	6444.0
36	7165.0
37	9280.0
38	6783.0
39	7755.0
40	9443.0
41	11565.0
42	13697.0
43	19633.0
44	45788.0
45	127972.0
46	238324.0
47	193928.0
48	378861.0
49	283012.0
50	538156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.56526973157301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.94261622070698	5.29134757441542
2	11.722286943111273	1.7736452519106642
3	4.766460717408986	1.0817868297643793
4	2.6277228471241387	0.7951772847324441
5	1.577113825552013	0.5965645743846981
6	1.10483715668941	0.5015034659891748
7	0.7776997011676551	0.41184556066479244
8	0.571538394469123	0.34590736928872734
9	0.45779837597460205	0.3117031377231544
>10	3.7887384777647375	5.8644689008341295
>50	0.6406203262431344	3.441531230868289
>100	1.4053364763325555	27.870765144473914
>500	0.48858669912255287	25.84453948598978
>1k	0.11435007851804428	13.37953819266808
>5k	0.007796596262593928	4.198336811822615
>10k+	0.0064971635521616075	8.291339184469734
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	41909	1.9537394187404955	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	23701	1.1049077277808703	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	17484	0.8150798157259496	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	15498	0.7224952518943473	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	13067	0.6091654056332065	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	12543	0.5847372528397727	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	12518	0.5835717875347426	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	11853	0.5525704104209381	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10110	0.4713141693542296	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	10002	0.46627935923649905	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	9834	0.45844743238669583	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	9235	0.4305228836781712	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	8495	0.39602511064927604	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	8253	0.38474340649658334	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	7481	0.3487538378772495	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	7138	0.33276365389223456	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	6892	0.321295475290737	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6679	0.3113657108918793	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	5645	0.2631620658758285	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	5535	0.25803401853369545	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	5129	0.23910686198000433	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	5098	0.23766168500176685	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	4500	0.20978375490544346	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	4402	0.20521513090972493	No Hit
ATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACT	4186	0.19514551067426364	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3926	0.18302467150194912	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	3913	0.1824186295433334	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	3755	0.1750528888155423	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	3747	0.1746799399179326	No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC	3492	0.16279219380662413	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	3414	0.15915594205492978	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	3374	0.1572911975668814	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT	3184	0.14843366124865157	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	3108	0.14489064672135962	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3086	0.143865037252933	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	3061	0.14269957194790278	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAG	2969	0.13841065962539148	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	2878	0.1341683659150814	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	2790	0.13006592804137496	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA	2632	0.12270018731358383	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	2485	0.11584725132000599	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	2366	0.11029963646806205	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG	2358	0.10992668757045239	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2306	0.10750251973598948	No Hit
ATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATA	2267	0.10568439386014229	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA	2243	0.10456554716731326	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0820953760863302	0.0	0.0	0.0	0.0
2	0.1538414202639919	0.0	0.0	0.0	0.0
3	0.223023440770587	0.0	0.0	0.0	0.0
4	0.29430329882623657	0.0	0.0	0.0	0.0
5	0.34875383787724945	0.0	0.0	0.0	0.0
6	0.44464832317513775	0.0	0.0	0.0	0.0
7	0.5128513528255074	0.0	0.0	0.0	0.0
8	0.5930819844237893	0.0	0.0	0.0	0.0
9	0.6648746472136522	0.0	0.0	0.0	0.0
10	0.7247795638922065	0.0	0.0	0.0	0.0
11	0.7929825935425763	0.0	0.0	0.0	0.0
12	0.8544725430359719	0.0	0.0	0.0	0.0
13	0.908550133189375	0.0	0.0	0.0	0.0
14	0.9531641450659327	0.0	0.0	0.0	0.0
15	1.0177775415768093	0.0	0.0	0.0	0.0
16	1.0751184345842972	0.0	0.0	0.0	0.0
17	1.135536155997065	0.0	0.0	0.0	0.0
18	1.1843924615839325	0.0	0.0	0.0	0.0
19	1.2368384003102935	0.0	0.0	0.0	0.0
20	1.2852285197751492	0.0	0.0	0.0	0.0
21	1.333245690342395	0.0	0.0	0.0	0.0
22	1.3715195709595882	0.0	0.0	0.0	0.0
23	1.4155741594897313	0.0	0.0	0.0	0.0
24	1.4622860089153433	0.0	0.0	0.0	0.0
25	1.5074128255261143	0.0	0.0	0.0	0.0
26	1.553611870217513	0.0	0.0	0.0	0.0
27	1.5888555410416276	0.0	0.0	0.0	0.0
28	1.6296002081054848	0.0	0.0	0.0	0.0
29	1.6726291871672014	0.0	0.0	0.0	0.0
30	1.709877458315968	0.0	0.0	0.0	0.0
31	1.745447459425491	0.0	0.0	0.0	0.0
32	1.7802249441275932	0.0	0.0	0.0	0.0
33	1.8120188376488182	0.0	0.0	0.0	0.0
34	1.8505258113270175	0.0	0.0	0.0	0.0
35	1.8827858909702546	0.0	0.0	0.0	0.0
36	1.908892313802932	0.0	0.0	0.0	0.0
37	1.926047963092977	0.0	0.0	0.0	0.0
38	1.942038147077992	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTT	10	0.0056438665	156.49503	44
GTATACT	10	0.0056438665	156.49503	44
GTCGAAC	100	0.0	156.49501	44
GTCAATG	75	0.0	156.49501	44
CAGGTCA	85	0.0	147.28943	44
AATGACG	85	0.0	147.28943	44
ACAGTAT	75	0.0	146.06201	44
ACTACGG	130	0.0	144.45694	44
TTGCAGA	130	0.0	144.45694	44
ACTTGTA	55	0.0	142.2682	44
GGTAAAC	105	0.0	141.59073	44
TAACGCC	105	0.0	141.59073	44
TACTAAG	100	0.0	140.8455	44
AATCTTT	90	0.0	139.10669	44
AATCGCA	45	0.0	139.10669	44
AAACGGA	130	0.0	138.43791	44
ACATTGG	655	0.0	137.38112	44
CCCGTAC	40	1.8189894E-11	136.93314	44
TCCCGAG	155	0.0	136.30211	44
TTACTAG	70	0.0	134.13858	44
>>END_MODULE