FastQCFastQC Report
Tue 24 May 2016
ERR841083.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841083.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3435129
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC253870.7390406590261966No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA169650.49386791587739504No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA165240.4810299700535264No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA155680.4531998652743463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA146030.4251077616008016No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA144170.4196931177839318No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT138620.40353651929811074No Hit
AAAAAAAAAAAAAAAAAAAAAAAA116570.3393467901787677No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA114550.3334663705496941No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG98290.28613190363447777No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT77410.2253481601418753No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA76070.22144728771466807No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC72200.21018133525698746No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71520.20820178805512107No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC65820.19160852474535892No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA56850.16549596827368057No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC52740.15353135209769414No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA48590.14145029196865677No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG48190.14028585243814715No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA47980.1396745216846296No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45250.1317272218889014No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA44330.12904901096872928No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA43830.12759346155559223No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42640.12412925395232612No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA42360.12331414628096937No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG42310.12316859133965567No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT41290.12019927053685611No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG39960.1163275090979116No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39920.11621106514486065No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA36550.10640066210031705No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT35040.10200490287264322No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT34780.10124801717781196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAC950.0186.9542444
GGGCACC154.4668326E-5186.9542244
GACGTAT1250.0179.4760644
GATTGAC850.0175.9569444
ACGAACA2950.0174.2793744
CCCGGGC1200.0171.3747144
GACCAAT1150.0170.6973444
GTTCATG550.0169.958444
GTACAGG1000.0158.911144
CTACGAC600.0155.795244
GATCCCA1050.0151.3439244
AATGTAC1000.0140.2156844
TCGTAGG600.0140.2156744
TAGGCAT2400.0140.2156744
TCTCGCC1300.0136.620444
GCTATAC700.0133.5387444
CATGACC1900.0132.835944
CGCTACG1050.0124.6361644
CTTCGGG451.8644641E-9124.63615444
GTGGGTT304.5228353E-6124.63615444
Produced by FastQC (version 0.11.5)