##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841083.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3435129
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.709373650887635	39.0	37.0	39.0	32.0	39.0
2	37.3778350099807	39.0	37.0	39.0	34.0	39.0
3	37.46336454904605	39.0	37.0	39.0	35.0	39.0
4	37.982494689427966	39.0	37.0	40.0	35.0	41.0
5	38.77829886446768	40.0	38.0	41.0	35.0	41.0
6	38.75863118968749	40.0	38.0	41.0	35.0	41.0
7	38.75625340416619	40.0	38.0	41.0	35.0	41.0
8	38.718642298440614	40.0	38.0	41.0	35.0	41.0
9	38.69957722111746	40.0	38.0	41.0	35.0	41.0
10	38.680210262846025	40.0	38.0	41.0	35.0	41.0
11	38.63939927729061	40.0	38.0	41.0	35.0	41.0
12	38.45442543788021	40.0	37.0	41.0	34.0	41.0
13	38.32503000615115	40.0	37.0	41.0	34.0	41.0
14	38.40427157175174	40.0	37.0	41.0	34.0	41.0
15	38.22043917419113	40.0	37.0	41.0	34.0	41.0
16	38.16216829120537	40.0	36.0	41.0	34.0	41.0
17	38.28411305659846	40.0	35.0	41.0	35.0	41.0
18	38.266738454363725	40.0	35.0	41.0	35.0	41.0
19	38.232846568498594	40.0	35.0	41.0	35.0	41.0
20	38.240480925170495	40.0	35.0	41.0	34.0	41.0
21	38.23079016828771	40.0	35.0	41.0	34.0	41.0
22	38.277884760659646	40.0	35.0	41.0	35.0	41.0
23	38.24270034691565	40.0	35.0	41.0	34.0	41.0
24	38.17433173543119	40.0	35.0	41.0	34.0	41.0
25	38.11088896465775	40.0	35.0	41.0	34.0	41.0
26	38.082846018684684	40.0	35.0	41.0	34.0	41.0
27	38.07721198166374	40.0	35.0	41.0	34.0	41.0
28	38.06465001403551	40.0	35.0	41.0	34.0	41.0
29	38.085880881297236	40.0	35.0	41.0	34.0	41.0
30	38.03969745240793	40.0	35.0	41.0	34.0	41.0
31	38.10903607913383	40.0	35.0	41.0	34.0	41.0
32	37.932956163794366	40.0	35.0	41.0	34.0	41.0
33	37.872489783812284	40.0	35.0	41.0	34.0	41.0
34	37.875799538771275	40.0	35.0	41.0	34.0	41.0
35	37.925457380619925	40.0	35.0	41.0	34.0	41.0
36	37.941817103002755	39.0	35.0	41.0	34.0	41.0
37	37.89402741024633	39.0	35.0	41.0	34.0	41.0
38	37.81234811926008	39.0	35.0	41.0	34.0	41.0
39	37.81958717857733	39.0	35.0	41.0	34.0	41.0
40	37.75071035762598	39.0	35.0	41.0	34.0	41.0
41	37.72350796747213	39.0	35.0	41.0	34.0	41.0
42	37.585957760673075	39.0	35.0	41.0	34.0	41.0
43	37.446716762003874	39.0	35.0	41.0	34.0	41.0
44	37.35218354186507	38.0	35.0	41.0	34.0	41.0
45	37.15531960164987	38.0	35.0	40.0	33.0	41.0
46	36.89843078266929	38.0	35.0	40.0	33.0	41.0
47	36.713776158592204	37.0	35.0	40.0	32.0	41.0
48	36.72851587643448	37.0	35.0	40.0	32.0	41.0
49	36.833269407521925	37.0	35.0	40.0	32.0	41.0
50	36.84152002260381	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	21.0
22	54.0
23	138.0
24	401.0
25	879.0
26	1806.0
27	3702.0
28	7277.0
29	13397.0
30	23844.0
31	41379.0
32	70271.0
33	133870.0
34	330300.0
35	178197.0
36	240468.0
37	368991.0
38	431531.0
39	664714.0
40	923883.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.409963934396636	19.0013824808326	31.58865358477076
2	24.42892246550275	24.239701041794937	30.730810982644318	20.60056551005799
3	24.09740653116666	25.166594908080597	27.946636065195808	22.789362495556936
4	17.76725124442197	25.88784875327826	31.93641345055746	24.408486551742307
5	15.456682995019982	27.71823125128634	30.67931364440753	26.145772109286142
6	18.501168369513927	30.263783397945172	32.75041490436021	18.48463332818069
7	20.429160011166974	31.111524487144443	25.330984658800293	23.128330842888285
8	23.84801269471976	26.730146087672395	28.305225218616243	21.116615998991595
9	21.706375510206456	29.016086441004106	27.95018760576386	21.327350443025576
10	24.75118110557129	25.085200584897976	26.851218687857138	23.312399621673595
11	23.694423120645542	27.468546304956813	26.97159262432357	21.865437950074075
12	23.005890026255198	27.97350550736231	27.940231647778003	21.080372818604484
13	20.77546432754054	26.58892868360984	27.43416622781852	25.2014407610311
14	18.7265165296558	28.7551355422169	25.951194263737985	26.567153664389316
15	19.4064909934969	31.725417007629115	28.902349809861583	19.965742189012406
16	19.528000258505575	32.5713532155561	25.900337367242976	22.000309158695348
17	21.57223207629175	33.11799935315384	24.219818236811484	21.089950333742923
18	20.366571386402082	29.935295006388408	26.961985998196862	22.736147609012647
19	22.968831738196734	26.72790454157617	27.236036841702305	23.067226878524792
20	22.398547478129643	27.453263036118873	26.630353619907726	23.517835865843757
21	19.84498980969856	30.158139621539686	27.283720640476677	22.71314992828508
22	21.933441218655837	31.03394370342424	24.290325050383842	22.742290027536082
23	23.352922117335332	29.298987024941418	28.113209140035206	19.23488171768804
24	22.177944409074595	30.038639014721134	25.37415625439394	22.409260321810333
25	20.89936981211936	30.68656383003739	27.762156982622805	20.65190937522045
26	22.383247501108784	30.454480959702728	26.541150238072504	20.621121301115984
27	22.14778731230956	29.12540416273345	27.424510723494105	21.30229780146289
28	22.6603355322803	29.11548318795507	25.07149289450498	23.152688385259644
29	21.736837078322814	29.652644908447257	26.810365810449	21.800152202780936
30	22.09916775418273	29.722250267049073	28.639439502322734	19.539142476445463
31	23.558716860719965	29.36910465993196	26.518055988913886	20.554122490434185
32	20.547002230839496	29.91733416131365	27.451384368260367	22.084279239586486
33	20.570425784339573	26.835486422356976	30.709267070920117	21.88482072238334
34	22.30926442929985	29.48719860034541	26.90329734477096	21.300239625583785
35	19.87342955534995	27.10212435669284	27.400950908539844	25.623495179417365
36	20.232548505236235	27.055621743014623	28.692608735452772	24.01922101629637
37	20.547345566018198	30.504884424391204	25.988944460128522	22.958825549462077
38	22.620942789254148	27.29797541834898	26.00927092520972	24.071810867187146
39	20.948848661489414	29.000261390815833	26.678860390217213	23.37202955747754
40	18.06140344914315	30.601730689488747	28.07523672922799	23.26162913214011
41	21.45398687581992	30.179641822095967	25.31909476739186	23.04727653469226
42	20.31065529558648	30.211704129257512	27.281917040401034	22.195723534754983
43	19.941226657864362	27.862300087942486	28.023269022871606	24.17320423132154
44	22.96752609799533	27.59711536818599	26.012388113464603	23.422970420354083
45	19.58579953965746	27.084013232332087	29.300759522060353	24.029427705950095
46	19.061926079083737	30.560226970143304	26.177304002280337	24.20054294849262
47	19.755330243784393	29.816039108658927	27.265549410459762	23.163081237096925
48	23.244933864711495	28.924893495325094	25.34259931700126	22.487573322962152
49	18.81592595422072	30.257596079706957	24.48962393508393	26.4368540309884
50	20.743587352445427	27.12262148503124	27.40502990411476	24.728761258408575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	134718.0
1	136358.5
2	100590.5
3	61235.5
4	56785.5
5	41312.5
6	21673.0
7	15651.0
8	15988.0
9	14411.0
10	12187.5
11	11162.5
12	10604.0
13	9238.5
14	8021.5
15	8297.5
16	10029.0
17	11155.0
18	11234.5
19	11587.0
20	12331.5
21	15493.0
22	19614.0
23	25211.5
24	33428.5
25	41991.5
26	50849.5
27	56478.5
28	59377.0
29	65322.5
30	74555.5
31	89375.0
32	111652.5
33	125134.5
34	133327.5
35	145776.0
36	154783.5
37	162468.5
38	171735.0
39	190452.0
40	206237.0
41	215373.0
42	232296.0
43	237031.5
44	231681.0
45	236271.0
46	257965.5
47	286241.5
48	300753.0
49	314483.0
50	343169.0
51	343968.0
52	308811.0
53	260251.5
54	214798.5
55	193910.5
56	177979.5
57	160307.0
58	143772.5
59	123281.5
60	104691.0
61	85847.5
62	64716.5
63	45146.0
64	29875.5
65	22544.5
66	15881.0
67	10749.5
68	8674.5
69	6671.5
70	4737.5
71	3368.0
72	2552.0
73	1830.0
74	1120.5
75	688.5
76	395.5
77	202.0
78	62.5
79	49.5
80	59.0
81	55.5
82	52.0
83	27.0
84	2.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	32969.0
25	40369.0
26	33305.0
27	40397.0
28	42419.0
29	36522.0
30	42664.0
31	64961.0
32	67123.0
33	15941.0
34	12702.0
35	12575.0
36	14223.0
37	15285.0
38	10242.0
39	11252.0
40	12016.0
41	13877.0
42	20076.0
43	28462.0
44	85473.0
45	224759.0
46	355038.0
47	361480.0
48	571298.0
49	551238.0
50	718463.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.93830443421194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.74575421877618	5.933545521534268
2	9.984722467470435	1.5852353327579227
3	3.962089010404321	0.9435680628010504
4	2.082613362905074	0.6612967557399355
5	1.2509247813654578	0.49651108693895074
6	0.8352870226923712	0.3978457605647124
7	0.6423142312057902	0.356922013681685
8	0.4806355850841277	0.3052345277050705
9	0.3605257747490903	0.25757670207045585
>10	3.136563583123263	5.053307965892415
>50	0.544941799351839	3.103057430724456
>100	1.291940669180994	27.191301398492673
>500	0.5151434832563712	28.70109395473488
>1k	0.1596046766665936	18.61283591721146
>5k	0.0032655688320530657	1.8499357636526375
>10k+	0.003673764936059699	4.550731805497444
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	25387	0.7390406590261966	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	16965	0.49386791587739504	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	16524	0.4810299700535264	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	15568	0.4531998652743463	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	14603	0.4251077616008016	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	14417	0.4196931177839318	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	13862	0.40353651929811074	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	11657	0.3393467901787677	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11455	0.3334663705496941	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	9829	0.28613190363447777	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	7741	0.2253481601418753	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	7607	0.22144728771466807	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	7220	0.21018133525698746	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7152	0.20820178805512107	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	6582	0.19160852474535892	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	5685	0.16549596827368057	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	5274	0.15353135209769414	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	4859	0.14145029196865677	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	4819	0.14028585243814715	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	4798	0.1396745216846296	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4525	0.1317272218889014	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	4433	0.12904901096872928	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	4383	0.12759346155559223	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4264	0.12412925395232612	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4236	0.12331414628096937	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	4231	0.12316859133965567	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4129	0.12019927053685611	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	3996	0.1163275090979116	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3992	0.11621106514486065	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	3655	0.10640066210031705	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT	3504	0.10200490287264322	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	3478	0.10124801717781196	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.1177539475227859	0.0	0.0	0.0	0.0
2	0.18462188756230116	0.0	0.0	0.0	0.0
3	0.24275653112299422	0.0	0.0	0.0	0.0
4	0.3229573037868447	0.0	0.0	0.0	0.0
5	0.3791997331104596	0.0	0.0	0.0	0.0
6	0.47887575692208356	0.0	0.0	0.0	0.0
7	0.5405910520390937	0.0	0.0	0.0	0.0
8	0.6067021063837778	0.0	0.0	0.0	0.0
9	0.6806440165711389	0.0	0.0	0.0	0.0
10	0.7368864458947538	0.0	0.0	0.0	0.0
11	0.7990675168239679	0.0	0.0	0.0	0.0
12	0.8533886209222419	0.0	0.0	0.0	0.0
13	0.9085248326918727	0.0	0.0	0.0	0.0
14	0.9597601720342962	0.0	0.0	0.0	0.0
15	1.0146926068860878	0.0	0.0	0.0	0.0
16	1.0640066210031704	0.0	0.0	0.0	0.0
17	1.1272939094863685	0.0	0.0	0.0	0.0
18	1.17011617322086	0.0	0.0	0.0	0.0
19	1.2194301873379427	0.0	0.0	0.0	0.0
20	1.267783538842355	0.0	0.0	0.0	0.0
21	1.3033862774876868	0.0	0.0	0.0	0.0
22	1.340182566651791	0.0	0.0	0.0	0.0
23	1.378463516217295	0.0	0.0	0.0	0.0
24	1.418636680019877	0.0	0.0	0.0	0.0
25	1.4605856141064864	0.0	0.0	0.0	0.0
26	1.5008461108738567	0.0	0.0	0.0	0.0
27	1.5348477451647375	0.0	0.0	0.0	0.0
28	1.5696644871269754	0.0	0.0	0.0	0.0
29	1.6077998817511656	0.0	0.0	0.0	0.0
30	1.6473617148002302	0.0	0.0	0.0	0.0
31	1.6812177941497977	0.0	0.0	0.0	0.0
32	1.71385121199233	0.0	0.0	0.0	0.0
33	1.7453493012926151	0.0	0.0	0.0	0.0
34	1.7825822552806605	0.0	0.0	0.0	0.0
35	1.816525667595016	0.0	0.0	0.0	0.0
36	1.842026893313177	0.0	0.0	0.0	0.0
37	1.8610363686487466	0.0	0.0	0.0	0.0
38	1.8765816363810501	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAAC	95	0.0	186.95424	44
GGGCACC	15	4.4668326E-5	186.95422	44
GACGTAT	125	0.0	179.47606	44
GATTGAC	85	0.0	175.95694	44
ACGAACA	295	0.0	174.27937	44
CCCGGGC	120	0.0	171.37471	44
GACCAAT	115	0.0	170.69734	44
GTTCATG	55	0.0	169.9584	44
GTACAGG	100	0.0	158.9111	44
CTACGAC	60	0.0	155.7952	44
GATCCCA	105	0.0	151.34392	44
AATGTAC	100	0.0	140.21568	44
TCGTAGG	60	0.0	140.21567	44
TAGGCAT	240	0.0	140.21567	44
TCTCGCC	130	0.0	136.6204	44
GCTATAC	70	0.0	133.53874	44
CATGACC	190	0.0	132.8359	44
CGCTACG	105	0.0	124.63616	44
CTTCGGG	45	1.8644641E-9	124.636154	44
GTGGGTT	30	4.5228353E-6	124.636154	44
>>END_MODULE