FastQCFastQC Report
Tue 24 May 2016
ERR841082.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841082.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887339
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC151640.8034592619555893No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT114450.6064093414060748No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA113230.5999452138698983No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA89610.47479546599736455No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG83960.44485913765359586No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA82620.4377591942941888No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG63260.3351809081463372No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC62240.32977647364887813No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA50710.26868516996681574No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT49120.2602606103090118No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT45590.24155702817564836No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC43700.23154292895976822No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAGCACTGCA39470.20913042119089362No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC37050.1963081354224122No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA35630.18878431484751812No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC34720.1839627115213536No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG32030.16970984015060359No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA28970.1534965366582262No Hit
CCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACG28690.15201296640402173No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG27760.1470853937739855No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC27280.14454213048106354No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA26640.14115111275716763No Hit
TGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACT24970.1323026758838767No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT24390.12922956607159605No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT24320.12885867350804492No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA23340.12366617761832929No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC22970.12170574549670198No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT22320.11826174312087018No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA22000.11656623425892222No Hit
AATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTT21580.11434087887761551No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA21210.1123804467559882No Hit
CTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAG19170.10157157776106995No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC18890.10008800750686549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATTC1050.0191.439544
CGTACCT650.0191.439544
CAGGTCA154.0625393E-5191.4394844
GGACTAT154.0625393E-5191.4394844
GTCTCAT100.0030881523191.4394844
GCGGCAT100.0030881523191.4394844
GAGTTAT1750.0191.4394844
TTAGGTA1000.0191.4394744
GCTCCAT1100.0182.7376944
TAGATAA950.0181.3637244
GAGCTAT900.0180.8039744
CGCATCT600.0175.4861944
ATAGATG600.0175.4861944
CTAGATG550.0174.035944
CCATTGA1050.0173.2071744
GCATGTC450.0170.1684444
TGCAATC450.0170.1684444
ACGGCAC1300.0169.3503344
CTGGTTT800.0167.5095444
GACGGCA650.0161.9872644
Produced by FastQC (version 0.11.5)