FastQCFastQC Report
Tue 24 May 2016
ERR841081.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841081.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10590137
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA1033960.9763424212547958No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC765990.7233050903874048No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC757120.7149293724906486No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT537510.5075571732452564No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA416640.39342267243568235No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT387850.36623699957800354No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT330700.3122716920470434No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA270870.25577572792495507No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT223940.21146090933478953No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG222200.20981787110025113No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA182310.17215074743603412No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA179420.16942179312694444No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT171440.16188647984440618No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA163760.15463444901609866No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT146920.13873286058527853No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA145650.13753363152903497No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA143960.13593780703686836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA134200.12672168452589422No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC127880.1207538674900995No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC125060.11809101242033035No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA116450.10996080598390748No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG112730.10644810355144602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC700.0160.721544
AGATCCG1550.0151.2164644
CTTGATA451.0913936E-11145.8398944
CTCACGG1200.0140.6313244
CCGGCTA700.0133.9345744
CGTGCGC1800.0130.2141944
GCACTCG3450.0108.7005344
CTTACGC703.6379788E-12107.1476644
TTACCCG1950.0105.7739744
ATGGGTT1350.0104.1713444
TCGTGCG3400.0100.086943
CTATCAT270900.099.6030344
GTGTGTA850.099.26916544
ACTTCTA265250.098.0133544
ATACGGC550.095.1875443
GAACACA102050.094.4432444
CGCTTAA950.093.6845843
GTTGTAC1350.090.2818344
ACGAACA2350.089.1117443
CAGTCGT1000.089.0003543
Produced by FastQC (version 0.11.5)