FastQCFastQC Report
Tue 24 May 2016
ERR841080.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841080.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14072955
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA600650.42681156871460185No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA414460.2945081541154647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA391910.27848451160399507No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA369150.262311646701066No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA318730.22648406109448938No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT313480.2227535013080053No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA298690.2122439814523673No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAATA283840.20169182662774093No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG263230.18704671478022916No Hit
AAAAAAAAAAAAAAAAAAAAAAAA225420.1601795784893791No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA209660.14898079330176214No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC207050.1471261721507672No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC160370.11395616627780022No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAA151630.10774567246182483No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG144620.10276448691834801No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT143390.10189047005408601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA650.0137.626544
GTTACGG253.6529327E-6130.119644
CTAGTAC1500.0124.6979544
ATCGACC1750.0111.5310844
TGCGATA1800.099.3969144
CTACTCG1150.099.0040444
CATACGG600.096.04040543
CGGTTCA456.227965E-790.3608344
CTCGAGA3200.088.9489444
TGTACGG502.7284841E-1183.81708543
GAATACA103150.082.62562644
AGCGTAC1300.080.59334643
ATATCGG13700.078.95029444
TTCGCAC950.077.19993643
CGGGCAA556.366463E-1176.1973443
TCGTAAC351.9111958E-674.8366843
CCTTAGC158750.073.4554443
CTATCGA2050.071.407144
CCTATCG1350.069.84756543
TGGCCGA6550.066.3818443
Produced by FastQC (version 0.11.5)