##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841080.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14072955
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.551968083462214	39.0	37.0	39.0	35.0	39.0
2	38.045320758859816	39.0	38.0	39.0	35.0	39.0
3	38.14362974940231	39.0	38.0	39.0	35.0	39.0
4	38.4874086501378	39.0	38.0	40.0	35.0	41.0
5	39.32730339861102	40.0	39.0	41.0	36.0	41.0
6	39.31423293828482	40.0	39.0	41.0	36.0	41.0
7	39.329124196019954	40.0	39.0	41.0	36.0	41.0
8	39.25212139170487	40.0	39.0	41.0	36.0	41.0
9	39.19364476046431	40.0	39.0	41.0	36.0	41.0
10	39.21354115038384	40.0	39.0	41.0	36.0	41.0
11	39.09866485041699	40.0	38.0	41.0	35.0	41.0
12	39.042144382611895	40.0	38.0	41.0	35.0	41.0
13	38.99132918424027	40.0	38.0	41.0	35.0	41.0
14	38.99819085614926	40.0	38.0	41.0	35.0	41.0
15	38.923119842279036	40.0	38.0	41.0	35.0	41.0
16	38.9283512240322	40.0	38.0	41.0	35.0	41.0
17	38.983509859869514	40.0	38.0	41.0	35.0	41.0
18	38.999048600667024	40.0	38.0	41.0	35.0	41.0
19	38.97296004996819	40.0	38.0	41.0	35.0	41.0
20	39.00696143773642	40.0	38.0	41.0	35.0	41.0
21	39.04322212356964	40.0	38.0	41.0	35.0	41.0
22	39.053228337616375	40.0	38.0	41.0	35.0	41.0
23	39.04237013477269	40.0	38.0	41.0	35.0	41.0
24	38.98298395752705	40.0	38.0	41.0	35.0	41.0
25	38.96312037649342	40.0	38.0	41.0	35.0	41.0
26	38.94330365398876	40.0	38.0	41.0	35.0	41.0
27	38.89796313903339	40.0	38.0	41.0	35.0	41.0
28	38.885795786209044	40.0	38.0	41.0	35.0	41.0
29	38.88332750535159	40.0	38.0	41.0	35.0	41.0
30	38.90606835142662	40.0	38.0	41.0	35.0	41.0
31	38.821001930691104	40.0	38.0	41.0	35.0	41.0
32	38.740768565923865	40.0	38.0	41.0	35.0	41.0
33	38.58041163933911	40.0	38.0	41.0	35.0	41.0
34	38.611184354056604	40.0	38.0	41.0	35.0	41.0
35	38.631096511294665	40.0	38.0	41.0	35.0	41.0
36	38.62676559910423	40.0	37.0	41.0	35.0	41.0
37	38.59964426099402	40.0	37.0	41.0	35.0	41.0
38	38.5385064669403	40.0	37.0	41.0	35.0	41.0
39	38.483697155143844	40.0	37.0	41.0	34.0	41.0
40	38.39862289459739	40.0	37.0	41.0	34.0	41.0
41	38.33955879699485	40.0	37.0	41.0	34.0	41.0
42	38.244173440925444	40.0	36.0	41.0	34.0	41.0
43	38.14402998745574	40.0	36.0	41.0	34.0	41.0
44	38.01735166331955	39.0	36.0	41.0	34.0	41.0
45	37.84628818048477	39.0	36.0	41.0	34.0	41.0
46	37.54789220641615	39.0	35.0	41.0	33.0	41.0
47	37.35710071732803	39.0	35.0	41.0	33.0	41.0
48	37.221171172344356	39.0	35.0	40.0	32.0	41.0
49	37.46430877573717	39.0	35.0	40.0	33.0	41.0
50	36.86567498373605	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	18.0
21	68.0
22	141.0
23	322.0
24	710.0
25	1478.0
26	3051.0
27	6711.0
28	13962.0
29	28930.0
30	57281.0
31	104581.0
32	180602.0
33	313963.0
34	531292.0
35	558936.0
36	899068.0
37	1384097.0
38	1935211.0
39	3129073.0
40	4923453.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	55.99430965280568	15.10955588218679	28.896134465007528
2	26.94913754787108	26.64812756098488	29.02505550540025	17.37767938574379
3	25.3562240481832	25.472375915363905	25.024630576876	24.1467694595769
4	22.78156932925601	25.880229134535	29.364202472046557	21.97399906416243
5	18.61775298791192	30.214699045083282	28.55002378675978	22.617524180245017
6	21.63098652699451	31.17160539488686	26.761835023276916	20.435573054841715
7	25.996373895887537	29.860537463524896	23.01776705745169	21.125321583135882
8	23.810734845666744	26.539003357859098	26.882946758516603	22.76731503795756
9	22.38789223727355	30.684962753025218	25.68457726184728	21.24256774785395
10	25.64008056587973	24.46265905064004	26.201952610521385	23.69530777295884
11	23.03033016164693	28.19995516222428	25.83988934804382	22.92982532808497
12	22.949131863208542	26.727449920787784	27.36297387435688	22.960444341646795
13	21.856447348833278	27.89517198058261	25.533500249236923	24.71488042134719
14	21.39375134788678	28.27733763093821	25.586431563236005	24.742479457939005
15	20.449386784793955	32.68311452711957	26.871570327624866	19.99592836046161
16	22.645876434622295	33.853600754070484	22.444369359526835	21.056153451780382
17	22.292219366863606	35.65979568612278	21.80608123880166	20.241903708211957
18	22.367164536517027	29.15453080038983	23.60863798683361	24.869666676259534
19	23.268140912836003	28.19360255184501	24.385624767506183	24.152631767812803
20	24.62718029013807	27.39701079126594	25.87375572507693	22.10205319351906
21	21.77663468688701	30.933332764867078	24.850971242358124	22.439061305887783
22	23.805632861044465	28.548098107327142	24.10755949976391	23.538709531864487
23	25.670706685269725	27.75852690497483	25.415777994031814	21.154988415723636
24	22.594437344537806	29.66865878559265	25.40591510453917	22.330988765330382
25	22.791434754726563	29.00751761116452	26.05348771129219	22.147559922816733
26	22.894973184451832	31.911238470750675	25.262462003077456	19.931326341720037
27	23.27029267803754	28.571718660652067	25.866840023711333	22.291148637599058
28	24.022346857444923	28.898272050163715	25.034258566755852	22.045122525635513
29	22.95395389663368	30.52824008158243	25.489048772672618	21.028757249111266
30	24.114795251654588	30.181732166580893	25.15816425413428	20.545308327630234
31	22.334190644723268	29.77445177123747	25.82894290937954	22.06241467465972
32	21.66907206050884	27.6641308623489	28.432530984403375	22.234266092738885
33	21.815520073359064	27.064857778105644	29.932757305324415	21.186864843210877
34	22.001068638537625	29.19773501139902	25.96020407589178	22.84099227417158
35	21.374860480406095	28.58246524408627	26.139011109425308	23.90366316608232
36	21.868676372466446	27.880119583276315	27.33965611820837	22.91154792604887
37	20.908959943089343	30.61739636126159	24.866667019645067	23.606976676004
38	22.16069577802597	28.30686642589005	26.50276666307121	23.029671133012773
39	21.845456456371206	30.523401668273742	26.184237680684202	21.44690419467085
40	19.751888314290273	29.703500285609884	27.315268661353105	23.229342738746737
41	21.125037296064637	30.644414941434135	26.508419953125458	21.722127809375777
42	21.849492655692043	30.14468040200932	25.9293204097803	22.07650653251834
43	22.180798338205708	27.607510136740732	26.441117661156287	23.77057386389727
44	21.751921435615415	29.922888685358267	24.441714262173882	23.883475616852433
45	20.253117646438106	28.42394504416094	29.07473698650374	22.248200322897212
46	20.101254222179925	31.34502969646799	26.060144474016	22.493571607336087
47	21.53006291278479	30.493990362428473	26.682080179789224	21.29386654499751
48	22.47837015501208	30.399108327383352	25.16789864479306	21.954622872811512
49	20.11534537098899	31.138082163054072	24.559735502676077	24.186836963280868
50	19.696044647484822	33.371831212227825	25.627318682676446	21.304805457610907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	310597.0
1	312149.0
2	222879.5
3	130441.0
4	123882.5
5	88528.5
6	41406.5
7	25937.5
8	27131.0
9	27005.5
10	26901.5
11	27568.5
12	27036.0
13	25418.0
14	28658.0
15	33658.5
16	35574.5
17	37519.0
18	41590.5
19	48339.5
20	56774.5
21	69737.0
22	84874.0
23	106239.5
24	129217.0
25	144298.0
26	154755.0
27	172445.0
28	197751.5
29	235688.5
30	295879.5
31	390761.5
32	508428.0
33	551791.5
34	562138.5
35	622849.0
36	662064.0
37	677895.5
38	715843.0
39	778546.5
40	851011.0
41	929917.5
42	1001814.0
43	1070156.5
44	1117776.0
45	1092856.0
46	1102182.5
47	1152859.5
48	1212038.0
49	1294465.0
50	1416056.5
51	1445145.0
52	1273313.0
53	1088409.5
54	977230.5
55	861332.5
56	738578.5
57	653826.5
58	580387.5
59	487106.5
60	384314.0
61	299062.5
62	238814.0
63	197974.0
64	166618.5
65	143894.5
66	102224.0
67	59459.5
68	37703.0
69	26312.0
70	19536.0
71	14094.0
72	9876.5
73	6306.0
74	4246.0
75	2971.0
76	2037.5
77	1464.5
78	855.0
79	459.5
80	347.5
81	296.0
82	222.0
83	188.5
84	171.5
85	143.5
86	154.0
87	109.0
88	67.0
89	62.5
90	35.0
91	8.0
92	8.5
93	9.0
94	5.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	73029.0
25	76101.0
26	85718.0
27	121100.0
28	113098.0
29	101016.0
30	144464.0
31	210201.0
32	297464.0
33	53172.0
34	44007.0
35	45788.0
36	53576.0
37	73542.0
38	40291.0
39	44322.0
40	48372.0
41	52857.0
42	68883.0
43	115943.0
44	364136.0
45	833791.0
46	1640221.0
47	1308814.0
48	2703146.0
49	1912094.0
50	3447809.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.930329577007052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.40458316940466	7.586149681963896
2	12.725382313411792	2.781852453580147
3	5.236849336581599	1.7172146758190283
4	2.6096246005606907	1.1409622782557491
5	1.6316662927460184	0.8917325169703626
6	1.0549931120749652	0.6918853449871025
7	0.7584647138795071	0.5803188506663357
8	0.569686119919667	0.4981485636934658
9	0.47096241161777147	0.4632996939627867
>10	3.6856623469675602	7.837968459514491
>50	0.4309421386125374	3.3491530437309702
>100	1.1230893096066084	34.3003676098024
>500	0.23979316739478904	16.48384118115006
>1k	0.050279212518262215	10.318968032373684
>5k	0.005156842309565356	3.5607332802062928
>10k+	0.0028649123942029757	7.797404333323199
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	60065	0.42681156871460185	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	41446	0.2945081541154647	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	39191	0.27848451160399507	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	36915	0.262311646701066	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31873	0.22648406109448938	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	31348	0.2227535013080053	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	29869	0.2122439814523673	No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAATA	28384	0.20169182662774093	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	26323	0.18704671478022916	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	22542	0.1601795784893791	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20966	0.14898079330176214	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	20705	0.1471261721507672	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	16037	0.11395616627780022	No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAA	15163	0.10774567246182483	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG	14462	0.10276448691834801	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	14339	0.10189047005408601	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.08361428001439641	0.0	0.0	0.0	0.0
2	0.14697694975930783	0.0	0.0	7.105828164731572E-6	0.0
3	0.19062094634708915	0.0	0.0	7.105828164731572E-6	0.0
4	0.2590927065424426	0.0	0.0	7.105828164731572E-6	0.0
5	0.3017418871871615	0.0	0.0	7.105828164731572E-6	0.0
6	0.41143455656612277	0.0	0.0	7.105828164731572E-6	0.0
7	0.4586954196897524	0.0	0.0	7.105828164731572E-6	0.0
8	0.5165866017478206	0.0	0.0	7.105828164731572E-6	0.0
9	0.5851791610219744	0.0	0.0	7.105828164731572E-6	0.0
10	0.6339393538883624	0.0	0.0	7.105828164731572E-6	0.0
11	0.6996966877247884	0.0	0.0	7.105828164731572E-6	0.0
12	0.7556195553812259	0.0	0.0	7.105828164731572E-6	0.0
13	0.8146903049146395	0.0	0.0	7.105828164731572E-6	0.0
14	0.8669820943788991	0.0	0.0	7.105828164731572E-6	0.0
15	0.9263655003515608	0.0	0.0	7.105828164731572E-6	0.0
16	0.9781527760161245	0.0	0.0	7.105828164731572E-6	0.0
17	1.0444430469649053	0.0	0.0	7.105828164731572E-6	0.0
18	1.0867369362013877	0.0	0.0	7.105828164731572E-6	0.0
19	1.1317736751094565	0.0	0.0	7.105828164731572E-6	0.0
20	1.1855719001446392	0.0	0.0	7.105828164731572E-6	0.0
21	1.2285550547131003	0.0	0.0	7.105828164731572E-6	0.0
22	1.2678076494950776	0.0	0.0	7.105828164731572E-6	0.0
23	1.3073657948881383	0.0	0.0	7.105828164731572E-6	0.0
24	1.3460783467295958	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
25	1.3838742467378031	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
26	1.4262534059122622	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
27	1.4625357645213817	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
28	1.5000687488874938	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
29	1.5373246059551815	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
30	1.5720507881962247	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
31	1.6044107296584122	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
32	1.6392577109782558	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
33	1.674722899348431	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
34	1.7076655187201266	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
35	1.7398407086500312	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
36	1.7658906747019372	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
37	1.7849129766989236	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
38	1.801526402948066	0.0	0.0	7.105828164731572E-6	7.105828164731572E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	65	0.0	137.6265	44
GTTACGG	25	3.6529327E-6	130.1196	44
CTAGTAC	150	0.0	124.69795	44
ATCGACC	175	0.0	111.53108	44
TGCGATA	180	0.0	99.39691	44
CTACTCG	115	0.0	99.00404	44
CATACGG	60	0.0	96.040405	43
CGGTTCA	45	6.227965E-7	90.36083	44
CTCGAGA	320	0.0	88.94894	44
TGTACGG	50	2.7284841E-11	83.817085	43
GAATACA	10315	0.0	82.625626	44
AGCGTAC	130	0.0	80.593346	43
ATATCGG	1370	0.0	78.950294	44
TTCGCAC	95	0.0	77.199936	43
CGGGCAA	55	6.366463E-11	76.19734	43
TCGTAAC	35	1.9111958E-6	74.83668	43
CCTTAGC	15875	0.0	73.45544	43
CTATCGA	205	0.0	71.4071	44
CCTATCG	135	0.0	69.847565	43
TGGCCGA	655	0.0	66.38184	43
>>END_MODULE