FastQCFastQC Report
Tue 24 May 2016
ERR841079.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841079.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5441185
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA213920.3931496539816235No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC180530.3317843447704866No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG121860.22395856784873147No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT108890.20012184845764297No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA100730.18512511520927885No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC93410.17167216332471696No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75610.13895870109176586No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT74190.1363489754529574No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC71890.13212195505207044No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT69360.12747223261109483No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC62300.11449711781532884No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC62120.1141663075230855No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA60160.11056415100754707No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT59300.10898361294460675No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58840.10813820886442933No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT56800.10438902555233832No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATT56750.10429713380449294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAT1300.0169.882944
ACCGATT1150.0161.314944
AAACGGC950.0148.7816644
CACGCAT1050.0143.0252244
ATAGCTC900.0137.416444
CGACCGG1150.0130.5882644
GTCACAA1000.0123.6747644
TAGGCAT1000.0123.6747644
AACCGAT1100.0120.46242544
GTTCCAC1700.0119.5176344
CATACGT1950.0113.25526444
ATGACGC1100.0112.431644
CTATCAT52550.0112.1259544
GTATGCG651.8189894E-12108.7250644
TACCGAT1050.0104.9826643
CTAGATA1400.0104.9826543
CCGATGT306.15546E-9104.9826543
TATGACG700.0104.9826543
GAAACCG950.0102.2873944
TGGTCAT1650.0101.7238344
Produced by FastQC (version 0.11.5)