##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841079.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5441185
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.3208486754264	39.0	37.0	39.0	34.0	39.0
2	37.83587766267826	39.0	38.0	39.0	35.0	39.0
3	37.99066306328493	39.0	38.0	39.0	35.0	39.0
4	38.445588782590555	39.0	38.0	40.0	35.0	41.0
5	39.14975800308205	40.0	38.0	41.0	35.0	41.0
6	39.154133520547454	40.0	38.0	41.0	35.0	41.0
7	39.072740037326426	40.0	38.0	41.0	35.0	41.0
8	38.998921742230785	40.0	38.0	41.0	35.0	41.0
9	38.94861762649129	40.0	38.0	41.0	35.0	41.0
10	38.93772716788714	40.0	38.0	41.0	35.0	41.0
11	38.830990308177356	40.0	38.0	41.0	35.0	41.0
12	38.68952222723543	40.0	38.0	41.0	35.0	41.0
13	38.52067647029094	40.0	38.0	41.0	34.0	41.0
14	38.532304635846785	40.0	38.0	41.0	34.0	41.0
15	38.512031294653646	40.0	38.0	41.0	35.0	41.0
16	38.524434291427326	40.0	38.0	41.0	35.0	41.0
17	38.66613761524374	40.0	38.0	41.0	35.0	41.0
18	38.70844567865272	40.0	38.0	41.0	35.0	41.0
19	38.688500574782886	40.0	38.0	41.0	35.0	41.0
20	38.722107959938874	40.0	38.0	41.0	35.0	41.0
21	38.73618577570878	40.0	38.0	41.0	35.0	41.0
22	38.69715457202797	40.0	38.0	41.0	35.0	41.0
23	38.66443541250665	40.0	38.0	41.0	35.0	41.0
24	38.6100136275462	40.0	38.0	41.0	35.0	41.0
25	38.545288261615966	40.0	37.0	41.0	35.0	41.0
26	38.49258490100757	40.0	37.0	41.0	35.0	41.0
27	38.42943530229626	40.0	37.0	41.0	34.0	41.0
28	38.38132425347631	40.0	37.0	41.0	34.0	41.0
29	38.285309046575776	40.0	37.0	41.0	34.0	41.0
30	38.30211743087862	40.0	37.0	41.0	34.0	41.0
31	38.1873946701062	40.0	37.0	41.0	34.0	41.0
32	37.95266296775085	40.0	36.0	41.0	34.0	41.0
33	37.8379714838501	39.0	36.0	41.0	33.0	41.0
34	37.819344536091535	39.0	36.0	41.0	33.0	41.0
35	37.82687372925491	39.0	35.0	41.0	33.0	41.0
36	37.82647120134871	39.0	35.0	41.0	34.0	41.0
37	37.793163432329486	39.0	35.0	41.0	34.0	41.0
38	37.69354975883968	39.0	35.0	41.0	34.0	41.0
39	37.65239989270829	39.0	35.0	41.0	34.0	41.0
40	37.593955349824796	39.0	35.0	41.0	34.0	41.0
41	37.53397199872957	39.0	35.0	41.0	34.0	41.0
42	37.47347439187281	39.0	35.0	41.0	34.0	41.0
43	37.339920007409276	38.0	35.0	41.0	34.0	41.0
44	37.14428100672375	38.0	35.0	40.0	33.0	41.0
45	36.98512579086053	38.0	35.0	40.0	33.0	41.0
46	36.72365868390752	37.0	35.0	40.0	32.0	41.0
47	36.46519477449373	37.0	35.0	40.0	32.0	41.0
48	36.314694099150636	37.0	35.0	40.0	31.0	41.0
49	36.50633962770152	37.0	35.0	40.0	32.0	41.0
50	36.26574137481249	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	6.0
21	33.0
22	63.0
23	151.0
24	332.0
25	779.0
26	1563.0
27	3314.0
28	7255.0
29	14977.0
30	29677.0
31	55107.0
32	92751.0
33	159215.0
34	273535.0
35	289237.0
36	472204.0
37	673963.0
38	862930.0
39	1186342.0
40	1317747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.35353236473305	19.090437101476976	31.556030533789976
2	24.768520092590123	20.324892463682083	34.97155490945447	19.935032534273326
3	22.199741416621563	21.2092035098972	28.895525515122166	27.695529558359073
4	19.775085757973677	22.67178932530322	33.98426997060383	23.568854946119274
5	16.17570437322017	25.6262928020275	32.34800875177007	25.849994072982263
6	21.014815706505107	26.801863932213294	28.52924500821053	23.654075353071068
7	22.710604399593105	27.13881259321269	25.141949777484136	25.00863322971007
8	23.484755618491192	25.93795285401985	27.28104264052775	23.296248886961205
9	22.917158670399925	25.67016192244888	28.159987208668703	23.2526921984825
10	24.730697449176972	22.162010664956256	29.056042020258456	24.05124986560832
11	25.579703685869898	23.71198553256322	26.444184492899986	24.264126288666898
12	24.456382203508976	25.924922604175375	27.112972633718574	22.50572255859707
13	23.684693683453144	24.514549679895094	27.935569182080744	23.865187454571018
14	20.39936153613597	25.164481634055818	27.562451929129406	26.873704900678803
15	22.623252104091296	29.309350812368994	26.18986489156314	21.877532191976563
16	21.09733449607025	31.74064105521132	25.573455047016413	21.588569401702017
17	22.29617261681049	32.98911909813763	24.160490775446892	20.554217509604985
18	21.3110011881603	26.734397010945226	26.805245548534007	25.149356252360473
19	23.958090011642685	24.655879188081272	26.51345249242582	24.872578307850223
20	24.390550955352555	22.894240868487287	29.564276899241616	23.150931276918538
21	23.904351717502713	28.76812679590935	26.067060759742596	21.26046072684535
22	24.34741696891394	26.256284246905775	25.504903803123767	23.891394981056514
23	26.681908444575953	25.02714022772613	26.761872643550994	21.529078684146928
24	23.18739392246358	27.75669270572495	26.03543162013422	23.020481751677256
25	24.32954024087349	27.909459649500985	26.333251267983478	21.42774884164205
26	24.957734537881247	30.269315061437542	25.777772507782863	18.995177892898347
27	25.00013025359258	28.073928961923528	25.749609425298818	21.176331359185074
28	22.880985311387146	27.00610881387852	25.344768825408536	24.7681370493258
29	22.488110111233834	28.40363154622873	25.255300657263398	23.85295768527404
30	26.657720621929737	29.34143916838216	24.794250142264502	19.2065900674236
31	24.95060655457535	27.66688202055595	25.724673501278712	21.657837923589987
32	23.11516128283441	27.723298000318746	28.011611341911337	21.149929374935507
33	22.171336497357434	25.505322069567267	30.1526638300931	22.1706776029822
34	24.057505467350328	26.35360194879907	27.20576001939487	22.383132564455728
35	20.537276264215013	25.59818737937495	29.849570385371337	24.014965971038702
36	21.124320232825948	27.296515099078533	27.980765144109633	23.59839952398589
37	20.657460995146458	31.470172615493254	26.21891692686173	21.653449462498557
38	24.005285246287734	25.230038647801262	27.085537904448504	23.6791382014625
39	21.23657596604098	29.033018059608466	26.61015039515078	23.120255579199775
40	19.018314930789366	28.854899186824735	28.265425910251835	23.861359972134068
41	20.0674529975719	28.63210079878005	26.272673569908516	25.027772633739538
42	19.86684891627758	30.377906161395618	26.043951409245324	23.71129351308148
43	21.95040007793957	25.927952311353526	27.591443623537664	24.53020398716924
44	22.71285713983754	27.73992335642223	24.609796258288437	24.937423245451797
45	20.915819649179955	28.46764771330318	28.189559182212438	22.426973455304434
46	21.05374032032299	28.3833639056192	27.668230707055102	22.894665067002713
47	21.54292395929531	28.27900407842236	28.283422737536068	21.89464922474626
48	22.65287807932274	29.1469922909441	25.525317752334864	22.67481187739829
49	19.93315740808179	30.559142284812722	25.31722255476843	24.190477752337056
50	21.209971955912085	27.693047674949455	29.572327659296942	21.52465270984152
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	48227.0
1	47864.0
2	60571.0
3	71701.0
4	64261.0
5	39610.0
6	14686.0
7	10483.0
8	13611.5
9	15660.5
10	16026.0
11	15168.0
12	13713.5
13	11940.0
14	11070.5
15	11385.5
16	11912.0
17	12892.0
18	15575.0
19	20684.0
20	25141.5
21	30003.0
22	35296.5
23	40062.0
24	44218.0
25	47313.5
26	52879.0
27	64262.0
28	79117.0
29	92583.0
30	108799.5
31	141256.0
32	191820.5
33	221349.5
34	228860.0
35	243599.5
36	252890.0
37	258665.0
38	269185.5
39	274819.5
40	288201.5
41	305720.0
42	311205.5
43	328696.0
44	353312.5
45	369066.5
46	392663.5
47	405861.0
48	435744.5
49	482983.5
50	524681.5
51	556747.5
52	527912.5
53	456440.0
54	414205.5
55	401411.5
56	381631.0
57	350072.0
58	315685.0
59	268709.5
60	215701.5
61	169411.0
62	128875.5
63	98628.5
64	74957.5
65	59289.5
66	39321.5
67	23263.0
68	17697.0
69	13853.0
70	10012.0
71	6337.5
72	3679.5
73	2308.0
74	1514.0
75	1239.0
76	1046.0
77	601.0
78	234.0
79	154.5
80	123.0
81	101.5
82	60.5
83	29.0
84	22.0
85	15.5
86	7.0
87	3.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	22739.0
25	21013.0
26	23301.0
27	28086.0
28	44062.0
29	31871.0
30	50797.0
31	92949.0
32	117977.0
33	25629.0
34	21105.0
35	20626.0
36	24206.0
37	25472.0
38	18655.0
39	18024.0
40	21678.0
41	19816.0
42	29986.0
43	52203.0
44	138208.0
45	372729.0
46	599045.0
47	580039.0
48	967740.0
49	846589.0
50	1226640.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.329792657308149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.01839491125914	8.159534818567021
2	11.508241917139296	2.6077198954266128
3	4.461649688699113	1.5164869764751319
4	2.3371484059782253	1.0591762739636614
5	1.4566051524047179	0.8251517180156093
6	0.9556573539317288	0.649643980288694
7	0.7008076726999759	0.5558003937038979
8	0.5296951346361631	0.4801068837610121
9	0.41620671232585704	0.42439821779086734
>10	3.4103518641422643	7.83964557167834
>50	0.5731469984839196	4.632164016602336
>100	1.353045011587067	39.975097369846694
>500	0.2289969797172769	16.37763326138795
>1k	0.04383656468873586	8.863550377057607
>5k	0.004579939594345538	3.343294686656522
>10k+	0.0016356927122662636	2.690595558778055
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	21392	0.3931496539816235	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	18053	0.3317843447704866	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	12186	0.22395856784873147	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	10889	0.20012184845764297	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	10073	0.18512511520927885	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	9341	0.17167216332471696	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7561	0.13895870109176586	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7419	0.1363489754529574	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC	7189	0.13212195505207044	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6936	0.12747223261109483	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	6230	0.11449711781532884	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	6212	0.1141663075230855	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	6016	0.11056415100754707	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	5930	0.10898361294460675	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5884	0.10813820886442933	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	5680	0.10438902555233832	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATT	5675	0.10429713380449294	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03587453835883176	0.0	0.0	0.0	0.0
2	0.05621937133179629	0.0	0.0	0.0	0.0
3	0.08310689675135104	0.0	0.0	0.0	0.0
4	0.11931224540242613	0.0	0.0	0.0	0.0
5	0.14553815023749422	0.0	0.0	0.0	0.0
6	0.21967641239913732	0.0	0.0	0.0	0.0
7	0.2523347395833812	0.0	0.0	0.0	0.0
8	0.29567088786725687	0.0	0.0	0.0	0.0
9	0.3407162226610564	0.0	0.0	0.0	0.0
10	0.3708934726534753	0.0	0.0	0.0	0.0
11	0.40551828324161004	0.0	0.0	0.0	0.0
12	0.44170525354311607	0.0	0.0	0.0	0.0
13	0.47743276510539523	0.0	0.0	0.0	0.0
14	0.5134727086103487	0.0	0.0	0.0	0.0
15	0.5511850819260878	0.0	0.0	0.0	0.0
16	0.5878131326172515	0.0	0.0	0.0	0.0
17	0.6373795414050432	0.0	0.0	0.0	0.0
18	0.6689719243142808	0.0	0.0	0.0	0.0
19	0.7019978184899062	0.0	0.0	0.0	0.0
20	0.7479069357134521	0.0	0.0	0.0	0.0
21	0.7857847141753129	0.0	0.0	0.0	0.0
22	0.8180387176690371	0.0	0.0	0.0	0.0
23	0.8485651563032685	0.0	0.0	0.0	0.0
24	0.8772537599805925	0.0	0.0	0.0	0.0
25	0.9069347945346464	0.0	0.0	0.0	0.0
26	0.9369650177305128	0.0	0.0	0.0	0.0
27	0.9627865988750612	0.0	0.0	0.0	0.0
28	0.990684933520915	0.0	0.0	0.0	0.0
29	1.0256221760517241	0.0	0.0	0.0	0.0
30	1.0556523992475904	0.0	0.0	0.0	0.0
31	1.0828707349593885	0.0	0.0	0.0	0.0
32	1.1103647459147226	0.0	0.0	0.0	0.0
33	1.1382447022110074	0.0	0.0	0.0	0.0
34	1.1664187121003973	0.0	0.0	0.0	0.0
35	1.1943721817949582	0.0	0.0	0.0	0.0
36	1.2178229558450961	0.0	0.0	0.0	0.0
37	1.233940768417174	0.0	0.0	0.0	0.0
38	1.2488823666168307	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAT	130	0.0	169.8829	44
ACCGATT	115	0.0	161.3149	44
AAACGGC	95	0.0	148.78166	44
CACGCAT	105	0.0	143.02522	44
ATAGCTC	90	0.0	137.4164	44
CGACCGG	115	0.0	130.58826	44
GTCACAA	100	0.0	123.67476	44
TAGGCAT	100	0.0	123.67476	44
AACCGAT	110	0.0	120.462425	44
GTTCCAC	170	0.0	119.51763	44
CATACGT	195	0.0	113.255264	44
ATGACGC	110	0.0	112.4316	44
CTATCAT	5255	0.0	112.12595	44
GTATGCG	65	1.8189894E-12	108.72506	44
TACCGAT	105	0.0	104.98266	43
CTAGATA	140	0.0	104.98265	43
CCGATGT	30	6.15546E-9	104.98265	43
TATGACG	70	0.0	104.98265	43
GAAACCG	95	0.0	102.28739	44
TGGTCAT	165	0.0	101.72383	44
>>END_MODULE