FastQCFastQC Report
Tue 24 May 2016
ERR841078.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841078.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20367816
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA2405461.1810102762122359No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT1313380.644831041285919No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG827580.4063174961910497No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC691190.3393540083040813No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG630010.30931642351835853No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG521660.2561197528492991No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG499170.24507782277687504No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA496210.24362454963261648No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC448280.2200923260500782No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG437670.2148831273809622No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA416050.20426834177999253No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC390940.19194006858663687No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT333030.1635079578487944No Hit
AGGAAAGGCACTTGCTGCTGAGGCAGG302380.14845970721652238No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG274300.13467325117233975No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC264840.13002866875859445No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA261680.12847720148296704No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA257090.12622364616805257No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT252230.1238375287757902No Hit
CGTTTGGTATTGGGTTATGGCAGGGGG221260.10863216753332808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT256700.0148.1468744
AGCGGTA3150.0135.364244
CTATACG7950.0117.3262944
ACTTCTA206250.0113.25368544
GCGTCAC951.8189894E-12112.2097944
GTACGAC4900.0108.77479644
GAACACA128550.0104.6920444
TAATCGT42500.0102.5845943
ACGGATA455.456968E-12100.0107843
CCTTAGC538600.097.095543
TCCGGGC31200.097.00564643
GAACGGT1004.080357E-879.9494844
CAATGAA275200.078.00622643
ACGACGC1300.077.0251742
CCAGAAT105250.075.0752944
GAATCAC30400.072.7610444
ATGACCG3900.071.74953544
CCCATAC65700.068.7541144
TTAATCG45950.068.5599442
ACCTTAG554100.066.4256942
Produced by FastQC (version 0.11.5)