##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841078.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20367816
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.542677280666716	38.0	35.0	39.0	27.0	39.0
2	36.73835938030862	39.0	35.0	39.0	33.0	39.0
3	36.92650036704966	39.0	35.0	39.0	33.0	39.0
4	37.37703988488506	39.0	35.0	40.0	33.0	41.0
5	37.971381909577346	40.0	35.0	41.0	34.0	41.0
6	38.0501038992104	40.0	35.0	41.0	34.0	41.0
7	38.06816052344542	40.0	35.0	41.0	34.0	41.0
8	37.930777850703286	40.0	35.0	41.0	34.0	41.0
9	37.921472680232384	40.0	35.0	41.0	34.0	41.0
10	37.884801885484436	39.0	35.0	41.0	34.0	41.0
11	37.74316853608654	39.0	35.0	41.0	34.0	41.0
12	37.69269964928984	39.0	35.0	41.0	34.0	41.0
13	37.68056702790324	39.0	35.0	41.0	34.0	41.0
14	37.618407049631635	39.0	35.0	41.0	33.0	41.0
15	37.56054114982186	39.0	35.0	40.0	33.0	41.0
16	37.682589925203565	39.0	35.0	41.0	34.0	41.0
17	37.84452073801138	39.0	35.0	41.0	34.0	41.0
18	37.85187042145314	39.0	35.0	41.0	34.0	41.0
19	37.904513817289	40.0	35.0	41.0	34.0	41.0
20	37.900591354517346	40.0	35.0	41.0	34.0	41.0
21	37.98571319575943	40.0	35.0	41.0	34.0	41.0
22	37.935341521152786	40.0	35.0	41.0	34.0	41.0
23	37.890364239347015	40.0	35.0	41.0	34.0	41.0
24	37.82256943994388	39.0	35.0	41.0	34.0	41.0
25	37.82568426939343	39.0	35.0	41.0	34.0	41.0
26	37.79132350525812	39.0	35.0	41.0	34.0	41.0
27	37.72613571966796	39.0	35.0	41.0	34.0	41.0
28	37.65248963394455	39.0	35.0	41.0	34.0	41.0
29	37.6398071021526	39.0	35.0	41.0	34.0	41.0
30	37.64522609738935	39.0	35.0	41.0	34.0	41.0
31	37.57131943283298	39.0	35.0	41.0	34.0	41.0
32	37.42900092040697	39.0	35.0	41.0	33.0	41.0
33	37.25464242523182	39.0	35.0	41.0	33.0	41.0
34	37.29692390985129	39.0	35.0	41.0	33.0	41.0
35	37.29544487132322	38.0	35.0	41.0	33.0	41.0
36	37.31370614491432	38.0	35.0	41.0	33.0	41.0
37	37.1947613776794	38.0	35.0	40.0	33.0	41.0
38	37.09693457862524	38.0	35.0	40.0	33.0	41.0
39	37.10311779134106	38.0	35.0	40.0	33.0	41.0
40	36.96855098540469	37.0	35.0	40.0	33.0	41.0
41	36.79385147406704	37.0	35.0	40.0	33.0	41.0
42	36.645774690281335	37.0	35.0	39.0	33.0	41.0
43	36.55654773295264	37.0	35.0	39.0	33.0	41.0
44	36.355787391647176	36.0	35.0	39.0	33.0	41.0
45	36.136695986065824	36.0	35.0	39.0	32.0	41.0
46	35.9109383433807	36.0	35.0	39.0	32.0	41.0
47	35.634103182653554	36.0	34.0	39.0	31.0	40.0
48	35.65420802072589	36.0	34.0	39.0	31.0	40.0
49	35.61517224786309	36.0	34.0	38.0	31.0	40.0
50	35.923765630685985	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	19.0
21	82.0
22	307.0
23	916.0
24	2556.0
25	6031.0
26	13100.0
27	27306.0
28	54815.0
29	103780.0
30	189847.0
31	343165.0
32	618345.0
33	1254603.0
34	2897623.0
35	1082910.0
36	1314573.0
37	2050324.0
38	2732682.0
39	4541406.0
40	3133422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	50.18821360130119	19.101247772466127	30.71053862623268
2	27.657339402516207	21.44699755732279	29.84121125210479	21.054451788056216
3	26.97335345134697	23.333414834462367	25.720239224470603	23.972992489720056
4	21.04615438395555	26.579678449569656	29.178361587712693	23.1958055787621
5	18.290606120950816	27.70726129890411	29.73393416358435	24.268198416560715
6	23.293714947149954	24.99421145595581	28.69976830112762	23.012305295766616
7	23.32935450713027	24.82280868994496	26.337639735158646	25.51019706776613
8	21.52897492789605	22.8183080601278	30.327709166265056	25.325007845711095
9	22.01232081043937	28.09063573630084	28.223089800104244	21.673953653155547
10	29.504238451486405	22.40172927720871	27.901435283979392	20.192596987325494
11	22.97861979900054	22.46913463868684	28.22067422447257	26.33157133784005
12	22.26881370098787	23.60725371831717	28.210707520138634	25.91322506055632
13	23.078743445050762	24.68958871191688	26.567983528523627	25.663684314508732
14	19.642734400192932	24.69984017923178	26.64451603451249	29.012909386062795
15	22.280307324064594	29.68540171415531	27.261003339778796	20.7732876220013
16	21.88581240129035	32.09538028034032	25.965145207517587	20.053662110851747
17	24.39721077605964	34.893908114645185	21.879253033314914	18.829628075980263
18	21.67073288564665	26.967235956962693	25.864398028733177	25.497633128657487
19	26.714518630765323	24.871130021991558	23.58824333448417	24.82610801275895
20	24.04279869771015	24.38112657734143	26.61846022175377	24.957614503194648
21	22.671620756982485	29.981918532649743	25.261103105016268	22.0853576053515
22	24.799433576972614	26.671082456754323	23.995046891625492	24.53443707464757
23	26.289887929073984	24.37262787527146	28.848257466583554	20.489226729071
24	23.004749257357783	26.479834656793837	24.46820022333273	26.04721586251565
25	23.37207403771741	24.582116896952822	27.851438443481808	24.19437062184796
26	23.748422842777607	31.328811934142404	26.789786714124876	18.132978508955112
27	26.925536645057633	25.39632957821909	25.25888866055137	22.4192451161719
28	24.126533239743818	24.753356948735664	24.994941491337634	26.125168320182883
29	25.436800378648638	27.87043363850318	26.83476123995935	19.85800474288883
30	25.917476286433395	26.65985082563299	28.300655327352214	19.1220175605814
31	24.02805760764122	28.920130270103435	25.25236009097024	21.799452031285107
32	23.535927421430408	26.953356568761343	26.36831139907044	23.14240461073781
33	23.124389876659848	24.390803732971825	31.632060021484655	20.852746368883675
34	22.392643206640102	30.761939457741676	25.304386223090813	21.541031112527413
35	20.495195842001735	25.83357870093265	26.540151481472652	27.131073975592955
36	22.83020334687053	24.314090578082684	27.486572602826577	25.369133472220216
37	19.165249108773384	31.094390803987558	24.33620239287203	25.404157694367026
38	24.44134671968102	25.15564557063712	26.603769744152466	23.799237965529393
39	22.994755169545414	29.052620275172707	26.368652480421783	21.583972074860096
40	18.64627629724433	26.275988649874005	28.526476855086457	26.551258197795207
41	20.689365018251465	31.814039205482203	24.447470421096593	23.04912535516974
42	21.10217250407039	32.37361192944708	25.267313184537382	21.256902381945157
43	20.56918002861103	23.90452835669184	25.721070912049466	29.805220702647667
44	19.538625389745366	27.984123289450892	24.046347445363473	28.430903875440265
45	18.42105929853439	26.377378497985237	32.280100529377656	22.921461674102712
46	18.67963976555281	27.98682041625383	31.724894851513923	21.60864496667943
47	23.16317109461895	29.644887220698614	26.739071285534898	20.45287039914754
48	24.898070595664894	28.560015635721918	23.74711407276793	22.794799695845263
49	18.54581646495844	29.63267655303636	24.104890962667408	27.716616019337792
50	18.261763558021425	30.724648095310926	28.49191898149369	22.52166936517396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	63344.0
1	69946.5
2	125736.5
3	170776.0
4	157993.5
5	106398.5
6	48871.0
7	35433.5
8	36620.5
9	35359.0
10	32916.0
11	30280.0
12	27295.5
13	24408.0
14	22794.0
15	21434.5
16	21251.0
17	22692.0
18	25107.5
19	31880.0
20	38795.0
21	45698.0
22	55970.0
23	73289.5
24	93272.5
25	106936.0
26	129642.5
27	177581.5
28	225453.5
29	258701.0
30	317037.5
31	436380.5
32	605114.5
33	675389.0
34	683851.0
35	771551.5
36	824143.0
37	819931.5
38	861883.0
39	1010922.0
40	1209977.5
41	1350373.0
42	1523033.5
43	1721636.0
44	1810311.0
45	1834840.5
46	1823811.0
47	1790363.0
48	1935997.5
49	2224112.0
50	2554233.0
51	2650573.5
52	2231240.5
53	1753759.0
54	1471599.0
55	1291523.0
56	1181241.0
57	1066841.5
58	916471.5
59	737841.5
60	582195.0
61	442544.5
62	335080.5
63	263596.5
64	246167.0
65	243620.0
66	169803.0
67	88461.5
68	49730.5
69	35285.0
70	24299.5
71	18171.0
72	13906.0
73	9939.5
74	6364.0
75	4125.0
76	2945.5
77	1917.0
78	1106.0
79	783.0
80	668.0
81	420.0
82	200.0
83	109.5
84	47.0
85	33.5
86	19.0
87	19.5
88	21.0
89	20.5
90	12.5
91	4.5
92	3.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	125971.0
25	101177.0
26	68043.0
27	135919.0
28	101959.0
29	105218.0
30	244475.0
31	388067.0
32	328241.0
33	78429.0
34	65201.0
35	75728.0
36	87473.0
37	138072.0
38	53281.0
39	58958.0
40	64295.0
41	90680.0
42	106210.0
43	215763.0
44	761707.0
45	1701450.0
46	2095544.0
47	2661570.0
48	3307358.0
49	4173251.0
50	3033776.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.56748634199508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.86682918517818	5.900156184605222
2	12.850381927830327	2.2019094331221343
3	5.2061541476558375	1.338109636630873
4	2.7256719622721794	0.934086292381033
5	1.597912147461448	0.6845045249541991
6	1.0548684304683482	0.5422542522383617
7	0.7376592440044809	0.44239198486383646
8	0.6022782886966821	0.41280088099911955
9	0.47049217872941007	0.36278417837517557
>10	3.7780047746197263	6.439688042401298
>50	0.4969257301856972	3.035906309159051
>100	1.260818314995283	29.59704624329832
>500	0.25713114280032845	14.279017118065024
>1k	0.08157263840561986	13.004236844144025
>5k	0.00709327290483651	4.163879978564858
>10k+	0.006206613791731946	16.661228096197497
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	240546	1.1810102762122359	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	131338	0.644831041285919	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	82758	0.4063174961910497	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	69119	0.3393540083040813	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	63001	0.30931642351835853	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	52166	0.2561197528492991	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG	49917	0.24507782277687504	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA	49621	0.24362454963261648	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	44828	0.2200923260500782	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	43767	0.2148831273809622	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	41605	0.20426834177999253	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	39094	0.19194006858663687	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	33303	0.1635079578487944	No Hit
AGGAAAGGCACTTGCTGCTGAGGCAGG	30238	0.14845970721652238	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	27430	0.13467325117233975	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	26484	0.13002866875859445	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA	26168	0.12847720148296704	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	25709	0.12622364616805257	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	25223	0.1238375287757902	No Hit
CGTTTGGTATTGGGTTATGGCAGGGGG	22126	0.10863216753332808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.025604119754420406	0.0	0.0	0.0	0.0
2	0.03879159159725323	0.0	0.0	0.0	0.0
3	0.05527838625407849	0.0	0.0	0.0	0.0
4	0.09261179500050472	0.0	0.0	0.0	0.0
5	0.11294288990041937	0.0	0.0	0.0	0.0
6	0.17501631004522036	0.0	0.0	0.0	0.0
7	0.20277088127661796	0.0	0.0	0.0	0.0
8	0.24340361283703663	0.0	0.0	0.0	0.0
9	0.28359938051286404	0.0	0.0	0.0	0.0
10	0.31413775536856775	0.0	0.0	0.0	0.0
11	0.34629633339185706	0.0	0.0	0.0	0.0
12	0.3830012996975228	0.0	0.0	0.0	0.0
13	0.4149733088712113	0.0	0.0	0.0	0.0
14	0.44698950540401583	0.0	0.0	0.0	0.0
15	0.4805326206796055	0.0	0.0	0.0	0.0
16	0.5155191896863169	0.0	0.0	0.0	0.0
17	0.549234144691802	0.0	0.0	0.0	0.0
18	0.5797872486672111	0.0	0.0	0.0	0.0
19	0.6109196980176962	0.0	0.0	0.0	0.0
20	0.6426020345038467	0.0	0.0	0.0	0.0
21	0.6742941904031341	0.0	0.0	0.0	0.0
22	0.7012484794638758	0.0	0.0	0.0	0.0
23	0.7293172719156537	0.0	0.0	0.0	0.0
24	0.7556676670684771	0.0	0.0	0.0	0.0
25	0.7823961096270705	0.0	0.0	0.0	0.0
26	0.8133910871936392	0.0	0.0	0.0	0.0
27	0.8385827915963106	0.0	0.0	0.0	0.0
28	0.8681539542580314	0.0	0.0	0.0	0.0
29	0.8985057602641343	0.0	0.0	0.0	0.0
30	0.9273650154734312	0.0	0.0	0.0	0.0
31	0.9549722955077756	0.0	0.0	0.0	0.0
32	0.9835958848017873	0.0	0.0	0.0	0.0
33	1.0135745531086888	0.0	0.0	0.0	0.0
34	1.0443731424125198	0.0	0.0	0.0	0.0
35	1.0716612915199155	0.0	0.0	0.0	0.0
36	1.0942753999741552	0.0	0.0	0.0	0.0
37	1.1107130975652961	0.0	0.0	0.0	0.0
38	1.1242147906285092	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	25670	0.0	148.14687	44
AGCGGTA	315	0.0	135.3642	44
CTATACG	795	0.0	117.32629	44
ACTTCTA	20625	0.0	113.253685	44
GCGTCAC	95	1.8189894E-12	112.20979	44
GTACGAC	490	0.0	108.774796	44
GAACACA	12855	0.0	104.69204	44
TAATCGT	4250	0.0	102.58459	43
ACGGATA	45	5.456968E-12	100.01078	43
CCTTAGC	53860	0.0	97.0955	43
TCCGGGC	3120	0.0	97.005646	43
GAACGGT	100	4.080357E-8	79.94948	44
CAATGAA	27520	0.0	78.006226	43
ACGACGC	130	0.0	77.02517	42
CCAGAAT	10525	0.0	75.07529	44
GAATCAC	3040	0.0	72.76104	44
ATGACCG	390	0.0	71.749535	44
CCCATAC	6570	0.0	68.75411	44
TTAATCG	4595	0.0	68.55994	42
ACCTTAG	55410	0.0	66.42569	42
>>END_MODULE