FastQCFastQC Report
Tue 24 May 2016
ERR841077.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841077.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5461676
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC278610.5101181395600911No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA258150.47265711111387787No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA159910.29278558449823827No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG148920.27266355602199766No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT138380.2533654504587969No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC131990.24166574509363062No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG108910.19940765435371854No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG99120.18148275364558425No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC73790.1351050483404728No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT72350.13246849501874516No Hit
ATCTACCTGAGGAAAGGCACTTGCTGCTGAGGCAGG71740.13135162173662443No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA60920.11154085302753222No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA57950.106102961801469No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATG100.0011910753263.2018744
AACGCAT1200.0252.2351444
TGAACCG700.0225.6016244
ACTGCGT950.0221.643744
AATGCGA850.0216.754544
GCCTATT850.0216.754544
ATATACT1100.0215.3469844
AACGCCC550.0215.3469844
CTATCAT81150.0185.5224844
CTCGCTA1000.0184.2413344
ACCGAAC308.242205E-7175.4679344
AATCGCA3350.0172.8490144
ATTAGTG850.0170.307144
ACTTCTA52200.0135.6346944
GTCACGC702.5465852E-11131.6009544
AACGGTA301.8096133E-4131.6009444
AGCGCCC200.009474494131.6009444
AAGCCCG1150.0125.8791644
TATCACG1050.0125.33423644
TGGTACA1800.0116.97861544
Produced by FastQC (version 0.11.5)