FastQCFastQC Report
Tue 24 May 2016
ERR841076.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841076.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001677
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT74590.7446512199042206No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC50570.5048533609137477No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC41330.4126080562896023No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG36630.3656867433314332No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG33860.338033118460342No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA33780.3372344578142455No Hit
AGACGGAAGTGAGCGACACACTCTGCGTCCTCGCCTCACCAGGAAACTA33160.33104483780699767No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA28800.2875178325947386No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA24440.2439908273824796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA23630.23590438834075253No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA22300.22262665509939833No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA22250.22212749219558797No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA21070.21034724766566468No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC20100.2006634873317447No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA19990.19956532894336199No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG19090.19058039667477641No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT18880.1884839124787731No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT18810.18778508441343866No Hit
AGACGGAAGTGAGCGACACACTCTGCGTCCTCGCCTCACCAGGAAAC18410.1837917811829562No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA18030.17999814311399784No Hit
TCCCAATGATGAGTTGCCATGCTAATACTGAGCCACCAGGTAGGGCAGTG17860.1783009892410428No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA17560.1753060118181809No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.17440751859132236No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG17360.17330936020293966No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17230.17201153665303287No Hit
AGGAAAGGCACTTGCTGCTGAGGCAGG17070.17041421536083987No Hit
AGAAAAAGGTGATGTGAAGATGGAGGTAGAGACTGAGGGATGTGTCTAC16750.16721957277645388No Hit
CACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAG16550.16522292116121265No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT16410.1638252650305438No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG16120.16093012018844396No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA16080.16053078986539573No Hit
ATGAAGAAGGAGAAGGAGAAGAAGATGATGATGATGATGAAGAGGAGGA15900.15873380341167861No Hit
ACCATTTGGTGAGCTGAGGGTGAAGCTACAAAGGTTGAAGCGTTCTTCA15520.15494016534272026No Hit
CAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAAC14940.14914987565852064No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG14940.14914987565852064No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT14860.14835121501242415No Hit
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGG14810.14785205210861385No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG14790.14765238694708974No Hit
AAAAAAAAAAAAAAAAAAAAAAAA14440.14415824662041757No Hit
CCCATCACCTGAGATGGGGAGTTCCTGACCAACATGGAGAAACTCCAT14340.14315992081279694No Hit
TGCGTTGCGTTCCAAGGCATCTGTGAGCCCGCGGAGTATACACCATGAG13880.13856762209774207No Hit
CATATCTGTGAGGCCAGCAAATATTTTCTTAAACTCATGGGGAGACAGCA13780.13756929629012146No Hit
CTCTCGTTCTCTCCTGCTCCTTTGTGTGGAGATGTGCCAGCTTCCTCTT13640.1361716401594526No Hit
AGGGAGGAAGGGAGGGGAAGGAAAGGAAGGAAGGAAAGGAAATAAGGAAA13450.1342748211249734No Hit
CTGAAAAGGCACTTTTCAGAGCCCTCAAATCTAGACGGGATACCCCTAA13410.13387549080192518No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA13410.13387549080192518No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13290.13267749983278043No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT13190.1316791740251598No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA13130.13108017854058743No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA13040.13018168531372887No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG12890.12868419660229793No Hit
AAGGGCGTGCAAAAACTAACATTGAAATTTTGAAACAGCAGCAGAGTGAG12830.1280852011177256No Hit
ACGCTGAGCCGCGGACACCATGCAGTCGGATGATGTTATCTGGGATACA12820.1279853685369635No Hit
CCGCAGGAGGCGTGAGGGGATAAAAACATTCAGATGGCAGATCACAGT12790.12768587079467733No Hit
TGGCATCATCTCCATGTGCCCTGGGGGCAAATTTAGCTGAGTTTTGAATG12770.1274862056331532No Hit
CTAAGGTGACACTCCACCAACCAGAGTGAGAGGGCAGATAGGCAGGATT12730.12708687531010496No Hit
TTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACC12650.12628821466400844No Hit
ATCCTGTTGATCTGTGACCTTACCCCCAACCCTGTGCTCTCTGAAAC12460.12439139562952929No Hit
CATCCTTTTGCTATAAAGGATATTATTAAGACAAATTGGTGAAATGTCAG12140.1211967530451433No Hit
AGGCTGTGGCTACTGGAACGCTGAGTTCTCTCAGACACATTGAAAAAG12080.12059775756057091No Hit
CATTCTGGACGCACATTTGATGCCCAGATAGTGATTATAGAGCACAAATC12020.11999876207599855No Hit
ACTAGCCGGGTTGTATTTGGAAACGCGGAGTGAGTTTTTCCGTGCTGTGT11990.11969926433371236No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11910.11890060368761586No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT11820.11800211046075731No Hit
TCACGGGGTATTGTGCACAACTGTTTGCCGTAGCAACACAGTGTCTGTG11750.11730328239542287No Hit
CAGAAGTCCTTATAGTCCAGGGCCTGTTTCCCTGTAGCAGCTCCTTAT11730.11710361723389875No Hit
CAAATGGTGTAAATTTGATGACGACGTGGTGTCAAGGTGTACTAAAGA11730.11710361723389875No Hit
TGGTTGAGTCTGAAGCGCTGGGAGGCGGACATTAAAGTGAAGTGGTTG11720.1170037846531367No Hit
AGACGGAAGTGAGCGACACACTCTGCGTCCTCGCCTCACCAGGAA11690.11670428691085051No Hit
CCGCGCCAGAACTGTACTCTCCGAGAGGTCGTTTTCCCGTCCCCGAGAG11610.11590562626475401No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT11520.11500713303789546No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC11380.11360947690722657No Hit
AGATTCTGGTAACCACTATCAGGAGATCTGTATTAGGTCAGTTCACTCA11340.11321014658417833No Hit
TGCTTGTCACGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACC11320.11301048142265421No Hit
AACCATATCTGAAAAGTGAAGGCTCACCTAATAGCATCAGGTAAGAAAAA11210.11191232303427152No Hit
AGAGACTAGCCTAGTCTCCCAGCCTACATCTTTCTCCCATGCTGGAT11180.11161282529198534No Hit
TAAGAGAGAGAGAAAGAGAGACAGCACTGTGCGTCATGTACCAGCGCCG11140.11121349496893708No Hit
CTTTGTCTCTAGCTGCTGCGGGAGCTCCAGGTCTAGTCTTTACTGCTCT11100.11081416464588884No Hit
TGAGCAGAACCGTAGTCAGAAGGACATTTCTGGTAAGGAATGAGGAATG11080.11061449948436472No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA11030.11011533658055442No Hit
TCTCAGTGAGTCTTCACATGCCTCCAACTGCCTGGACAACCACACGTGA10970.10951634109598203No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC10940.10921684335369584No Hit
CATGAAATCATCACATTAGAACAATATCAGCTATGTGCATTAGCTATCAA10920.10901717819217173No Hit
ATTGCTGAGTTTAAGCGCAAGCATAAGAAGGACATCAGTGAGAACAAGAG10890.10871768044988554No Hit
AGAGCAGAGTACGAGTCTGAGGCGGAGGGAG10880.10861784786912348No Hit
ATTGTGGGATTGGATGAGTCTCAAGATGGACAACCGGGATGTTGCAGGA10870.10851801528836143No Hit
CTCTAACAAAACTAGGCTGTCCCGAACCCCTGGTAATAGAATTGTTTACC10860.10841818270759937No Hit
TGAGCATCTGCCTTCCTTTTTTGAATTTTAGAGCTTACATTCTTAGTGAG10840.10821851754607523No Hit
TACTTCCGGTGCAGTGAAGGCTCGGGGCTGAAGCGGGG10840.10821851754607523No Hit
AAGCAGTGGGGGTTTATTTGCCCTCCCCAGGATGGACTAGAGGATGGGCA10830.10811868496531317No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT10820.10801885238455111No Hit
AGAACGGAGCGGACGCCATGGCGACCAACATCGAGCAGATTTTTAGGT10780.10761952206150287No Hit
TACCCAGTGGCAAGGGCTGCGGGAATTGAGGGTGCTGGCGTAAAACCAG10760.10741985689997874No Hit
AGTGGAGTGAGGGGTAACAAGATGGCGACCGAGACGGTGGAGCTCCATA10750.10732002431921668No Hit
AAGCAACAGCGACGCAAGATGGCAGCCACCACGGGCTCGGGAGTAAAAG10680.10662119625388224No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG10660.10642153109235812No Hit
CTCTTGAGCTGCTTGCTTGGCCCACTCTCACTCTGTGGAGTGTACATTT10640.10622186593083399No Hit
ATCACAGGTGGCTCTGCTGTGAATGTGCCTGCCTGTCATTTGAATTGAGC10600.10582253560778573No Hit
TGAAAGTATTTGATGAATTGTTGCATCAGAGATATGAGAGATTATTTTT10520.10502387496168925No Hit
CTGGAGAGATGTGAAGAAGACTGGGTTTGTCTTTGGCACCACGCTGATCA10520.10502387496168925No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA10510.10492404238092719No Hit
AGTCACTCCGGCGCAGTGTTGGGACTGTCTGGGTATCGGAAAGCAAGC10460.10442487947711689No Hit
TCTGCTGCTATTCTCCGAGCTTCGCAATGCCGCCTAAGGACGACAAGA10440.10422521431559274No Hit
CTTCTGGTGTACTGGGTGGGAGGTGGAACTAGTCGGACAAAGCCCTCGC10430.10412538173483069No Hit
AGCTAGAGGCAAAATGATATCTGCTGGGCTTCCAGGAGAGATGGGATCC10410.10392571657330657No Hit
CAGGCCATCAAGGGTATGCATATACGAAAAGCCACGAAGTATCTGAAAGA10390.10372605141178244No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGC10390.10372605141178244No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA10330.10312705592721008No Hit
CCATTTTGGATGGTGAACCCTGAAGTCGGTGTCTGCTGCGTTCACAGCA10310.10292739076568594No Hit
TTTTCTTGTAAATACAGTTTTGTACAATGTTATCTCTGTGGGAGGAAG10280.10262789302339975No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT10230.10212873011958945No Hit
ACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAATAGTACTT10230.10212873011958945No Hit
AGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGG10220.10202889753882739No Hit
ACAGAGTTTTAGTTTCTAGCTGAGGTCTCCACTGAAAACAACCGAGTTC10220.10202889753882739No Hit
ACTGAATCTGATACAGCTTCAGTTAATGAAAATGGGCTAGTCACAGTTG10200.10182923237730325No Hit
CCAGACGACAAGCACACACTATGGCGCTGAAACGGATTAATAAGGAACTT10180.10162956721577913No Hit
TGTTGGGGTGGGCAGTAGTCTATAGCTGTGTGTGGAGTTGGCTCATAAT10180.10162956721577913No Hit
CAGTTCCCTGCCCAACAGGCATTCCCCCTTTTCCTTGGCAACAGATGCTA10160.101429902054255No Hit
CTCTCTCGAGTCACTCCGGCGCAGTGTTGGGACTGTCTGGGTATCGGAA10050.10033174366587234No Hit
CTGTCACTGCCTAGCTCGAAACCTTCACCGACATCCCTTGTCTGCTAAC10030.1001320785043482No Hit
AAGCTGGGTCTTGTTATTCCTTGGGTGTTGGTGGAATAAGCAGTGGAATT10030.1001320785043482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAA1000.0243.5848544
TACGGGC500.0243.5848544
AATATCT750.0243.5848544
GCTAGCC251.6643753E-9243.5848544
TCGAGAA450.0243.5848544
AGTCCGT1250.0243.5848444
AGTCTTA1050.0243.5848444
TCCACAA550.0243.5848444
TAGTGTC100.0015008479243.5848444
GACCTCG1050.0243.5848444
TATCCAT400.0243.5848444
ACAGTTC151.5524583E-5243.5848244
TCAATTG301.8189894E-11243.5848244
GCCGAAC700.0243.5848244
GATGCTA950.0243.5848244
CTACATG700.0243.5848244
ACTTTAC600.0243.5848244
GCTCCCC301.8189894E-11243.5848244
TGCGTAC301.8189894E-11243.5848244
ACAAATC1600.0235.9728144
Produced by FastQC (version 0.11.5)