FastQCFastQC Report
Tue 24 May 2016
ERR841075.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841075.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202573
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC480992.1837641703589394No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA284191.290263705221121No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT283441.286858596741175No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC153360.6962765819793487No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT145290.6596376147351303No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA135480.6150987958174372No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA112260.5096766372783104No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA85980.39036163614100416No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT83260.3780124427204002No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG82710.3755153631684398No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG76820.34877391123926427No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC75990.34500559118812407No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG64090.29097786997298164No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA57880.26278357175902906No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAG50740.23036693902994365No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT48030.21806314705573893No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC46110.20934606934707728No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC44550.20226344370878968No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC43850.19908534246084014No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT38470.1746593642980278No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC38080.17288870788845592No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC37570.17057323412209266No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG34530.15677119441671175No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC32930.14950696299282704No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA32700.1484627297256436No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC32460.1473730950120609No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA29760.13511470448425547No Hit
TACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGAT26540.1204954387436875No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCA26280.11931500113730623No Hit
ATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTC26110.11854317654851848No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT25990.11799835919172715No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG25770.11699952737094298No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT25310.11491106083657612No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT24450.1110065364462381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC153.869358E-5193.7979144
ATTGTGT1350.0193.7979144
GTAGACT600.0193.7979144
CGAGCTA1000.0193.7979144
CTCGACG850.0193.7979144
TGTACAG100.0029772134193.7979144
TCGACAA153.869358E-5193.7979144
TACCGCC256.553819E-9193.7979144
TGGACTC1250.0193.797944
GCTTGCG1250.0186.0459944
AGGTCTC1250.0186.0459944
GCGATGG1100.0184.988944
CGGAAAA1050.0184.5694444
AGACCAC1800.0183.0313444
AGGGATA900.0183.0313444
AACTTCT1600.0181.6855544
GCTTAAT600.0177.6480944
TCCCGTT600.0177.6480944
AGCACGT1050.0175.3409744
CCGGGCT1000.0174.4181244
Produced by FastQC (version 0.11.5)