##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841075.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2202573
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.657828821110584	39.0	38.0	39.0	35.0	39.0
2	38.01853423246358	39.0	38.0	39.0	35.0	39.0
3	38.223401449123365	39.0	38.0	39.0	35.0	39.0
4	38.722777406242606	39.0	39.0	41.0	35.0	41.0
5	39.44524789870756	41.0	39.0	41.0	36.0	41.0
6	39.46349701008775	41.0	39.0	41.0	36.0	41.0
7	39.46473873964677	41.0	39.0	41.0	36.0	41.0
8	39.43076983146529	41.0	39.0	41.0	36.0	41.0
9	39.37545270917241	41.0	39.0	41.0	36.0	41.0
10	39.36401744686782	41.0	39.0	41.0	36.0	41.0
11	39.285600068646986	40.0	39.0	41.0	36.0	41.0
12	39.24334675854104	40.0	39.0	41.0	35.0	41.0
13	39.1429178510769	40.0	39.0	41.0	35.0	41.0
14	39.186697557810795	40.0	39.0	41.0	35.0	41.0
15	39.14362157349609	40.0	38.0	41.0	35.0	41.0
16	39.24296220829003	40.0	39.0	41.0	35.0	41.0
17	39.30456243675011	40.0	39.0	41.0	35.0	41.0
18	39.27896646331359	41.0	39.0	41.0	35.0	41.0
19	39.24343302128919	41.0	39.0	41.0	35.0	41.0
20	39.212298071391956	41.0	39.0	41.0	35.0	41.0
21	39.31108299248197	41.0	39.0	41.0	35.0	41.0
22	39.28364099623486	41.0	39.0	41.0	35.0	41.0
23	39.32228670740992	41.0	39.0	41.0	35.0	41.0
24	39.23040416821599	41.0	39.0	41.0	35.0	41.0
25	39.25818279324644	41.0	39.0	41.0	35.0	41.0
26	39.162229443260394	41.0	39.0	41.0	35.0	41.0
27	39.17701335017015	41.0	39.0	41.0	35.0	41.0
28	39.111059278832386	40.0	39.0	41.0	35.0	41.0
29	39.121939011314055	40.0	39.0	41.0	35.0	41.0
30	39.11226876343427	40.0	38.0	41.0	35.0	41.0
31	39.03096854937337	40.0	38.0	41.0	35.0	41.0
32	38.85415461327649	40.0	38.0	41.0	35.0	41.0
33	38.7899827620159	40.0	38.0	41.0	35.0	41.0
34	38.81218852633326	40.0	38.0	41.0	35.0	41.0
35	38.87081115298135	40.0	38.0	41.0	35.0	41.0
36	38.87405146767739	40.0	38.0	41.0	35.0	41.0
37	38.81959547870026	40.0	38.0	41.0	35.0	41.0
38	38.795466284528025	40.0	38.0	41.0	35.0	41.0
39	38.74520405822463	40.0	38.0	41.0	35.0	41.0
40	38.72334028974386	40.0	37.0	41.0	35.0	41.0
41	38.67339807472117	40.0	37.0	41.0	35.0	41.0
42	38.647673883303185	40.0	37.0	41.0	35.0	41.0
43	38.56530559235828	40.0	37.0	41.0	35.0	41.0
44	38.35063405912128	40.0	37.0	41.0	34.0	41.0
45	38.221676309682564	40.0	36.0	41.0	34.0	41.0
46	37.96773617742871	40.0	36.0	41.0	34.0	41.0
47	37.68407837522537	39.0	36.0	41.0	33.0	41.0
48	37.627378731941185	39.0	36.0	41.0	33.0	41.0
49	37.78563298283694	39.0	36.0	41.0	33.0	41.0
50	37.2390251312991	38.0	36.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	8.0
21	16.0
22	40.0
23	81.0
24	141.0
25	281.0
26	427.0
27	914.0
28	1933.0
29	3946.0
30	7801.0
31	14409.0
32	24767.0
33	44021.0
34	81161.0
35	75096.0
36	108683.0
37	174270.0
38	269751.0
39	459114.0
40	935710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	44.39153662557382	18.748663494921622	36.859799879504564
2	24.21685910069723	20.98781742988768	34.84166018561019	19.953663283804897
3	21.85866257327226	24.487406319790537	23.01272193929554	30.641209167641666
4	20.70428539712418	26.3671624050599	27.56812146521364	25.36043073260228
5	14.671114192355942	31.23269921133148	28.132143633831884	25.9640429624807
6	17.948962418044715	28.237066376460625	28.36614268857377	25.44782851692089
7	19.513133049392685	26.054437242261663	21.48618910701257	32.94624060133307
8	19.846606673195392	25.058738121279067	32.631018358982885	22.463636846542656
9	19.910713515511176	27.11764831403999	30.196184190035925	22.77545398041291
10	27.70037587857474	21.066407333604833	29.88990603262639	21.34331075519404
11	24.1116639493901	23.271056169307442	24.86451073358295	27.75276914771951
12	19.273231806618895	26.284985787077204	25.814899211059068	28.626883195244833
13	21.402014825388306	24.737023472093775	26.130257657748462	27.73070404476946
14	18.53441406936342	25.114309491671786	25.166521155030956	31.184755283933836
15	19.224788463310862	33.33832749243725	25.51361521275345	21.923268831498433
16	19.790399682553087	33.8647118619905	23.322496008077824	23.02239244737859
17	20.743103633795563	38.25003756969689	21.69281108957569	19.31404770693185
18	18.48469948555621	28.374314948925644	21.65821518741944	31.482770378098706
19	20.16505241823994	25.718012524443	22.490741510043026	31.626193547274028
20	21.3707332288192	21.79768843075803	30.639347708339294	26.19223063208348
21	18.60841842699425	33.85772003924501	23.834079506104906	23.699782027655836
22	21.38653293216615	29.04439489633261	24.24201150200243	25.327060669498806
23	26.014211560751903	23.805476594873358	28.897294209998943	21.283017634375796
24	20.414941979221574	28.267212936869747	23.452934363582955	27.86491072032573
25	20.74314244208412	25.13435591787456	24.2537641281422	29.86873751189912
26	19.44738046537632	33.25761596724405	28.5057384110407	18.789265156338928
27	21.187306960443415	29.356491283695586	21.884483021682744	27.57171873417826
28	20.42970545245895	27.535005208299758	24.247974012840693	27.787315326400602
29	19.983916290763883	30.967194779264958	24.634557790431117	24.414331139540042
30	23.41289950142371	30.23458701325701	26.39697884299116	19.955534642328125
31	24.66400853323548	30.098423326548367	21.513516650620826	23.724051489595322
32	19.539909409257003	30.8058484317905	25.37211670219245	24.28212545676005
33	21.590696338892755	24.76265032307882	30.55066483131124	23.095988506717188
34	21.60306654812358	29.918893364263543	25.711878980938213	22.76616110667467
35	18.629738648060467	24.96145535971825	25.18767176706982	31.22113422515146
36	16.490939948497047	28.45954627230717	28.725929032536225	26.323584746659563
37	18.95446787397912	33.95518333175162	22.748754341304338	24.34159445296492
38	25.57175123722652	24.872870098869484	24.703643386430457	24.851735277473537
39	18.19457856307297	32.05946923209935	25.996063064299484	23.74988914052819
40	16.722819925717662	29.7327157883018	28.625538194073417	24.91892609190712
41	14.893246556923945	32.73233435692245	25.401307633014614	26.973111453138994
42	20.41770965507119	31.783974788943564	23.8702538287714	23.928061727213844
43	20.240938140551947	23.577546804337935	24.908284035582977	31.27323101952714
44	19.65286120289638	27.131126931194487	23.100629106526505	30.115382759382626
45	18.04400785442021	26.392532000462026	32.59484632427835	22.968613820839415
46	19.582562409105318	27.85865569122296	27.5763744191608	24.982407480510922
47	18.60191960971471	29.48801569625623	29.284388588397498	22.625676105631563
48	22.067245446038278	25.83115409760412	23.587662961965925	28.513937494391673
49	15.916883740584801	30.6398511224085	24.617653671266318	28.82561146574038
50	18.211944962889014	31.209253873866054	27.859933156821043	22.71886800642389
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20109.0
1	17981.0
2	13739.5
3	11163.5
4	9858.0
5	6939.0
6	3851.5
7	3483.5
8	4702.0
9	5517.5
10	5537.5
11	4797.5
12	3780.0
13	3164.5
14	3328.0
15	3556.5
16	3325.0
17	3046.0
18	3499.0
19	4801.5
20	6186.5
21	7900.0
22	9478.0
23	10853.0
24	12310.5
25	15374.0
26	17976.5
27	19486.0
28	21793.5
29	25810.5
30	34547.0
31	51565.0
32	78732.5
33	92736.5
34	95432.0
35	103779.5
36	107685.0
37	113769.0
38	116741.0
39	118614.5
40	127232.5
41	127713.0
42	128573.5
43	141212.5
44	150875.0
45	149211.5
46	150255.0
47	158504.0
48	187705.0
49	233456.5
50	284596.5
51	307740.0
52	257362.5
53	186496.0
54	154832.0
55	140964.0
56	123877.5
57	110744.5
58	100110.0
59	87459.0
60	70787.0
61	53434.0
62	41106.5
63	31930.5
64	22128.0
65	15675.0
66	11369.0
67	7423.5
68	4976.0
69	3715.5
70	2935.0
71	2239.0
72	1399.5
73	695.0
74	443.5
75	280.5
76	158.5
77	91.5
78	87.5
79	75.0
80	61.0
81	39.0
82	83.5
83	145.0
84	141.5
85	140.5
86	71.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	5982.0
25	7309.0
26	6763.0
27	10985.0
28	7854.0
29	8744.0
30	18349.0
31	34827.0
32	40034.0
33	6777.0
34	6026.0
35	7875.0
36	7385.0
37	8569.0
38	6773.0
39	6268.0
40	7668.0
41	8118.0
42	9699.0
43	17903.0
44	64005.0
45	156758.0
46	239262.0
47	260496.0
48	398587.0
49	388777.0
50	460780.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.666017095957383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.56000026485471	4.570221338643641
2	11.954765270330562	1.5938133934036223
3	5.0907074538867105	1.0180422875437947
4	2.762732737515671	0.7366569463936242
5	1.6650403173319162	0.5549593610396429
6	1.1185882806425704	0.447391716126057
7	0.8402871552198241	0.39209579795461197
8	0.5811365361634074	0.3099092868120584
9	0.4751304892796733	0.2850505167914008
>10	3.786702189060194	5.2626140207012115
>50	0.7573250349205559	3.596421888462916
>100	1.6523455307357584	28.709302293722256
>500	0.5862488562332677	27.342448731189013
>1k	0.15855840951504752	15.109978551342573
>5k	0.005563452965440263	2.677011791609539
>10k+	0.004868021344760231	7.394082078264032
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	48099	2.1837641703589394	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	28419	1.290263705221121	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	28344	1.286858596741175	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	15336	0.6962765819793487	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	14529	0.6596376147351303	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	13548	0.6150987958174372	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	11226	0.5096766372783104	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	8598	0.39036163614100416	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	8326	0.3780124427204002	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	8271	0.3755153631684398	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	7682	0.34877391123926427	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	7599	0.34500559118812407	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	6409	0.29097786997298164	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	5788	0.26278357175902906	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAG	5074	0.23036693902994365	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	4803	0.21806314705573893	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	4611	0.20934606934707728	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	4455	0.20226344370878968	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	4385	0.19908534246084014	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	3847	0.1746593642980278	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	3808	0.17288870788845592	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	3757	0.17057323412209266	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	3453	0.15677119441671175	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	3293	0.14950696299282704	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	3270	0.1484627297256436	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	3246	0.1473730950120609	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA	2976	0.13511470448425547	No Hit
TACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGAT	2654	0.1204954387436875	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCA	2628	0.11931500113730623	No Hit
ATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTC	2611	0.11854317654851848	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	2599	0.11799835919172715	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG	2577	0.11699952737094298	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT	2531	0.11491106083657612	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT	2445	0.1110065364462381	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.048534146200829666	0.0	0.0	0.0	0.0
2	0.07336873738123549	0.0	0.0	0.0	0.0
3	0.09970157629281753	0.0	0.0	0.0	0.0
4	0.1255804007404068	0.0	0.0	0.0	0.0
5	0.15141382374159676	0.0	0.0	0.0	0.0
6	0.2253727799260229	0.0	0.0	0.0	0.0
7	0.26069510522466227	0.0	0.0	0.0	0.0
8	0.30568793860634813	0.0	0.0	0.0	0.0
9	0.3483198967752715	0.0	0.0	0.0	0.0
10	0.3810089381827526	0.0	0.0	0.0	0.0
11	0.42050819655012567	0.0	0.0	0.0	0.0
12	0.45737417102634054	0.0	0.0	0.0	0.0
13	0.49809926844649416	0.0	0.0	0.0	0.0
14	0.5355100602795004	0.0	0.0	0.0	0.0
15	0.5742374940580857	0.0	0.0	0.0	0.0
16	0.6091512063391316	0.0	0.0	0.0	0.0
17	0.6666748389270185	0.0	0.0	0.0	0.0
18	0.7012707410832695	0.0	0.0	0.0	0.0
19	0.7411332110218367	0.0	0.0	0.0	0.0
20	0.7879875037058931	0.0	0.0	0.0	0.0
21	0.8204949393277771	0.0	0.0	0.0	0.0
22	0.8523667547000713	0.0	0.0	0.0	0.0
23	0.8857822192499408	0.0	0.0	0.0	0.0
24	0.9196970997102025	0.0	0.0	0.0	0.0
25	0.95388438884886	0.0	0.0	0.0	0.0
26	0.9922940125026503	0.0	0.0	0.0	0.0
27	1.0184452456286353	0.0	0.0	0.0	0.0
28	1.0551750157656523	0.0	0.0	0.0	0.0
29	1.0956731059538094	0.0	0.0	0.0	0.0
30	1.1292701762892763	0.0	0.0	0.0	0.0
31	1.1575552773960274	0.0	0.0	0.0	0.0
32	1.1896541000003178	0.0	0.0	0.0	0.0
33	1.22039087921263	0.0	0.0	0.0	0.0
34	1.2546235697976866	0.0	0.0	0.0	0.0
35	1.281001810155668	0.0	0.0	0.0	0.0
36	1.3052915839792825	0.0	0.0	0.0	0.0
37	1.3231797538605985	0.0	0.0	0.0	0.0
38	1.3386162456363535	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	15	3.869358E-5	193.79791	44
ATTGTGT	135	0.0	193.79791	44
GTAGACT	60	0.0	193.79791	44
CGAGCTA	100	0.0	193.79791	44
CTCGACG	85	0.0	193.79791	44
TGTACAG	10	0.0029772134	193.79791	44
TCGACAA	15	3.869358E-5	193.79791	44
TACCGCC	25	6.553819E-9	193.79791	44
TGGACTC	125	0.0	193.7979	44
GCTTGCG	125	0.0	186.04599	44
AGGTCTC	125	0.0	186.04599	44
GCGATGG	110	0.0	184.9889	44
CGGAAAA	105	0.0	184.56944	44
AGACCAC	180	0.0	183.03134	44
AGGGATA	90	0.0	183.03134	44
AACTTCT	160	0.0	181.68555	44
GCTTAAT	60	0.0	177.64809	44
TCCCGTT	60	0.0	177.64809	44
AGCACGT	105	0.0	175.34097	44
CCGGGCT	100	0.0	174.41812	44
>>END_MODULE