FastQCFastQC Report
Tue 24 May 2016
ERR841074.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841074.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415988
Sequences flagged as poor quality0
Sequence length24-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT115212.769551044741675No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT40770.9800763483562026No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA35890.8627652720751561No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA35140.8447359058434378No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG19720.47405213611931113No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA18670.44881102339490564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA17840.4288585247651374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA17560.4221275613719626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA15770.3790974739655951No Hit
CTATAATTTTCAACACCTGCTTAGCCTTTTCCAGTGGACACTTCTGTGGC15360.3692414204255892No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA15070.36227006548265817No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG14670.35265440349240845No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14150.3401540429050838No Hit
ATTTTGATGTATATGAAACTTTTGTTAAATATGTTGTATACTTCGCTGGC13700.3293364231660529No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13480.3240478090714155No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC13200.3173168456782407No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACC12590.3026529611431099No Hit
AAAAAAAAAAAAAAAAAAAAAAAA12160.29231612450359146No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12080.2903929921055415No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.28943142590651655No Hit
TTAAAAGGCTTTTGGAACTCTTCTAAACTGGGAGGTCTGATTGAGAATCA11960.28750829350846663No Hit
ACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTA11930.28678711885919783No Hit
CACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAA11680.28077733011529177No Hit
CTTTCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGAC11510.2766906737694357No Hit
AGAGAAAAGGCCACATAACAACAGAGACAGAGATTGGAATTACGCAGCTA11160.26827696952796715No Hit
AATGAATTCTCTCACCTGTGTGATGATTCTCAAAAGCAAGAGAAGGAA11150.2680365779782109No Hit
ACGCGTCCCTTTGGGTTTAGCACGATGAGCTCAATCGGCACTGGGTATGA11020.26491148783137974No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10930.2627479638835735No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG10810.25986326528649867No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT10600.25481504274161754No Hit
CTGGAGAGAACAGGATGCCTCCGTAATTTTCCGGGTCCTTTGTGTTTC10560.25385347654259255No Hit
AGACTGGCCTACCTTCCCAGCCTACATATTTCTCCCATGCTGGATGCTT10490.2521707356942989No Hit
TTGTCTGTGGCCGGAGGCTGATCAGTGTTCTAGAACAGATCAGACATTTT10400.2500072117464927No Hit
CGGTCCTCAAGCCTTATTGAACATAAGAGAAGTCATGCTGGAGAGAAACC10280.24712251314941777No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA10280.24712251314941777No Hit
TTATTGTAATGATGTTGATCGGGAGTTGAGAAAATGCCTGAAGAATGAGC9940.23894920045770554No Hit
TTTTTCAGCTCACTTCAAGGGTACCTGAAGCGAATTGGCACCAAAGCA9810.23582411031087433No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA9740.23414136946258066No Hit
CTGCTTCGAGAATTATTGAAAGGAAGTCATTACATTGTATGTCAGTGTT9650.2319778455147745No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.2286123638181871No Hit
TCGCTGTGAAGATGGCGTCGGGCAGCGGGACAAAAAACTTGGACTTTCGC9450.2271700145196496No Hit
TGTTTAAGTGCTGTGGCCAGAAGCGGGGGG9140.2197178764772061No Hit
TTCGAAATTCCATGAATAAAACAGGTTTTGTGCCTTCTAACTATGTGGAA9070.21803513562891236No Hit
AGGTTAGTTGTTTGTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAATTTC8990.21611200323086244No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG8820.2120253468850063No Hit
ACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTC8810.21178495533525007No Hit
CTCATTGAACTCGCCTGCAGCTCTTGGG8800.21154456378549383No Hit
CTTTCTTTCCGCGCCGATAGCGCTCACGCAAGCATGATTAACGTCCCTA8720.20962143138744385No Hit
CTTCTTGGTGCCTTGGTTCATCCCTAAGGGTCCTAACCGGGGAGTTAT8670.20841947363866267No Hit
CGTCTTGTTCTTGCCTGGTGTCGGTGGTTAGTTTCTGCGACTTGTGTTG8570.20601555814110023No Hit
AAGTTTTTGTGACTGAAGCTAGAGCCGTGACCCAGTCACCTCGGCTTAC8450.2031308595440253No Hit
CTATAATTTTCAACACCTGCTTAGCCTTTTCCAGTGGACACTTCTGTG8400.2019289017952441No Hit
ATTTTACAACATTGAGAGATGATGGATCTCCAACATGGCAGCCTTTTCCT8330.2002461609469504No Hit
TGATAGGACAGTGATCACTGCCGAGGGCCTTGGAACGGATCTTGCTGTCA8300.19952498629768164No Hit
ATCTTAGGAGAGATCGTAGGACATCTGAAGAGCTGCCATGTCTGGGGCA8260.19856342009865668No Hit
ATTCCGTACATACAATGCCTCCATCACGCTACAGCAGCAGCTAAAAGAAC8230.19784224544938797No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC8210.19736146234987545No Hit
ATTGCAAGTGGACTGTGAAATGTATGGGCTTACTAATGACCACTACAGTA8200.19712107080011926No Hit
ACATAATGAGGATATAGTGAGAAGGTGGCTATCTGAAAGCCAAGAAGA8190.196880679250363No Hit
AGACAGTGGTTGTCAGAGTATGATGAAGGTGCTTAAGAATGTTATGGGA8130.19543832995182553No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA8060.19375558910353183No Hit
TGGAAACACTTAAAAAAGCAAAAGGGTGTACAATCATATTTACAGACCGT8050.19351519755377558No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA7990.19207284825523813No Hit
ACGAAACCCCATCTCTACTAAAGATACAAATAATAGCCGGACGTGGTGGC7930.19063049895670067No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG7780.18702462571035702No Hit
TTCTCTTTTGTCTGCTGCCATGTAAGAATGCCTTTCTCCTTCCTCCAT7750.1863034510610883No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA7650.18389953556352587No Hit
AGGTGGTGCGTAATGAAGAACAGAAGGCGGAACAAGAAGAGGGTAAGGTG7310.1757262228718136No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT7290.17524543977230111No Hit
TATTTGAAAAACCATTTCATAACTTTGTCAAAGTTAAATTATAGGCTAA7150.17187995807571374No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC7100.1706780003269325No Hit
AAGAAGCCTTTCACATGCAGCATGTGTAAAAAGTCCTTCAGCCACAAAAC7090.17043760877717626No Hit
TCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAAT7050.1694760425781513No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT7040.16923565102839505No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7020.16875486792888256No Hit
AAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTA7000.16827408482937006No Hit
ATTTTGATGTATATGAAACTTTTGTTAAATATGTTGTATACTTCGCTG6990.16803369327961384No Hit
AAGGTCAGCAGATAAACAAGTGAACAAAGGTCTCTGGTTTTCCTAGGCAG6950.16707212708058886No Hit
CTCTCTCTTTGCCTGTTTCCATCCATGTAAGATGTGACTTTTGCTCCTCC6900.16587016933180765No Hit
TGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTATCTGGCATCA6790.163225862284489No Hit
CTTGTTTCCTGAGTTGTCCTGTGCTGGAGGTCTGCTCAGACGAAGGTCTC6730.16178351298595153No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC6710.161302729886439No Hit
ACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAAT6680.1605815552371703No Hit
AAAACCACACCACCTGCCAGCACTGTTACCGGCAATATCCCACACCCTTC6660.16010077213765783No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTA6550.15745646509033914No Hit
CCTGAAGAGCGGAAGCCTTCTGTCGAGAAGCAGCTACCCAAGCTCCAGGA6540.1572160735405829No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.15697568199082665No Hit
ATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGC6500.1562545073415579No Hit
ATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGA6490.1560141157918017No Hit
TGATCACTGTCCTATCACCCTATACCTAGCACTGTGACACCACCCCTAAA6420.154331374943508No Hit
ATGCATGCATGCTGTTTCTCCTGCCACCTTGTGAAGATGGTGCTTGCTTG6320.15192745944594555No Hit
ATATCAAGTACCTGCAATGTACCTAGCAGTATGCTTTGGATGATACAAAA6320.15192745944594555No Hit
CACAAAGCCTGAAATATTTACTATCTAACTTTTTCTGGGAAAAGTTTGCT6300.15144667634643308No Hit
TTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGA6290.15120628479667683No Hit
AATGGGGTGGGTTTTTTGTATGTTCAAACTGTCATTTTATTTTTACGTTG6280.15096589324692058No Hit
ACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAG6270.15072550169716434No Hit
TTAAAAGGCTTTTGGAACTCTTCTAAACTGGGAGGTCTGATTGAGAAT6230.14976393549813938No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTT6200.14904276084887064No Hit
AGATCGATCTAAGATGGCGACTGTCGAACCGGAAACCACCCCTACTCCTA6190.14880236929911442No Hit
AGCTAAAGGAACTTTGATTACCCACTGCTACAAAGATCTTGGATTCATGG6100.1466388453513082No Hit
CTTTCGGAGGGAATAAAATGGAAGGAGAATCAAGCAGATTTGAAATCCA6090.14639845380155198No Hit
ACGCCGGGGAGAGGTGATGCACTGCCGCTGAAGACCCAGATTGACAAGTA6080.14615806225179573No Hit
AATGAATTCTCTCACCTGTGTGATGATTCTCAAAAGCAAGAGAAGG6080.14615806225179573No Hit
CACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACC6070.14591767070203948No Hit
TTGTCTGTGGCCGGAGGCTGATCAGTGTTCTAGAACAGATCAGACATT6060.14567727915228323No Hit
CTTTCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG6000.14423492985374578No Hit
AATATCAACTATTCTAAATACAGGTAATGGTATATTTAAGGCTACCATAA5990.14399453830398953No Hit
ACGCGTCCCTTTGGGTTTAGCACGATGAGCTCAATCGGCACTGGGTAT5900.14183101435618337No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAAT5850.14062905660740213No Hit
AGAGAAAAGGCCACATAACAACAGAGACAGAGATTGGAATTACGCAGC5830.14014827350788966No Hit
TCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGA5730.13774435801032722No Hit
ATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTC5670.13630200871178977No Hit
AGACTGGCCTACCTTCCCAGCCTACATATTTCTCCCATGCTGGATGC5660.13606161716203352No Hit
TTATTGTAATGATGTTGATCGGGAGTTGAGAAAATGCCTGAAGAATGA5630.13534044251276478No Hit
AGAATTATATGAATAATGTTAAATAAAAGTGCATACAGAACTGAGAAATA5630.13534044251276478No Hit
CGGTCCTCAAGCCTTATTGAACATAAGAGAAGTCATGCTGGAGAGAAA5510.13245574391568987No Hit
CTGGAGAGAACAGGATGCCTCCGTAATTTTCCGGGTCCTTTGTGTT5380.12933065376885872No Hit
AACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTT5340.12836908756983376No Hit
CACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCA5190.1247632143234901No Hit
ACCGCGGCCTCAGATGAATGCGGC5180.12452282277373386No Hit
ATTGCAAGTGGACTGTGAAATGTATGGGCTTACTAATGACCACTACAG5110.12284008192544017No Hit
TTCGAAATTCCATGAATAAAACAGGTTTTGTGCCTTCTAACTATGTGG5090.12235929882592766No Hit
ATTCCGTACATACAATGCCTCCATCACGCTACAGCAGCAGCTAAAAGA5030.12091694952739021No Hit
TCGCTGTGAAGATGGCGTCGGGCAGCGGGACAAAAAACTTGGACTTTC4970.11947460022885277No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA4910.1180322509303153No Hit
CTGCTTCGAGAATTATTGAAAGGAAGTCATTACATTGTATGTCAGTG4900.11779185938055904No Hit
ATTTCTGGCTTTCCATCATGGTTCATACTGAAAGTACTGTAGATGCTAGC4900.11779185938055904No Hit
AAAGTTTGCAAAAAACAATCTATAAGCTGGAAGAACAGCTGCATAA4880.11731107628104656No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT4840.1163495100820216No Hit
CAAAATTAACCCCCTAATAAAATTAATTAACCACTCACTCATCGACCTCC4840.1163495100820216No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTT4820.11586872698250911No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA4760.11442637768397164No Hit
TTTTTCAGCTCACTTCAAGGGTACCTGAAGCGAATTGGCACCAAAG4740.11394559458445916No Hit
AGGTTAGTTGTTTGTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAATT4680.11250324528592172No Hit
CGCGTCTTGTTCTTGCCTGGTGTCGGTGGTTAGTTTCTGCGACTTGTGTT4670.11226285373616546No Hit
AAGCCCCTGCGCCCGCGCCATGTCAAAGAAAAAAGGACTGAGTGCAGA4670.11226285373616546No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGAC4650.11178207063665298No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC4650.11178207063665298No Hit
CTATAATTTTCAACACCTGCTTAGCCTTTTCCAGTGGACACTTCTG4620.11106089598738424No Hit
CTCATTGAACTCGCCTGCAGCTCTTGGGTTTTTTGTGGCTT4620.11106089598738424No Hit
CTTTCCCATCTTGCAGATGGCGGG4590.11033972133811552No Hit
AAGTTTTTGTGACTGAAGCTAGAGCCGTGACCCAGTCACCTCGGCTT4580.11009932978835928No Hit
TTGTCTGTGGCCGGAGGCTGATCAGTGTTCTAGAACAGATCAGACA4570.10985893823860304No Hit
TCCCTTTCTCCATAAAATTCTTCTTAGTAGCTATTACCTTCTTATTATTT4570.10985893823860304No Hit
AGACAGTGGTTGTCAGAGTATGATGAAGGTGCTTAAGAATGTTATGG4540.10913776358933432No Hit
ATTTTACAACATTGAGAGATGATGGATCTCCAACATGGCAGCCTTTTC4520.10865698048982184No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.10865698048982184No Hit
TATATGTGATACTAAATCCATTGATCCTGCTTTTCTTTAAAGTGGTGAT4500.10817619739030934No Hit
ACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCAT4480.10769541429079685No Hit
ACGAAACCCCATCTCTACTAAAGATACAAATAATAGCCGGACGTGGTG4410.10601267344250315No Hit
TTCTCTTTTGTCTGCTGCCATGTAAGAATGCCTTTCTCCTTCCTCC4400.10577228189274691No Hit
CTTTCTTTCCGCGCCGATAGCGCTCACGCAAGCATGATTAACGTCCC4400.10577228189274691No Hit
ACATAATGAGGATATAGTGAGAAGGTGGCTATCTGAAAGCCAAGAA4370.10505110724347817No Hit
CACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGA4350.10457032414396569No Hit
ATGCATGCATGCTGTTTCTCCTGCCACCTTGTGAAGATGGTGCTTGCT4340.10432993259420945No Hit
ATCTTAGGAGAGATCGTAGGACATCTGAAGAGCTGCCATGTCTGGGG4340.10432993259420945No Hit
ATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCC4340.10432993259420945No Hit
CTAACATTTTAAAAATTGATTCACCAATAAGATCTCATTAAGATACTT4240.10192601709664703No Hit
CTTGTTTCCTGAGTTGTCCTGTGCTGGAGGTCTGCTCAGACGAAGG4200.10096445089762204No Hit
ACGCGTCCCTTTGGGTTTAGCACGATGAGCTCAATCGGCACTGGGT4170.10024327624835333No Hit
TGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTATCTGGCAT4160.10000288469859707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG600.0274.5769344
ATAGAGT350.0274.576944
ATATCAG350.0274.576944
CCTCCGA100.0010467299274.576944
AATGAGC1000.0274.576944
CAGATAC208.7717126E-8274.576944
AGACCGT800.0274.576944
CGTCGCC100.0010467299274.576944
TACGTTG700.0274.576944
GGTATGA1100.0262.0961644
ACAAGTA500.0247.1192344
CCCTAAA900.0244.0683744
ACTCCTA700.0215.7390144
AGGGAAG650.0211.2130144
TAGGCAG1050.0209.2014844
AATTGTC600.0205.9327144
CGTAAAT1250.0197.6953744
GTTTGCT1100.0187.2115544
GAGTCCC154.4960558E-5186.498843
GGGTATG1100.0186.498843
Produced by FastQC (version 0.11.5)