FastQCFastQC Report
Tue 24 May 2016
ERR841073.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841073.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1553619
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT83870.5398363434020825No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA59890.38548704669548967No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC57520.37023234139129346No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC47760.3074112765098779No Hit
CCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGC41870.26949979370746624No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA33190.2136302401039122No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA32570.20963955770365836No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG27220.1752038305401775No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC26280.16915344109463132No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA26030.1675442949654967No Hit
CCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC23920.1539631016356005No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG22860.1471403220480697No Hit
TGACCCGGCGTGGCTACTAGGAGAAGGACGTACGGTCCTGCTAGTAGA22230.14308527380265046No Hit
CTCTTTCCGCCATCTTTCCGCGCCGCCACAATGGTGCGCATGAATGTCC22050.14192668858967353No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA21410.1378072744990889No Hit
TCAGGCTCTGGGTGTCAACCTCTGACTTATTCTGCAGATGCTCTGTGTGT20960.1349108114666466No Hit
CTCTTACCGCCATCTTGGCTCCTGTGGAGGCCTGCTGGGAACGGGACTT20930.13471771393115042No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20020.12886042202110043No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT19600.12615705652415424No Hit
TGCTATCTCTGGGGATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTG19170.1233893251820427No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCG19090.12287439842071962No Hit
ACCTTTCCGAGTTGGCTGCAGATTTGTGGTGCGTTCTGAGCCGTCTGT18840.121265252291585No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTTC18660.12010666707860808No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG17970.11566542376219653No Hit
TGACTAGCCTGGGCAATATGGCAAGACCTTGTCTCTACAAAAAATTTCA17970.11566542376219653No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA17930.11540796038153499No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17810.11463557023955037No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT17640.11354135087173882No Hit
CTATACATGTATGCAATGTGTAATGATCAAATCAGGGTAATTGACATCTC17540.11289769242008499No Hit
AGCTGCGGTGGCGTCTCCACACGCAACCATGAAGTTGAAGGACACAAA17520.1127689607297542No Hit
TGGTTCAATCTCTGGTAATCTATGCCAGCAATTATGACAATGTTAGCAGA16940.10903574171016188No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT16680.10736222973586189No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC16440.10581744945189266No Hit
CTTTTTGTCCGACATCTTGACGAGGCTGCGGTGTCTGCTGCTATTCTCC15840.10195549874196955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCT156.478395E-5170.2898344
GACCGCA600.0170.2898344
CCGCAAT500.0170.2898344
CCGATCC100.004383206170.2898144
ATATATG950.0170.2898144
CCAAGTA850.0170.2898144
AGATTGT1250.0163.4782144
GTAACAG850.0160.2727744
AGCCTAT1450.0158.5456844
GCGTGCC700.0158.1262744
CCCATAC700.0158.1262744
CTGAATC550.0154.8089344
TCGGCTT1050.0154.0717344
GGAGTAG1050.0154.0717344
ACTGATT500.0153.2608344
CCCGCCA1000.0153.2608344
TCCGGAC950.0152.3645644
AACGTGA850.0150.255744
TCTTCTA409.094947E-12149.0035944
ATCAGTA409.094947E-12149.0035944
Produced by FastQC (version 0.11.5)