##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841073.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1553619
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.760280995533655	39.0	38.0	39.0	35.0	39.0
2	38.08822433299284	39.0	38.0	39.0	35.0	39.0
3	38.16580577348758	39.0	38.0	39.0	35.0	39.0
4	38.615401845626245	39.0	38.0	40.0	35.0	41.0
5	39.37655564202034	40.0	39.0	41.0	36.0	41.0
6	39.43391140298876	40.0	39.0	41.0	36.0	41.0
7	39.43140628429493	40.0	39.0	41.0	36.0	41.0
8	39.37979646232442	40.0	39.0	41.0	36.0	41.0
9	39.2612410121143	40.0	39.0	41.0	35.0	41.0
10	39.237314296490965	40.0	39.0	41.0	35.0	41.0
11	39.17029464752942	40.0	39.0	41.0	35.0	41.0
12	39.113260715786815	40.0	39.0	41.0	35.0	41.0
13	38.93959201065383	40.0	38.0	41.0	35.0	41.0
14	38.9156614330798	40.0	38.0	41.0	35.0	41.0
15	38.96321813778024	40.0	38.0	41.0	35.0	41.0
16	38.95377824292829	40.0	38.0	41.0	35.0	41.0
17	39.11011386961668	40.0	38.0	41.0	35.0	41.0
18	39.08756908868905	40.0	38.0	41.0	35.0	41.0
19	39.04518611062301	40.0	39.0	41.0	35.0	41.0
20	39.03279632908712	40.0	38.0	41.0	35.0	41.0
21	39.124744869881226	40.0	38.0	41.0	35.0	41.0
22	39.1362547703137	40.0	38.0	41.0	35.0	41.0
23	39.07862802913713	40.0	38.0	41.0	35.0	41.0
24	39.008852878344044	40.0	38.0	41.0	35.0	41.0
25	38.94016709038106	40.0	38.0	41.0	35.0	41.0
26	38.92636887888479	40.0	38.0	41.0	35.0	41.0
27	38.91904519591063	40.0	38.0	41.0	35.0	41.0
28	38.845179330781384	40.0	38.0	41.0	35.0	41.0
29	38.828255158040164	40.0	38.0	41.0	35.0	41.0
30	38.724289287532194	40.0	38.0	41.0	35.0	41.0
31	38.63374625477769	40.0	38.0	41.0	35.0	41.0
32	38.500330871503955	40.0	37.0	41.0	34.0	41.0
33	38.35805332180068	40.0	37.0	41.0	34.0	41.0
34	38.389592791502736	40.0	37.0	41.0	34.0	41.0
35	38.46234722224172	40.0	37.0	41.0	34.0	41.0
36	38.46388548581966	40.0	37.0	41.0	34.0	41.0
37	38.45314351400287	40.0	37.0	41.0	34.0	41.0
38	38.382939028008735	40.0	37.0	41.0	34.0	41.0
39	38.35248490889643	40.0	37.0	41.0	34.0	41.0
40	38.332196885013786	40.0	36.0	41.0	34.0	41.0
41	38.27658688790953	40.0	36.0	41.0	34.0	41.0
42	38.21972645118096	40.0	36.0	41.0	34.0	41.0
43	38.153011870017004	40.0	36.0	41.0	34.0	41.0
44	38.011618230536314	39.0	35.0	41.0	34.0	41.0
45	37.84849699761256	39.0	35.0	41.0	34.0	41.0
46	37.51062547049603	39.0	35.0	41.0	33.0	41.0
47	37.35564522953549	39.0	35.0	41.0	33.0	41.0
48	37.32527999964081	39.0	35.0	41.0	33.0	41.0
49	37.503185781073725	39.0	35.0	41.0	33.0	41.0
50	37.29693934638739	38.0	36.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	6.0
22	13.0
23	37.0
24	70.0
25	145.0
26	340.0
27	650.0
28	1332.0
29	3049.0
30	5907.0
31	10926.0
32	19138.0
33	33365.0
34	67922.0
35	60278.0
36	91628.0
37	164109.0
38	202553.0
39	345762.0
40	546388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	50.37721603559174	17.346209077000214	32.276574887408046
2	26.19162098300806	21.640569534744362	31.917284739694868	20.250524742552713
3	23.517220116386323	19.43970819100436	31.553167153594288	25.489904539015036
4	20.959965087965582	18.506660899486942	39.658436206045366	20.874937806502107
5	17.0591374075626	24.052422118936494	34.593938410897394	24.29450206260351
6	19.715515837538035	29.338982079905048	28.85836231405512	22.0871397685018
7	25.302213734512772	26.313658625441633	24.57410729400194	23.810020346043657
8	26.85755001708913	26.004766934492952	26.79871963460797	20.33896341380995
9	25.483467954498494	22.724232903948778	28.032484154738068	23.759814986814657
10	23.283314635055312	26.581742370555457	26.900546401659607	23.23439659272962
11	27.444888354223266	19.990357996394227	30.145293022291824	22.419460627090686
12	25.900816094550848	23.203372255359906	28.03847017833845	22.857341471750797
13	26.810820413499066	20.47355239605077	30.127656780716507	22.58797040973366
14	22.518197833574384	25.44896786148985	27.62047837983444	24.412355925101327
15	24.452584578329695	25.650561688547835	28.141455530603064	21.75539820251941
16	18.763931182612982	31.039785172555177	28.094275366096838	22.102008278735006
17	23.567940402376646	31.927969469992323	25.51500721862954	18.989082909001496
18	23.108561365431292	26.76421954159932	26.36682481354824	23.760394279421146
19	26.034568320804524	25.746659895379754	23.93141433002557	24.28735745379015
20	23.012720621980034	25.435322302314788	26.91303337562169	24.63892370008348
21	23.924140989521884	25.46248468897458	27.19341099716211	23.419963324341424
22	25.053761572174388	23.449893442343328	25.74678862707009	25.749556358412196
23	25.65023985932201	26.103568506821812	27.23344655285498	21.012745081001196
24	26.460863313334865	24.443058433245216	27.261896256418076	21.83418199700184
25	22.979838777427805	30.052357915661233	24.08722614199322	22.88057716491774
26	24.74231150954534	28.81823312294449	26.338326014050427	20.10112935345974
27	23.37863978988889	26.759520903931243	26.17938281490767	23.68245649127219
28	22.083664117337275	26.86431174656574	26.678810697227178	24.373213438869808
29	25.86534189853844	27.983265948036646	25.184458186621463	20.96693396680346
30	26.67057331502562	29.2127926585096	23.813256014435574	20.303378012029203
31	26.23314943277774	27.788158359631183	23.52639874708947	22.452293460501608
32	24.42342829220554	28.96921429773801	27.247523915773954	19.3598334942825
33	23.193444216716355	26.29361045125679	27.763257500576284	22.749687831450572
34	24.12094523776728	26.610035932012355	28.665970727191493	20.603048103028875
35	21.681632698897094	25.517868698509666	27.919850194626267	24.880648407966973
36	21.16301790288541	27.941908315910712	30.845082733351468	20.0499910478524
37	25.060129588287506	28.169738855676247	26.904787858282454	19.865343697753794
38	23.435817851788446	28.010086930039712	24.666443240027807	23.887651978144035
39	19.403010651955668	26.558414388065927	30.94579484096326	23.092780119015146
40	22.865039238278676	29.052305284699653	26.947224658492264	21.13543081852941
41	21.138446938379357	29.048765933584004	27.95101119394937	21.861775934087266
42	20.350297746591462	30.01995558691502	26.202205070710306	23.42754159578321
43	24.579358900192975	25.645007184064283	25.168945124187896	24.606688791554845
44	21.782389092886557	28.91280911014484	27.18280964443413	22.12199215253447
45	23.088163479393252	26.25996274145706	29.248709817445196	21.40316396170449
46	21.357179402783707	30.6158080148082	25.773161440019294	22.2538511423888
47	22.466438023373343	30.44296389407356	26.02920384021846	21.061394242334632
48	23.08613594883342	27.95102447653705	26.854261000747563	22.108578573881964
49	22.21061859973146	29.087580901741156	26.217444435318	22.484356063209383
50	19.868458756907962	28.963635123149352	32.21750699324555	18.95039912669714
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11387.0
1	11745.5
2	15314.0
3	18284.5
4	17396.0
5	11807.5
6	4926.0
7	3527.0
8	4221.5
9	4626.5
10	4897.0
11	4976.5
12	5358.0
13	6010.0
14	6649.5
15	6754.0
16	5775.5
17	4725.5
18	5319.5
19	6667.5
20	7307.0
21	8532.5
22	10535.0
23	13627.0
24	16640.5
25	20374.5
26	26237.0
27	33160.5
28	36149.5
29	33891.5
30	34975.5
31	38062.5
32	42173.0
33	51315.5
34	58401.0
35	57759.5
36	60740.5
37	72093.5
38	81078.5
39	82349.5
40	83083.0
41	91350.0
42	96384.5
43	91096.0
44	90775.5
45	91942.5
46	95049.5
47	110482.5
48	125521.5
49	130223.5
50	133069.0
51	131493.0
52	121595.0
53	112755.5
54	107547.5
55	102447.0
56	102270.5
57	102135.5
58	98302.0
59	91282.5
60	75036.0
61	60634.0
62	51793.0
63	41578.5
64	31307.0
65	25880.5
66	20033.5
67	13868.5
68	9730.0
69	6878.0
70	5288.0
71	3494.5
72	2203.5
73	1779.5
74	1475.5
75	1091.0
76	737.0
77	416.5
78	303.0
79	306.0
80	254.5
81	236.5
82	161.0
83	56.5
84	21.5
85	15.0
86	13.5
87	17.0
88	16.0
89	16.0
90	9.5
91	2.5
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	6193.0
25	5836.0
26	6783.0
27	8131.0
28	9530.0
29	10313.0
30	15266.0
31	24230.0
32	31414.0
33	5722.0
34	5419.0
35	6128.0
36	5872.0
37	7399.0
38	4889.0
39	4918.0
40	4646.0
41	4852.0
42	6636.0
43	13141.0
44	39357.0
45	99516.0
46	175717.0
47	160817.0
48	294337.0
49	230132.0
50	366425.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.1027107874128355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.33915170053011	4.782905191985846
2	12.47775494542159	1.772517693070796
3	5.196481581138027	1.107273173488235
4	2.90146162469854	0.8243297112404278
5	1.7302899273095596	0.614487446602669
6	1.1228741624832812	0.47852702560662935
7	0.7994257144561576	0.39746627520620753
8	0.6027851993067966	0.3425127150087345
9	0.4851685592240023	0.31014107633824783
>10	4.042152429639481	6.070071721605123
>50	0.8049387424802987	4.105518586301934
>100	1.6999277095759733	30.72791881741905
>500	0.624838293249547	30.77360458183223
>1k	0.16999277095759735	16.37906446128161
>5k	0.002756639529042119	1.313661523012246
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	8387	0.5398363434020825	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	5989	0.38548704669548967	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	5752	0.37023234139129346	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	4776	0.3074112765098779	No Hit
CCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGC	4187	0.26949979370746624	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	3319	0.2136302401039122	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	3257	0.20963955770365836	No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG	2722	0.1752038305401775	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	2628	0.16915344109463132	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	2603	0.1675442949654967	No Hit
CCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC	2392	0.1539631016356005	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	2286	0.1471403220480697	No Hit
TGACCCGGCGTGGCTACTAGGAGAAGGACGTACGGTCCTGCTAGTAGA	2223	0.14308527380265046	No Hit
CTCTTTCCGCCATCTTTCCGCGCCGCCACAATGGTGCGCATGAATGTCC	2205	0.14192668858967353	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	2141	0.1378072744990889	No Hit
TCAGGCTCTGGGTGTCAACCTCTGACTTATTCTGCAGATGCTCTGTGTGT	2096	0.1349108114666466	No Hit
CTCTTACCGCCATCTTGGCTCCTGTGGAGGCCTGCTGGGAACGGGACTT	2093	0.13471771393115042	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2002	0.12886042202110043	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1960	0.12615705652415424	No Hit
TGCTATCTCTGGGGATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTG	1917	0.1233893251820427	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCG	1909	0.12287439842071962	No Hit
ACCTTTCCGAGTTGGCTGCAGATTTGTGGTGCGTTCTGAGCCGTCTGT	1884	0.121265252291585	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTTC	1866	0.12010666707860808	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG	1797	0.11566542376219653	No Hit
TGACTAGCCTGGGCAATATGGCAAGACCTTGTCTCTACAAAAAATTTCA	1797	0.11566542376219653	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	1793	0.11540796038153499	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1781	0.11463557023955037	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	1764	0.11354135087173882	No Hit
CTATACATGTATGCAATGTGTAATGATCAAATCAGGGTAATTGACATCTC	1754	0.11289769242008499	No Hit
AGCTGCGGTGGCGTCTCCACACGCAACCATGAAGTTGAAGGACACAAA	1752	0.1127689607297542	No Hit
TGGTTCAATCTCTGGTAATCTATGCCAGCAATTATGACAATGTTAGCAGA	1694	0.10903574171016188	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	1668	0.10736222973586189	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	1644	0.10581744945189266	No Hit
CTTTTTGTCCGACATCTTGACGAGGCTGCGGTGTCTGCTGCTATTCTCC	1584	0.10195549874196955	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.04615031098358092	0.0	0.0	0.0	0.0
2	0.07234720996589254	0.0	0.0	0.0	0.0
3	0.09468215823828108	0.0	0.0	0.0	0.0
4	0.12956844631791964	0.0	0.0	0.0	0.0
5	0.14874946817720433	0.0	0.0	0.0	0.0
6	0.23049409153724304	0.0	0.0	0.0	0.0
7	0.2583645024938547	0.0	0.0	0.0	0.0
8	0.292542766276674	0.0	0.0	0.0	0.0
9	0.3268497617498241	0.0	0.0	0.0	0.0
10	0.3580671966550358	0.0	0.0	0.0	0.0
11	0.39173053367653204	0.0	0.0	0.0	0.0
12	0.4215962858332706	0.0	0.0	0.0	0.0
13	0.4516551355255053	0.0	0.0	0.0	0.0
14	0.48866549649560154	0.0	0.0	0.0	0.0
15	0.5211702483041208	0.0	0.0	0.0	0.0
16	0.5538680976481364	0.0	0.0	0.0	0.0
17	0.6023355790576711	0.0	0.0	0.0	0.0
18	0.6338104773435443	0.0	0.0	0.0	0.0
19	0.6694691555651675	0.0	0.0	0.0	0.0
20	0.7035186876576561	0.0	0.0	0.0	0.0
21	0.7348004884080331	0.0	0.0	0.0	0.0
22	0.7615766799968332	0.0	0.0	0.0	0.0
23	0.7918929930697295	0.0	0.0	0.0	0.0
24	0.8206645258586565	0.0	0.0	0.0	0.0
25	0.8494360586475835	0.0	0.0	0.0	0.0
26	0.8773064696041951	0.0	0.0	0.0	0.0
27	0.9024735150638605	0.0	0.0	0.0	0.0
28	0.9312450478527876	0.0	0.0	0.0	0.0
29	0.9613038975450223	0.0	0.0	0.0	0.0
30	0.9882088208241532	0.0	0.0	0.0	0.0
31	1.013118402903157	0.0	0.0	0.0	0.0
32	1.0414393747759263	0.0	0.0	0.0	0.0
33	1.069631614958365	0.0	0.0	0.0	0.0
34	1.096085977321338	0.0	0.0	0.0	0.0
35	1.121832315387492	0.0	0.0	0.0	0.0
36	1.144424727040542	0.0	0.0	0.0	0.0
37	1.160644920022219	0.0	0.0	0.0	0.0
38	1.173839918281123	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCCT	15	6.478395E-5	170.28983	44
GACCGCA	60	0.0	170.28983	44
CCGCAAT	50	0.0	170.28983	44
CCGATCC	10	0.004383206	170.28981	44
ATATATG	95	0.0	170.28981	44
CCAAGTA	85	0.0	170.28981	44
AGATTGT	125	0.0	163.47821	44
GTAACAG	85	0.0	160.27277	44
AGCCTAT	145	0.0	158.54568	44
GCGTGCC	70	0.0	158.12627	44
CCCATAC	70	0.0	158.12627	44
CTGAATC	55	0.0	154.80893	44
TCGGCTT	105	0.0	154.07173	44
GGAGTAG	105	0.0	154.07173	44
ACTGATT	50	0.0	153.26083	44
CCCGCCA	100	0.0	153.26083	44
TCCGGAC	95	0.0	152.36456	44
AACGTGA	85	0.0	150.2557	44
TCTTCTA	40	9.094947E-12	149.00359	44
ATCAGTA	40	9.094947E-12	149.00359	44
>>END_MODULE