FastQCFastQC Report
Tue 24 May 2016
ERR841072.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841072.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121022
Sequences flagged as poor quality0
Sequence length24-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC201171.7945232118548968No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT173521.5478732799177892No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG143831.283025667649698No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA114431.0207649805266978No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC96480.860643234477111No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA94560.8435160059303028No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT78230.6978453589670853No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT60330.5381696344942383No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC54110.48268455034780766No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA53640.4784919475264536No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT41370.3690382525945075No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC39430.3517326154170034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA38050.33942241989898503No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA36610.3265769984888789No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA35680.3182809971615187No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA33540.2991912736770554No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT31280.2790310984084166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA30320.27046748413501254No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA28360.2529834383268125No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA27880.24870163119011046No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT27470.24504425426084414No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC26890.2398704039706625No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG25100.22390283152337775No Hit
AAAAAAAAAAAAAAAAAAAAAAAA23170.20668639866122163No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC21480.1916108693674165No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG20930.18670463202327875No Hit
ACCTAAAATCGCTCATTGCATACTCTTCAATCAGCCACATAGCCCTCGT19660.17537568397408793No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGAC19140.17073705957599405No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT18550.16547400497046444No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG18540.1653848006551165No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18530.16529559633976854No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC18390.1640467359248971No Hit
AGTCCGAACTAGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTC17930.159943337418891No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA16670.14870359368504812No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTA16590.14798995916226443No Hit
CACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG15190.13550135501355012No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA15180.13541215069820217No Hit
AAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATC15010.13389567733728686No Hit
ATTCTTAATTTAACTATTTATATTATCCTAACTACTACCGCATTCCTACT14970.13353886007589502No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA14890.13282522555311135No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC14560.1298814831466287No Hit
CTGGAGATGAAAATTTTGAAATCCTTAGCATGTTGATGGAATTTAAA14290.12747296663223381No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT14270.12729455800153788No Hit
TCAACCTCAACCTAGGCCTCCTATTTATTCTAGCCACCTCTAGCCTAG14100.12577808464062257No Hit
ATCTCAGCAATTTGCCGCATTCTGGCTATTCTGATAGTGCTGTTCTCAAG13950.12444001991040318No Hit
TACTCAAGTCTACCATTGTGGTGCTAAATCAAAGCTAGACAATATGTGTA13810.12319115949553175No Hit
AGTCCTTTAGGTAAGAAGTATGGAGAGACAGCTAATGAGTGTGGAGAAGC13600.1213178688732246No Hit
AGTCCTCACTGAGAAAATCACTCCTCTGGAGATTGAGGTGCTGGAAGAAA13500.12042582571974501No Hit
CCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGG13440.11989059982765726No Hit
AGATTATTCCGAAGCCTGGTAGGATAAGAATATAAACTTCAGGGTGA13320.11882014804348175No Hit
ACGGGGGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATTTGGT13300.11864173941278583No Hit
AAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACT13120.11703606173652256No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA12800.11418152364538786No Hit
AAGGCGACAGTGCCTAAGACAGAAATTCGGGAAAAACTAGCCAAAAT12750.11373550206864808No Hit
AGTTCCTGGAGTTTGAGTTTCGACCGGACGGGAAGTTAAGATATGCCAA12750.11373550206864808No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCT12740.11364629775330011No Hit
TGACAGCATTTGGGGAACTAAAAGATTCATGTGTTTCAGCCTAGTGGGA12590.11230823302308071No Hit
CAGTTCTATCGCTACAAGATGCCCCGTCTGATTGCCAAGGTTGAGGGCAA12520.11168380281564501No Hit
CTTTCCTCATGCAAACACTGCATGTTCCAGTTATTTGCTTCCTGAGGCAG12220.10900767335520624No Hit
CACTGTGTACCCATCTCTGCCCATCACCGCTGGAATTTTGATGACCTATT12190.10874006040916236No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA12150.10838324314777052No Hit
AATTCATTCATGATCATGCTGGTGAAGTAGTCACATGCAGAGATGAGATC11970.10677756547150725No Hit
ACTTCGCTGTGAAGATGGCGTCGGGCAGCGGGACAAAAAACTTGGACTT11940.10650995252546337No Hit
AGCCACAGACTCCTAAGGAAAAGGCTGAGGCTCTAGAGGACCTGGTTGGC11890.10606393094872357No Hit
AAGTAAGAGCAGAAAAAGCCCATCCAGTAATGCCTGAATTTCAAGCCTCA11770.10499347916454807No Hit
AGACTTTTCATTTTGTTCTGTACTAAGAAAAATTCTTCTGCCTTGGGAT11660.10401223169572052No Hit
ATCTCCTCAGAGGATCAGGCGTGTGTCCAGTTCTGGAAAGCCTACATCCT11630.10374461874967664No Hit
AGGGTTAAATGGATTAAGGGCAGTGCAAGATGTGCTTTGTTAAACAGATG11610.1035662101189807No Hit
AGTCTCAATTTAGGGAAAGGACAGGCAGTCTATTCCCTCCAATATAATAC11600.10347700580363277No Hit
CAAGACCAGTGTGACCAACATGGTGAAACCTTGTCTCTACTAAAAATACA11520.10276337128084909No Hit
ACTCACGGTTAAATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACA11250.10035485476645419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACT252.0316293E-8160.3430344
GGAATCC252.0316293E-8160.3430344
CGTGCGC158.2325496E-5160.3430344
ATTACTG450.0160.3430344
ATTACGA500.0160.3430344
CGGCAAA850.0160.3430344
CCAATGT500.0160.3430344
GAATCGT650.0160.3430344
GCGCCAG600.0160.3430344
GCTTGAC1150.0160.3430344
CCGTCTC201.2897199E-6160.3430344
TAGTTTC158.2325496E-5160.3430344
CGTTAGG1150.0160.3430344
GTTACAA550.0160.3430344
GATGGGG100.005247066160.3430344
TTGAATG900.0160.3430344
TATAGTC800.0160.3430344
TGGGATG600.0160.3430344
TTGCAAT1100.0160.3430344
TAATGGC355.456968E-12160.3430344
Produced by FastQC (version 0.11.5)