FastQCFastQC Report
Tue 24 May 2016
ERR841071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8490471
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA973781.146909282182343No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC706810.8324744292748895No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT524520.6177749149605481No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA406450.478713136173482No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC381590.44943325287843283No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA247680.29171526526620256No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT210420.24783077405246423No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA200500.23614708771751294No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA196240.23112969822286655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA180880.21303882905907104No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT177310.20883411532764204No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA160870.18947123192576712No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA145700.1716041430445967No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT135550.15964956478857298No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT133170.15684642230095364No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA130760.15400794608449875No Hit
AAAAAAAAAAAAAAAAAAAAAAAA113540.13372638573289986No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA110910.13062879550498435No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110440.13007523375322758No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGA101100.11907466617576339No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT97180.11445772560791975No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT95280.112219922781669No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGA91010.10719075537741075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTG700.0142.9915344
CAGTACG1000.0135.8419644
AGCGATT1400.0132.7778544
TCGATTG1800.0127.10358444
CACGCGG1100.0123.4926844
TTGACGC650.0120.99283644
CCGTTCA650.0120.99283644
GGCACTA800.0116.1806244
GCCGGCA1650.0112.6644
GCCCGCT2050.0111.6031544
TGCCGGC700.0102.1368144
GCAACCC850.0100.935244
GTAAGAG850.0100.935244
CGTAACT1500.0100.0940844
TATTGCG650.098.5178643
ATTGACG550.098.5178643
GCGTAAC1050.098.5178643
TAGGCGT451.2034434E-895.3276844
GTTACCT1050.093.8265343
CGATATT1050.093.8265343
Produced by FastQC (version 0.11.5)