FastQCFastQC Report
Tue 24 May 2016
ERR841070.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841070.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202502
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC241692.009892707039157No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA141721.1785427383904559No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA111190.924655426768521No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT92130.7661525718876143No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA90620.7535954202155173No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA79990.6651963988417483No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT71150.5916830075958294No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG62270.5178369765705172No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA52330.4351759913912825No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC50930.42353359911251703No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC45980.3823694264125964No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC44640.37122599380292093No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG37610.3127645525745487No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG37020.3078581158284976No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC36590.30428223820001965No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA28460.23667320303833173No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA27760.23085200689894905No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT25040.20823250190020473No Hit
TCCTTACCCTGCCATTTCTAGTTACGTCAAGGATCTTACTGCTCCAGGA23810.19800382868386085No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT23130.19234895243417477No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT22940.19076891348205657No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG21900.18212027921783083No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT21140.17580012340935813No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC20750.1725568855602735No Hit
CTTTCTAACTCCGCTGCCGCCATGGCTCCTGTGAAAAAGCTTGTGGTGA19800.16465669079968265No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA18750.1559248965906086No Hit
CCTTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGC17800.14802470183001773No Hit
AACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTAT17740.14752574216092781No Hit
CACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG15920.13239063219853273No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA15760.13106007308095952No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA15460.1285652747355098No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA15430.1283157949009648No Hit
CTAACAGGGCCAATCACCAGTGACCAGCTAGACTGTGATTTTGATAGCGT15380.12789999517672318No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG15150.12598731644521174No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG14960.12440727749309358No Hit
CCCAAGATGGCGGCCACCATGAAGAAGGCGGCTGCAGAAGATGTCAATGT14430.11999980041613237No Hit
ACAAGAGGAGAGAGTGATATGATGGCATAAGCAGGTGTGGAAGAGATGT14380.11958400069189074No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACAC13960.1160912830082611No Hit
AGACAAGGAGAGCATCGCCAGGGCGGAGGGATCATGGCACAAGTTGCAG13740.11426176422159796No Hit
CTTTTTGTCCGACATCTTGACGAGGCTGCGGTGTCTGCTGCTATTCTCC13550.11268172526947981No Hit
CTCCTGTGGAGGCCTGCTGGGAACGGGACTTCTAAAAGGAACTATGTC13520.11243224543493484No Hit
ACATTTTCTGGTGATACCCAAGAAACATATATCCCAGATTTCTGTGGCAG13180.1096048073100918No Hit
ACACTGGGGCTGGGTAGAAGACTGGTTCTCAGGCTGATGCAGAAATCC13070.10869004791676024No Hit
CACTTATGTACTGCATCAAGCCGTCCTCAGAGGTGAGGAAGGTGCTGTCA13070.10869004791676024No Hit
TCCTTACCCTGCCATTTCTAGTTACGTCAAGGATCTTACTGCTCCAG13050.10852372802706356No Hit
ACTTCCGGTCGGCGTGAGCGTGAGGTGTGGGTGTTCGTTTCTCAGGTAA13030.10835740813736693No Hit
ATTTTCGTTGGTGGTGTTCAGTTGTGGCGG12950.10769212857858032No Hit
AGCCCTCTCCCGTCTCCTCCTCTTCCTGGATCTGGACAGTGCGGGCAGC12930.10752580868888367No Hit
TGGGTGGGATGGAGGCGACCTTGGAGCAGCACTTGGAAGACACAATGAA12920.10744264874403536No Hit
ACCCAAAGCCCTGGTGCCTGGAACTACTGCTTGATTCTCTTTAGAGACC12910.10735948879918704No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGC12860.1069436890749454No Hit
CCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATTATTATAAT12740.10594576973676552No Hit
AGTGATTTACCTGTGCCCCGGACACCTGGCTCAGGGGCAGGGGAAAAACA12720.10577944984706887No Hit
CTGGGCGACCACTTCCTGACAGAAGAAAAGATGTGGAGTGGGCTGCTA12660.10528049017797891No Hit
AAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTG12660.10528049017797891No Hit
TACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACC12530.1041994108949507No Hit
ATATCACATATCTATATTCAGTTCGTGAAGCAAGAAATTGACAGTGTTA12470.10370045122586075No Hit
CTCTTGAGCTGCTTGCTTGGCCCACTCTCACTCTGTGGAGTGTACATTT12460.10361729128101242No Hit
CGAAACCCTGCTCCTACTAAAAATACAAAAATTGGCTGGGTGTGGTGG12390.10303517166707415No Hit
AGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGC12100.10062353326647275No Hit
CCTTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAA12060.10029089348707944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGA450.0222.545344
ATTAGTC400.0222.545344
GTTTACA1200.0222.545344
ATAGCGT1400.0222.545344
TTACTTG450.0222.545344
ATTAAGC650.0222.545344
TGAGTTA152.2271055E-5222.545344
CCCGTAC202.5173722E-7222.545344
TCGGAAA850.0222.545344
ACAATCT600.0222.545344
GGACCAC1000.0222.545344
CAACTCG800.0222.545344
GCTGTCA1350.0222.545344
CAATCTC252.8612703E-9222.545344
TCGGTGC650.0222.545344
CGTGCAT850.0209.454444
GAAGTTC850.0209.454444
AAGGCGA800.0208.6362344
GTAACAA800.0208.6362344
CCCATAT700.0206.6492244
Produced by FastQC (version 0.11.5)