FastQCFastQC Report
Tue 24 May 2016
ERR841069.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841069.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35364521
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA1148820.3248509996784631No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC1064630.30104465433025374No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG1002630.2835129592169508No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC623380.17627271128598065No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG619710.17523494804298353No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT607220.17170315978548106No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA594300.16804978073928953No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG574470.1624424659957928No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC522690.1478006728834246No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG512350.1448768385693673No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT413200.1168402648518836No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG386830.10938363904320944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT350250.0138.3491444
ACTTCTA291250.097.6427744
ACCACTC54400.079.33105544
CCCATAC130350.076.0221244
AACGACA86700.074.119643
TCCGGGC58200.071.8514843
CCTATCA342100.067.9407343
CCTTAGC348750.066.5171643
AATCGCA18400.066.28416444
TATCGCC4950.063.96653744
CGGTCCG351.254605E-463.8070743
CCCTATC390150.061.30729742
ACCTTAG275550.057.5931642
GAACACA204350.057.445244
TCGTGCG19650.056.82563843
TCCAGAA245350.055.04611643
CAATGAA300050.052.64003843
ATACGTC5800.048.52634444
AACCTTA420700.047.98344841
GACGGGC11700.047.71896743
Produced by FastQC (version 0.11.5)