##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841069.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35364521
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.50939909521183	39.0	37.0	39.0	35.0	39.0
2	37.97110541946829	39.0	38.0	39.0	35.0	39.0
3	38.21551794805873	39.0	38.0	39.0	35.0	40.0
4	38.729286761723706	39.0	38.0	41.0	35.0	41.0
5	39.35128715584752	40.0	39.0	41.0	35.0	41.0
6	39.37083018882117	40.0	39.0	41.0	36.0	41.0
7	39.37915977993877	40.0	39.0	41.0	36.0	41.0
8	39.291300736124775	40.0	39.0	41.0	35.0	41.0
9	39.29159128155589	40.0	39.0	41.0	35.0	41.0
10	39.27686853159979	40.0	39.0	41.0	35.0	41.0
11	39.18878536485762	40.0	39.0	41.0	35.0	41.0
12	39.12569306396091	40.0	39.0	41.0	35.0	41.0
13	39.10997683243045	40.0	38.0	41.0	35.0	41.0
14	39.080286821925284	40.0	38.0	41.0	35.0	41.0
15	39.065423818408284	40.0	38.0	41.0	35.0	41.0
16	39.11598375671482	40.0	38.0	41.0	35.0	41.0
17	39.15704886827111	40.0	39.0	41.0	35.0	41.0
18	39.15105975845113	40.0	39.0	41.0	35.0	41.0
19	39.1620508305485	41.0	39.0	41.0	35.0	41.0
20	39.17715758118143	41.0	39.0	41.0	35.0	41.0
21	39.21088788393317	41.0	39.0	41.0	35.0	41.0
22	39.207336499764835	41.0	39.0	41.0	35.0	41.0
23	39.16408654312043	41.0	39.0	41.0	35.0	41.0
24	39.09513523454764	40.0	38.0	41.0	35.0	41.0
25	39.092850830813944	40.0	38.0	41.0	35.0	41.0
26	39.08531780392407	40.0	38.0	41.0	35.0	41.0
27	39.049024818908904	40.0	38.0	41.0	35.0	41.0
28	38.971008244021995	40.0	38.0	41.0	35.0	41.0
29	38.942781316164414	40.0	38.0	41.0	35.0	41.0
30	38.95432806406846	40.0	38.0	41.0	35.0	41.0
31	38.80102804919981	40.0	38.0	41.0	35.0	41.0
32	38.70479481792786	40.0	38.0	41.0	35.0	41.0
33	38.52573805233087	40.0	38.0	41.0	34.0	41.0
34	38.58080669577821	40.0	38.0	41.0	35.0	41.0
35	38.623302856198364	40.0	37.0	41.0	35.0	41.0
36	38.63779155861954	40.0	37.0	41.0	35.0	41.0
37	38.604943242551734	40.0	37.0	41.0	35.0	41.0
38	38.51648685049826	40.0	37.0	41.0	35.0	41.0
39	38.526944500571396	40.0	37.0	41.0	35.0	41.0
40	38.42540169527604	40.0	37.0	41.0	34.0	41.0
41	38.35341634738287	40.0	37.0	41.0	34.0	41.0
42	38.283855918980194	40.0	36.0	41.0	34.0	41.0
43	38.20937463550947	40.0	36.0	41.0	34.0	41.0
44	38.07271481569757	40.0	36.0	41.0	34.0	41.0
45	37.934890189171604	39.0	36.0	41.0	34.0	41.0
46	37.66406860322539	39.0	35.0	41.0	33.0	41.0
47	37.45251556610292	39.0	35.0	41.0	33.0	41.0
48	37.455112124691546	39.0	35.0	41.0	33.0	41.0
49	37.429424219840975	39.0	35.0	40.0	33.0	41.0
50	37.23303453917529	38.0	36.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	11.0
20	52.0
21	177.0
22	366.0
23	828.0
24	1746.0
25	3984.0
26	8040.0
27	17077.0
28	35868.0
29	73297.0
30	139695.0
31	255682.0
32	440840.0
33	792586.0
34	1651815.0
35	1269090.0
36	1800186.0
37	2917186.0
38	4517838.0
39	7797351.0
40	1.3640801E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	50.682623412317675	17.842390683023815	31.474985904658514
2	26.36518956385695	23.817613703858733	30.428507712574422	19.388689019709897
3	26.808113702430745	20.16322517135182	26.79410531249667	26.23455581372076
4	21.288567714518177	23.488080044969365	31.326956188661516	23.89639605185095
5	18.53511037234182	24.7689513453328	30.909749915741823	25.786188366583563
6	21.587401113109944	27.285134160307162	28.734420013775953	22.39304471280694
7	25.36379044975613	23.789322637792832	26.649567797058527	24.197319115392517
8	21.29185914889106	25.128467030558678	27.343608018895548	26.23606580165471
9	21.80910919166698	26.54881710401224	28.3434632127493	23.29861049157148
10	25.090191381356476	23.813686038614804	28.413242186993003	22.68288039303572
11	24.145238670134965	23.846719145439575	27.856953017969623	24.15108916645584
12	22.166405138076094	25.551011987409645	28.18541498130287	24.09716789321139
13	23.302357184478762	25.908932853918763	26.616831597973572	24.171878363628903
14	20.565905586562305	25.62013776462574	27.456509307732457	26.3574473410795
15	21.8795696398659	29.436100661451064	26.66658485208947	22.01774484659357
16	22.39113036480828	30.439397157393987	25.069014790275258	22.100457687522475
17	23.624315454463527	32.50424627552569	22.36451329285642	21.506924977154362
18	22.865512585339413	28.065976066804353	24.667049781333105	24.40146156652313
19	25.466627980059446	25.998669683664033	24.963589921096343	23.571112415180174
20	24.595588329897076	25.45192963309188	27.16738903377201	22.785093003239037
21	24.345993545338843	27.803829154083555	25.455622599836715	22.394554700740894
22	25.795505614228453	28.08341444805657	24.49113053164215	21.629949406072825
23	27.707789397175773	26.86947746302007	24.55799132695732	20.864741812846834
24	23.859845295232475	28.52618306352856	24.720985193041354	22.892986448197618
25	23.898229462249006	27.358914376429244	26.353709785009478	22.389146376312276
26	24.57588830492041	29.67285644135712	26.318742729668422	19.432512524054047
27	26.652051199415656	27.80056656375123	24.266466655906886	21.280915580926234
28	25.428934857467333	27.192803050780483	24.784911392806485	22.593350698945695
29	25.546786050427635	28.380370119666527	24.301468233289995	21.771375596615844
30	27.291998597728373	28.91162696791463	24.503339904403852	19.293034529953147
31	24.7349603713951	27.945135491604216	25.133557950980716	22.18634618601996
32	24.037021246033877	27.8943160773143	26.83429550869556	21.234367167956254
33	23.213143730544164	26.164154160793863	28.453991667965216	22.168710440696763
34	23.349225413730025	27.279112867980515	27.424005664882618	21.947656053406845
35	21.58522958061745	26.580494786379738	27.139287579593656	24.69498805340915
36	23.098264276170603	25.92139544666054	28.431692243355755	22.548648033813105
37	21.890366474693042	28.724998438035072	26.0393425477922	23.345292539479683
38	22.20166364877329	26.75405311791851	26.618340093474398	24.4259431398338
39	22.10258393710638	28.17763578608357	27.57344424944515	22.1463360273649
40	20.18663188025296	27.61017450181586	28.84636397918781	23.356829638743367
41	21.581800217062124	28.487676142882286	26.327027298753297	23.603496341302293
42	22.22126820215986	29.62825268865008	25.530469688941377	22.62000942024869
43	20.774817840636402	26.91252882790549	26.356915018962297	25.955738312495807
44	20.20238527910834	29.03243172330337	25.857340459873733	24.907842537714558
45	20.345446961064862	27.075302013270345	28.45784222218317	24.121408803481618
46	20.857292792675157	28.75594441318259	27.123228343222838	23.263534450919416
47	22.211606018961874	28.067129828551156	28.76406827154901	20.95719588093796
48	21.864429879249844	28.487418943401625	26.03565995843044	23.61249121891809
49	19.871706228923568	30.108999492280674	25.35056520077108	24.66872907802468
50	19.913561351484184	31.373782420677987	27.60493529883264	21.10772092900519
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	265077.0
1	254557.5
2	209607.5
3	171740.0
4	158159.0
5	111270.5
6	58136.0
7	44146.0
8	48074.0
9	50103.5
10	49621.0
11	47796.0
12	45517.5
13	46517.0
14	59158.0
15	75872.5
16	86495.0
17	97151.0
18	112513.0
19	133515.5
20	156415.5
21	190482.5
22	228124.5
23	264494.0
24	295407.5
25	325449.5
26	369062.0
27	435102.5
28	511858.0
29	594182.0
30	713387.0
31	907173.0
32	1152927.0
33	1246212.0
34	1274300.5
35	1405291.5
36	1480389.5
37	1490038.0
38	1566435.5
39	1778803.0
40	2011547.0
41	2169489.5
42	2339697.0
43	2514746.0
44	2644023.0
45	2765746.0
46	2915761.5
47	2942116.5
48	3008776.5
49	3283369.0
50	3583817.5
51	3600815.5
52	3305355.0
53	3002669.0
54	2684537.5
55	2374796.0
56	2181345.5
57	2019424.5
58	1800967.0
59	1554047.0
60	1313151.5
61	1073489.0
62	863930.5
63	695023.0
64	584532.5
65	521292.0
66	379823.0
67	235199.5
68	157023.0
69	103911.0
70	74639.0
71	54251.0
72	41143.5
73	31832.5
74	23745.0
75	18158.5
76	14407.0
77	9646.0
78	6044.5
79	4373.5
80	3328.5
81	2579.0
82	1987.5
83	1166.5
84	619.5
85	498.0
86	363.0
87	285.5
88	220.5
89	175.0
90	181.0
91	169.0
92	143.5
93	143.0
94	72.5
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	142736.0
25	113345.0
26	126322.0
27	169256.0
28	154882.0
29	165376.0
30	381007.0
31	597436.0
32	567107.0
33	110363.0
34	105596.0
35	114155.0
36	141819.0
37	200400.0
38	95687.0
39	129740.0
40	131243.0
41	146066.0
42	195376.0
43	369695.0
44	1274620.0
45	2783483.0
46	3875163.0
47	4365101.0
48	6202708.0
49	6642579.0
50	6063260.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.933824771347451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.68346758608142	6.9222335646000275
2	12.544446936707326	2.4922867544543377
3	5.059020877528104	1.5076628073585778
4	2.594176999797237	1.0308039896738241
5	1.5325536204945498	0.7612059559343489
6	1.0518395378643817	0.626927379403191
7	0.7618676724589606	0.5297781970013153
8	0.6253271045394094	0.49695119050148495
9	0.4740449574603841	0.4238171587037081
>10	3.76292400528751	7.382086057587643
>50	0.44686191757259996	3.129751048288644
>100	1.1701573542009311	32.50401641650929
>500	0.21478716184407493	13.566952320840764
>1k	0.06833513302103382	12.78804293574243
>5k	0.0055021329768376	3.836404108573576
>10k+	0.004687002165454252	12.001080114826973
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	114882	0.3248509996784631	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	106463	0.30104465433025374	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	100263	0.2835129592169508	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	62338	0.17627271128598065	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG	61971	0.17523494804298353	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	60722	0.17170315978548106	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	59430	0.16804978073928953	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	57447	0.1624424659957928	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	52269	0.1478006728834246	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	51235	0.1448768385693673	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	41320	0.1168402648518836	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	38683	0.10938363904320944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.044598370214034566	0.0	0.0	0.0	0.0
2	0.07940161270670117	0.0	0.0	0.0	0.0
3	0.10651635858435633	0.0	0.0	0.0	0.0
4	0.1639807308573471	0.0	0.0	0.0	0.0
5	0.19422856031331515	0.0	0.0	0.0	0.0
6	0.2746877301123349	0.0	0.0	0.0	0.0
7	0.30942593567151666	0.0	0.0	0.0	0.0
8	0.36039792536706494	0.0	0.0	0.0	0.0
9	0.4093452870463027	0.0	0.0	0.0	0.0
10	0.44652661915030606	0.0	0.0	0.0	0.0
11	0.4919026048733984	0.0	0.0	0.0	0.0
12	0.5342246824154638	0.0	0.0	0.0	0.0
13	0.5803726282620936	0.0	0.0	0.0	0.0
14	0.6234638382349361	0.0	0.0	2.8276927602101553E-6	0.0
15	0.6755358004142061	0.0	0.0	2.8276927602101553E-6	0.0
16	0.719291518185698	0.0	0.0	2.8276927602101553E-6	0.0
17	0.7784383676510138	0.0	0.0	2.8276927602101553E-6	0.0
18	0.8172654169414595	0.0	0.0	2.8276927602101553E-6	0.0
19	0.8629185165550525	0.0	0.0	2.8276927602101553E-6	0.0
20	0.9090806008654833	0.0	0.0	2.8276927602101553E-6	0.0
21	0.9444663480667531	0.0	0.0	2.8276927602101553E-6	0.0
22	0.9807032307888462	0.0	0.0	2.8276927602101553E-6	0.0
23	1.0153820548000636	0.0	0.0	2.8276927602101553E-6	0.0
24	1.0506066235140015	0.0	0.0	2.8276927602101553E-6	0.0
25	1.087278970921167	0.0	0.0	2.8276927602101553E-6	0.0
26	1.123541302878102	0.0	0.0	2.8276927602101553E-6	0.0
27	1.154920209438154	0.0	0.0	2.8276927602101553E-6	0.0
28	1.1890193564335283	0.0	0.0	2.8276927602101553E-6	0.0
29	1.2244842790320842	0.0	0.0	2.8276927602101553E-6	0.0
30	1.258673912195785	0.0	0.0	2.8276927602101553E-6	0.0
31	1.2899481941237094	0.0	0.0	2.8276927602101553E-6	0.0
32	1.3207983221376023	0.0	0.0	2.8276927602101553E-6	0.0
33	1.3528813241949467	0.0	0.0	2.8276927602101553E-6	0.0
34	1.385029363185776	0.0	0.0	2.8276927602101553E-6	0.0
35	1.415005734136764	0.0	0.0	2.8276927602101553E-6	0.0
36	1.4402853074130426	0.0	0.0	2.8276927602101553E-6	0.0
37	1.457163805498737	0.0	0.0	2.8276927602101553E-6	0.0
38	1.4717829770690234	0.0	0.0	2.8276927602101553E-6	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	35025	0.0	138.34914	44
ACTTCTA	29125	0.0	97.64277	44
ACCACTC	5440	0.0	79.331055	44
CCCATAC	13035	0.0	76.02212	44
AACGACA	8670	0.0	74.1196	43
TCCGGGC	5820	0.0	71.85148	43
CCTATCA	34210	0.0	67.94073	43
CCTTAGC	34875	0.0	66.51716	43
AATCGCA	1840	0.0	66.284164	44
TATCGCC	495	0.0	63.966537	44
CGGTCCG	35	1.254605E-4	63.80707	43
CCCTATC	39015	0.0	61.307297	42
ACCTTAG	27555	0.0	57.59316	42
GAACACA	20435	0.0	57.4452	44
TCGTGCG	1965	0.0	56.825638	43
TCCAGAA	24535	0.0	55.046116	43
CAATGAA	30005	0.0	52.640038	43
ATACGTC	580	0.0	48.526344	44
AACCTTA	42070	0.0	47.983448	41
GACGGGC	1170	0.0	47.718967	43
>>END_MODULE