FastQCFastQC Report
Tue 24 May 2016
ERR841068.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841068.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9805335
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA477690.4871735641872511No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC346110.3529813106844386No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT250590.2555649552004087No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA187450.19117143881366622No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC179240.18279844594804767No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG167950.1712843059416124No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG123500.12595184152300762No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG108120.11026650287828005No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC100390.10238303943720434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGG1550.0145.9740644
ATATGCG1150.0145.73944
ACGTAAT202.1727568E-4125.6998844
GCGTATT1100.0114.2726144
TCGGTGC307.79087E-6111.7332344
ATTCGAC453.9926817E-9111.7332244
TACGGTT253.1005948E-7101.5884643
TCCGCAA1900.0101.58845543
CATACGC1050.096.75091643
ACGGTTA455.2093856E-793.1110244
ATACCGG1400.090.7039843
CTATCAT124850.089.8743244
CGACGAT1500.084.6570543
CGCGTAT900.084.6570443
CGGTAAA1400.083.7999344
GATTACG1650.080.039443
ACGTTCG2200.079.9908344
TGTCTCG2650.075.89426444
AACGAAC1900.074.97887444
CGGTATG700.072.5631943
Produced by FastQC (version 0.11.5)