##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841068.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9805335
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.78291613698053	39.0	38.0	39.0	35.0	39.0
2	38.10398431058194	39.0	38.0	39.0	35.0	39.0
3	38.20595196390536	39.0	38.0	39.0	35.0	39.0
4	38.63501828341408	39.0	38.0	40.0	35.0	41.0
5	39.43791150429843	41.0	39.0	41.0	36.0	41.0
6	39.4638753290938	41.0	39.0	41.0	36.0	41.0
7	39.47214980416273	41.0	39.0	41.0	36.0	41.0
8	39.4019573018158	41.0	39.0	41.0	36.0	41.0
9	39.38859947161418	40.0	39.0	41.0	36.0	41.0
10	39.37304385826695	40.0	39.0	41.0	36.0	41.0
11	39.318538836255975	40.0	39.0	41.0	36.0	41.0
12	39.252026575328635	40.0	39.0	41.0	35.0	41.0
13	39.191937348392486	40.0	39.0	41.0	35.0	41.0
14	39.15874215414364	40.0	39.0	41.0	35.0	41.0
15	39.167950100633995	40.0	39.0	41.0	35.0	41.0
16	39.22842799353617	40.0	39.0	41.0	35.0	41.0
17	39.26565252487549	40.0	39.0	41.0	35.0	41.0
18	39.23323976182354	41.0	39.0	41.0	35.0	41.0
19	39.20770162365692	41.0	39.0	41.0	35.0	41.0
20	39.24792054529498	41.0	39.0	41.0	35.0	41.0
21	39.303547609541134	41.0	39.0	41.0	35.0	41.0
22	39.31128472408133	41.0	39.0	41.0	35.0	41.0
23	39.2634100721699	41.0	39.0	41.0	35.0	41.0
24	39.20870648478609	41.0	39.0	41.0	35.0	41.0
25	39.20916278800918	41.0	39.0	41.0	35.0	41.0
26	39.20301458969772	41.0	39.0	41.0	35.0	41.0
27	39.162434801229004	41.0	39.0	41.0	35.0	41.0
28	39.123739964853414	40.0	39.0	41.0	35.0	41.0
29	39.10287472591875	40.0	38.0	41.0	35.0	41.0
30	39.12725594073553	40.0	39.0	41.0	35.0	41.0
31	39.04862376034052	40.0	38.0	41.0	35.0	41.0
32	38.95359875413855	40.0	38.0	41.0	35.0	41.0
33	38.76474048250429	40.0	38.0	41.0	35.0	41.0
34	38.79390726051274	40.0	38.0	41.0	35.0	41.0
35	38.83087079359475	40.0	38.0	41.0	35.0	41.0
36	38.870877607551925	40.0	38.0	41.0	35.0	41.0
37	38.79213391961282	40.0	38.0	41.0	35.0	41.0
38	38.747742141749946	40.0	38.0	41.0	35.0	41.0
39	38.74954184849991	40.0	38.0	41.0	35.0	41.0
40	38.66878582802902	40.0	37.0	41.0	35.0	41.0
41	38.62177864390708	40.0	37.0	41.0	35.0	41.0
42	38.56559325493292	40.0	37.0	41.0	35.0	41.0
43	38.504733282974584	40.0	37.0	41.0	35.0	41.0
44	38.42079680481554	40.0	37.0	41.0	34.0	41.0
45	38.23538820594918	40.0	37.0	41.0	34.0	41.0
46	37.95569194382763	40.0	36.0	41.0	34.0	41.0
47	37.75562575258785	39.0	36.0	41.0	33.0	41.0
48	37.654336589546915	39.0	36.0	41.0	33.0	41.0
49	37.79779969447303	39.0	36.0	41.0	33.0	41.0
50	37.21773495907597	38.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	1.0
20	9.0
21	25.0
22	62.0
23	141.0
24	371.0
25	756.0
26	1619.0
27	3617.0
28	8121.0
29	16883.0
30	33531.0
31	62347.0
32	107502.0
33	187108.0
34	386931.0
35	345717.0
36	476650.0
37	736074.0
38	1160966.0
39	2158264.0
40	4118637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	57.0769076222281	14.462647120164688	28.46044525760721
2	26.052205253568594	22.540382353076158	31.968494702118793	19.438917691236455
3	24.32141278191923	22.579901655578315	26.57815362759151	26.520531934910945
4	20.55593205127617	24.49116730840915	30.46449713344827	24.488403506866415
5	16.944306339355055	28.35282017391553	28.892057232108847	25.810816254620573
6	19.500710582555314	28.986414028689484	27.184741775778186	24.32813361297702
7	23.241816827268014	27.386876633995676	24.55091029526273	24.820396243473578
8	20.88108157446941	27.26529996170452	27.371660427716137	24.481958036109937
9	22.112421452199236	27.770300555768873	27.426253157082343	22.691024834949545
10	25.554037674388486	24.428680917072185	27.42400948055319	22.59327192798614
11	23.910768984435514	25.03327015344198	26.66802307111384	24.38793779100867
12	22.96183659201853	24.919107812226713	28.323132254022937	23.79592334173182
13	23.31753071159731	25.29592308676858	26.69899600574585	24.687550195888257
14	18.10495000935715	28.542757590638157	26.112937497800942	27.23935490220375
15	19.86741911418631	31.016349772853246	27.273734145748207	21.842496967212238
16	21.503181686296287	31.787348417978578	23.943659242646987	22.765810653078145
17	21.773922053657525	35.76943572045218	21.962299095339425	20.494343130550867
18	21.44499907448343	29.009279132227505	25.048343580306025	24.497378212983033
19	22.969975018701554	27.391669942944326	24.990028387607357	24.648326650746764
20	22.76302645447606	25.36175459583992	27.24846218920618	24.626756760477843
21	21.058219836446177	29.406960598490517	25.46864538539479	24.06617417966852
22	24.674710247023686	28.269906127633583	23.429694140995693	23.625689484347042
23	24.874020112520377	27.83841653548808	26.635673334975294	20.651890017016246
24	22.83831200055888	28.603194077509848	25.714623722697898	22.843870199233375
25	22.34623354607763	27.564325865542944	25.785499115313137	24.303941473066285
26	22.473110708151232	31.585271896299393	26.650550780642952	19.29106661490642
27	23.982894025005237	28.532068298302427	25.058050262699638	22.4269874139927
28	23.431416874599226	27.102690351946418	25.053864393695523	24.412028379758834
29	23.859323411153678	30.14786353334896	25.071978916691233	20.92083413880613
30	23.08129070059272	30.11091928917558	25.699641020602897	21.1081489896288
31	23.814766767286454	28.423209630432833	25.863684658307516	21.898338943973197
32	22.670522600196865	29.538992068092096	25.43329194520578	22.357193386505255
33	21.599007074075715	26.865071501270275	28.861918196601767	22.674003228052246
34	22.590542486431573	29.328167937849564	26.74695630088501	21.33433327483385
35	20.418318930060273	28.048400681912216	26.82103521008592	24.712245177941593
36	22.45832214965256	25.558241100860137	28.73331000110521	23.250126748382094
37	20.012579491728633	29.806700429270748	26.618288184879766	23.56243189412085
38	22.62855417143892	28.32654239654569	25.87191930848241	23.17298412353298
39	21.40461385118893	29.68942717658456	27.613572673667697	21.292386298558814
40	20.097558280557585	26.911533673557592	30.00520851433923	22.9856995315456
41	19.4231060258055	30.60536134334687	26.72157169384535	23.24996093700228
42	21.61543508256684	29.910819128976485	25.738149864123134	22.735595924333545
43	20.066936772703077	25.5560859048603	27.07874469364096	27.298232628795667
44	20.153580501181978	27.478677434863126	25.021241490744718	27.346500573210175
45	19.106191239193258	27.37751814499471	30.23491876808379	23.28137184772824
46	20.327207878978097	30.342637582365228	27.25006809082437	22.080086447832308
47	21.75008814638046	29.43454188793894	27.81438275561385	21.00098721006675
48	22.023985386884597	29.070119081300245	25.81639367994591	23.08950185186925
49	20.576026998868656	29.629136279090577	24.70179161526612	25.093045106774646
50	19.72479973481194	31.380897495850096	26.988466517893006	21.905836251444963
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44159.0
1	44770.5
2	43892.5
3	42012.5
4	40152.5
5	30135.5
6	17523.0
7	13847.0
8	14368.5
9	15666.0
10	17522.5
11	18027.0
12	16932.0
13	15923.0
14	18153.5
15	20984.0
16	21567.5
17	22740.5
18	25406.0
19	29794.5
20	35206.5
21	46616.0
22	60078.0
23	71656.0
24	81223.5
25	87090.0
26	96488.5
27	122449.5
28	154978.5
29	183805.0
30	224513.0
31	289661.5
32	369751.0
33	407086.5
34	427381.5
35	472532.0
36	498148.0
37	505658.0
38	523080.5
39	571914.0
40	629945.5
41	675738.0
42	727420.0
43	769308.0
44	787634.0
45	811859.5
46	852983.0
47	844901.0
48	850647.0
49	908189.0
50	978173.5
51	986855.5
52	887999.0
53	755617.0
54	629465.5
55	534249.0
56	481135.0
57	444842.0
58	389094.5
59	334784.0
60	288379.0
61	233918.5
62	182605.5
63	141659.5
64	115257.0
65	99328.5
66	68017.0
67	39525.5
68	27586.0
69	19833.0
70	13081.0
71	8261.0
72	6037.5
73	4757.5
74	3547.5
75	2583.5
76	2003.5
77	1445.5
78	906.0
79	535.0
80	281.0
81	122.0
82	60.0
83	35.5
84	25.5
85	52.0
86	76.5
87	69.5
88	63.5
89	63.0
90	33.5
91	3.5
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	30690.0
25	32291.0
26	28789.0
27	36950.0
28	39418.0
29	38596.0
30	75623.0
31	133812.0
32	184854.0
33	30442.0
34	26065.0
35	27373.0
36	38984.0
37	48330.0
38	24635.0
39	30875.0
40	30522.0
41	34973.0
42	43744.0
43	80144.0
44	231182.0
45	652160.0
46	1100860.0
47	1056857.0
48	1835891.0
49	1546161.0
50	2365114.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.846326140164772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.01438943642692	7.593989023319938
2	12.322389434351704	2.673053092621982
3	5.057192664643	1.645558829831007
4	2.6870320976867603	1.1657770592240677
5	1.538278234360511	0.8342333712095463
6	1.0344673210173616	0.6732101967058098
7	0.7724297956808774	0.5864617839034945
8	0.5667172086448963	0.49174397393650815
9	0.46808494573034815	0.4569301784423416
>10	3.4390926831282567	7.279562665631703
>50	0.462926084778383	3.5891601400041586
>100	1.3103139438270885	39.53158913168301
>500	0.2823861641251265	19.389586586921816
>1k	0.0398909822927688	7.789915415081608
>5k	0.0025194304605959243	1.8790656610302061
>10k+	0.001889572845446943	4.420162890452847
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	47769	0.4871735641872511	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	34611	0.3529813106844386	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	25059	0.2555649552004087	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	18745	0.19117143881366622	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	17924	0.18279844594804767	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	16795	0.1712843059416124	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	12350	0.12595184152300762	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG	10812	0.11026650287828005	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	10039	0.10238303943720434	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.031595044942370656	0.0	0.0	0.0	0.0
2	0.050778479266644125	0.0	0.0	0.0	0.0
3	0.07061461949030808	0.0	0.0	0.0	0.0
4	0.10638086307097105	0.0	0.0	0.0	0.0
5	0.13270326816982794	0.0	0.0	0.0	0.0
6	0.19277260797310852	0.0	0.0	0.0	0.0
7	0.22177722637727318	0.0	0.0	0.0	0.0
8	0.2588692788160731	0.0	0.0	0.0	0.0
9	0.3001325298931653	0.0	0.0	0.0	0.0
10	0.33135022923745083	0.0	0.0	0.0	0.0
11	0.36772838459879237	0.0	0.0	0.0	0.0
12	0.40037387809799463	0.0	0.0	0.0	0.0
13	0.4353140407747415	0.0	0.0	0.0	0.0
14	0.46966268873016576	0.0	0.0	0.0	0.0
15	0.5100896603736639	0.0	0.0	0.0	0.0
16	0.5467227789769549	0.0	0.0	0.0	0.0
17	0.5893322359715399	0.0	0.0	0.0	0.0
18	0.6237012809863202	0.0	0.0	0.0	0.0
19	0.6606403554799505	0.0	0.0	0.0	0.0
20	0.697895584393598	0.0	0.0	0.0	0.0
21	0.7292560631533752	0.0	0.0	0.0	0.0
22	0.7600046301324738	0.0	0.0	0.0	0.0
23	0.7898047338515206	0.0	0.0	0.0	0.0
24	0.8206042934790091	0.0	0.0	0.0	0.0
25	0.8525970810788208	0.0	0.0	0.0	0.0
26	0.8868131481484315	0.0	0.0	0.0	0.0
27	0.915878957730664	0.0	0.0	0.0	0.0
28	0.9482796865175948	0.0	0.0	0.0	0.0
29	0.980884385898085	0.0	0.0	0.0	0.0
30	1.0120306955346248	0.0	0.0	0.0	0.0
31	1.041198490413637	0.0	0.0	0.0	0.0
32	1.071814476506922	0.0	0.0	0.0	0.0
33	1.1018389478788844	0.0	0.0	0.0	0.0
34	1.1349025810948836	0.0	0.0	0.0	0.0
35	1.1672013245850346	0.0	0.0	0.0	0.0
36	1.1931055899670944	0.0	0.0	0.0	0.0
37	1.2095762153970262	0.0	0.0	0.0	0.0
38	1.2244150760784818	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCGG	155	0.0	145.97406	44
ATATGCG	115	0.0	145.739	44
ACGTAAT	20	2.1727568E-4	125.69988	44
GCGTATT	110	0.0	114.27261	44
TCGGTGC	30	7.79087E-6	111.73323	44
ATTCGAC	45	3.9926817E-9	111.73322	44
TACGGTT	25	3.1005948E-7	101.58846	43
TCCGCAA	190	0.0	101.588455	43
CATACGC	105	0.0	96.750916	43
ACGGTTA	45	5.2093856E-7	93.11102	44
ATACCGG	140	0.0	90.70398	43
CTATCAT	12485	0.0	89.87432	44
CGACGAT	150	0.0	84.65705	43
CGCGTAT	90	0.0	84.65704	43
CGGTAAA	140	0.0	83.79993	44
GATTACG	165	0.0	80.0394	43
ACGTTCG	220	0.0	79.99083	44
TGTCTCG	265	0.0	75.894264	44
AACGAAC	190	0.0	74.978874	44
CGGTATG	70	0.0	72.56319	43
>>END_MODULE