FastQCFastQC Report
Tue 24 May 2016
ERR841067.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841067.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056049
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT233051.1334846591691152No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC231131.12414636032507No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC125540.6105885608757379No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA112800.5486250570876473No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG111210.5408917783574224No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA86460.4205152698209041No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT78000.3793683905393305No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA74780.36370728518629664No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA72650.35334760990618413No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA72280.3515480418997796No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA62810.3054888283304532No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT59890.29128683217180135No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG58660.285304484474835No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA54340.2642933120757336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA52550.2555872938825874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA50750.24683263871629518No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG46250.22494600080056457No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA41690.20276754104595757No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA41200.20038432936180023No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC40960.1992170420062946No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC40500.1969797412415755No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA39900.1940615228528114No Hit
CGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAAT37780.18375048454584497No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC37060.18024862247932807No Hit
ATAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGG36550.1777681368488786No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC35710.1736826311046089No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC35390.17212624796393472No Hit
AAAAAAAAAAAAAAAAAAAAAAAA33650.16366341463651887No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG32580.1584592585098896No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT31800.1546655746044963No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAG31520.15330373935640637No Hit
CGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGA28510.1386640104394399No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27080.1317089232795522No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC25790.12543475374370944No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG25360.1233433638984285No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT22500.10943318957865303No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT22470.10928727865921484No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA22310.10850908708887774No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG21730.1056881426464058No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGAC21700.10554223172696758No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT21690.10549359475382153No Hit
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGG20920.10174854782157429No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA20740.10087308230494506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTA750.0197.4990444
CCGGATG450.0197.4990444
GTTAGTC1200.0197.4990444
TCATACG400.0197.4990444
TACAGTT750.0197.4990444
GCCAGCA700.0197.4990244
GTAAAGT1350.0190.1842744
TTAGAAC2250.0188.721344
AGTATAC950.0187.1043544
AGCGCCT1500.0184.3324444
CTAGAGA1000.0177.7491344
ATGACGC500.0177.7491344
AGATCGT800.0172.8116644
TATGGTA403.6379788E-12172.8116644
TCTCACG1200.0172.8116644
GCATAGA750.0171.1658344
CGGATTT352.2009772E-10169.2848844
TTCTCGC1350.0168.2399344
TCCATTG900.0164.5825344
ATCCAAT1750.0157.9992244
Produced by FastQC (version 0.11.5)