##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841067.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2056049
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.75319265250974	39.0	37.0	39.0	32.0	39.0
2	37.537985719211946	39.0	37.0	39.0	35.0	39.0
3	37.61323684406354	39.0	37.0	39.0	35.0	39.0
4	38.08883445871183	39.0	37.0	40.0	35.0	41.0
5	38.75716872506443	40.0	38.0	41.0	35.0	41.0
6	38.6663941374938	40.0	38.0	41.0	35.0	41.0
7	38.67977611428521	40.0	38.0	41.0	35.0	41.0
8	38.53985921541753	40.0	38.0	41.0	35.0	41.0
9	38.567103702295036	40.0	38.0	41.0	35.0	41.0
10	38.511859882716806	40.0	38.0	41.0	35.0	41.0
11	38.390358887361145	40.0	38.0	41.0	34.0	41.0
12	38.442422335265356	40.0	38.0	41.0	35.0	41.0
13	38.29356450162423	40.0	37.0	41.0	34.0	41.0
14	38.30650388196001	40.0	37.0	41.0	34.0	41.0
15	38.18557048008097	40.0	37.0	41.0	34.0	41.0
16	38.34820424999599	40.0	37.0	41.0	34.0	41.0
17	38.5081989777481	40.0	38.0	41.0	35.0	41.0
18	38.580467683406376	40.0	38.0	41.0	35.0	41.0
19	38.586420362549724	40.0	38.0	41.0	35.0	41.0
20	38.61679561138864	40.0	38.0	41.0	35.0	41.0
21	38.596862720684186	40.0	38.0	41.0	35.0	41.0
22	38.58640333960913	40.0	38.0	41.0	35.0	41.0
23	38.56565723871367	40.0	38.0	41.0	35.0	41.0
24	38.46383670817184	40.0	37.0	41.0	34.0	41.0
25	38.51364003409886	40.0	38.0	41.0	34.0	41.0
26	38.48411952908376	40.0	37.0	41.0	34.0	41.0
27	38.435312777892634	40.0	37.0	41.0	34.0	41.0
28	38.38938477522589	40.0	37.0	41.0	34.0	41.0
29	38.364044195973214	40.0	37.0	41.0	34.0	41.0
30	38.39761226150225	40.0	37.0	41.0	34.0	41.0
31	38.32691011523688	40.0	37.0	41.0	34.0	41.0
32	38.12548115216226	40.0	36.0	41.0	34.0	41.0
33	38.04680165959799	40.0	36.0	41.0	34.0	41.0
34	38.01687453246833	40.0	36.0	41.0	34.0	41.0
35	38.054197528027075	40.0	36.0	41.0	34.0	41.0
36	38.07285330209225	40.0	36.0	41.0	34.0	41.0
37	38.042073759121884	40.0	36.0	41.0	34.0	41.0
38	37.96055235898355	39.0	36.0	41.0	34.0	41.0
39	37.88296183099984	39.0	35.0	41.0	34.0	41.0
40	37.770075070849344	39.0	35.0	41.0	34.0	41.0
41	37.75408853981113	39.0	35.0	41.0	34.0	41.0
42	37.66066291514878	39.0	35.0	41.0	34.0	41.0
43	37.54271323565356	39.0	35.0	41.0	33.0	41.0
44	37.467975855800915	39.0	35.0	41.0	33.0	41.0
45	37.141264462418334	38.0	35.0	40.0	33.0	41.0
46	36.86347556054643	38.0	35.0	40.0	32.0	41.0
47	36.710838349136	38.0	35.0	40.0	32.0	41.0
48	36.55323398357695	38.0	35.0	40.0	31.0	41.0
49	36.73937971604015	38.0	35.0	40.0	32.0	41.0
50	35.97558806111856	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	8.0
21	34.0
22	62.0
23	138.0
24	262.0
25	489.0
26	905.0
27	1774.0
28	3677.0
29	6868.0
30	12791.0
31	23713.0
32	40987.0
33	71337.0
34	115908.0
35	108904.0
36	170287.0
37	237077.0
38	314649.0
39	473877.0
40	472301.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.64480369874453	18.939626438864053	32.415569862391415
2	26.536089363628978	24.30632732974749	29.385729620257106	19.771853686366427
3	22.870515245502418	24.629325468410528	26.110369937681444	26.389789348405607
4	22.961709570151296	25.088020762151096	29.611988819332613	22.338280848364995
5	15.312475529522887	27.40469706704461	28.81015968004654	28.472667723385968
6	18.482341617344723	33.04157634375445	25.8753074464665	22.600774592434327
7	23.116229233836354	30.52738529091476	20.688563356223515	25.667822119025374
8	21.698315555709033	28.146362270548998	26.40384543364482	23.751476740097146
9	20.605540043063176	30.082065164789363	29.387188729451484	19.925206062695977
10	24.816383267130306	24.162167341342546	27.932019129894275	23.08943026163287
11	21.10115079942161	26.520330984329654	26.671202875028754	25.70731534121998
12	19.38635703721069	29.574878808822163	28.210611712074957	22.82815244189219
13	22.17529835135252	29.27585869792014	25.1337395169084	23.415103433818942
14	19.952199582792044	28.230844692903716	23.28645863984759	28.530497084456645
15	19.38470338012372	30.728304626981167	28.981750921305867	20.90524107158925
16	20.023695933316766	35.20392753285549	23.312284872588155	21.46009166123959
17	21.865237647546337	36.1928631078345	21.564028872852738	20.37787037176643
18	22.60661102921185	29.056943681789686	24.527431009669517	23.809014279328945
19	23.933476293609736	25.691411051001218	22.671103655603538	27.70400899978551
20	22.857431899726123	27.68046870478281	25.743647160160094	23.718452235330968
21	18.39693509250023	33.62522002150727	25.742382598858292	22.235462287134208
22	24.599170545059966	30.891141213074203	21.721369480980268	22.788318760885563
23	26.16688610047718	28.349810729218998	26.686474884596624	18.7968282857072
24	20.29178292929789	31.770984057286572	25.26574026202683	22.67149275138871
25	21.070027803086884	27.90920591099102	26.20690228251221	24.813864003409886
26	20.243883137036313	33.566864610352745	26.01256281283532	20.176689439775622
27	21.821452346637887	31.96589963767005	23.707734354675818	22.504913661016246
28	21.43211109981075	28.730564213624643	23.606117349573942	26.231207336990664
29	22.679517863938376	28.80228590847418	25.667657501818542	22.850538725768903
30	22.62677578298592	31.406522459392942	26.09252258705782	19.874179170563316
31	24.694340588988474	29.973623559539053	24.728604353393084	20.603431498079384
32	21.646563347580052	29.189082257888188	27.337511908418406	21.82684248611335
33	20.23675080009518	24.716647862199736	30.066249311724714	24.98035202598037
34	22.399033124535798	31.243228277912806	23.944649574196777	22.413089023354622
35	18.021716207845817	28.098744239465095	24.87231025841507	29.00722929427402
36	20.261697703909213	27.81781256638193	28.169757516848943	23.75073221285991
37	19.962930893363314	34.75804923018601	22.00119327629731	23.277826600153357
38	22.04992425468395	27.383974899858682	25.40566636664413	25.160434478813233
39	18.326398550701025	32.79408202919234	28.589659625445712	20.289859794660924
40	17.50475056014067	29.051938730318717	29.862751899678642	23.58055880986197
41	16.758820273062607	31.60181749420386	25.03263925809404	26.606722974639492
42	20.594984342952454	31.625747781803433	23.639358196096456	24.139909679147657
43	19.232260544571396	27.295952377778075	24.851180556481395	28.620606521169133
44	20.004759528480378	28.083018328685384	25.186457059882855	26.72576508295138
45	20.122642828354444	26.41857319933057	31.442038891686952	22.016745080628038
46	17.169136596702014	31.46407783381146	27.176541910366286	24.190243659120238
47	19.456697489137863	30.182285158543525	28.689211934348435	21.671805417970173
48	20.38034404386182	31.619217912398433	23.908032357788382	24.092405685951363
49	20.411041898116743	29.26352866264285	23.546314419244467	26.779115019995942
50	19.74341330788725	29.240861107048584	30.38137960301229	20.634345982051876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	50907.0
1	49937.5
2	33927.5
3	18537.0
4	16819.5
5	11190.0
6	4701.0
7	2857.0
8	3509.5
9	3626.5
10	3219.5
11	2738.5
12	2328.5
13	2007.0
14	2164.5
15	2773.5
16	3628.5
17	5347.5
18	7900.5
19	10476.0
20	11656.0
21	11872.0
22	13611.5
23	17959.5
24	23247.0
25	26303.5
26	28532.0
27	29455.0
28	29152.0
29	32800.5
30	40141.0
31	56884.0
32	82622.0
33	94450.0
34	94112.0
35	99458.5
36	107453.5
37	110216.0
38	106727.0
39	102893.5
40	103942.0
41	103558.5
42	107305.5
43	122635.5
44	134808.5
45	148191.0
46	163759.5
47	163122.5
48	162585.0
49	184273.0
50	227288.5
51	255657.0
52	232831.5
53	178739.0
54	139418.0
55	118590.5
56	99922.0
57	92523.5
58	90942.0
59	80721.5
60	62814.0
61	44699.0
62	32906.5
63	24309.5
64	16517.0
65	13592.0
66	10568.0
67	6943.0
68	4922.5
69	3819.0
70	3162.0
71	2461.5
72	1477.0
73	802.5
74	644.5
75	543.0
76	429.5
77	224.5
78	61.0
79	42.5
80	34.0
81	26.0
82	24.5
83	13.0
84	1.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	10233.0
25	11399.0
26	12503.0
27	16078.0
28	16605.0
29	15313.0
30	21418.0
31	31590.0
32	33947.0
33	8748.0
34	7078.0
35	6925.0
36	7140.0
37	9433.0
38	6197.0
39	7974.0
40	8978.0
41	10291.0
42	14031.0
43	22681.0
44	67970.0
45	145775.0
46	235274.0
47	214424.0
48	369643.0
49	327310.0
50	417091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.98374920414461
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.18278437412852	4.761714661091835
2	12.002214361026612	1.676409099835852
3	4.942002421489727	1.0354111643387884
4	2.655246365355166	0.7417429876342801
5	1.7359300484152125	0.6061650047035226
6	1.0827727874969815	0.4537088155770891
7	0.8162662571435638	0.3990419176587524
8	0.6403725675645318	0.35777611272678705
9	0.5139605046556295	0.3230434138815463
>10	4.141275185966	6.010538561970219
>50	0.7782057544589059	3.898647860686454
>100	1.831405892096369	32.38676791677849
>500	0.5521150115878759	26.99780851622777
>1k	0.11410849405089603	12.743997537524034
>5k	0.00779623251279414	3.579489841189136
>10k+	0.0035437420512700636	4.027736588175444
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	23305	1.1334846591691152	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	23113	1.12414636032507	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	12554	0.6105885608757379	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	11280	0.5486250570876473	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	11121	0.5408917783574224	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	8646	0.4205152698209041	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	7800	0.3793683905393305	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	7478	0.36370728518629664	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	7265	0.35334760990618413	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	7228	0.3515480418997796	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	6281	0.3054888283304532	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	5989	0.29128683217180135	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	5866	0.285304484474835	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	5434	0.2642933120757336	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	5255	0.2555872938825874	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	5075	0.24683263871629518	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	4625	0.22494600080056457	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4169	0.20276754104595757	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4120	0.20038432936180023	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	4096	0.1992170420062946	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	4050	0.1969797412415755	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	3990	0.1940615228528114	No Hit
CGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAAT	3778	0.18375048454584497	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	3706	0.18024862247932807	No Hit
ATAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGG	3655	0.1777681368488786	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	3571	0.1736826311046089	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	3539	0.17212624796393472	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	3365	0.16366341463651887	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	3258	0.1584592585098896	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT	3180	0.1546655746044963	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAG	3152	0.15330373935640637	No Hit
CGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGA	2851	0.1386640104394399	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2708	0.1317089232795522	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	2579	0.12543475374370944	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG	2536	0.1233433638984285	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	2250	0.10943318957865303	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	2247	0.10928727865921484	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	2231	0.10850908708887774	No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG	2173	0.1056881426464058	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGAC	2170	0.10554223172696758	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	2169	0.10549359475382153	No Hit
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGG	2092	0.10174854782157429	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA	2074	0.10087308230494506	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.08701154495831569	0.0	0.0	0.0	0.0
2	0.13131982749438365	0.0	0.0	0.0	0.0
3	0.17285580256112573	0.0	0.0	0.0	0.0
4	0.23550022397326134	0.0	0.0	0.0	0.0
5	0.2715402210744977	0.0	0.0	0.0	0.0
6	0.3617618062604539	0.0	0.0	0.0	0.0
7	0.39877454282461167	0.0	0.0	0.0	0.0
8	0.45383159642596066	0.0	0.0	0.0	0.0
9	0.509083197919894	0.0	0.0	0.0	0.0
10	0.5490627898459618	0.0	0.0	0.0	0.0
11	0.592738791731131	0.0	0.0	0.0	0.0
12	0.6344206777173113	0.0	0.0	0.0	0.0
13	0.692882319438885	0.0	0.0	0.0	0.0
14	0.7284359468086606	0.0	0.0	0.0	0.0
15	0.769436915170796	0.0	0.0	0.0	0.0
16	0.8128697321902347	0.0	0.0	0.0	0.0
17	0.8693858949859657	0.0	0.0	0.0	0.0
18	0.9055718030066404	0.0	0.0	0.0	0.0
19	0.9421468068124835	0.0	0.0	0.0	0.0
20	0.9834395970134953	0.0	0.0	0.0	0.0
21	1.0228841822349564	0.0	0.0	0.0	0.0
22	1.0573191592223725	0.0	0.0	0.0	0.0
23	1.0901977530691147	0.0	0.0	0.0	0.0
24	1.1269186677943959	0.0	0.0	0.0	0.0
25	1.1568304062792278	0.0	0.0	0.0	0.0
26	1.1954481629572058	0.0	0.0	0.0	0.0
27	1.2279862979919254	0.0	0.0	0.0	0.0
28	1.2620321791941729	0.0	0.0	0.0	0.0
29	1.2988017308926003	0.0	0.0	0.0	0.0
30	1.3327017011754096	0.0	0.0	0.0	0.0
31	1.3632943572842866	0.0	0.0	0.0	0.0
32	1.3925251781450734	0.0	0.0	0.0	0.0
33	1.423020560307658	0.0	0.0	0.0	0.0
34	1.4548291407451865	0.0	0.0	0.0	0.0
35	1.4805094625663104	0.0	0.0	0.0	0.0
36	1.5046334012467601	0.0	0.0	0.0	0.0
37	1.5227749922302436	0.0	0.0	0.0	0.0
38	1.5364419816842887	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATTA	75	0.0	197.49904	44
CCGGATG	45	0.0	197.49904	44
GTTAGTC	120	0.0	197.49904	44
TCATACG	40	0.0	197.49904	44
TACAGTT	75	0.0	197.49904	44
GCCAGCA	70	0.0	197.49902	44
GTAAAGT	135	0.0	190.18427	44
TTAGAAC	225	0.0	188.7213	44
AGTATAC	95	0.0	187.10435	44
AGCGCCT	150	0.0	184.33244	44
CTAGAGA	100	0.0	177.74913	44
ATGACGC	50	0.0	177.74913	44
AGATCGT	80	0.0	172.81166	44
TATGGTA	40	3.6379788E-12	172.81166	44
TCTCACG	120	0.0	172.81166	44
GCATAGA	75	0.0	171.16583	44
CGGATTT	35	2.2009772E-10	169.28488	44
TTCTCGC	135	0.0	168.23993	44
TCCATTG	90	0.0	164.58253	44
ATCCAAT	175	0.0	157.99922	44
>>END_MODULE