FastQCFastQC Report
Tue 24 May 2016
ERR841066.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841066.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513138
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT150560.9950182997188624No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA123720.8176385762567592No Hit
AGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA102330.6762767176556269Illumina Single End Adapter 1 (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAA101170.6686105299054019No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA99640.6584990926141567No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA91040.6016635627417989No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC82600.5458854380763685No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG78760.5205077131101064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA78430.5183268148708181No Hit
AGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA77620.5129737010107471Illumina Single End Adapter 1 (100% over 26bp)
AGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA77230.5103962758188612Illumina Single End Adapter 1 (100% over 26bp)
AGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA74050.4893803473311753Illumina Single End Adapter 1 (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAA74040.4893142595057424No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT70360.4649939397464078No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC68610.45342857029563727No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA68150.4503885303257205No Hit
AAAAAAAAAAAAAAAAAAAAAAAA55590.36738222158190464No Hit
AGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA53980.3567420816871958Illumina Single End Adapter 1 (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53400.35290898781208324No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG49450.3268042967660584No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA48340.3194685481429982No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG47720.3153711029661538No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC46650.3082997056448255No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA44450.29376038404957117No Hit
AGAGCTCGTATGCCGTCTTCTGCTTG41230.2724801042601534Illumina Single End Adapter 1 (100% over 26bp)
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA39780.2628973695723721No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC37920.25060503404183887No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG37640.2487545749297156No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG37420.24730064277019015No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA36880.24373190019680951No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGGGCT30810.2036165901589941No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCA30490.20150177974513891No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29820.19707389544112963No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA29730.19647910501223287No Hit
AGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA26390.17440577131761942Illumina Single End Adapter 1 (100% over 26bp)
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT26370.17427359566675346No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA26070.17229096090376422No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA24490.1618490844853543No Hit
AGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA24480.1617829966599213Illumina Single End Adapter 1 (100% over 26bp)
ACCATTTGGTGAGCTGAGGGTGAAGCTACAAAGGTTGAAGCGTTCTTCA23410.15471159933859305No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA23230.1535220184807995No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG23030.15220026197214No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT22770.15048197851088269No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA22330.1475741141918318No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC21590.14268361510979172No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC20940.13838790645664836No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA20710.13686788647168996No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG20470.1352817786612986No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC20450.1351496030104326No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT19770.13065563088099036No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19720.13032519175382548No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT19660.12992866480122764No Hit
TCTTTTGGGAGCCATGGAGAGTGACTTTTATCTGCGTTACTACGTGGGG19330.12774776656193948No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA19320.12768167873650652No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG18810.12431119963942483No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG17510.11571978233313816No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA17460.11538934320597329No Hit
TACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT17450.11532325538054031No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC16630.1099040536950364No Hit
TCACATAGACTCAGCTCAGGAGCTCTGCAGCACACAGCTGATCGAACCC16530.10924317544070666No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC16270.10752489197944934No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGGG16100.10640139894708878No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAGG15240.10071784595985296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG400.0204.3353644
TTGTACG350.0204.3353644
TCTAAAG800.0204.3353644
GCGGGCG850.0204.3353644
AATCTGC203.854675E-7204.3353644
GATATAA254.771209E-9204.3353644
ATCCAAT500.0204.3353644
CTCGCGC100.002540478204.3353644
GTAAGGG500.0204.3353644
TTTCTAC100.002540478204.3353644
AAGGATT203.854675E-7204.3353644
TGTAACC153.1316224E-5204.3353644
GCATCTG254.771209E-9204.3353644
CTAATTA350.0204.3353644
AGTAAAC1100.0204.3353644
CTTAAGG1500.0197.5241944
CTATACG750.0190.7130144
CTTAACC500.0183.9018244
CACATAC500.0183.9018244
AGCGCCT500.0183.9018244
Produced by FastQC (version 0.11.5)