FastQCFastQC Report
Tue 24 May 2016
ERR841065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9808676
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA1163241.1859296810293254No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC1132671.1547633951819796No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC671370.6844654671027975No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA618440.6305030362915442No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT602540.6142928974308052No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT349990.35681676099812043No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT349500.35631720325964483No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG300220.30607596784724056No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA221960.2262894604735644No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT179650.18315417901457853No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC177790.18125789861954866No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGA143720.1465233432116628No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT133600.13620594665375838No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA129890.13242358091958586No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC126030.12848828934710455No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA119990.12233047559120108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTA1500.0178.0480344
CTATCAT330100.0118.59762644
GCCGCGC556.184564E-11117.210344
AGGCGCG401.4714715E-7115.1172644
CGACATA154.7276833E-4103.3912843
GTACGTT1100.0103.3912843
AACGACA11750.099.4316243
CCAGAAT31050.099.06386644
GCCGAGT800.096.9293443
ACTTCTA245300.095.5102544
CCCGTTT1850.092.21384443
ACTGCCG1100.092.093844
GAACACA89950.091.0187844
CTTCGCG2350.090.1343644
ATGTACT1350.088.6829244
TTGAGTA1400.085.5156744
GGTACAA3200.083.46001444
ACGCGAC750.082.7130343
AGCGTAC1800.080.4154443
TCCAGAA102650.077.6567743
Produced by FastQC (version 0.11.5)