##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841065.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9808676
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.62170878108319	39.0	38.0	39.0	35.0	39.0
2	37.98700426030995	39.0	38.0	39.0	35.0	39.0
3	38.20452678832495	39.0	38.0	39.0	35.0	39.0
4	38.747709374843254	39.0	39.0	41.0	35.0	41.0
5	39.45655438103981	41.0	39.0	41.0	36.0	41.0
6	39.46985525875256	41.0	39.0	41.0	36.0	41.0
7	39.42866866027586	41.0	39.0	41.0	36.0	41.0
8	39.38740039940151	40.0	39.0	41.0	36.0	41.0
9	39.34291549644417	40.0	39.0	41.0	36.0	41.0
10	39.30266194948228	40.0	39.0	41.0	35.0	41.0
11	39.19383737417772	40.0	39.0	41.0	35.0	41.0
12	39.1738086771344	40.0	39.0	41.0	35.0	41.0
13	39.06195382536848	40.0	38.0	41.0	35.0	41.0
14	39.081318314520736	40.0	38.0	41.0	35.0	41.0
15	39.04978408910642	40.0	38.0	41.0	35.0	41.0
16	39.11404373026492	40.0	38.0	41.0	35.0	41.0
17	39.17452100568925	40.0	38.0	41.0	35.0	41.0
18	39.18881773646107	40.0	39.0	41.0	35.0	41.0
19	39.15962541733461	41.0	39.0	41.0	35.0	41.0
20	39.08614128960932	40.0	38.0	41.0	35.0	41.0
21	39.19729686249194	41.0	39.0	41.0	35.0	41.0
22	39.223915337809096	41.0	39.0	41.0	35.0	41.0
23	39.19553964265921	41.0	39.0	41.0	35.0	41.0
24	39.07630601724433	40.0	38.0	41.0	35.0	41.0
25	39.07311041398047	40.0	38.0	41.0	35.0	41.0
26	39.040892855733105	40.0	38.0	41.0	35.0	41.0
27	38.98575411378876	40.0	38.0	41.0	35.0	41.0
28	38.89433073717033	40.0	38.0	41.0	35.0	41.0
29	38.86618344878692	40.0	38.0	41.0	35.0	41.0
30	38.89051528546535	40.0	38.0	41.0	35.0	41.0
31	38.81407147604966	40.0	38.0	41.0	35.0	41.0
32	38.64112720621753	40.0	37.0	41.0	35.0	41.0
33	38.501146344971616	40.0	37.0	41.0	35.0	41.0
34	38.493624620858235	40.0	37.0	41.0	35.0	41.0
35	38.51092943940459	40.0	37.0	41.0	35.0	41.0
36	38.4886953053652	40.0	37.0	41.0	35.0	41.0
37	38.41398500865782	40.0	37.0	41.0	35.0	41.0
38	38.31191639028013	40.0	36.0	41.0	34.0	41.0
39	38.30741847921436	40.0	36.0	41.0	34.0	41.0
40	38.22040494587604	40.0	36.0	41.0	34.0	41.0
41	38.14953465725107	40.0	36.0	41.0	34.0	41.0
42	38.06565664636799	39.0	36.0	41.0	34.0	41.0
43	38.00880695167323	39.0	35.0	41.0	34.0	41.0
44	37.80176567538127	39.0	35.0	41.0	34.0	41.0
45	37.547976633315656	39.0	35.0	40.0	34.0	41.0
46	37.274568043001054	39.0	35.0	40.0	33.0	41.0
47	37.08223191688937	38.0	35.0	40.0	33.0	41.0
48	36.987771764417694	38.0	35.0	40.0	33.0	41.0
49	37.297776335990946	39.0	35.0	40.0	33.0	41.0
50	36.78699858993816	37.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	35.0
22	109.0
23	230.0
24	450.0
25	988.0
26	1964.0
27	4184.0
28	9274.0
29	19137.0
30	37297.0
31	68811.0
32	121485.0
33	217271.0
34	405178.0
35	379459.0
36	573533.0
37	905587.0
38	1404434.0
39	2146844.0
40	3512394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	44.26738124493051	18.556612533638585	37.17600622143091
2	22.033248931863994	23.250946407038015	33.24430330862187	21.471501352476114
3	23.4895412999675	23.739208023590542	22.51609697374039	30.255153702701566
4	19.954385280949232	27.689496523282042	24.737507896070785	27.61861029969794
5	14.20170265589362	27.993767966237236	27.123222339080215	30.681307038788923
6	18.24958842559383	28.680078738455627	25.24705678931591	27.823276046634632
7	18.16959801710241	26.485062815817344	23.466551448941733	31.87878771813851
8	17.82024403701376	28.02527068892886	27.60987313680256	26.544612137254813
9	18.381808105395674	30.005140347178354	28.193805157801116	23.41924638962486
10	26.386670331449423	22.283272482443095	28.239744079629098	23.090313106478387
11	18.576441917339302	25.350618167018666	24.3376985843961	31.735241331245927
12	17.143006864535028	28.61476921044186	26.394194282694215	27.848029642328893
13	21.83993028212982	27.871325345031277	23.446375433340847	26.84236893949805
14	14.529147460880552	27.079669060329852	23.340938165354835	35.05024531343476
15	19.801408467360936	34.59603518354567	24.8881500418609	20.714406307232494
16	17.46176548190602	34.51005008219254	22.687618593987608	25.340565841913833
17	17.01903498494598	40.88996313059989	19.45230936366947	22.638692520784662
18	17.35445232363675	31.388548260743853	22.59227443132998	28.664724984289418
19	21.439662192940208	26.469892572657105	23.645342144036565	28.445103090366118
20	19.293378637443016	22.571456127208197	30.253440933312508	27.881724302036282
21	16.220374696850012	35.45115569114527	23.65561876037092	24.672850851633797
22	23.029693304172756	26.2832414894732	23.29021776231573	27.39684744403832
23	25.68305854939036	24.156002298373398	28.876996242918008	21.28394290931824
24	17.551879580893488	28.832688530031987	27.110967881903736	26.504464007170796
25	18.199137957740767	23.80606267816288	25.798123186548406	32.19667617754794
26	19.071989753845614	33.14892798112844	27.749919439283055	20.029162825742887
27	21.223954677593508	28.442582715685244	23.43139290584259	26.902069700878656
28	19.530922683815476	24.72617810719858	23.858982073742126	31.883917135243813
29	18.15904959200039	32.393693466396094	24.68082141024999	24.76643553135353
30	22.24730565692547	30.69011693580678	27.396682092666598	19.665895314601155
31	23.189933066629674	29.27338538539211	22.854437182304707	24.682244365673515
32	15.668003995894114	33.40861427887338	25.158913315465565	25.764468409766938
33	16.961201567685414	23.307837514508563	33.507667865881984	26.223293051924042
34	20.038104612122705	30.21549931197887	26.013966169651724	23.7324299062467
35	15.806068555167622	25.211256094461614	25.511075962160636	33.47159938821013
36	17.541881261508134	27.2871395395595	28.07029269631559	27.10068650261677
37	14.604686564250304	36.20538411411958	21.317592731832878	27.872336589797236
38	20.956468747884795	24.816056695006296	23.15260302067287	31.07487153643604
39	17.260103753576235	31.113520695757973	28.378187065260573	23.24818848540522
40	15.031280719459877	25.771552978354634	31.2238555131949	27.97331078899059
41	15.473467568974122	30.74221684105839	21.970005529375953	31.814310060591538
42	15.363486948161547	32.6442415309532	25.905760072720746	26.086511448164508
43	16.066097228548813	24.337527301673724	24.19773958762905	35.39863588214841
44	17.740249855358954	26.143106524530236	22.76441415216533	33.352229467945484
45	15.502465357844756	24.427488862786582	33.856239822225675	26.213805957142988
46	15.313606972846674	31.584497936680805	27.16375169379321	25.938143396679315
47	17.720548761422698	30.068831153508075	29.52952350612652	22.68109657894271
48	18.982940771189945	26.158281807761036	24.00728007902587	30.851497342023155
49	15.605378200443488	29.88271278209113	24.84010963018428	29.671799387281094
50	15.180862108378623	30.98551776997862	30.51492527970554	23.318694841937216
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	74972.0
1	68967.0
2	58785.0
3	52637.0
4	46627.0
5	31001.0
6	14336.5
7	9781.0
8	10895.5
9	11721.0
10	11552.5
11	11733.5
12	12291.5
13	11305.5
14	10939.5
15	12819.0
16	14329.5
17	14860.5
18	17227.5
19	21186.0
20	23925.0
21	23820.5
22	24434.0
23	32151.5
24	41168.0
25	50616.5
26	66449.0
27	88085.5
28	102527.5
29	109468.5
30	145789.0
31	277675.0
32	484678.0
33	534299.5
34	470674.5
35	495750.0
36	498775.0
37	460705.5
38	463871.0
39	496651.5
40	523372.5
41	551611.5
42	607809.5
43	663231.0
44	684302.0
45	719638.0
46	790351.5
47	795642.0
48	845442.5
49	1035480.0
50	1248942.5
51	1331422.0
52	1144791.5
53	835394.0
54	634763.5
55	530728.0
56	470262.0
57	432536.5
58	387372.0
59	341630.5
60	290744.5
61	233779.5
62	181316.5
63	137148.5
64	106920.5
65	87946.0
66	58629.0
67	35461.0
68	25240.5
69	17634.5
70	12505.5
71	8840.5
72	6864.0
73	4608.0
74	3370.5
75	3051.0
76	2320.0
77	2071.0
78	1569.0
79	1059.0
80	815.0
81	348.0
82	52.5
83	39.5
84	27.5
85	18.5
86	13.5
87	18.0
88	25.0
89	25.0
90	20.5
91	8.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	27949.0
25	27214.0
26	29934.0
27	40186.0
28	40124.0
29	40650.0
30	73441.0
31	146547.0
32	177723.0
33	28591.0
34	24155.0
35	25467.0
36	29711.0
37	40525.0
38	22168.0
39	23309.0
40	29860.0
41	32919.0
42	43987.0
43	85834.0
44	269116.0
45	683784.0
46	1085122.0
47	1138663.0
48	1800784.0
49	1684270.0
50	2156643.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.309156674531948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.4165263661911	5.851019500529916
2	12.154585876459876	2.0198871672311665
3	4.840746815113552	1.206675711255601
4	2.5306140041364724	0.8410907297253833
5	1.490434054699752	0.6192125036779078
6	0.9965407506519884	0.49682479378338285
7	0.7166231369874358	0.41681737452762224
8	0.5470674253373072	0.36365351589283956
9	0.44191151689397973	0.33047208271369344
>10	3.5529203156482323	5.991417556673055
>50	0.510149224161046	3.027141211411899
>100	1.4191502544915662	32.061763703503985
>500	0.2824231456793511	15.251914440735323
>1k	0.09014613321499584	14.112466919818873
>5k	0.005992373017181805	3.5100023416671693
>10k+	0.0041686073163003864	13.899640446852166
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	116324	1.1859296810293254	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	113267	1.1547633951819796	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	67137	0.6844654671027975	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	61844	0.6305030362915442	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	60254	0.6142928974308052	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	34999	0.35681676099812043	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	34950	0.35631720325964483	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	30022	0.30607596784724056	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	22196	0.2262894604735644	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT	17965	0.18315417901457853	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	17779	0.18125789861954866	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGA	14372	0.1465233432116628	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	13360	0.13620594665375838	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA	12989	0.13242358091958586	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	12603	0.12848828934710455	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	11999	0.12233047559120108	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03185954964767926	0.0	0.0	0.0	0.0
2	0.05009850462998268	0.0	0.0	0.0	0.0
3	0.07503561133021419	0.0	0.0	0.0	0.0
4	0.10543726798601565	0.0	0.0	0.0	0.0
5	0.13087393242472276	0.0	0.0	0.0	0.0
6	0.18565196770695658	0.0	0.0	0.0	0.0
7	0.21738917668398874	0.0	0.0	0.0	0.0
8	0.2580980348418074	0.0	0.0	0.0	0.0
9	0.29471867558883585	0.0	0.0	0.0	0.0
10	0.3233056122967055	0.0	0.0	0.0	0.0
11	0.35598076641536536	0.0	0.0	0.0	0.0
12	0.3886763106457997	0.0	0.0	0.0	0.0
13	0.4219121928382587	0.0	0.0	0.0	0.0
14	0.4550563195277324	0.0	0.0	0.0	0.0
15	0.4910448668097509	0.0	0.0	0.0	0.0
16	0.5255857161557789	0.0	0.0	0.0	0.0
17	0.5698322587064758	0.0	0.0	0.0	0.0
18	0.6058411961002688	0.0	0.0	0.0	0.0
19	0.6435119276036847	0.0	0.0	0.0	0.0
20	0.6827526977137384	0.0	0.0	0.0	0.0
21	0.7232372646420373	0.0	0.0	0.0	0.0
22	0.7500706517372987	0.0	0.0	0.0	0.0
23	0.7819200063290906	0.0	0.0	0.0	0.0
24	0.8172356799225502	0.0	0.0	0.0	0.0
25	0.8494214713586217	0.0	0.0	0.0	0.0
26	0.8892127744865872	0.0	0.0	0.0	2.0390111774514726E-5
27	0.9153019225020788	0.0	0.0	0.0	2.0390111774514726E-5
28	0.9524730962670191	0.0	0.0	0.0	2.0390111774514726E-5
29	0.9890019815110622	0.0	0.0	0.0	2.0390111774514726E-5
30	1.0235224407453156	0.0	0.0	0.0	2.0390111774514726E-5
31	1.0545867760337888	0.0	0.0	0.0	2.0390111774514726E-5
32	1.0850801881925756	0.0	0.0	0.0	2.0390111774514726E-5
33	1.1140443419682737	0.0	0.0	0.0	2.0390111774514726E-5
34	1.151011614615469	0.0	0.0	0.0	2.0390111774514726E-5
35	1.1795169908762406	0.0	0.0	0.0	2.0390111774514726E-5
36	1.204810924532526	0.0	0.0	0.0	2.0390111774514726E-5
37	1.2230192943471678	0.0	0.0	0.0	2.0390111774514726E-5
38	1.2377205649365928	0.0	0.0	0.0	2.0390111774514726E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGTTA	150	0.0	178.04803	44
CTATCAT	33010	0.0	118.597626	44
GCCGCGC	55	6.184564E-11	117.2103	44
AGGCGCG	40	1.4714715E-7	115.11726	44
CGACATA	15	4.7276833E-4	103.39128	43
GTACGTT	110	0.0	103.39128	43
AACGACA	1175	0.0	99.43162	43
CCAGAAT	3105	0.0	99.063866	44
GCCGAGT	80	0.0	96.92934	43
ACTTCTA	24530	0.0	95.51025	44
CCCGTTT	185	0.0	92.213844	43
ACTGCCG	110	0.0	92.0938	44
GAACACA	8995	0.0	91.01878	44
CTTCGCG	235	0.0	90.13436	44
ATGTACT	135	0.0	88.68292	44
TTGAGTA	140	0.0	85.51567	44
GGTACAA	320	0.0	83.460014	44
ACGCGAC	75	0.0	82.71303	43
AGCGTAC	180	0.0	80.41544	43
TCCAGAA	10265	0.0	77.65677	43
>>END_MODULE