##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841064.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7118565 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.475618189902036 39.0 37.0 39.0 35.0 39.0 2 37.85774618901422 39.0 38.0 39.0 35.0 39.0 3 37.87489501044101 39.0 38.0 39.0 35.0 39.0 4 38.032437015044465 39.0 38.0 39.0 35.0 40.0 5 38.93226584290514 40.0 38.0 41.0 35.0 41.0 6 38.954620067387175 40.0 38.0 41.0 35.0 41.0 7 38.94791478338682 40.0 38.0 41.0 35.0 41.0 8 38.854082248318306 40.0 38.0 41.0 35.0 41.0 9 38.783446242325525 40.0 38.0 41.0 35.0 41.0 10 38.75687979248627 40.0 38.0 41.0 35.0 41.0 11 38.68068409855076 40.0 38.0 41.0 35.0 41.0 12 38.59963377450371 40.0 38.0 41.0 35.0 41.0 13 38.541062981092395 40.0 38.0 41.0 35.0 41.0 14 38.49732453099747 40.0 38.0 41.0 35.0 41.0 15 38.507299575125046 40.0 38.0 41.0 35.0 41.0 16 38.55099068421796 40.0 38.0 41.0 35.0 41.0 17 38.76081457428569 40.0 38.0 41.0 35.0 41.0 18 38.81188933443749 40.0 38.0 41.0 35.0 41.0 19 38.840221589604084 40.0 38.0 41.0 35.0 41.0 20 38.86610236754177 40.0 38.0 41.0 35.0 41.0 21 38.95653716725211 40.0 38.0 41.0 35.0 41.0 22 38.96400974072724 40.0 38.0 41.0 35.0 41.0 23 38.93805113811562 40.0 38.0 41.0 35.0 41.0 24 38.912292154387856 40.0 38.0 41.0 35.0 41.0 25 38.91623953501615 40.0 38.0 41.0 35.0 41.0 26 38.8818782633374 40.0 38.0 41.0 35.0 41.0 27 38.79451108651692 40.0 38.0 41.0 35.0 41.0 28 38.77810685177862 40.0 38.0 41.0 35.0 41.0 29 38.737801066467 40.0 38.0 41.0 35.0 41.0 30 38.72670717994828 40.0 38.0 41.0 35.0 41.0 31 38.64329038844892 40.0 38.0 41.0 35.0 41.0 32 38.55148669202908 40.0 38.0 41.0 34.0 41.0 33 38.32828118073657 40.0 37.0 41.0 34.0 41.0 34 38.328731908305464 40.0 37.0 41.0 34.0 41.0 35 38.39084355875138 40.0 37.0 41.0 34.0 41.0 36 38.419011731725 40.0 37.0 41.0 34.0 41.0 37 38.39167502636813 40.0 37.0 41.0 34.0 41.0 38 38.33296082092625 40.0 37.0 41.0 34.0 41.0 39 38.32975942642158 40.0 37.0 41.0 34.0 41.0 40 38.26225975986459 40.0 36.0 41.0 34.0 41.0 41 38.22007576387337 40.0 36.0 41.0 34.0 41.0 42 38.146449791668566 40.0 36.0 41.0 34.0 41.0 43 38.08327328976684 40.0 36.0 41.0 34.0 41.0 44 37.93704299744097 39.0 36.0 41.0 34.0 41.0 45 37.834935583869495 39.0 36.0 41.0 34.0 41.0 46 37.4862969654545 39.0 35.0 41.0 33.0 41.0 47 37.297821499422774 39.0 35.0 40.0 33.0 41.0 48 37.08545763726146 39.0 35.0 40.0 32.0 41.0 49 37.45046670976259 39.0 35.0 40.0 33.0 41.0 50 36.5721373492619 38.0 35.0 40.0 32.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 8.0 22 29.0 23 75.0 24 192.0 25 594.0 26 1352.0 27 3265.0 28 7392.0 29 15707.0 30 31736.0 31 59592.0 32 102520.0 33 173787.0 34 284675.0 35 315630.0 36 494067.0 37 742140.0 38 1108264.0 39 1899405.0 40 1878134.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 57.71423032591541 11.181115856917792 31.104653817166806 2 25.234889335139876 26.07181363097759 30.648157318223546 18.045139715658983 3 25.439270414753533 20.03058200634538 26.17242379608812 28.357723782812972 4 21.351241437003104 22.619671239919846 30.853381264341902 25.17570605873515 5 16.331507824961914 28.305929636099407 30.25039456688251 25.112167972056167 6 19.88760375159881 33.4202750132927 26.552191347553894 20.139929887554583 7 25.299537196050046 32.05051860873645 23.451397858978602 19.1985463362349 8 23.478608399305198 27.898277251103277 25.647697253589737 22.97541709600179 9 20.685699997120206 28.11032841590967 30.050438536418504 21.15353305055162 10 24.393329273526334 24.85235437198368 26.75435287870519 23.999963475784796 11 21.28014564733201 27.331182618968853 28.321508618661206 23.067163115037932 12 22.546538522862402 26.492066870218927 28.209856902339165 22.751537704579505 13 21.98490847523342 26.159794846292755 27.720390275287226 24.134906403186598 14 16.57255359752984 29.322089494160693 28.979984027679734 25.125372880629733 15 18.833416566400672 30.622337507629698 29.639498972054056 20.90474695391557 16 20.290648466369277 37.60770604749693 22.928047998437886 19.173597487695904 17 20.98467879411089 37.820782699884035 21.49610209361016 19.698436412394916 18 19.060063369513376 29.808353228494788 25.943768161139218 25.18781524085262 19 23.807396575011957 26.163714175539592 26.119632257344 23.909256992104446 20 21.566523590077495 26.149048298357886 29.35527033889555 22.929157772669072 21 20.222629139440322 29.046135000523282 27.39262758716118 23.338608272875224 22 22.87826268356052 27.445784368057325 24.480776673388526 25.195176274993624 23 23.8250124849601 27.063684885928552 27.789182229845483 21.322120399265863 24 21.179549529996567 29.320403761151297 26.68013848296672 22.819908225885413 25 22.60494301873027 28.509663389625818 26.5431428808228 22.342250710821112 26 22.962404741959695 30.226798901600716 27.89625500343656 18.914541353003024 27 23.43933556003667 27.714053313343072 28.186082301109433 20.66052882551082 28 23.109015504163697 27.08328800388745 26.172176807450327 23.635519684498522 29 22.317048479900443 28.738467144300873 26.67826627293114 22.266218102867548 30 23.223729448017526 30.185944153696713 26.175822903341654 20.414503494944103 31 22.60064720324398 29.187530227635012 26.742136197787715 21.469686371333292 32 21.9573021494826 29.004263949022285 27.710956051050665 21.327477850444453 33 20.675195941829983 26.801809253493552 29.54393943045912 22.979055374217346 34 21.462069458764475 29.978174120811936 26.93981520575316 21.61994121467043 35 20.507977581610085 28.651980761762943 27.285361908899407 23.554679747727565 36 20.76625984673352 27.145812383104527 28.827368703668878 23.260559066493077 37 20.260959170535635 30.201580647044686 25.34344534954168 24.194014832878004 38 21.348514230604295 29.152776244281924 26.95819117572602 22.540518349387764 39 21.248640098348698 29.646276487978966 27.87378277812139 21.231300635550944 40 19.733168555883907 27.996786287340957 29.760814402416546 22.50923075435859 41 20.66389756606094 29.893524787360537 27.086169706396518 22.356407940182006 42 21.062693464135187 30.465052365180885 25.66801465573136 22.804239514952577 43 22.03718356885306 28.035303134677562 26.170058274841278 23.757455021628097 44 21.868350103942692 29.466403542263013 24.78808313600828 23.877163217786016 45 20.51983702220227 27.78573867538337 29.49814693323205 22.19627736918231 46 20.20096258191262 31.899118269000432 27.049674061809835 20.850245087277113 47 21.90389199857489 28.482083147542347 29.278078744170966 20.335946109711802 48 21.627656022501544 30.561023367211877 25.66061583856994 22.150704771716644 49 21.355028429003784 30.699986308302957 25.315539122491643 22.629446140201615 50 18.88543119659027 32.93081117707498 27.694269021179725 20.489488605155028 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22639.0 1 22992.5 2 24604.5 3 25854.0 4 25006.5 5 18847.5 6 11192.5 7 9473.0 8 10584.0 9 11465.0 10 11497.0 11 11211.5 12 11303.5 13 11873.5 14 13868.5 15 16526.0 16 18027.0 17 19102.5 18 25047.5 19 35248.5 20 40690.0 21 45488.0 22 53451.5 23 59884.0 24 65862.5 25 76193.5 26 92171.0 27 120865.0 28 150445.5 29 171920.5 30 207358.5 31 275781.5 32 353235.0 33 374731.5 34 361815.5 35 370757.0 36 396465.0 37 419744.0 38 429242.0 39 441235.5 40 467621.5 41 512925.0 42 559788.5 43 585243.0 44 592162.5 45 571064.5 46 558220.5 47 565242.0 48 573089.0 49 604842.5 50 653348.5 51 640282.5 52 588440.5 53 510898.5 54 424189.0 55 380865.0 56 337018.0 57 289242.0 58 242923.5 59 194142.0 60 160077.0 61 133295.5 62 100863.0 63 76808.0 64 64206.5 65 56047.0 66 40044.0 67 23513.0 68 14928.0 69 11476.0 70 8912.0 71 6816.0 72 5328.5 73 4029.0 74 2689.5 75 1911.5 76 1391.5 77 789.0 78 421.0 79 534.5 80 621.0 81 500.5 82 330.0 83 146.5 84 13.0 85 9.5 86 6.0 87 3.5 88 3.0 89 2.5 90 2.0 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 23953.0 25 19225.0 26 18617.0 27 24917.0 28 28167.0 29 25892.0 30 47050.0 31 76769.0 32 174314.0 33 24259.0 34 19243.0 35 21275.0 36 20749.0 37 36859.0 38 17281.0 39 22922.0 40 21101.0 41 22873.0 42 27779.0 43 51893.0 44 137851.0 45 357065.0 46 899614.0 47 557246.0 48 1505565.0 49 769580.0 50 2166506.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.772202810697884 #Duplication Level Percentage of deduplicated Percentage of total 1 71.12655951455868 7.661897243179992 2 11.630314629599955 2.505682158845547 3 4.779079953232833 1.5444365551429393 4 2.4828018958151103 1.0698098224202226 5 1.5155936627074165 0.8163141156646366 6 0.9461913906429377 0.6115539334645196 7 0.7214490551444912 0.5440116877721974 8 0.5501746965554183 0.4741274730087306 9 0.44758608190071114 0.4339339244532084 >10 3.5044689732678678 7.545392673171427 >50 0.49573803486044 3.7764730564589852 >100 1.4996492319862829 44.43719709919892 >500 0.263350212478213 17.56693997968324 >1k 0.032991125519248654 6.46922975349713 >5k 0.002604562540993315 2.0347469906743396 >10k+ 0.0014469791894407306 2.508253533363976 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 21911 0.3078007997398352 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 18593 0.2611902820301564 No Hit CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAATA 16944 0.23802550092609956 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 12130 0.17039951169933829 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG 10881 0.15285384062658697 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 9730 0.13668485151150547 No Hit CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAA 8965 0.12593830357663377 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT 8854 0.12437900054294651 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.022167389073500066 0.0 0.0 0.0 0.0 2 0.033841090163537174 0.0 0.0 0.0 0.0 3 0.04974317155213164 0.0 0.0 0.0 0.0 4 0.07667275637716309 0.0 0.0 0.0 0.0 5 0.09587606490914953 0.0 0.0 0.0 0.0 6 0.14917332355608187 0.0 0.0 0.0 0.0 7 0.1749509908246957 0.0 0.0 0.0 0.0 8 0.20410012411209283 0.0 0.0 0.0 0.0 9 0.2386716985796997 0.0 0.0 0.0 0.0 10 0.2655029489791833 0.0 0.0 0.0 0.0 11 0.2955230443214328 0.0 0.0 0.0 0.0 12 0.32429288768171677 0.0 0.0 0.0 0.0 13 0.3506746092786959 0.0 0.0 0.0 0.0 14 0.38020303249320614 0.0 0.0 0.0 0.0 15 0.4120352908205516 0.0 0.0 0.0 0.0 16 0.4493461814284199 0.0 0.0 0.0 0.0 17 0.49167212773922836 0.0 0.0 0.0 0.0 18 0.5213410287045213 0.0 0.0 0.0 0.0 19 0.5555614087951715 0.0 0.0 0.0 0.0 20 0.5949935134398576 0.0 0.0 0.0 0.0 21 0.6288205558283165 0.0 0.0 0.0 0.0 22 0.6546403664221652 0.0 0.0 0.0 0.0 23 0.6821880533506401 0.0 0.0 0.0 0.0 24 0.7136410217508725 0.0 0.0 0.0 0.0 25 0.7426356295124088 0.0 0.0 0.0 0.0 26 0.774453840064676 0.0 0.0 0.0 0.0 27 0.8012288993638466 0.0 0.0 0.0 0.0 28 0.8277230031614518 0.0 0.0 0.0 0.0 29 0.8574199996769012 0.0 0.0 0.0 0.0 30 0.886021269736246 0.0 0.0 0.0 0.0 31 0.9154232629750518 0.0 0.0 0.0 0.0 32 0.9447971606637012 0.0 0.0 0.0 0.0 33 0.9745643960545419 0.0 0.0 0.0 0.0 34 1.0064668932572787 0.0 0.0 0.0 0.0 35 1.0344781567633363 0.0 0.0 0.0 0.0 36 1.0574041256910627 0.0 0.0 0.0 0.0 37 1.0740226436086486 0.0 0.0 0.0 0.0 38 1.090289967149278 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATCGTC 10 0.009029244 133.74252 44 GCGAGCC 255 0.0 123.25291 44 GCGAATG 50 0.0 120.36826 44 AGCGTCA 610 0.0 119.49126 44 GTATCGC 80 0.0 117.024704 44 TGCACCG 115 0.0 116.297844 44 CGTTAGA 90 0.0 111.452095 44 CGAAGAC 160 0.0 104.48634 44 CGTGCGC 65 0.0 102.87885 44 TTACGAG 150 0.0 102.53593 44 ATGCGTC 110 0.0 94.15104 43 GAGCGTC 590 0.0 91.11145 43 ACGGTCC 65 0.0 91.04716 43 GCGGCAC 105 0.0 89.24067 43 TAGCGGA 30 2.3895804E-5 89.161674 44 ACCACGT 60 1.6370905E-11 89.161674 44 CTATCAT 6245 0.0 88.55489 44 CGTATCG 45 1.8189894E-11 87.67504 43 TCGTGCG 130 0.0 87.253525 43 AACGACA 1060 0.0 85.141975 43 >>END_MODULE