FastQCFastQC Report
Tue 24 May 2016
ERR841063.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841063.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517107
Sequences flagged as poor quality0
Sequence length24-50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT172851.1393395455956632No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC146250.964005834789504No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA120450.7939453182933043No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC92020.6065491755031122No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA91680.6043080679213793No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA79160.5217825769705103No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA66560.4387297665886454No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC64400.4244921419517543No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG59230.3904141237236398No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA55100.36319125809847297No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC48500.3196874050413056No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA47240.3113821240031191No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT44660.2943760723534991No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT37350.24619225934624256No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG34580.22793382404800716No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA33770.22259471480917298No Hit
CCTCTTTTTCCGGCTGGAACCATGGAGGG31790.20954355889202278No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG31450.20730245131028993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA31340.20657738709267043No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA30820.20314981079119668No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA27690.18251843805347942No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA27330.18014550061399756No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT26600.17533371080615937No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA25780.16992868663845068No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA23920.15766850986779443No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC22640.14923139897185894No Hit
TCAAACTTGGAGAAGATAGATTTGTGTCAATCAGTTGAGAGGATTATTGT22020.1451446733816402No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG20250.1334777309708544No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC19930.13136845324687052No Hit
ACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATA18970.12504062007491892No Hit
AATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCAT18440.12154712884457063No Hit
CATTGGACAGACAGAGAGTGAAAGAGAGGGCGAGGGAGAGGGAGAGAA18380.12115163927132365No Hit
AAAAAAAAAAAAAAAAAAAAAAAA18150.11963559590721023No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC17820.11746040325435188No Hit
ACAATCACCAGTCTCAAATGCGCTATTGTTTCTTTTCAGAGTGTTGCAGA17790.11726265846772838No Hit
TGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATA17640.11627393453461095No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17630.11620801960573644No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT17590.11594435989023845No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC17070.11251678358876467No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA16990.1119894641577687No Hit
CCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACAC16800.11073708050915328No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC16620.10955061178941235No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCC15980.10533205634144462No Hit
ATGATGAAGAGGAGGAAGGATTAGAAGATATTGACGAAGAAGGGGATGA15840.10440924733720167No Hit
CTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACC15830.10434333240832716No Hit
AATTACGAAGGCAGTCCAATTAAAGTAACACTGGCAACTTTGAAAATGT15510.1022340546843433No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT15260.10058618146248088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGGT950.0162.4088644
CTCGTTA450.0162.4088644
GCGACCC850.0162.4088644
AACGGTA450.0162.4088644
GGGGATA1100.0162.4088444
ATTACGC100.0050506354162.4088444
TTGCGGA100.0050506354162.4088444
AGCTCGC1250.0162.4088444
ATTGCAC157.825137E-5162.4088444
ACAAGGT100.0050506354162.4088444
TTATTGT2650.0159.3445344
GTGTTAT1350.0156.393744
GCACAGT600.0148.8747744
ACTGGTA550.0147.6444144
ATGACGT1500.0146.1679744
TTCTAAC450.0144.3634344
GGTTTCC900.0144.3634344
CACTTAC750.0140.7543344
TACAAGG1350.0138.3482844
GTCATAA900.0135.3407144
Produced by FastQC (version 0.11.5)