##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841063.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1517107
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.208847497243106	39.0	37.0	39.0	34.0	39.0
2	37.71845360940263	39.0	37.0	39.0	35.0	39.0
3	37.816616098930396	39.0	37.0	39.0	35.0	39.0
4	38.23733658865196	39.0	38.0	40.0	35.0	41.0
5	38.97504065303238	40.0	38.0	41.0	35.0	41.0
6	38.99620330009683	40.0	38.0	41.0	35.0	41.0
7	38.98007721274768	40.0	38.0	41.0	35.0	41.0
8	38.914704763737824	40.0	38.0	41.0	35.0	41.0
9	38.794936678823575	40.0	38.0	41.0	35.0	41.0
10	38.774143155360825	40.0	38.0	41.0	35.0	41.0
11	38.66838133368312	40.0	38.0	41.0	35.0	41.0
12	38.636782375929975	40.0	38.0	41.0	35.0	41.0
13	38.537866478765174	40.0	38.0	41.0	35.0	41.0
14	38.53169486397466	40.0	38.0	41.0	35.0	41.0
15	38.440545722879136	40.0	38.0	41.0	34.0	41.0
16	38.508178394800105	40.0	38.0	41.0	35.0	41.0
17	38.66914133281305	40.0	38.0	41.0	35.0	41.0
18	38.73385067763843	40.0	38.0	41.0	35.0	41.0
19	38.7179928640498	40.0	38.0	41.0	35.0	41.0
20	38.679822187887865	40.0	38.0	41.0	35.0	41.0
21	38.659772843972114	40.0	38.0	41.0	35.0	41.0
22	38.73694076950406	40.0	38.0	41.0	35.0	41.0
23	38.70806739405988	40.0	38.0	41.0	35.0	41.0
24	38.637225324252015	40.0	38.0	41.0	35.0	41.0
25	38.62439989508099	40.0	38.0	41.0	35.0	41.0
26	38.53084200895241	40.0	38.0	41.0	35.0	41.0
27	38.515743668078116	40.0	37.0	41.0	34.0	41.0
28	38.46649714694257	40.0	37.0	41.0	34.0	41.0
29	38.4392423568816	40.0	37.0	41.0	34.0	41.0
30	38.48350357630658	40.0	37.0	41.0	34.0	41.0
31	38.437842253834496	40.0	37.0	41.0	34.0	41.0
32	38.33773119875446	40.0	37.0	41.0	34.0	41.0
33	38.11501585204183	40.0	37.0	41.0	34.0	41.0
34	38.1695624787037	40.0	37.0	41.0	34.0	41.0
35	38.25851510581964	40.0	37.0	41.0	34.0	41.0
36	38.28189873510649	40.0	36.0	41.0	34.0	41.0
37	38.176022418646355	40.0	36.0	41.0	34.0	41.0
38	38.110681629476446	40.0	36.0	41.0	34.0	41.0
39	38.09447987375399	40.0	36.0	41.0	34.0	41.0
40	38.027088879617985	40.0	36.0	41.0	34.0	41.0
41	37.98693269784572	39.0	36.0	41.0	34.0	41.0
42	37.94145591611648	39.0	36.0	41.0	34.0	41.0
43	37.869936765951465	39.0	36.0	41.0	34.0	41.0
44	37.766101875442345	39.0	35.0	41.0	34.0	41.0
45	37.615623741862386	39.0	35.0	41.0	34.0	41.0
46	37.37577632575515	39.0	35.0	40.0	33.0	41.0
47	37.10562571987789	38.0	35.0	40.0	33.0	41.0
48	36.89754220099602	38.0	35.0	40.0	32.0	41.0
49	37.198741750064244	38.0	35.0	40.0	33.0	41.0
50	36.65693452102726	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	14.0
22	33.0
23	75.0
24	173.0
25	343.0
26	581.0
27	1169.0
28	2363.0
29	4484.0
30	8481.0
31	15137.0
32	25789.0
33	41941.0
34	67298.0
35	75262.0
36	119801.0
37	173618.0
38	225980.0
39	355154.0
40	399409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.465651400988854	17.368715588287444	31.1656330107237
2	21.873869147001496	27.113776417879553	33.146640283117804	17.865714152001143
3	23.10146878236011	25.39761532970318	26.287203209793375	25.213712678143335
4	21.03734278465527	26.35140435051714	29.27229259373268	23.33896027109492
5	13.61532179338702	28.779973989969065	30.938621995679938	26.666082220963975
6	18.893459723012285	34.216966898181866	25.151225325570316	21.738348053235534
7	20.34391773289557	32.22627013124322	21.6474513663176	25.78236076954361
8	18.052517060431466	30.21415101242035	28.05668947542922	23.676642451718962
9	19.21169699961835	33.95686658884311	27.080753038513432	19.750683373025108
10	23.76747322370802	27.870545716287644	26.201381972398785	22.160599087605554
11	21.95323072136639	29.47412410594638	23.786390808295	24.786254364392228
12	20.35782578288809	29.237950915789064	27.360166422012423	23.044056879310425
13	20.763993574612734	29.390346231346896	24.265460511354835	25.580199682685535
14	15.114952340210678	32.32211043782674	25.17653665825812	27.38640056370447
15	18.654122616268992	35.33264298431158	27.238223803594604	18.775010595824817
16	17.11764562420449	36.283795407970565	24.76193175563754	21.836627212187405
17	17.3742524423129	43.13749788248291	20.39783614471491	19.09041353048928
18	18.444842717092467	35.61732956212054	24.0445795846964	21.893248136090598
19	21.94795752705643	31.27267885521588	22.29704299037576	24.48232062735193
20	20.718710018475957	28.058205518793333	27.586979692269566	23.63610477046115
21	15.16775019823915	36.039514681561684	26.284237037994025	22.508498082205143
22	22.035228892886263	29.91384259646815	25.63958903360145	22.411339477044137
23	24.41963552999228	29.76817060365551	27.144690519521696	18.667503346830514
24	19.351898053334406	29.695400522178065	26.500240259915746	24.45246116457178
25	20.39747346555954	30.453138826920735	25.02371275507771	24.125674952442015
26	21.553380861120395	32.8074245630671	28.068252494809276	17.570942081003224
27	21.4393675076192	31.032226654710776	23.44188949464496	24.086516343025057
28	19.485437940942386	28.454897411339385	27.78358581430171	24.27607883341652
29	20.511364101277284	32.80444963274301	23.80931275348642	22.87487351249328
30	21.536900121446486	33.248401069279545	25.928928002532142	19.285770806741827
31	20.381224535202257	31.99053022402069	23.679098354956686	23.949146885820365
32	17.082588366436823	35.70055615561055	26.42569180375117	20.791163674201457
33	19.472639505919396	27.61038240069162	31.210595785221816	21.706382308167164
34	19.131975649750473	30.583262436492536	26.838456190111543	23.446305723645448
35	18.347477687573424	28.607248689309323	27.14452081616431	25.900752806952944
36	17.935867035685707	29.530822144078932	28.99564665203315	23.53766416820221
37	18.47255983389875	35.813994370225274	20.76561095464641	24.947834841229568
38	19.88387642524926	30.351128491902497	25.743352181795608	24.021642901052633
39	19.51450850763981	30.5744776033572	28.38571054083988	21.525303348163106
40	18.07591069118587	27.492263964631835	29.083966372632293	25.347858971549996
41	16.357792410230708	36.87966925254971	24.175528040236813	22.58701029698277
42	19.542061376960135	29.828136309195152	27.593885544204642	23.03591676964007
43	18.18261736367834	29.045083308106985	25.168787208864995	27.60351211934968
44	18.7510274836934	29.372565394272026	24.295310197809425	27.58109692422515
45	17.644400382553403	29.007302568868365	30.339635492171503	23.008661556406736
46	18.430703004317557	30.719949336106154	26.624903186604875	24.224444472971413
47	21.431721343825764	28.587609642940564	27.719225471796893	22.261443541436776
48	21.393015775769644	27.11852016875757	27.069322417002002	24.419141638470784
49	16.436579615868162	33.52780790993579	23.398171605562098	26.63744086863395
50	18.1372614484702	31.44112501502424	29.96728054594746	20.4543329905581
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34787.0
1	32536.5
2	21028.0
3	11577.0
4	10811.0
5	8113.0
6	4913.5
7	5638.5
8	9580.5
9	12438.5
10	12395.0
11	10230.0
12	8036.0
13	6867.0
14	6918.5
15	7464.5
16	7453.5
17	6712.0
18	5591.5
19	6369.5
20	8714.5
21	9972.5
22	10464.5
23	14219.5
24	18620.5
25	20203.5
26	21049.0
27	20387.0
28	21593.5
29	27252.5
30	35128.0
31	49624.5
32	71063.0
33	83474.5
34	85394.0
35	86491.0
36	89430.5
37	92600.0
38	94895.5
39	98464.0
40	101950.5
41	106546.5
42	111367.0
43	112456.0
44	113992.5
45	118332.0
46	126997.5
47	130458.0
48	127115.0
49	132650.0
50	145371.5
51	141338.5
52	119535.5
53	94742.5
54	76332.0
55	74527.0
56	72016.5
57	62584.0
58	52305.5
59	39265.5
60	27445.5
61	17922.5
62	10609.0
63	7533.5
64	7592.5
65	7403.5
66	4903.5
67	2505.5
68	1509.5
69	969.5
70	780.5
71	801.0
72	704.0
73	641.5
74	396.5
75	164.0
76	157.0
77	143.0
78	119.0
79	88.0
80	51.5
81	22.0
82	6.5
83	6.0
84	5.5
85	4.0
86	2.5
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	7371.0
25	8018.0
26	8451.0
27	10647.0
28	16072.0
29	13233.0
30	15055.0
31	22649.0
32	32193.0
33	7005.0
34	7669.0
35	6574.0
36	8297.0
37	8582.0
38	6318.0
39	6249.0
40	7967.0
41	8074.0
42	8527.0
43	12515.0
44	38823.0
45	88333.0
46	175261.0
47	131396.0
48	281975.0
49	205458.0
50	374395.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.955330266330053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.60144807608263	5.457471761937297
2	12.270806230700979	1.952366323987338
3	5.0593240618928155	1.2074578151024347
4	2.71068665108485	0.8625763023164864
5	1.653040771235837	0.6575242639445015
6	1.1180274976165843	0.5336566794227078
7	0.7723925244765245	0.430124633921862
8	0.5962633909038524	0.3794777760289604
9	0.4838849686567049	0.3464518262919168
>10	3.849270001174563	6.374090459005615
>50	0.6918622257540987	3.919976819357771
>100	1.607636226388615	32.235249580878005
>500	0.459564242076662	25.48148384481175
>1k	0.11740692315827131	13.839505613990074
>5k	0.005870346157913565	3.3901258075746745
>10k+	0.0025158626391058136	2.93246049142861
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	17285	1.1393395455956632	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	14625	0.964005834789504	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	12045	0.7939453182933043	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	9202	0.6065491755031122	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	9168	0.6043080679213793	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	7916	0.5217825769705103	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	6656	0.4387297665886454	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	6440	0.4244921419517543	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	5923	0.3904141237236398	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	5510	0.36319125809847297	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	4850	0.3196874050413056	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	4724	0.3113821240031191	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	4466	0.2943760723534991	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	3735	0.24619225934624256	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	3458	0.22793382404800716	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	3377	0.22259471480917298	No Hit
CCTCTTTTTCCGGCTGGAACCATGGAGGG	3179	0.20954355889202278	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	3145	0.20730245131028993	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	3134	0.20657738709267043	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	3082	0.20314981079119668	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	2769	0.18251843805347942	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	2733	0.18014550061399756	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT	2660	0.17533371080615937	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2578	0.16992868663845068	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	2392	0.15766850986779443	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	2264	0.14923139897185894	No Hit
TCAAACTTGGAGAAGATAGATTTGTGTCAATCAGTTGAGAGGATTATTGT	2202	0.1451446733816402	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	2025	0.1334777309708544	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1993	0.13136845324687052	No Hit
ACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATA	1897	0.12504062007491892	No Hit
AATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCAT	1844	0.12154712884457063	No Hit
CATTGGACAGACAGAGAGTGAAAGAGAGGGCGAGGGAGAGGGAGAGAA	1838	0.12115163927132365	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	1815	0.11963559590721023	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	1782	0.11746040325435188	No Hit
ACAATCACCAGTCTCAAATGCGCTATTGTTTCTTTTCAGAGTGTTGCAGA	1779	0.11726265846772838	No Hit
TGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATA	1764	0.11627393453461095	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1763	0.11620801960573644	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	1759	0.11594435989023845	No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC	1707	0.11251678358876467	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA	1699	0.1119894641577687	No Hit
CCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACAC	1680	0.11073708050915328	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	1662	0.10955061178941235	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCC	1598	0.10533205634144462	No Hit
ATGATGAAGAGGAGGAAGGATTAGAAGATATTGACGAAGAAGGGGATGA	1584	0.10440924733720167	No Hit
CTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACC	1583	0.10434333240832716	No Hit
AATTACGAAGGCAGTCCAATTAAAGTAACACTGGCAACTTTGAAAATGT	1551	0.1022340546843433	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	1526	0.10058618146248088	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.07178135754432614	0.0	0.0	0.0	0.0
2	0.12167895870231961	0.0	0.0	0.0	0.0
3	0.16610562076372992	0.0	0.0	0.0	0.0
4	0.2223969700225495	0.0	0.0	0.0	0.0
5	0.26642814251071284	0.0	0.0	0.0	0.0
6	0.38421812040943715	0.0	0.0	0.0	0.0
7	0.45988845875735856	0.0	0.0	0.0	0.0
8	0.5401069271976202	0.0	0.0	0.0	0.0
9	0.6108995608088289	0.0	0.0	0.0	0.0
10	0.6662681010634055	0.0	0.0	0.0	0.0
11	0.733830903159764	0.0	0.0	0.0	0.0
12	0.7899245076319601	0.0	0.0	0.0	0.0
13	0.8427223656604313	0.0	0.0	3.95489573246976E-4	0.0
14	0.8943996698980362	0.0	0.0	3.95489573246976E-4	0.0
15	0.9584689807640463	0.0	0.0	3.95489573246976E-4	0.0
16	1.012123732867886	0.0	0.0	3.95489573246976E-4	0.0
17	1.0944514790321316	0.0	0.0	3.95489573246976E-4	0.0
18	1.1420420576795176	0.0	0.0	3.95489573246976E-4	0.0
19	1.1937193619171225	0.0	0.0	3.95489573246976E-4	0.0
20	1.2496811365315696	0.0	0.0	3.95489573246976E-4	0.0
21	1.2977990346099517	0.0	0.0	3.95489573246976E-4	0.0
22	1.3337885857754266	0.0	0.0	3.95489573246976E-4	0.0
23	1.372942053526877	0.0	0.0	3.95489573246976E-4	0.0
24	1.4131501601403198	0.0	0.0	3.95489573246976E-4	0.0
25	1.4541492459002563	0.0	0.0	3.95489573246976E-4	0.0
26	1.4956097361623142	0.0	0.0	3.95489573246976E-4	0.0
27	1.5277762214530684	0.0	0.0	3.95489573246976E-4	0.0
28	1.566666029489021	0.0	0.0	3.95489573246976E-4	0.0
29	1.6106972019771841	0.0	0.0	3.95489573246976E-4	0.0
30	1.6475436472180274	0.0	0.0	3.95489573246976E-4	0.0
31	1.680303366868652	0.0	0.0	3.95489573246976E-4	0.0
32	1.7164906628207504	0.0	0.0	3.95489573246976E-4	0.0
33	1.7529416184883466	0.0	0.0	3.95489573246976E-4	0.0
34	1.7876128710763315	0.0	0.0	3.95489573246976E-4	0.0
35	1.82571169996579	0.0	0.0	3.95489573246976E-4	0.0
36	1.8544506089550703	0.0	0.0	3.95489573246976E-4	0.0
37	1.8737636831152977	0.0	0.0	3.95489573246976E-4	0.0
38	1.8913629691247882	0.0	0.0	3.95489573246976E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGGT	95	0.0	162.40886	44
CTCGTTA	45	0.0	162.40886	44
GCGACCC	85	0.0	162.40886	44
AACGGTA	45	0.0	162.40886	44
GGGGATA	110	0.0	162.40884	44
ATTACGC	10	0.0050506354	162.40884	44
TTGCGGA	10	0.0050506354	162.40884	44
AGCTCGC	125	0.0	162.40884	44
ATTGCAC	15	7.825137E-5	162.40884	44
ACAAGGT	10	0.0050506354	162.40884	44
TTATTGT	265	0.0	159.34453	44
GTGTTAT	135	0.0	156.3937	44
GCACAGT	60	0.0	148.87477	44
ACTGGTA	55	0.0	147.64441	44
ATGACGT	150	0.0	146.16797	44
TTCTAAC	45	0.0	144.36343	44
GGTTTCC	90	0.0	144.36343	44
CACTTAC	75	0.0	140.75433	44
TACAAGG	135	0.0	138.34828	44
GTCATAA	90	0.0	135.34071	44
>>END_MODULE