FastQCFastQC Report
Tue 24 May 2016
ERR841062.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841062.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3500564
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC517881.4794187450936478No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA293740.8391219243527615No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT287780.8220960965147331No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA179690.5133172825864631No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA164530.4700099755353708No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT159210.4548124245121643No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC156780.4478706859808877No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT112350.3209482814769277No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC100340.2866395243737866No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA93880.2681853552741787No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA86480.24704590460280115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA77530.221478596020527No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA76890.21965031920570516No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA71600.20453846865819336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA69500.19853943535955923No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG68770.19645405711765304No Hit
ATTAATTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATTTC68180.19476861442898916No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC59600.17025827838028387No Hit
AAAAAAAAAAAAAAAAAAAAAAAA56430.16120259478186943No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA51680.1476333527968636No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT51460.14700488264176859No Hit
TTTGGATGAGAATGGCTGTTACTACGAGGGCTATGTGGCTGATTGAAGAG49840.1423770569542508No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA47930.1369207933350169No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC47920.13689222650978528No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA46780.1336356084333839No Hit
CACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACA40160.11472437013007046No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG39820.11375309807219636No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA39660.1132960288684909No Hit
ATTAATTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATT37860.10815400032680449No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT37520.10718272826893038No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT36470.10418321161961329No Hit
AGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGA35730.10206926655247554No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACGAGCTACCT35610.10172646464969644No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC35260.10072662576659076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCGG650.0188.3788344
CGCGATA400.0188.3788144
TGATACA1050.0179.408444
CGTGCAT950.0178.4641444
TCGTAGA800.0176.6051344
CGGGTAG1350.0174.4248444
GAGTCAT550.0171.2534644
GCCGCCG1100.0171.2534644
AGTTTCG800.0164.8314744
CTATAAG1100.0162.690844
TGGACTC700.0161.4675644
TTACCGC600.0156.9823544
CGTTTGG1450.0155.899744
TAGTGTA1150.0147.426944
ATTATGC1250.0143.1679144
TTTGGCG1150.0139.2365144
TCCGCCC304.3553664E-6125.58588444
TTTCGTC451.7680577E-9125.5858844
TGTATCC1750.0123.791844
TCACTCG1650.0114.1689844
Produced by FastQC (version 0.11.5)