##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841062.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3500564
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.77462460334963	35.0	35.0	39.0	27.0	39.0
2	35.559566686968154	35.0	35.0	39.0	32.0	39.0
3	35.87963111087242	35.0	35.0	39.0	33.0	39.0
4	36.13087319643349	35.0	35.0	39.0	33.0	39.0
5	36.865169726935434	35.0	35.0	41.0	34.0	41.0
6	36.903040195808444	35.0	35.0	41.0	34.0	41.0
7	36.90561549510308	35.0	35.0	41.0	34.0	41.0
8	36.89318978313209	35.0	35.0	41.0	34.0	41.0
9	36.858237986792986	35.0	35.0	40.0	34.0	41.0
10	36.81132811741194	35.0	35.0	40.0	34.0	41.0
11	36.760476597485436	35.0	35.0	40.0	34.0	41.0
12	36.75196511190768	35.0	35.0	40.0	34.0	41.0
13	36.71248233141859	35.0	35.0	40.0	34.0	41.0
14	36.63968891869996	35.0	35.0	40.0	34.0	41.0
15	36.63370045512666	35.0	35.0	40.0	33.0	41.0
16	36.674748697638435	35.0	35.0	40.0	34.0	41.0
17	36.77110631315411	35.0	35.0	40.0	34.0	41.0
18	36.761066502426466	35.0	35.0	40.0	34.0	41.0
19	36.71343274969405	35.0	35.0	41.0	34.0	41.0
20	36.68464681691293	35.0	35.0	41.0	34.0	41.0
21	36.735282657308936	35.0	35.0	41.0	34.0	41.0
22	36.74500480494	35.0	35.0	41.0	34.0	41.0
23	36.766400214365454	35.0	35.0	41.0	34.0	41.0
24	36.67868634882836	35.0	35.0	40.0	34.0	41.0
25	36.695340892362616	35.0	35.0	40.0	34.0	41.0
26	36.68811162399485	35.0	35.0	41.0	34.0	41.0
27	36.714521954980306	35.0	35.0	41.0	34.0	41.0
28	36.70969403116305	35.0	35.0	40.0	34.0	41.0
29	36.719736046612546	35.0	35.0	40.0	34.0	41.0
30	36.75924223955866	35.0	35.0	41.0	34.0	41.0
31	36.72294571784446	35.0	35.0	40.0	34.0	41.0
32	36.695258656682654	35.0	35.0	40.0	34.0	41.0
33	36.55250409470912	35.0	35.0	40.0	33.0	41.0
34	36.56349689694073	35.0	35.0	40.0	33.0	41.0
35	36.55637793738389	35.0	35.0	40.0	34.0	41.0
36	36.591430238137626	35.0	35.0	40.0	34.0	41.0
37	36.575667640003616	35.0	35.0	40.0	34.0	41.0
38	36.51933519317294	35.0	35.0	40.0	34.0	41.0
39	36.489313355299856	35.0	35.0	40.0	34.0	41.0
40	36.46326774697445	35.0	35.0	40.0	34.0	41.0
41	36.37677153737341	35.0	35.0	40.0	34.0	41.0
42	36.316560262615155	35.0	35.0	39.0	34.0	41.0
43	36.26434517079029	35.0	35.0	39.0	34.0	41.0
44	36.15627018064122	35.0	35.0	39.0	33.0	41.0
45	36.040036126220976	35.0	35.0	39.0	33.0	41.0
46	35.87668984544864	35.0	35.0	39.0	33.0	41.0
47	35.63762993369517	35.0	35.0	38.0	32.0	41.0
48	35.623500904312785	35.0	35.0	38.0	32.0	40.0
49	35.65125924095774	35.0	34.0	38.0	32.0	40.0
50	35.82931844730226	36.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	11.0
21	25.0
22	120.0
23	295.0
24	814.0
25	1929.0
26	4048.0
27	7816.0
28	14339.0
29	25264.0
30	42977.0
31	72479.0
32	122782.0
33	239885.0
34	998053.0
35	419478.0
36	101724.0
37	153514.0
38	229687.0
39	445895.0
40	619429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.04788828314523	18.0822004682674	30.86991124858737
2	23.43885156791877	25.79907123537807	28.654468251401777	22.107608945301386
3	18.87841502112231	27.684481700663095	26.920547660319883	26.516555617894717
4	18.476136988211042	28.288698621136483	26.491445378516147	26.74371901213633
5	14.06770451847188	32.61248758771444	26.90566434437422	26.414143549439462
6	17.23967909171208	30.853828125981984	26.647791613008646	25.258701169297293
7	18.641167537573942	31.014402250608757	23.950454840991338	26.393975370825956
8	17.951821477910418	29.916064954104538	26.857557810684224	25.274555757300828
9	19.580930387217602	32.10534073937799	27.398556346920095	20.915172526484305
10	23.844986122236303	25.036337001694587	28.149006845754	22.969670030315115
11	19.20836185254719	28.645526835104285	26.128503863948783	26.017607448399744
12	18.997852917415596	25.650780845600877	27.968721611717427	27.3826446252661
13	18.902011218763604	28.84843699472428	26.899608177425122	25.349943609086996
14	17.504979197637866	27.94312573630992	24.211669890909008	30.340225175143203
15	19.36150860261375	33.671659766826146	25.63529762632536	21.331534004234744
16	17.07959060311424	37.531380657516905	21.71938579040406	23.669642948964796
17	18.695501639164434	37.78268301907921	21.44791525022825	22.07390009152811
18	20.45573227628462	29.64879373723777	23.159182348901492	26.736291637576116
19	21.83782384781424	25.98504126763573	23.438423065540295	28.738711819009737
20	19.963154508816295	25.19705396044752	27.482142877547734	27.35764865318846
21	18.939519460292683	35.46288540932262	22.819951299276344	22.777643831108357
22	19.357823482158874	28.8622919049616	23.613851939287496	28.166032673592028
23	24.248263994030676	28.432389752051385	26.28787818191583	21.031468072002113
24	19.252783265782313	31.946851992993132	25.954446197812697	22.845918543411862
25	22.21340821058249	30.411397427522562	22.502995933351123	24.872198428543825
26	19.281403444543532	35.76670390440107	24.17083286146549	20.781059789589907
27	21.67168272766616	32.133902489636824	22.777398941723177	23.41701584097384
28	21.699996576646114	29.855303316469172	21.651455174106804	26.79324493277791
29	20.330088832729984	31.575056228367337	24.84806940691392	23.246785531988756
30	24.835748389420477	29.71216860622255	27.156266282481422	18.295816721875553
31	22.065652082617493	31.887694231283163	22.14540860153476	23.90124508456458
32	20.65129893889499	29.35682369927716	26.073911452616173	23.91796590921168
33	19.876574430342338	26.87059729888316	29.59978507800815	23.653043192766354
34	21.258421238358487	30.417205962884843	26.284558628811844	22.039814169944822
35	19.07328631363762	28.3092121133745	24.865581035610457	27.751920537377423
36	16.560634069216707	29.858322158469292	27.49453052631973	26.086513245994276
37	16.241868131146127	32.08595400630932	25.98011839606836	25.69205946647619
38	22.496187789370026	28.14318964586008	24.76184674285226	24.598775821917638
39	18.72125392986242	29.547308807550372	27.874008957001607	23.8574283055856
40	17.434684506997808	27.574325070609213	28.643956009277815	26.347034413115168
41	18.66067912045884	29.519660249810357	25.384321189809523	26.43533943992128
42	18.285883164220273	29.350453316215468	27.682859784988604	24.680803734575647
43	17.42843544976587	25.745446763385015	27.59079323099331	29.2353245558558
44	18.439467990339406	25.81796985046457	25.117541404267957	30.625020754928066
45	16.169443415444228	26.92627178330652	32.67035421919279	24.233930582056466
46	17.96449138535012	29.058842485059916	28.23570304848354	24.740963081106425
47	18.265391741022142	28.580429036172195	28.16354769720586	24.990631525599802
48	21.08530683919417	27.412107675936376	25.837956050803363	25.66462943406609
49	16.280308927431225	30.286825039851283	26.207854758265327	27.225011274452164
50	17.831611642541464	31.704904530944678	26.69822950254889	23.765254323964964
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	61763.0
1	59909.5
2	35602.5
3	12936.5
4	12165.0
5	9271.0
6	5022.0
7	3157.0
8	3306.5
9	3509.5
10	3583.0
11	3383.5
12	3095.5
13	3045.0
14	3576.5
15	4270.5
16	4886.5
17	7221.0
18	10491.0
19	10936.5
20	10190.5
21	14276.0
22	20726.0
23	25523.0
24	29483.5
25	33905.5
26	34781.5
27	36186.0
28	45701.5
29	56496.5
30	64925.5
31	83385.0
32	119075.0
33	144635.5
34	169550.0
35	206131.0
36	212699.5
37	195742.0
38	190293.0
39	201165.5
40	228008.0
41	273957.0
42	304925.5
43	302578.5
44	296449.0
45	299477.5
46	312396.0
47	309408.0
48	324527.0
49	366360.0
50	383844.0
51	377577.5
52	329820.0
53	258124.0
54	214425.5
55	184438.5
56	149345.0
57	123461.0
58	102756.5
59	83517.5
60	66777.0
61	49653.5
62	37114.5
63	27938.5
64	19032.5
65	13463.5
66	8548.5
67	5108.0
68	4277.0
69	3439.5
70	1939.0
71	1033.0
72	803.0
73	716.0
74	543.5
75	321.0
76	131.0
77	52.5
78	16.5
79	5.0
80	2.5
81	3.0
82	3.0
83	2.5
84	2.0
85	1.0
86	0.0
87	0.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	22516.0
25	25457.0
26	16628.0
27	18262.0
28	18616.0
29	17383.0
30	28998.0
31	59439.0
32	58603.0
33	9780.0
34	8074.0
35	9461.0
36	11126.0
37	11717.0
38	7548.0
39	8106.0
40	10294.0
41	11746.0
42	16070.0
43	29067.0
44	94439.0
45	245369.0
46	384210.0
47	400463.0
48	633446.0
49	600678.0
50	743068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.841386561236848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.39352279387312	5.021134605238338
2	10.405645478717195	1.4237808627818158
3	4.006284214752393	0.82225616961907
4	2.1199346946743174	0.5801317092337845
5	1.3064968595870798	0.4469125028738596
6	0.8757501211217646	0.359480706578639
7	0.6249019835754227	0.29926372227662024
8	0.4784542455063383	0.26186323562990227
9	0.39138153715170065	0.24098331497272785
>10	3.3338106977698447	4.737261807371825
>50	0.6471592025471316	3.179931899588906
>100	1.649472209558061	30.006179834525902
>500	0.6126739024369882	29.267513005040918
>1k	0.1451069768929709	14.760958068084046
>5k	0.00537433247751744	2.549240095119921
>10k+	0.00403074935813808	6.043108461063725
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	51788	1.4794187450936478	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	29374	0.8391219243527615	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	28778	0.8220960965147331	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	17969	0.5133172825864631	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	16453	0.4700099755353708	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	15921	0.4548124245121643	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	15678	0.4478706859808877	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	11235	0.3209482814769277	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	10034	0.2866395243737866	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	9388	0.2681853552741787	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	8648	0.24704590460280115	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	7753	0.221478596020527	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	7689	0.21965031920570516	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	7160	0.20453846865819336	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6950	0.19853943535955923	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	6877	0.19645405711765304	No Hit
ATTAATTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATTTC	6818	0.19476861442898916	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	5960	0.17025827838028387	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	5643	0.16120259478186943	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5168	0.1476333527968636	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	5146	0.14700488264176859	No Hit
TTTGGATGAGAATGGCTGTTACTACGAGGGCTATGTGGCTGATTGAAGAG	4984	0.1423770569542508	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	4793	0.1369207933350169	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	4792	0.13689222650978528	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA	4678	0.1336356084333839	No Hit
CACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACA	4016	0.11472437013007046	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG	3982	0.11375309807219636	No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA	3966	0.1132960288684909	No Hit
ATTAATTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATT	3786	0.10815400032680449	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	3752	0.10718272826893038	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	3647	0.10418321161961329	No Hit
AGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGA	3573	0.10206926655247554	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACGAGCTACCT	3561	0.10172646464969644	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	3526	0.10072662576659076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03770820930570045	0.0	0.0	0.0	0.0
2	0.06304698328612189	0.0	0.0	0.0	0.0
3	0.0943847905651775	0.0	0.0	0.0	0.0
4	0.1384348350722912	0.0	0.0	0.0	0.0
5	0.1730863940782114	0.0	0.0	0.0	0.0
6	0.24418922207964203	0.0	0.0	0.0	0.0
7	0.2814689290068686	0.0	0.0	0.0	0.0
8	0.330432467453816	0.0	0.0	0.0	0.0
9	0.3794531395512266	0.0	0.0	0.0	0.0
10	0.4225604788256978	0.0	0.0	0.0	0.0
11	0.46815313189531743	0.0	0.0	0.0	0.0
12	0.5131744484603052	0.0	0.0	0.0	0.0
13	0.5618237518297051	0.0	0.0	0.0	0.0
14	0.6067879347442298	0.0	0.0	0.0	0.0
15	0.6552658371622402	0.0	0.0	0.0	0.0
16	0.7043722097353455	0.0	0.0	0.0	0.0
17	0.7508504343871445	0.0	0.0	0.0	0.0
18	0.7949290457194897	0.0	0.0	0.0	0.0
19	0.8415501044974467	0.0	0.0	0.0	0.0
20	0.8838004390149702	0.0	0.0	0.0	0.0
21	0.9219371506991445	0.0	0.0	0.0	0.0
22	0.9561887741518224	0.0	0.0	0.0	0.0
23	0.9932685133024278	0.0	0.0	0.0	0.0
24	1.0303196856278016	0.0	0.0	0.0	0.0
25	1.0670280560503964	0.0	0.0	0.0	0.0
26	1.1063931412195291	0.0	0.0	0.0	0.0
27	1.1439870832243033	0.0	0.0	0.0	0.0
28	1.182438029986025	0.0	0.0	0.0	0.0
29	1.223888493397064	0.0	0.0	0.0	0.0
30	1.2665101966425982	0.0	0.0	0.0	0.0
31	1.3035899357932037	0.0	0.0	0.0	0.0
32	1.339412734633619	0.0	0.0	0.0	0.0
33	1.3794062899578468	0.0	0.0	0.0	0.0
34	1.4231706662126447	0.0	0.0	0.0	0.0
35	1.4561082157046692	0.0	0.0	0.0	0.0
36	1.4864461841006191	0.0	0.0	0.0	0.0
37	1.5071285655682913	0.0	0.0	0.0	0.0
38	1.525525601017436	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACTCGG	65	0.0	188.37883	44
CGCGATA	40	0.0	188.37881	44
TGATACA	105	0.0	179.4084	44
CGTGCAT	95	0.0	178.46414	44
TCGTAGA	80	0.0	176.60513	44
CGGGTAG	135	0.0	174.42484	44
GAGTCAT	55	0.0	171.25346	44
GCCGCCG	110	0.0	171.25346	44
AGTTTCG	80	0.0	164.83147	44
CTATAAG	110	0.0	162.6908	44
TGGACTC	70	0.0	161.46756	44
TTACCGC	60	0.0	156.98235	44
CGTTTGG	145	0.0	155.8997	44
TAGTGTA	115	0.0	147.4269	44
ATTATGC	125	0.0	143.16791	44
TTTGGCG	115	0.0	139.23651	44
TCCGCCC	30	4.3553664E-6	125.585884	44
TTTCGTC	45	1.7680577E-9	125.58588	44
TGTATCC	175	0.0	123.7918	44
TCACTCG	165	0.0	114.16898	44
>>END_MODULE