##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841061.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2950255
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.26988921296633	39.0	37.0	39.0	34.0	39.0
2	37.82421722868023	39.0	38.0	39.0	35.0	39.0
3	38.12297614951928	39.0	38.0	39.0	35.0	39.0
4	38.90363341473873	39.0	39.0	41.0	35.0	41.0
5	39.311170729309836	40.0	39.0	41.0	35.0	41.0
6	39.29068097503436	40.0	39.0	41.0	35.0	41.0
7	39.32247992122715	40.0	39.0	41.0	35.0	41.0
8	39.291251773151814	40.0	39.0	41.0	35.0	41.0
9	39.26306200650452	40.0	39.0	41.0	35.0	41.0
10	39.20830233318815	40.0	39.0	41.0	35.0	41.0
11	39.125210871602626	40.0	38.0	41.0	35.0	41.0
12	39.0987250254639	40.0	38.0	41.0	35.0	41.0
13	38.991320072332734	40.0	38.0	41.0	35.0	41.0
14	38.91363255040666	40.0	38.0	41.0	35.0	41.0
15	38.888690638605816	40.0	38.0	41.0	35.0	41.0
16	38.853446566483235	40.0	38.0	41.0	35.0	41.0
17	38.84381655145064	40.0	38.0	41.0	35.0	41.0
18	38.76688896383533	40.0	38.0	41.0	35.0	41.0
19	38.73497409545954	40.0	38.0	41.0	35.0	41.0
20	38.59474452208369	40.0	38.0	41.0	35.0	41.0
21	38.52125731504565	40.0	38.0	41.0	35.0	41.0
22	38.488997391750885	40.0	38.0	41.0	35.0	41.0
23	38.327308656370384	40.0	37.0	41.0	34.0	41.0
24	38.15274171215709	40.0	37.0	41.0	34.0	41.0
25	38.05387823469933	39.0	37.0	41.0	34.0	41.0
26	37.915368312296124	39.0	36.0	41.0	33.0	41.0
27	37.836024923331216	39.0	36.0	41.0	33.0	41.0
28	37.63395761740132	39.0	36.0	41.0	33.0	41.0
29	37.63071235928012	39.0	36.0	41.0	33.0	41.0
30	37.48928732300582	39.0	35.0	41.0	33.0	41.0
31	37.433936802171	39.0	35.0	41.0	33.0	41.0
32	37.28058675074174	38.0	35.0	40.0	32.0	41.0
33	37.150884657329556	38.0	35.0	40.0	32.0	41.0
34	37.104711413474206	38.0	35.0	40.0	32.0	41.0
35	36.88907912459369	38.0	35.0	40.0	32.0	41.0
36	36.78860600275068	38.0	35.0	40.0	32.0	41.0
37	36.80363530996175	38.0	35.0	40.0	32.0	41.0
38	36.46998104853071	37.0	34.0	40.0	31.0	41.0
39	36.46200023011836	37.0	34.0	40.0	31.0	41.0
40	36.19647494755359	37.0	34.0	40.0	31.0	41.0
41	36.14747296481915	37.0	34.0	40.0	31.0	41.0
42	36.01210601378475	36.0	34.0	40.0	31.0	41.0
43	35.78036849106056	36.0	34.0	40.0	30.0	41.0
44	35.56886354018149	36.0	34.0	40.0	29.0	41.0
45	35.30075537657242	36.0	33.0	40.0	29.0	41.0
46	34.93074781780488	35.0	33.0	39.0	28.0	41.0
47	35.1161312124868	35.0	33.0	39.0	29.0	40.0
48	36.135850532787444	37.0	34.0	39.0	31.0	41.0
49	36.50092680445398	37.0	35.0	39.0	32.0	41.0
50	37.150062298755934	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	17.0
22	58.0
23	108.0
24	266.0
25	517.0
26	945.0
27	2039.0
28	4242.0
29	8896.0
30	17999.0
31	35307.0
32	65007.0
33	109356.0
34	172277.0
35	190353.0
36	321643.0
37	501458.0
38	494308.0
39	453125.0
40	572326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	38.61198438779021	19.11739832658533	42.27061728562446
2	20.045690965696185	16.31048841540816	40.972966743552675	22.670853875342978
3	20.64065648562582	26.643561319275793	27.5103338524975	25.205448342600896
4	19.152005504608923	28.227017664574756	32.08458251913818	20.536394311678144
5	15.357180989439895	27.188361683990024	32.49895348029238	24.955503846277693
6	18.658149888738432	21.640502261668907	27.546500217777787	32.154847631814874
7	21.45201008048457	19.046523097156008	23.503900510294873	35.99756631206455
8	22.3303409366309	21.387236018581447	33.68413916763127	22.59828387715638
9	22.55544012297242	28.69145209481892	25.618260116498405	23.13484766571025
10	31.70153088461845	18.630559053369964	25.78285605820514	23.88505400380645
11	23.63958369700246	19.09550191424131	24.59333176284762	32.671582625908606
12	21.564847784344067	22.651567406885167	23.23775402465211	32.545830784118664
13	25.41485397025003	20.382983843769438	22.643771470601692	31.55839071537884
14	19.307686962652383	21.49553174217144	21.860178187987138	37.336603107189035
15	23.090071875142996	34.20789050438013	21.400929750140243	21.301107870336633
16	20.453723491698174	34.01973727694725	20.949036608699924	24.577502622654652
17	20.312040823589825	35.77107741534206	20.404609093112292	23.512272667955823
18	20.336140435318303	23.16952263448414	21.292600131175103	35.20173679902246
19	24.254988128144856	20.933851480634726	20.909209542903916	33.9019508483165
20	21.520207575277393	19.839335921810147	27.261372322053518	31.37908418085894
21	22.596385736148232	33.149066775583805	21.852077193327357	22.402470294940606
22	25.773263667038947	22.4401280567273	19.784594890950107	32.00201338528365
23	27.329434235345758	20.845181179253995	30.445571653975673	21.37981293142457
24	23.936337706401652	24.143709611542054	21.96196599954919	29.957986682507105
25	24.85209155377916	22.4808922510205	20.918976335329504	31.748039859870836
26	23.683687450714082	34.44070208809799	23.001715705979656	18.87389475520827
27	25.349863136285585	25.0631354601692	20.671596901538948	28.915404502006268
28	24.613314562727318	21.10821519554348	21.002786189768752	33.275684051960454
29	21.62520982331364	26.16163682010464	29.680685959629056	22.532467396952665
30	25.591484414999428	26.381086207813066	29.27982497125287	18.74760440593464
31	26.42679184933613	31.554166200538525	21.13917495671165	20.8798669934137
32	20.831697510989365	26.41263363444265	24.18728982854222	28.56837902602577
33	22.444797318505497	21.895843405623427	34.671741022583426	20.98761825328765
34	25.09820669768962	31.842867850545243	23.930618275832913	19.12830717593223
35	20.554865755842627	22.200146227925206	24.17375453897825	33.07123347725391
36	20.601123774815605	23.303094225312062	25.46943640067084	30.62634559920149
37	21.03643146252522	38.185771118348036	19.52041900464528	21.257378414481465
38	30.455765980866783	24.172064097885144	19.74620714117159	25.625962780076478
39	21.020574941880362	33.72664888658115	23.189698662953305	22.063077508585184
40	19.222240546274673	25.135944659380627	25.387293705836555	30.254521088508145
41	19.801516659319454	33.3245566297758	22.4867385008017	24.387188210103048
42	19.57683122852503	34.4265828719667	21.334353083826663	24.66223281568161
43	20.653443622594907	21.894255132887043	23.130863352155163	34.32143789236289
44	20.331119984633574	24.256586560309326	21.226714161007063	34.18557929405004
45	20.476234492337976	23.701012109674917	34.81656448972318	21.006188908263926
46	19.9274004146362	26.691344660602677	30.425174231354756	22.956080693406367
47	19.552210470877146	34.20513628485899	26.18649026473095	20.056162979532914
48	29.147374389172935	22.074784035755943	22.85701729803174	25.920824277039383
49	18.006106367059598	25.00038382293787	21.43840460669408	35.55510520330845
50	17.790179971154448	27.760434700816017	32.15133826452297	22.298047063506562
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	26378.0
1	22298.0
2	23827.0
3	28459.0
4	24975.0
5	16355.5
6	7741.5
7	5422.5
8	5503.0
9	5208.0
10	4926.5
11	4838.0
12	4786.0
13	4380.5
14	3998.5
15	4147.5
16	4387.5
17	4442.0
18	4577.5
19	4794.5
20	5008.5
21	5824.0
22	6963.5
23	8486.0
24	10182.0
25	12174.0
26	14339.5
27	16745.5
28	19460.0
29	21496.5
30	26655.0
31	47453.0
32	80020.0
33	88668.5
34	80854.0
35	81891.5
36	79458.0
37	76650.0
38	78672.5
39	81281.0
40	86304.0
41	96919.5
42	110063.5
43	119196.0
44	126948.0
45	141783.0
46	170388.5
47	190257.0
48	235710.5
49	303938.5
50	382186.5
51	441487.0
52	380895.0
53	283750.5
54	248714.0
55	240227.0
56	236441.5
57	235206.5
58	230998.0
59	211301.0
60	174088.5
61	145628.0
62	128550.0
63	107475.5
64	88670.0
65	78703.0
66	60928.5
67	41567.0
68	30975.5
69	23270.0
70	17423.0
71	11942.5
72	8113.5
73	5580.5
74	3748.0
75	2417.5
76	1743.5
77	1201.5
78	745.5
79	520.0
80	408.5
81	309.0
82	217.0
83	144.0
84	98.5
85	57.0
86	18.5
87	9.0
88	6.0
89	5.0
90	2.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	12965.0
25	14295.0
26	15358.0
27	16592.0
28	16007.0
29	15622.0
30	16850.0
31	17789.0
32	17984.0
33	15741.0
34	17300.0
35	16616.0
36	13491.0
37	14569.0
38	17428.0
39	19376.0
40	22325.0
41	20933.0
42	32017.0
43	55569.0
44	159712.0
45	310244.0
46	295271.0
47	408124.0
48	411064.0
49	610232.0
50	366781.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.910168264992029
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.2084020844699	7.05687246538306
2	10.95552424633264	2.171421774247129
3	3.902330970134902	1.160182696191794
4	1.9533200200401644	0.7743092029590252
5	1.1764473621425582	0.5829395656869383
6	0.7887100608543156	0.4689749649215023
7	0.5651968998494502	0.39208374662519296
8	0.48107883338142565	0.38140577500287937
9	0.36473992513217574	0.32531706279184114
>10	4.48382352865992	10.94839288667552
>50	2.239428620869662	16.044202303882678
>100	1.7423734505303066	29.386179811455477
>500	0.07850254007883758	5.262174001939991
>1k	0.0539944300054444	10.929691110555408
>5k	0.0026805745392773812	1.5914678889186973
>10k+	0.003446452979070919	12.524384742762882
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	112031	3.797332772929798	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	72696	2.46405819158005	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	42506	1.4407568159362496	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	35466	1.2021333749116603	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	20264	0.6868558819491875	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	13307	0.45104575706167777	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	11587	0.39274571181135187	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	11411	0.38678012578573717	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	10756	0.3645786550654096	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	7292	0.24716507556126502	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	6699	0.22706511809996083	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	6078	0.20601609013458158	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	5730	0.19422049958393428	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	5520	0.18710247080337125	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	5509	0.1867296216767703	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC	5108	0.17313757624340947	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT	4812	0.16310454520033013	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	4660	0.15795244817820833	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	4409	0.14944470901667822	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	4392	0.14886848763920407	No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC	4392	0.14886848763920407	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	4307	0.14598738075183332	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT	4292	0.14547895012465023	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	4259	0.14436040274484746	No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTT	4182	0.14175045885864102	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT	4017	0.13615772195962722	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	3969	0.13453074395264136	No Hit
CCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGC	3827	0.12971760068197496	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	3720	0.12609079554140237	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	3668	0.12432823603383436	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	3575	0.12117596614529931	No Hit
CTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCATCT	3516	0.11917613901171253	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	3470	0.1176169517550178	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	3452	0.1170068350023981	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3443	0.11670177662608824	No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT	3418	0.1158543922474498	No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT	3278	0.11110903972707445	No Hit
CCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCTT	3135	0.10626200108126246	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAA	3023	0.10246571906496217	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03620026065543487	0.0	0.0	0.0	0.0
2	0.0648418526534147	0.0	0.0	0.0	0.0
3	0.08134890034929185	0.0	0.0	0.0	0.0
4	0.09772036654458682	0.0	0.0	0.0	0.0
5	0.11568491537172211	0.0	0.0	0.0	0.0
6	0.14090307448000258	0.0	0.0	0.0	0.0
7	0.15937605393432094	0.0	0.0	0.0	0.0
8	0.19127160194627243	0.0	0.0	0.0	0.0
9	0.21384592179320092	0.0	0.0	0.0	0.0
10	0.23181047062033622	0.0	0.0	0.0	0.0
11	0.2497750194474715	0.0	0.0	0.0	0.0
12	0.27475591092973317	0.0	0.0	0.0	0.0
13	0.29329668113434265	0.0	0.0	0.0	0.0
14	0.31061721783371266	0.0	0.0	0.0	0.0
15	0.334377875810735	0.0	0.0	0.0	0.0
16	0.3578334754114475	0.0	0.0	0.0	0.0
17	0.3912543153049482	0.0	0.0	0.0	0.0
18	0.4311152764761012	0.0	0.0	0.0	0.0
19	0.454842039077978	0.0	0.0	0.0	0.0
20	0.47741635892490647	0.0	0.0	0.0	0.0
21	0.4998550972712528	0.0	0.0	0.0	0.0
22	0.5179552275989703	0.0	0.0	0.0	0.0
23	0.5416819902008471	0.0	0.0	0.0	0.0
24	0.5617819476621513	0.0	0.0	0.0	0.0
25	0.5800854502407419	0.0	0.0	0.0	0.0
26	0.6102862294954164	0.0	0.0	0.0	0.0
27	0.6313691528359413	0.0	0.0	0.0	0.0
28	0.6556043460650012	0.0	0.0	0.0	0.0
29	0.682042738678521	0.0	0.0	0.0	0.0
30	0.7064813041584541	0.0	0.0	0.0	0.0
31	0.7286488795036361	0.0	0.0	0.0	0.0
32	0.7522739559800763	0.0	0.0	0.0	0.0
33	0.7755939740802066	0.0	0.0	0.0	0.0
34	0.7993885274323745	0.0	0.0	0.0	0.0
35	0.81894615889135	0.0	0.0	0.0	0.0
36	0.8366056493421755	0.0	0.0	0.0	0.0
37	0.8532143831634893	0.0	0.0	0.0	0.0
38	0.8633829957071507	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGCA	590	0.0	256.68213	44
CTATCAT	5265	0.0	224.60397	44
ACGATTC	55	1.6916601E-10	175.75526	44
ACTTCTA	2330	0.0	155.57736	44
ACATTGG	1435	0.0	150.44324	44
TAATTCC	425	0.0	132.67798	44
CATGACC	625	0.0	128.88719	44
ACCACTC	590	0.0	128.34106	44
ACCGCGA	105	0.0	122.74971	44
CATCTCG	40	2.5409443E-4	120.83175	44
TGTTTTT	1635	0.0	114.30363	44
CCCATAC	1460	0.0	108.141655	44
TGACGGC	95	7.6306605E-9	101.753044	44
CCTTAGC	14765	0.0	94.99074	43
ACGTCGA	85	6.1118044E-10	94.77	44
CAGGCAC	1440	0.0	91.74262	44
CGATATC	20	8.0437755E-4	90.44744	43
CCTATCA	5135	0.0	86.42559	43
ACAGGCC	95	9.076066E-7	84.794205	44
CGTATCC	15	0.0010436756	84.710014	42
>>END_MODULE