FastQCFastQC Report
Tue 24 May 2016
ERR841060.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841060.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724418
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC176462.435886463340226No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT150692.0801526190679964No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA113341.5645663139237294No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC84951.1726655052745791No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT66050.9117664111051906No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA54340.7501194061991834No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA31190.4305525263038743No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC20410.2817434133331861No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG18830.2599328012280203No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC16840.23246247332341274No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC15470.21355074004235125No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG15250.21051381936948005No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT14090.1945009649125229No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA13480.18608041213774368No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT12560.17338056205119143No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA12070.1666165114616147No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA11970.16523609297394598No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT11650.16081875381340607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA11590.15999050272080484No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA11080.15295036843369436No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC10950.15115582439972503No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC10640.14687652708795199No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA10630.14673848523918512No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA10540.14549610860028325No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA9940.13721359767427094No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT9620.132796258513731No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC9290.12824087750442423No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG9260.12782675195812362No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAA8980.1239615801926512No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA8930.12327137094881684No Hit
CCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGC8920.12313332910004998No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT8680.11982032472964503No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA8380.11567906926663886No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC8100.11181389750116646No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA8010.1105715208622646No Hit
TCCTCTGCCTAGGAAAACCAGAGACCTTTGTTCACTTGTTTATCTGCTGA7860.10850089313076151No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7640.10546397245789034No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC7620.10518788876035659No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT7420.10242705178501914No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA7380.10187488438995165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACT256.712071E-10283.351744
CGGGCAA207.527888E-8283.351744
GTATAGT109.537703E-4283.351744
CATTATG400.0247.9327444
CTATCAT20350.0228.3522344
AATCGCA1600.0203.6590444
CATGATA355.0276867E-9202.3940944
CAAACAC150.003210482188.9011544
CAATAGG150.003210482188.9011544
GATAAAT256.5070475E-5170.0110344
ATGATTC603.6379788E-12165.2885144
TATATGC351.2247365E-6161.9152744
TAGAGTC200.007589957141.6758644
CATTACA301.345507E-4141.6758644
CCATCTA701.2732926E-11141.6758644
GTACTTC200.007589957141.6758644
ATTGGAC200.007589957141.6758644
ACTTCTA8100.0136.428644
GCAGCGA454.277779E-6125.934144
TTTTGAC454.277779E-6125.934144
Produced by FastQC (version 0.11.5)