##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841060.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724418
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.07970536347799	39.0	37.0	39.0	33.0	39.0
2	37.70263991231582	39.0	37.0	39.0	35.0	39.0
3	38.04066436780975	39.0	38.0	39.0	35.0	40.0
4	38.71000030369207	39.0	38.0	41.0	35.0	41.0
5	39.17914657007418	40.0	38.0	41.0	35.0	41.0
6	39.132079821318634	40.0	38.0	41.0	35.0	41.0
7	39.15264943720338	40.0	38.0	41.0	35.0	41.0
8	39.09716627692851	40.0	38.0	41.0	35.0	41.0
9	39.08301560701142	40.0	38.0	41.0	35.0	41.0
10	39.01847690145745	40.0	38.0	41.0	35.0	41.0
11	38.934212015714685	40.0	38.0	41.0	35.0	41.0
12	38.87992015659467	40.0	38.0	41.0	35.0	41.0
13	38.7311993904072	40.0	38.0	41.0	35.0	41.0
14	38.70917481343644	40.0	38.0	41.0	35.0	41.0
15	38.6762794408753	40.0	38.0	41.0	35.0	41.0
16	38.65270465394289	40.0	38.0	41.0	35.0	41.0
17	38.65363091474811	40.0	38.0	41.0	35.0	41.0
18	38.59694540997049	40.0	38.0	41.0	35.0	41.0
19	38.55704441358442	40.0	38.0	41.0	35.0	41.0
20	38.36375545610407	40.0	37.0	41.0	34.0	41.0
21	38.30751858733494	40.0	37.0	41.0	34.0	41.0
22	38.26157411880986	40.0	37.0	41.0	34.0	41.0
23	38.16544177532861	40.0	37.0	41.0	34.0	41.0
24	37.89777725015116	39.0	36.0	41.0	33.0	41.0
25	37.83829692747509	39.0	36.0	41.0	33.0	41.0
26	37.69326047969428	39.0	36.0	41.0	33.0	41.0
27	37.608467885405375	39.0	36.0	41.0	33.0	41.0
28	37.40278106550812	39.0	35.0	41.0	33.0	41.0
29	37.380699069688355	39.0	35.0	41.0	33.0	41.0
30	37.285837428676274	38.0	35.0	40.0	33.0	41.0
31	37.24837012939066	38.0	35.0	40.0	33.0	41.0
32	37.15834412779083	38.0	35.0	40.0	32.0	41.0
33	36.96094476774044	38.0	35.0	40.0	32.0	41.0
34	36.89070678697251	38.0	35.0	40.0	32.0	41.0
35	36.70557863351751	38.0	35.0	40.0	32.0	41.0
36	36.59088900236203	37.0	35.0	40.0	31.0	41.0
37	36.51168027188419	37.0	35.0	40.0	31.0	41.0
38	36.226929721981215	37.0	34.0	40.0	31.0	41.0
39	36.17181276714591	37.0	34.0	40.0	31.0	41.0
40	35.98877472143038	36.0	34.0	40.0	31.0	41.0
41	35.86913450949891	36.0	34.0	40.0	31.0	41.0
42	35.78233614513825	36.0	34.0	40.0	30.0	41.0
43	35.55010036604085	36.0	34.0	39.0	30.0	41.0
44	35.18723332259104	35.0	33.0	39.0	29.0	41.0
45	34.892981522187114	35.0	33.0	39.0	29.0	40.0
46	34.60644018002617	35.0	32.0	39.0	28.0	40.0
47	34.84699823574663	35.0	33.0	39.0	29.0	40.0
48	35.57106999551572	36.0	34.0	39.0	31.0	40.0
49	36.085957534106065	37.0	35.0	39.0	32.0	40.0
50	36.364709848206935	37.0	35.0	39.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	7.0
22	26.0
23	52.0
24	87.0
25	158.0
26	323.0
27	590.0
28	1324.0
29	2664.0
30	5317.0
31	10071.0
32	18355.0
33	31436.0
34	48732.0
35	52838.0
36	84459.0
37	122229.0
38	118747.0
39	107467.0
40	119530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	37.36972300522626	19.00781040780323	43.6224665869705
2	18.966121769475635	19.531817265722275	38.42878006896571	23.073280895836383
3	23.85266517397414	23.253287466628382	26.833954981792278	26.060092377605194
4	21.365565184741406	26.244378245708972	30.193203371534118	22.19685319801551
5	15.525428688961346	27.193968123376283	29.669472597312602	27.61113059034977
6	17.461465617916726	27.193553997829984	27.259676043389312	28.085304340863974
7	22.53988719220119	20.402999373290008	22.053841842693032	35.003271591815775
8	21.89178071224072	26.644837648981667	29.20537590175838	22.258005737019236
9	22.597864768683273	29.257969846138554	27.559364897062196	20.584800488115977
10	31.164189735760296	19.8163215160308	25.215138221303167	23.804350526905736
11	23.043601898351504	20.111040863148073	24.423330176776393	32.42202706172404
12	20.01703436413783	26.619575990657328	25.64016907365637	27.723220571548467
13	26.199238561162204	22.729694734255638	23.824918762372	27.246147942210158
14	17.817751629584023	24.84891319652466	21.62618819521326	35.707146978678054
15	23.67362489612351	32.38158080003534	23.148789787111863	20.79600451672929
16	18.644622303697588	34.10406698894837	20.720771709151347	26.530538998202697
17	18.26597351253006	39.67460775408673	18.412435914071708	23.646982819311503
18	18.88246840912291	27.871891642670395	22.30949534660928	30.93614460159742
19	23.99001681349718	24.297021885154706	20.984293598447305	30.728667702900815
20	21.06132095005922	23.12187162660232	27.8089445596327	28.007862863705764
21	22.15654497817558	35.43230565778322	21.081889185525483	21.329260178515717
22	27.607403460433066	24.70189862758794	21.21620390437565	26.474494007603344
23	29.274810951688114	22.550240330858703	26.48705581584113	21.687892901612052
24	22.245858054327748	26.19316471981646	26.12635246501329	25.434624760842496
25	23.036442865469482	24.130006383746426	20.719420466846152	32.11413028393794
26	23.235087072389923	31.589371413885768	24.091005592522446	21.084535921201862
27	23.582298614890664	27.920170224016715	22.342183463349944	26.155347697742677
28	23.10807458698626	22.685244902443	21.35807281515553	32.84860769541521
29	20.47342875064476	31.156652706991128	26.647977561643327	21.721940980720788
30	23.552335244893115	30.31292497427669	25.284539390843218	20.850200389986977
31	25.20070247676629	29.522556135161626	23.3360696917181	21.940671696353984
32	20.360653874566705	30.114569384927197	23.72808749411708	25.796689246389015
33	21.24538899698029	22.68041237113402	30.090444622477335	25.983754009408354
34	24.499873124155393	30.764294186725838	23.778893760659045	20.956938928459728
35	19.12176110821709	23.180662597227975	23.51310397846255	34.18447231609239
36	19.3241778422783	22.477238183115794	31.057151644912672	27.14143232969323
37	19.785158654149264	35.854426674432496	21.66547076854718	22.694943902871056
38	27.751329737070428	22.769323625938362	22.414274397374477	27.065072239616732
39	19.277309597823976	32.84695575092287	26.70882310083544	21.16691155041772
40	19.075335245969256	24.84685163628142	28.53845284252529	27.539360275224034
41	18.36666486981834	33.05127948435587	22.285437057686533	26.29661858813926
42	19.466891735407447	32.791489665956334	20.799584679782203	26.942033918854015
43	19.227771873893023	23.21580666745385	23.41260282599284	34.14381863266029
44	19.823396712858525	25.93103448275862	20.06542056074766	34.180148243635195
45	19.803029262261298	24.849567248248388	35.03795164170903	20.309451847781286
46	19.542736655980626	29.60436882284713	29.244277362354502	21.60861715881774
47	19.572157041582024	30.680273989009116	28.54773848513743	21.199830484271427
48	25.474780299456647	25.72374512989849	19.67928670848111	29.122187862163752
49	15.428932882004991	30.6091223031422	22.742503504632953	31.219441310219853
50	15.883245258325815	30.379433081171115	34.09543827643986	19.641883384063217
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14633.0
1	13482.0
2	9818.5
3	6968.0
4	5989.5
5	4049.0
6	2027.0
7	1327.0
8	1252.0
9	1183.5
10	1199.0
11	1244.0
12	1295.0
13	1193.0
14	1133.0
15	1218.5
16	1367.5
17	1351.5
18	1108.5
19	1056.5
20	1198.0
21	1356.0
22	1544.5
23	1774.5
24	2055.0
25	2541.5
26	3317.5
27	4210.5
28	5258.5
29	6439.5
30	7810.5
31	11721.0
32	18211.5
33	20878.0
34	20903.0
35	22327.0
36	22528.0
37	21565.5
38	21656.0
39	24098.0
40	26552.5
41	30035.0
42	36182.0
43	40316.5
44	41376.0
45	41583.0
46	43727.5
47	46742.5
48	54450.5
49	71283.5
50	92869.0
51	104873.0
52	96156.0
53	76903.5
54	62792.5
55	57738.5
56	54414.0
57	50278.0
58	47681.5
59	43948.5
60	38185.5
61	32770.5
62	27600.0
63	21337.5
64	15902.5
65	13000.0
66	9221.5
67	5849.5
68	4259.5
69	2941.0
70	2075.5
71	1521.5
72	1110.0
73	699.0
74	433.0
75	265.5
76	165.5
77	103.0
78	45.5
79	25.0
80	18.5
81	14.5
82	11.5
83	9.5
84	7.5
85	5.5
86	2.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	3838.0
25	4624.0
26	4893.0
27	5578.0
28	5612.0
29	4978.0
30	4196.0
31	4390.0
32	4787.0
33	3694.0
34	4025.0
35	4041.0
36	3596.0
37	3441.0
38	3909.0
39	4222.0
40	5309.0
41	5926.0
42	8184.0
43	14575.0
44	38280.0
45	71061.0
46	77081.0
47	90756.0
48	109446.0
49	133115.0
50	100861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.372163585112464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.18436066130951	7.5265937621649375
2	12.036609939064359	2.737645944744609
3	4.777742710786336	1.6299981502392265
4	2.3403170595518437	1.0645787376901181
5	1.648418343812969	0.937304153127062
6	1.1264596635187298	0.7686170139339442
7	0.8703357529557427	0.6928320389609314
8	0.7186035784516035	0.6537661957599066
9	0.5826515500958948	0.5963407866728877
>10	5.584957879148358	14.803055694364303
>50	2.3148260542351484	18.732140835815787
>100	1.7236775023670219	32.58519252696648
>500	0.06190672719768881	4.7781805532165125
>1k	0.021849433128596052	3.578596887432394
>5k	0.003641572188099342	2.8345513225789527
>10k+	0.003641572188099342	6.080605396331952
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	17646	2.435886463340226	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	15069	2.0801526190679964	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	11334	1.5645663139237294	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	8495	1.1726655052745791	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	6605	0.9117664111051906	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	5434	0.7501194061991834	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3119	0.4305525263038743	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	2041	0.2817434133331861	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	1883	0.2599328012280203	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	1684	0.23246247332341274	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC	1547	0.21355074004235125	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1525	0.21051381936948005	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	1409	0.1945009649125229	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	1348	0.18608041213774368	No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT	1256	0.17338056205119143	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	1207	0.1666165114616147	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	1197	0.16523609297394598	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	1165	0.16081875381340607	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	1159	0.15999050272080484	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	1108	0.15295036843369436	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	1095	0.15115582439972503	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	1064	0.14687652708795199	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	1063	0.14673848523918512	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	1054	0.14549610860028325	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	994	0.13721359767427094	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	962	0.132796258513731	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	929	0.12824087750442423	No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG	926	0.12782675195812362	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAA	898	0.1239615801926512	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	893	0.12327137094881684	No Hit
CCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGC	892	0.12313332910004998	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	868	0.11982032472964503	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	838	0.11567906926663886	No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC	810	0.11181389750116646	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	801	0.1105715208622646	No Hit
TCCTCTGCCTAGGAAAACCAGAGACCTTTGTTCACTTGTTTATCTGCTGA	786	0.10850089313076151	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	764	0.10546397245789034	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	762	0.10518788876035659	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	742	0.10242705178501914	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	738	0.10187488438995165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.043759266059098474	0.0	0.0	0.0	0.0
2	0.08600007178176136	0.0	0.0	0.0	0.0
3	0.11995836657841191	0.0	0.0	0.0	0.0
4	0.14452981565891515	0.0	0.0	0.0	0.0
5	0.1656502185202466	0.0	0.0	0.0	0.0
6	0.1968476763415597	0.0	0.0	0.0	0.0
7	0.21907241399302613	0.0	0.0	0.0	0.0
8	0.2604849686230878	0.0	0.0	0.0	0.0
9	0.29030200795673217	0.0	0.0	0.0	0.0
10	0.31514954073476914	0.0	0.0	0.0	0.0
11	0.3416535756980086	0.0	0.0	0.0	0.0
12	0.3778205400749291	0.0	0.0	0.0	0.0
13	0.40846583050117474	0.0	0.0	0.0	0.0
14	0.43013840075757365	0.0	0.0	0.0	0.0
15	0.4549859335356107	0.0	0.0	0.0	0.0
16	0.481628010347617	0.0	0.0	0.0	0.0
17	0.5182091002708381	0.0	0.0	0.0	0.0
18	0.5730117142312864	0.0	0.0	0.0	0.0
19	0.5942701589413847	0.0	0.0	0.0	0.0
20	0.6184274824755873	0.0	0.0	0.0	0.0
21	0.6424467641610231	0.0	0.0	0.0	0.0
22	0.6623247903834526	0.0	0.0	0.0	0.0
23	0.682478900303416	0.0	0.0	0.0	0.0
24	0.702909093920913	0.0	0.0	0.0	0.0
25	0.724581664177312	0.0	0.0	0.0	0.0
26	0.7487389877115146	0.0	0.0	0.0	0.0
27	0.775381064523521	0.0	0.0	0.0	0.0
28	0.8057502712522329	0.0	0.0	0.0	0.0
29	0.8358433942834109	0.0	0.0	0.0	0.0
30	0.8593105085737792	0.0	0.0	0.0	0.0
31	0.8836058739567487	0.0	0.0	0.0	0.0
32	0.9069349463983502	0.0	0.0	0.0	0.0
33	0.9302640188399515	0.0	0.0	0.0	0.0
34	0.9573202211982584	0.0	0.0	0.0	0.0
35	0.9763699963280869	0.0	0.0	0.0	0.0
36	1.0002512361647558	0.0	0.0	0.0	0.0
37	1.0217857645723878	0.0	0.0	0.0	0.0
38	1.0329671543225043	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATACT	25	6.712071E-10	283.3517	44
CGGGCAA	20	7.527888E-8	283.3517	44
GTATAGT	10	9.537703E-4	283.3517	44
CATTATG	40	0.0	247.93274	44
CTATCAT	2035	0.0	228.35223	44
AATCGCA	160	0.0	203.65904	44
CATGATA	35	5.0276867E-9	202.39409	44
CAAACAC	15	0.003210482	188.90115	44
CAATAGG	15	0.003210482	188.90115	44
GATAAAT	25	6.5070475E-5	170.01103	44
ATGATTC	60	3.6379788E-12	165.28851	44
TATATGC	35	1.2247365E-6	161.91527	44
TAGAGTC	20	0.007589957	141.67586	44
CATTACA	30	1.345507E-4	141.67586	44
CCATCTA	70	1.2732926E-11	141.67586	44
GTACTTC	20	0.007589957	141.67586	44
ATTGGAC	20	0.007589957	141.67586	44
ACTTCTA	810	0.0	136.4286	44
GCAGCGA	45	4.277779E-6	125.9341	44
TTTTGAC	45	4.277779E-6	125.9341	44
>>END_MODULE