FastQCFastQC Report
Tue 24 May 2016
ERR841059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649158
Sequences flagged as poor quality0
Sequence length24-50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA150582.319620184916461No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA145152.23597336857899No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA139612.1506320495164504No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA117301.8069560877321083No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA114071.757199325896007No Hit
AAAAAAAAAAAAAAAAAAAAAAAA90201.389492234556148No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73061.125457900850024No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC71551.1021969998059025No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA46620.7181610640244748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41630.6412922585872777No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT30740.47353648880549876No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA30590.4712258032713145No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA30290.466604432202946No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT27400.4220852242443288No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA26210.4037537856731335No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24240.37340678232417995No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21970.3384384079068578No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA21200.3265768888313785No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC17970.2768201269952769No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA15970.24601098653948655No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15780.24308411819618644No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA12680.19532995048971127No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA11930.18377652281878987No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAA11860.18269820290283723No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAG11420.1759201920025633No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA11420.1759201920025633No Hit
TAAAAAAAAAAAAAAAAAAAAAAA11300.1740716435752159No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11090.1708366838273579No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA11070.1705285924228No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAG10800.1663693584612683No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAG10710.16498294714075773No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA9850.15173501674476783No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9710.1495783769128625No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAG9630.14834601129463088No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT9320.14357059452398338No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG9020.1389492234556148Illumina Single End Adapter 1 (100% over 32bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9010.13879517775333586No Hit
AAAAAAAAAAAAAAAAAAAAAAAAG8480.13063075553255138No Hit
ACAAGTAGCATCCGTACTATACTTCACAACAATCCTAATCCTAATACCA8380.12909029850976186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAG8220.12662556727329866No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC8200.12631747586874073No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC8020.1235446532277196No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA7760.11953946496846685No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA7130.10983458572489287No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6880.10598344316791906No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6510.10028375218359782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATATG301.6370905E-11244.4044344
GTTCTAA151.5297821E-5244.4044344
AGTCACT151.5297821E-5244.4044344
CGTGCGC151.5297821E-5244.4044344
ACACCTG301.6370905E-11244.4044344
ATCAGAT301.6370905E-11244.4044344
TCCTAAC151.5297821E-5244.4044344
TAGCAAG151.5297821E-5244.4044344
GGTAGCC151.5297821E-5244.4044344
TCACGAC100.0014848562244.4044244
CACGGCT201.573244E-7244.4044244
GAGTCAT251.6261765E-9244.4044244
TAAACGC201.573244E-7244.4044244
ATAGAAC201.573244E-7244.4044244
CTTACGA400.0244.4044244
CGTATCG254.784888E-7195.5235344
CTATCAT4300.0190.4080844
CATAAGC451.8189894E-12190.0923344
CATTATG204.8191076E-5183.3033144
AAACGGC351.2159944E-8174.5745844
Produced by FastQC (version 0.11.5)