##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841059.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	649158
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.09673762011714	39.0	35.0	39.0	31.0	39.0
2	37.06691283169891	39.0	37.0	39.0	33.0	39.0
3	37.34286106001929	39.0	37.0	39.0	34.0	39.0
4	37.84524260657652	39.0	37.0	39.0	35.0	41.0
5	38.59264616626461	40.0	37.0	41.0	35.0	41.0
6	38.52611536790735	40.0	37.0	41.0	35.0	41.0
7	38.577526580585925	40.0	37.0	41.0	35.0	41.0
8	38.55119708915241	40.0	37.0	41.0	35.0	41.0
9	38.55892094066468	40.0	37.0	41.0	35.0	41.0
10	38.497091617140974	40.0	37.0	41.0	35.0	41.0
11	38.456488250934285	40.0	37.0	41.0	35.0	41.0
12	38.419708299058165	40.0	37.0	41.0	34.0	41.0
13	38.19625730561743	40.0	36.0	41.0	34.0	41.0
14	37.941125273046005	39.0	36.0	40.0	34.0	41.0
15	37.486383900375564	38.0	35.0	40.0	34.0	41.0
16	37.23758622708185	38.0	35.0	40.0	33.0	41.0
17	37.16872163633507	38.0	35.0	40.0	33.0	41.0
18	37.101315858388865	38.0	35.0	40.0	33.0	41.0
19	37.13627036869298	38.0	35.0	40.0	33.0	41.0
20	37.00930744133169	38.0	35.0	40.0	33.0	41.0
21	36.9038323489813	38.0	35.0	40.0	33.0	41.0
22	36.79376207333192	38.0	35.0	40.0	33.0	41.0
23	36.74102606761374	38.0	35.0	40.0	33.0	41.0
24	36.35389689413055	37.0	35.0	40.0	32.0	41.0
25	36.2501777435165	37.0	35.0	40.0	31.0	41.0
26	36.08640501344977	37.0	35.0	40.0	31.0	41.0
27	36.015234213828	37.0	35.0	40.0	31.0	41.0
28	35.898786093851044	37.0	34.0	40.0	30.0	41.0
29	35.94357459433855	37.0	34.0	40.0	30.0	41.0
30	36.07670919577156	37.0	35.0	40.0	31.0	41.0
31	36.22284294611213	37.0	35.0	40.0	31.0	41.0
32	36.29576739867593	37.0	35.0	40.0	31.0	41.0
33	36.242770549240866	37.0	35.0	40.0	31.0	41.0
34	36.183235538558634	37.0	34.0	40.0	31.0	41.0
35	36.013742549347384	36.0	34.0	40.0	31.0	41.0
36	35.931548248339965	36.0	34.0	40.0	31.0	41.0
37	35.94413660766262	36.0	34.0	40.0	31.0	41.0
38	35.741060518606446	36.0	34.0	40.0	31.0	41.0
39	35.67352752119894	36.0	34.0	39.0	30.0	41.0
40	35.48801580372733	35.0	34.0	39.0	30.0	41.0
41	35.324695703612726	35.0	34.0	39.0	30.0	41.0
42	35.153687393341045	35.0	33.0	39.0	29.0	41.0
43	35.015622815601844	35.0	33.0	39.0	29.0	41.0
44	34.687570374301636	35.0	33.0	38.0	29.0	40.0
45	34.4564049889208	35.0	33.0	38.0	28.0	40.0
46	34.20189637172318	35.0	32.0	38.0	27.0	40.0
47	34.074473625980836	35.0	32.0	37.0	28.0	40.0
48	34.942272244179485	35.0	33.0	38.0	30.0	40.0
49	35.463381810025524	35.0	34.0	39.0	31.0	40.0
50	35.43169965374257	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	31.0
22	62.0
23	135.0
24	273.0
25	485.0
26	865.0
27	1478.0
28	2756.0
29	4760.0
30	8631.0
31	14712.0
32	25979.0
33	44496.0
34	63753.0
35	53157.0
36	101473.0
37	124318.0
38	78916.0
39	69363.0
40	53505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.62386044691739	18.511826088563954	28.864313464518652
2	18.59331626506952	39.69295610621759	25.789869338435327	15.92385829027756
3	17.227238977259773	39.80402305756072	21.350272198755928	21.618465766423583
4	15.771353045021396	42.292631377877186	26.652679316899736	15.283336260201677
5	14.871572098010038	41.96944349449595	24.84279636082433	18.316188046669684
6	15.886425184623773	43.02604296642728	20.97332236527933	20.114209483669615
7	20.359758333102267	40.60321216098392	18.118085273538952	20.918944232374862
8	20.79231866510156	39.387637524300715	23.310349714553315	16.509694096044413
9	19.001383330406465	44.83392332837306	19.959547598581548	16.205145742638926
10	21.856466376444565	39.84207234602361	19.017558129145755	19.283903148386063
11	19.209807165589886	38.889761814535134	21.11473631997141	20.785694699903566
12	19.4256251944827	42.62829696314302	18.370104042467318	19.575973799906958
13	18.07418224838945	39.248534255142815	18.625511816845822	24.05177167962191
14	18.255031902864943	39.82558945587977	20.307844931434257	21.61153370982103
15	18.067558283191456	44.28906367941241	20.66723355485105	16.97614448254508
16	16.794370553855916	47.23503368979509	17.79520548156227	18.175390274786725
17	20.103888421616926	45.690725524448595	16.306661860440755	17.898724193493727
18	18.99753218784949	42.44005311495815	17.370655526081478	21.191759171110885
19	19.559644955465387	41.10940633867256	17.626371391864538	21.704577313997518
20	19.54809152779447	40.66051716223169	19.16374750060848	20.627643809365363
21	20.046737466071434	46.251914017850815	17.452145702587043	16.249202813490708
22	20.22373597798995	43.59739847617991	16.031998373277386	20.146867172552753
23	20.15811250881912	41.38530219145416	20.331567969585215	18.125017330141507
24	20.107277427067064	42.07172984080917	19.207496480055703	18.613496252068064
25	19.91909714175814	41.21253395255864	18.66267512268638	20.205693782996843
26	19.58695567424272	45.75243980091744	17.860089925019167	16.80051459982067
27	21.760371845502235	40.344058134541385	18.44087605991967	19.45469396003671
28	21.899337869373294	36.7849762420221	18.68990215649338	22.625783732111227
29	22.049843390261188	36.46454891621654	23.197152016097938	18.288455677424327
30	22.021050377101595	35.08937996236633	24.322288004650662	18.567281655881406
31	21.525152144227203	35.13604180315576	21.995398179794616	21.34340787282242
32	21.853907637655418	32.040408525754884	23.186864201517842	22.918819635071856
33	21.014138140904382	31.28073955883079	27.725025907588048	19.98009639267679
34	21.594419344827084	34.533532463907584	23.495741396871818	20.376306794393518
35	20.78668760447799	30.280676426388396	23.569812403964676	25.36282356516894
36	21.376809272702808	29.837488493484805	24.665269866492388	24.120432367320003
37	20.773808240443284	36.68463379401048	20.88958840904681	21.651969556499427
38	23.71453775197935	30.35470415908868	22.236258889385013	23.694499199546954
39	18.023941814083315	37.35085896431182	24.010496006762526	20.61470321484234
40	20.10543241991234	31.93150233403414	23.21896269108791	24.744102554965615
41	19.46706644961583	36.86981691416031	22.34735508808847	21.315761548135388
42	19.304784047829024	34.56906918944921	23.03054597931532	23.095600783406447
43	23.99482734516986	28.677477981266602	20.55990493499231	26.767789738571228
44	21.33091401126945	30.67040561894721	20.886016940035717	27.11266342974762
45	20.66990041338791	30.566225349411212	28.02734720041995	20.736527036780924
46	20.95390643994108	31.62621741058021	25.527508831142832	21.892367318335886
47	18.675426801245937	34.59175162975949	24.511582781569675	22.221238787424895
48	23.979956159287923	29.566740841608823	22.24417948121824	24.20912351788502
49	19.536975854606293	32.081911262798634	20.956722579550068	27.424390303044998
50	20.936818706450648	30.798743213781897	26.980293250117555	21.284144829649897
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	121334.0
1	117874.0
2	71451.0
3	28098.5
4	26843.0
5	19432.0
6	8232.0
7	3623.5
8	3305.0
9	2706.0
10	2315.0
11	2046.0
12	1786.5
13	1604.0
14	1668.0
15	1812.0
16	1919.0
17	2126.0
18	2423.5
19	2727.5
20	2970.0
21	3369.5
22	4000.0
23	4902.0
24	6123.5
25	7532.0
26	8573.5
27	8566.0
28	8287.5
29	8762.0
30	9651.5
31	10772.5
32	12264.5
33	13612.5
34	16476.5
35	20396.5
36	22217.5
37	21989.5
38	21810.5
39	22446.0
40	23446.5
41	23728.5
42	23219.0
43	23303.5
44	24877.0
45	27498.0
46	29999.0
47	30727.5
48	32920.0
49	38858.5
50	46737.5
51	53742.5
52	53809.0
53	49064.0
54	45057.5
55	41211.0
56	38001.5
57	36732.0
58	35584.5
59	31594.5
60	26890.0
61	23603.5
62	19314.0
63	14266.0
64	10991.5
65	9422.5
66	7512.5
67	5864.5
68	4622.0
69	3382.5
70	2490.0
71	1630.0
72	921.0
73	557.0
74	378.0
75	334.5
76	313.0
77	271.5
78	198.5
79	98.5
80	40.5
81	33.0
82	30.0
83	20.5
84	8.5
85	8.0
86	5.0
87	2.5
88	3.0
89	3.0
90	2.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	14817.0
25	18717.0
26	21829.0
27	24723.0
28	24406.0
29	21730.0
30	16180.0
31	11316.0
32	9096.0
33	6257.0
34	6303.0
35	5571.0
36	4403.0
37	4695.0
38	4847.0
39	5260.0
40	6101.0
41	6352.0
42	7375.0
43	10881.0
44	22061.0
45	46623.0
46	55860.0
47	52883.0
48	75913.0
49	71387.0
50	93572.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.828001811577458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.45353891971084	8.781221212709386
2	11.426135408410788	2.9314897143684586
3	4.563249033311718	1.756121005980054
4	2.440137377813003	1.2520834681233228
5	1.5539063813435166	0.9966756937448202
6	1.0723635228282538	0.8253768728106254
7	0.820183970987343	0.7364925025956701
8	0.7012993251194851	0.7197015210472643
9	0.5235727838220813	0.604475335742608
>10	5.345005644018541	15.676306846715281
>50	1.8937483488243632	17.218150280825313
>100	1.1276028532315008	23.75769843397139
>500	0.042029925306818455	3.6952483062675032
>1k	0.02761966520162356	7.161430653246205
>5k	0.0036025650262987248	3.6171471352120745
>10k+	0.006004275043831208	10.270381016640016
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	15058	2.319620184916461	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	14515	2.23597336857899	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	13961	2.1506320495164504	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11730	1.8069560877321083	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	11407	1.757199325896007	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	9020	1.389492234556148	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7306	1.125457900850024	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	7155	1.1021969998059025	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	4662	0.7181610640244748	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4163	0.6412922585872777	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	3074	0.47353648880549876	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	3059	0.4712258032713145	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3029	0.466604432202946	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	2740	0.4220852242443288	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	2621	0.4037537856731335	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2424	0.37340678232417995	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2197	0.3384384079068578	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA	2120	0.3265768888313785	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	1797	0.2768201269952769	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA	1597	0.24601098653948655	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1578	0.24308411819618644	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA	1268	0.19532995048971127	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1193	0.18377652281878987	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAA	1186	0.18269820290283723	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAG	1142	0.1759201920025633	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	1142	0.1759201920025633	No Hit
TAAAAAAAAAAAAAAAAAAAAAAA	1130	0.1740716435752159	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1109	0.1708366838273579	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	1107	0.1705285924228	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAG	1080	0.1663693584612683	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAG	1071	0.16498294714075773	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA	985	0.15173501674476783	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	971	0.1495783769128625	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAG	963	0.14834601129463088	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	932	0.14357059452398338	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	902	0.1389492234556148	Illumina Single End Adapter 1 (100% over 32bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	901	0.13879517775333586	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAG	848	0.13063075553255138	No Hit
ACAAGTAGCATCCGTACTATACTTCACAACAATCCTAATCCTAATACCA	838	0.12909029850976186	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAG	822	0.12662556727329866	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	820	0.12631747586874073	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	802	0.1235446532277196	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA	776	0.11953946496846685	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	713	0.10983458572489287	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	688	0.10598344316791906	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	651	0.10028375218359782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.39035180957486465	0.0	0.0	0.0	0.0
2	0.7295604459931172	0.0	0.0	0.0	0.0
3	0.9723364727847458	0.0	0.0	0.0	0.0
4	1.1222229411021662	0.0	0.0	0.0	0.0
5	1.2553184278711809	0.0	0.0	0.0	0.0
6	1.4323169397896969	0.0	0.0	0.0	0.0
7	1.5671069292837798	0.0	0.0	0.0	0.0
8	1.6917299024274521	0.0	0.0	0.0	0.0
9	1.8151205099528929	0.0	0.0	0.0	0.0
10	1.9608477443087815	0.0	0.0	0.0	0.0
11	2.1119665782444335	0.0	0.0	0.0	0.0
12	2.25661549268437	0.0	0.0	0.0	0.0
13	2.404037229765327	0.0	0.0	0.0	0.0
14	2.520341734985936	0.0	0.0	0.0	0.0
15	2.608918013796333	0.0	0.0	0.0	0.0
16	2.6820897223788354	0.0	0.0	0.0	0.0
17	2.7484834200610635	0.0	0.0	0.0	0.0
18	2.795775450660702	0.0	0.0	0.0	0.0
19	2.8364435160623453	0.0	0.0	0.0	0.0
20	2.8744928045252465	0.0	0.0	0.0	0.0
21	2.9159310984382847	0.0	0.0	0.0	0.0
22	2.9512075642601645	0.0	0.0	0.0	0.0
23	2.9847895273569764	0.0	0.0	0.0	0.0
24	3.020836221690251	0.0	0.0	0.0	0.0
25	3.052261544955157	0.0	0.0	0.0	0.0
26	3.094161975975032	0.0	0.0	0.0	0.0
27	3.1285141675832384	0.0	0.0	0.0	0.0
28	3.1607097193595397	0.0	0.0	0.0	0.0
29	3.1933674082426773	0.0	0.0	0.0	0.0
30	3.2315707424078575	0.0	0.0	0.0	0.0
31	3.2649986598023903	0.0	0.0	0.0	0.0
32	3.2998129885174334	0.0	0.0	0.0	0.0
33	3.3323166316982924	0.0	0.0	0.0	0.0
34	3.361739360833572	0.0	0.0	0.0	0.0
35	3.3860785817936465	0.0	0.0	0.0	0.0
36	3.406874751601305	0.0	0.0	0.0	0.0
37	3.4248980987679425	0.0	0.0	0.0	0.0
38	3.4338327495001217	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATATG	30	1.6370905E-11	244.40443	44
GTTCTAA	15	1.5297821E-5	244.40443	44
AGTCACT	15	1.5297821E-5	244.40443	44
CGTGCGC	15	1.5297821E-5	244.40443	44
ACACCTG	30	1.6370905E-11	244.40443	44
ATCAGAT	30	1.6370905E-11	244.40443	44
TCCTAAC	15	1.5297821E-5	244.40443	44
TAGCAAG	15	1.5297821E-5	244.40443	44
GGTAGCC	15	1.5297821E-5	244.40443	44
TCACGAC	10	0.0014848562	244.40442	44
CACGGCT	20	1.573244E-7	244.40442	44
GAGTCAT	25	1.6261765E-9	244.40442	44
TAAACGC	20	1.573244E-7	244.40442	44
ATAGAAC	20	1.573244E-7	244.40442	44
CTTACGA	40	0.0	244.40442	44
CGTATCG	25	4.784888E-7	195.52353	44
CTATCAT	430	0.0	190.40808	44
CATAAGC	45	1.8189894E-12	190.09233	44
CATTATG	20	4.8191076E-5	183.30331	44
AAACGGC	35	1.2159944E-8	174.57458	44
>>END_MODULE