FastQCFastQC Report
Tue 24 May 2016
ERR841058.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841058.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2277866
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC565202.481269749844811No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA368871.6193665474615277No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT212650.9335492078989722No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA127600.5601734254780572No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA126550.5555638479173051No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA114170.5012147334391048No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA102480.44989476992939886No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT98860.4340027025294728No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA91120.4000235307959291No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68180.2993152362781656No Hit
AAAAAAAAAAAAAAAAAAAAAAAA67400.29589097866160696No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC57470.25229754515849484No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC55350.24299058855964312No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC54560.23952243020441064No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA50860.22327915689509392No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC46040.20211900085430837No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG45560.20001176539796456No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39590.17380302440968873No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT38900.17077387344119452No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35420.15549641638270206No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA34860.15303797501696761No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG33020.14496023910098313No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC31360.13767271648112753No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG30730.1349069699446763No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT30600.1343362603419165No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC30390.1334143448297661No Hit
CCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGC29740.13056079681596722No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAA27940.12265866385467801No Hit
ATGTAAGCCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAG27440.12046362692098658No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC26500.11633695748564665No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTT25730.11295660060776183No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG25250.11084936515141804No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCTT24810.10891773264976956No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC24610.10803971787629299No Hit
CCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGCC24330.10681049719342578No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT24060.1056251772492324No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT23380.10263992701941202No Hit
CTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAA23050.10119120264317567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAC251.5788828E-9245.8595744
AACGTAT151.4974377E-5245.8595644
ACGAACA1700.0173.5479444
CTATCAT17000.0154.746944
TTATGCA251.1479436E-4147.5157544
GAACGTA152.473772E-4121.6602243
TCGTAAC152.473772E-4121.6602243
ATATTGG152.473772E-4121.6602243
ACTTCTA12200.0117.8916844
GCTTCAG7200.0110.9782844
AATCGCA3000.0106.53915444
ACATTGG4650.0105.74605644
CCGGCTA354.3813727E-4105.3683944
CGACCAG354.3813727E-4105.3683944
GACTCAA303.1355557E-7101.38351443
TCGCTCC1100.0100.5789244
AGATATG759.669748E-998.34383444
GATTCAA801.513763E-892.1973444
CGAGAAA4650.089.8841544
CGCCTAC852.305751E-886.7739744
Produced by FastQC (version 0.11.5)