##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841058.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2277866
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.22691413805729	39.0	35.0	39.0	31.0	39.0
2	37.067280077054576	39.0	37.0	39.0	33.0	39.0
3	37.32090957062444	39.0	37.0	39.0	33.0	39.0
4	37.96653622293849	39.0	37.0	40.0	34.0	41.0
5	38.61143544001271	40.0	37.0	41.0	35.0	41.0
6	38.554404429408926	40.0	37.0	41.0	35.0	41.0
7	38.57122280239487	40.0	37.0	41.0	35.0	41.0
8	38.52869483981937	40.0	37.0	41.0	35.0	41.0
9	38.550967440578155	40.0	37.0	41.0	35.0	41.0
10	38.48789129825899	40.0	37.0	41.0	35.0	41.0
11	38.44452570958959	40.0	37.0	41.0	34.0	41.0
12	38.43858418361747	40.0	37.0	41.0	34.0	41.0
13	38.34977869637635	40.0	36.0	41.0	34.0	41.0
14	38.2793860569498	40.0	36.0	41.0	34.0	41.0
15	38.16479590985598	40.0	36.0	41.0	34.0	41.0
16	38.04315662115331	40.0	36.0	41.0	34.0	41.0
17	37.92792508426747	40.0	35.0	41.0	34.0	41.0
18	37.88692969647907	39.0	35.0	41.0	34.0	41.0
19	37.92879607492276	40.0	35.0	41.0	34.0	41.0
20	37.86113625647865	39.0	35.0	41.0	34.0	41.0
21	37.76919230542973	39.0	35.0	41.0	34.0	41.0
22	37.62229428772368	39.0	35.0	41.0	33.0	41.0
23	37.54807701594387	39.0	35.0	41.0	33.0	41.0
24	37.345820166770125	39.0	35.0	41.0	33.0	41.0
25	37.28713254688424	38.0	35.0	41.0	33.0	41.0
26	37.176970160604924	38.0	35.0	40.0	33.0	41.0
27	37.065723080929345	38.0	35.0	40.0	33.0	41.0
28	36.961783941303594	38.0	35.0	40.0	33.0	41.0
29	36.87230161821564	38.0	35.0	40.0	32.0	41.0
30	36.84027786598696	38.0	35.0	40.0	32.0	41.0
31	36.79570232497096	38.0	35.0	40.0	32.0	41.0
32	36.67535297637284	38.0	35.0	40.0	32.0	41.0
33	36.56353905633497	38.0	35.0	40.0	31.0	41.0
34	36.529314829860844	37.0	35.0	40.0	32.0	41.0
35	36.365176807272874	37.0	34.0	40.0	31.0	41.0
36	36.31830811016727	37.0	34.0	40.0	31.0	41.0
37	36.31588782348511	37.0	34.0	40.0	31.0	41.0
38	36.12523107026338	36.0	34.0	40.0	31.0	41.0
39	36.00420483010691	36.0	34.0	40.0	31.0	41.0
40	35.78369977560191	36.0	34.0	40.0	31.0	41.0
41	35.695490017864415	36.0	34.0	39.0	30.0	41.0
42	35.58101820723128	35.0	34.0	39.0	30.0	41.0
43	35.33015062894505	35.0	34.0	39.0	30.0	41.0
44	35.11130219754785	35.0	33.0	39.0	29.0	41.0
45	34.92432301011179	35.0	33.0	39.0	29.0	41.0
46	34.55605548068546	35.0	33.0	39.0	28.0	40.0
47	34.566365899686836	35.0	33.0	38.0	28.0	40.0
48	35.33620216129758	35.0	34.0	39.0	30.0	40.0
49	35.868661639642454	36.0	34.0	39.0	31.0	41.0
50	36.059865097414274	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	6.0
21	25.0
22	80.0
23	198.0
24	437.0
25	1016.0
26	1803.0
27	3583.0
28	7192.0
29	13439.0
30	24981.0
31	46926.0
32	86186.0
33	145821.0
34	220781.0
35	144335.0
36	243263.0
37	373580.0
38	358289.0
39	298302.0
40	307622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.0327622432575	20.80218941763914	37.16504833910335
2	20.439481514715965	21.349236522253722	36.34471913624419	21.866562826786122
3	19.138658727071743	29.670050828275237	26.94333204850505	24.247958396147972
4	18.912482121424176	29.43926464506692	32.21532785510649	19.432925378402416
5	16.036720333856337	30.359687532102413	31.76025279801358	21.843339336027668
6	19.714153510346964	25.383407101207883	27.30981541495417	27.592623973490976
7	23.11720707012616	25.229930118804177	22.745894622422917	28.906968188646744
8	22.1485811720268	26.887402507434587	30.102648707167145	20.861367613371463
9	23.03300545334976	31.735975689526953	22.953984123736866	22.277034733386426
10	27.751632448967584	23.687521566237873	23.6987162545997	24.86212973019484
11	23.480222278220054	24.432209796362034	25.05511737740499	27.03245054801292
12	22.68092152918565	27.66176763690226	22.827945103004303	26.82936573090779
13	23.608763641057024	25.35070105089588	22.664546553660312	28.375988754386782
14	19.378093355798804	26.14925548737283	22.863372999114084	31.609278157714282
15	22.351226981745196	35.25277606321004	21.858572892347485	20.53742406269728
16	20.86031399564329	35.63594171035522	21.396034709680027	22.107709584321466
17	21.099748624370353	36.98597722605281	19.995030436382123	21.91924371319472
18	20.223797185611446	27.950239390727987	20.72167546291134	31.10428796074923
19	22.514274325179795	27.125388411785416	20.75218647628965	29.608150786745135
20	21.614879891969064	26.698102522273036	23.637650327104403	28.049367258653497
21	24.059975433146636	34.42792508426747	21.10914338244655	20.402956100139342
22	23.691867739366582	27.85905755650245	21.371801501932072	27.0772732021989
23	25.84159911074664	25.93664420997548	26.617720269761257	21.604036409516628
24	23.018518209587395	28.43503524790308	22.39323998865605	26.153206553853476
25	24.35856130797908	27.24167200682596	21.96673051254908	26.433036172645874
26	23.715388526994335	36.215109249059616	21.140043808653953	18.929458415292096
27	25.883656574817227	28.149202705164793	20.86678659280615	25.100354127211826
28	25.70106899795984	25.848286387750637	20.541681209766	27.908963404523522
29	22.475552923655584	28.951576242619858	27.67453664667642	20.89833418704814
30	25.902779835992074	28.0998236286765	26.459650296453425	19.537746238877997
31	24.94123733743677	32.59743094346944	21.327553518840904	21.133778200252884
32	21.88813667083807	26.81472738151605	23.847814624617154	27.449321323028723
33	23.552581415705856	24.439365541950274	32.040005561172165	19.968047481171702
34	24.220311358509004	31.411393719964614	23.735209690523963	20.633085231002422
35	22.078661706310694	25.260519422191578	23.871672164432752	28.789146707064972
36	22.100688174383688	26.65189062430428	23.562823741274137	27.684597460037896
37	22.12289957743906	35.57242348082895	20.23100728194606	22.073669659785928
38	30.18794048551292	27.108171926289838	20.355713018243286	22.348174569953958
39	22.37145154407455	34.493781028024465	22.174224028263563	20.960543399637423
40	20.448663913114036	28.723057348948444	22.71808101223837	28.110197725699155
41	20.87023403569904	35.971190863611305	21.217503533578892	21.941071567110768
42	22.2130090815491	34.99618119068968	20.88076124887266	21.91004847888855
43	22.208112343868905	25.777695211892016	22.726435479847552	29.28775696439153
44	21.962506149753633	26.474460624890327	22.2055358009859	29.357497424370145
45	21.966819395911898	25.28937798182167	31.190872458458763	21.55293016380767
46	20.711788092158834	28.32976675482783	28.197007181056616	22.761437971956724
47	22.486422992549247	32.86626050098152	24.565714081919605	20.081602424549626
48	27.740636701455003	24.829687056785527	23.10421605765737	24.325460184102102
49	18.181855202698575	26.056048378886498	22.840970021108074	32.92112639730686
50	21.36335718198569	28.200063079962153	27.267973639215814	23.168606098836342
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	123965.0
1	113786.0
2	70144.0
3	35263.0
4	31447.0
5	22159.5
6	10900.0
7	6548.5
8	6223.5
9	5574.0
10	4990.5
11	4602.5
12	4316.5
13	4181.5
14	4526.5
15	4885.0
16	5026.0
17	5406.0
18	6057.5
19	6722.0
20	7251.5
21	8105.0
22	9374.5
23	11683.5
24	14152.5
25	15376.0
26	16181.5
27	17921.5
28	20494.5
29	23113.0
30	26593.5
31	37860.5
32	56067.5
33	61486.5
34	59952.0
35	63999.5
36	64137.5
37	62413.0
38	63762.0
39	68159.0
40	74843.5
41	85158.5
42	95876.5
43	99694.0
44	99656.5
45	103532.0
46	115067.5
47	127341.5
48	156374.5
49	193000.5
50	234702.5
51	270101.5
52	245131.0
53	205529.0
54	192943.5
55	184543.0
56	176209.0
57	172294.0
58	168584.5
59	150217.5
60	125730.5
61	105038.5
62	88725.5
63	78859.0
64	70523.0
65	61782.5
66	48323.5
67	35497.0
68	26128.0
69	18772.0
70	14202.5
71	10139.5
72	6911.5
73	4698.0
74	3220.5
75	2303.5
76	1994.5
77	1619.5
78	1251.5
79	1024.0
80	612.0
81	335.0
82	209.5
83	159.5
84	146.5
85	103.0
86	56.0
87	20.0
88	4.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	3.0
95	18.0
96	30.0
97	30.0
98	15.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	18258.0
25	21694.0
26	25997.0
27	28751.0
28	31784.0
29	25753.0
30	22678.0
31	20517.0
32	18121.0
33	13815.0
34	15429.0
35	13779.0
36	12349.0
37	11713.0
38	14746.0
39	16779.0
40	19376.0
41	20334.0
42	25827.0
43	42368.0
44	101531.0
45	211369.0
46	227045.0
47	263764.0
48	317404.0
49	375239.0
50	361446.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.877192468474291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.26642173278216	7.534242007899822
2	11.503139308902337	2.5024372050920616
3	4.186440883357168	1.3661016973849631
4	2.1611561350928428	0.9402924494331549
5	1.315368407221601	0.7153757666149911
6	0.9527347522450913	0.6217847562944415
7	0.69280453387876	0.5275037780621623
8	0.5471683185635284	0.47613240909335625
9	0.4629133671314539	0.4531678011466493
>10	5.010153899594892	12.985688384204266
>50	2.159103263178009	16.921464720848512
>100	1.612217936686546	29.368933324653838
>500	0.07043882979824528	5.357288385854319
>1k	0.05337600767320451	9.94416467596273
>5k	0.003500066076931443	2.5878689089296723
>10k+	0.003062557817315012	7.697553728525053
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	56520	2.481269749844811	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	36887	1.6193665474615277	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	21265	0.9335492078989722	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	12760	0.5601734254780572	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	12655	0.5555638479173051	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	11417	0.5012147334391048	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10248	0.44989476992939886	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	9886	0.4340027025294728	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	9112	0.4000235307959291	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6818	0.2993152362781656	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	6740	0.29589097866160696	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5747	0.25229754515849484	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	5535	0.24299058855964312	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	5456	0.23952243020441064	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	5086	0.22327915689509392	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC	4604	0.20211900085430837	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	4556	0.20001176539796456	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3959	0.17380302440968873	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT	3890	0.17077387344119452	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3542	0.15549641638270206	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3486	0.15303797501696761	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG	3302	0.14496023910098313	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	3136	0.13767271648112753	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	3073	0.1349069699446763	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	3060	0.1343362603419165	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	3039	0.1334143448297661	No Hit
CCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGC	2974	0.13056079681596722	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAA	2794	0.12265866385467801	No Hit
ATGTAAGCCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAG	2744	0.12046362692098658	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	2650	0.11633695748564665	No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTT	2573	0.11295660060776183	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG	2525	0.11084936515141804	No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCTT	2481	0.10891773264976956	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	2461	0.10803971787629299	No Hit
CCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGCC	2433	0.10681049719342578	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	2406	0.1056251772492324	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	2338	0.10263992701941202	No Hit
CTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAA	2305	0.10119120264317567	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.12845356135962344	0.0	0.0	0.0	0.0
2	0.210943049327748	0.0	0.0	0.0	0.0
3	0.26586287340870796	0.0	0.0	0.0	0.0
4	0.3086660936156912	0.0	0.0	0.0	0.0
5	0.3493620783663306	0.0	0.0	0.0	0.0
6	0.4070915497224156	0.0	0.0	0.0	0.0
7	0.4493679610653129	0.0	0.0	0.0	0.0
8	0.49572714110487626	0.0	0.0	0.0	0.0
9	0.5401985893814649	0.0	0.0	0.0	0.0
10	0.5860309605569424	0.0	0.0	0.0	0.0
11	0.6329169494605916	0.0	0.0	0.0	0.0
12	0.6832710967194734	0.0	0.0	0.0	0.0
13	0.732132618863445	0.0	0.0	0.0	0.0
14	0.7744090302063422	0.0	0.0	0.0	0.0
15	0.8154123201276985	0.0	0.0	0.0	0.0
16	0.8502256058960448	0.0	0.0	0.0	0.0
17	0.8849510901870434	0.0	0.0	0.0	0.0
18	0.917920544931089	0.0	0.0	0.0	0.0
19	0.9486071612640954	0.0	0.0	0.0	0.0
20	0.97617682515126	0.0	0.0	0.0	0.0
21	1.0044489008572057	0.0	0.0	0.0	0.0
22	1.027760193093009	0.0	0.0	0.0	0.0
23	1.05471524663874	0.0	0.0	0.0	0.0
24	1.0778509359198478	0.0	0.0	0.0	0.0
25	1.0999769082114577	0.0	0.0	0.0	0.0
26	1.13325366812622	0.0	0.0	0.0	0.0
27	1.1554235411565035	0.0	0.0	0.0	0.0
28	1.1800518555525215	0.0	0.0	0.0	0.0
29	1.2113969829656355	0.0	0.0	0.0	0.0
30	1.2388788453754522	0.0	0.0	0.0	0.0
31	1.265087586363728	0.0	0.0	0.0	0.0
32	1.291603632522721	0.0	0.0	0.0	0.0
33	1.32106102817286	0.0	0.0	0.0	0.0
34	1.3463917543876593	0.0	0.0	0.0	0.0
35	1.367244605257728	0.0	0.0	0.0	0.0
36	1.3884925627758613	0.0	0.0	0.0	0.0
37	1.4068869722801955	0.0	0.0	0.0	0.0
38	1.4197937894503012	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAC	25	1.5788828E-9	245.85957	44
AACGTAT	15	1.4974377E-5	245.85956	44
ACGAACA	170	0.0	173.54794	44
CTATCAT	1700	0.0	154.7469	44
TTATGCA	25	1.1479436E-4	147.51575	44
GAACGTA	15	2.473772E-4	121.66022	43
TCGTAAC	15	2.473772E-4	121.66022	43
ATATTGG	15	2.473772E-4	121.66022	43
ACTTCTA	1220	0.0	117.89168	44
GCTTCAG	720	0.0	110.97828	44
AATCGCA	300	0.0	106.539154	44
ACATTGG	465	0.0	105.746056	44
CCGGCTA	35	4.3813727E-4	105.36839	44
CGACCAG	35	4.3813727E-4	105.36839	44
GACTCAA	30	3.1355557E-7	101.383514	43
TCGCTCC	110	0.0	100.57892	44
AGATATG	75	9.669748E-9	98.343834	44
GATTCAA	80	1.513763E-8	92.19734	44
CGAGAAA	465	0.0	89.88415	44
CGCCTAC	85	2.305751E-8	86.77397	44
>>END_MODULE