FastQCFastQC Report
Tue 24 May 2016
ERR841057.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841057.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817447
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC239682.9320555338755905No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA159361.949484186742382No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT89541.0953615341422747No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT74630.9129643879052709No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA45470.5562440133733441No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC44360.5426651513798448No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA43600.533367912537449No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA39860.4876157108656585No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA34330.41996606507822526No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA33300.40736585980497814No Hit
AAAAAAAAAAAAAAAAAAAAAAAA26900.3290733221848022No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA26860.3285839938246761No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23120.28283179215288573No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC23040.2818531354326336No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA22130.27072091523976477No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG16160.19768865749094436No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16030.19609834032053453No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC15070.18435445967750816No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC14560.17811552308590037No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC14250.1743232282949231No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA13390.16380266855221196No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA13230.16184535511170756No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12980.15878705286091943No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC12830.15695207151044654No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT12640.15462776179984758No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC12050.1474101684879876No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG11840.14484119459732558No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA11460.14019257517612763No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC11410.13958091472597No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG10910.13346431022439376No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC10730.1312623326038263No Hit
TCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGC10590.12954968334338496No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA10460.12795936617297513No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA10300.12600205273247073No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT10170.12441173556206091No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT9860.12061944077108364No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA9770.1195184519607999No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA9510.11633781761998027No Hit
CCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGC9420.11523682880969653No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA8970.1097318847582779No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8810.10777457131777352No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA8690.10630658623739522No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAA8500.10398227652679624No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG8490.10385994443676472No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG8390.10263662353644945No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA8250.10092397427600812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACC400.0296.8909644
GATGGAT255.0749804E-10296.8909644
ATTGTTA351.2732926E-11254.4779844
GGTGCGT202.2190623E-5222.6682344
AATCGCA800.0222.6682344
GGTAGAG202.2190623E-5222.6682344
CTATCAT11800.0208.8300844
CGAACTG150.0027921947197.927344
GGTACAA150.0027921947197.927344
TTTAATC600.0197.927344
TAGGGAT150.0027921947197.927344
ACATTGG3000.0188.0309444
TGCATGG255.4030883E-5178.1345844
GTATCAT501.546141E-10178.1345844
TTTTATC255.4030883E-5178.1345844
ATATTCC255.4030883E-5178.1345844
TATCAGA700.0169.6519844
CATCGAA552.9831426E-10161.9405444
GGATGTC200.00660187148.4454844
CATAGAT301.11737696E-4148.4454844
Produced by FastQC (version 0.11.5)