##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841057.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	817447
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.575758428375174	39.0	37.0	39.0	32.0	39.0
2	37.37974816715946	39.0	37.0	39.0	35.0	39.0
3	37.80720340278942	39.0	37.0	39.0	35.0	39.0
4	38.48211566009784	39.0	38.0	41.0	35.0	41.0
5	38.932329557757264	40.0	38.0	41.0	35.0	41.0
6	38.904874566791484	40.0	38.0	41.0	35.0	41.0
7	38.923141194474994	40.0	38.0	41.0	35.0	41.0
8	38.913957724476326	40.0	38.0	41.0	35.0	41.0
9	38.91239554368662	40.0	38.0	41.0	35.0	41.0
10	38.82031740284079	40.0	38.0	41.0	35.0	41.0
11	38.777245497261596	40.0	38.0	41.0	35.0	41.0
12	38.73213920902517	40.0	38.0	41.0	35.0	41.0
13	38.57086759141571	40.0	38.0	41.0	35.0	41.0
14	38.53725195639595	40.0	38.0	41.0	35.0	41.0
15	38.43399266252124	40.0	37.0	41.0	35.0	41.0
16	38.3907702884713	40.0	37.0	41.0	35.0	41.0
17	38.37575035445723	40.0	37.0	41.0	35.0	41.0
18	38.31533542847426	40.0	37.0	41.0	34.0	41.0
19	38.31520820310063	40.0	37.0	41.0	34.0	41.0
20	38.18563772330194	40.0	36.0	41.0	34.0	41.0
21	38.11539341388494	40.0	36.0	41.0	34.0	41.0
22	38.01571478028545	40.0	36.0	41.0	34.0	41.0
23	37.87055307561224	39.0	36.0	41.0	33.0	41.0
24	37.653285167111754	39.0	35.0	41.0	33.0	41.0
25	37.5926291575757	39.0	35.0	41.0	33.0	41.0
26	37.44942432825824	39.0	35.0	41.0	33.0	41.0
27	37.332620239816634	39.0	35.0	41.0	33.0	41.0
28	37.12744248749282	38.0	35.0	40.0	32.0	41.0
29	37.139855175166794	38.0	35.0	40.0	32.0	41.0
30	37.077725436530386	38.0	35.0	40.0	32.0	41.0
31	37.070090213282185	38.0	35.0	40.0	32.0	41.0
32	36.99262490715657	38.0	35.0	40.0	32.0	41.0
33	36.85346523119602	38.0	35.0	40.0	32.0	41.0
34	36.76276800672302	38.0	35.0	40.0	31.0	41.0
35	36.61492728487124	38.0	35.0	40.0	31.0	41.0
36	36.57694733871548	38.0	35.0	40.0	31.0	41.0
37	36.53250690777013	37.0	35.0	40.0	31.0	41.0
38	36.25691493013955	37.0	34.0	40.0	31.0	41.0
39	36.24167891030879	37.0	34.0	40.0	31.0	41.0
40	36.00574602239497	36.0	34.0	40.0	31.0	41.0
41	35.89590661608419	36.0	34.0	40.0	30.0	41.0
42	35.76097440480083	36.0	34.0	40.0	30.0	41.0
43	35.58088806417809	36.0	34.0	40.0	30.0	41.0
44	35.36714289596121	36.0	33.0	39.0	29.0	41.0
45	35.13228794955854	35.0	33.0	39.0	29.0	41.0
46	34.765647692905226	35.0	32.0	39.0	28.0	41.0
47	34.963401798916514	35.0	33.0	39.0	29.0	40.0
48	35.85021968074537	36.0	34.0	39.0	31.0	41.0
49	36.37038284225296	37.0	35.0	39.0	32.0	41.0
50	36.834755690211615	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	8.0
21	40.0
22	75.0
23	141.0
24	250.0
25	416.0
26	705.0
27	1224.0
28	2176.0
29	3965.0
30	7280.0
31	13520.0
32	24267.0
33	43251.0
34	63044.0
35	56840.0
36	92869.0
37	133759.0
38	127319.0
39	111914.0
40	134379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.17880914603638	19.454105281443322	41.367085572520295
2	19.716385282470913	23.231108561166657	36.533255367014625	20.51925078934781
3	20.908511499828123	29.68106800807881	23.809127686565613	25.601292805527454
4	17.6697694162435	32.352189193917155	30.478917899264417	19.499123490574924
5	16.19701338435397	31.028433647686025	29.757770228528578	23.016782739431424
6	18.57796285263754	30.773738236240394	23.49583520399488	27.15246370712719
7	21.35502362844319	27.19650325953854	20.5832304724343	30.865242639583972
8	20.957444335840734	27.502700480887448	30.15253588306031	21.387319300211512
9	22.140640310625642	33.306991156613215	22.417477830366984	22.13489070239416
10	28.632192668148516	25.207505807715975	23.702087107788028	22.458214416347484
11	22.579934845928847	25.079791105723064	22.194711094419578	30.14556295392851
12	20.74850112606689	27.5890669364497	23.760317182643035	27.902114754840373
13	21.478579039375028	26.811768836389394	23.30609813235598	28.403553991879594
14	18.276536582799864	28.41896783522357	22.07054402303758	31.233951558938987
15	19.790518529030017	37.036651917494346	22.007787660851406	21.16504189262423
16	19.051510373149576	39.4885539979962	18.7745505213182	22.685385107536025
17	18.996827928905482	39.9192852869972	18.69087537173664	22.39301141236068
18	20.307249277323177	30.2029367041533	18.83461557752368	30.655198440999843
19	21.169201183685303	28.206966323198934	18.652585427556772	31.971247065558988
20	20.127176440796774	26.6872347687373	24.622636085275253	28.562952705190675
21	22.211470590753894	37.20791684353848	18.994014290834755	21.586598274872866
22	24.835249257750043	28.20855664036935	18.97431882433968	27.98187527754093
23	24.23276371434478	27.251675032142757	26.518905812853923	21.99665544065854
24	21.580970998731416	28.65372311599406	22.718781768114628	27.046524117159887
25	23.06066185544619	27.422599243763017	20.333371178090847	29.183367722699945
26	24.081301238900966	36.507728631671114	20.253467687921184	19.15750244150674
27	25.0201110051714	28.173971244735462	18.16317381338755	28.642743936705585
28	23.341517674075288	26.267120478963342	21.527592676125646	28.863769170835724
29	22.280113436150558	28.486724823714972	27.688224823064832	21.54493691706964
30	25.015066384199375	27.382396673552904	28.932589444320172	18.66994749792755
31	23.510789146738247	32.17216876348477	21.665952799644575	22.651089290132408
32	19.907781119171347	28.88817554090954	23.272171952082715	27.931871387836406
33	22.758205914543623	24.538908334326674	31.266864251579413	21.436021499550286
34	21.918843553377606	33.32328336543866	24.033838833075947	20.724034248107788
35	19.216254325663726	26.105706990052692	23.020227202137338	31.657811482146244
36	19.797505547224727	27.844301437021706	24.505176487513904	27.85301652823966
37	19.773213450096932	38.35790048354391	19.934348329879448	21.93453773647971
38	27.58251043597795	27.18300760750914	19.96561276951728	25.26886918699563
39	19.732516996433468	35.069319479317066	24.455387273104492	20.742776251144974
40	19.06817104875581	26.610572510448282	23.86876073465984	30.452495706136062
41	19.06521170146369	36.14499414696721	21.370903955614743	23.41889019595436
42	19.107110423341542	35.19481557362161	21.229993533997373	24.46808046903947
43	20.99376996169418	24.648666813070847	22.73308110545965	31.624482119775323
44	20.65483540803048	27.398532062449238	21.08356254132537	30.86306998819491
45	20.448970250240958	26.374745745774785	32.971494515200455	20.204789488783803
46	20.085631347110677	28.336023885476358	29.921639586274683	21.65670518113828
47	18.04103630333202	36.77189402793292	24.24447873821869	20.942590930516367
48	28.069458856776986	23.35653412227055	22.617733424591	25.956273596361466
49	15.915814792543594	27.36941270795751	21.982762076568452	34.73201042293045
50	17.455000517505812	31.041880333838296	29.096058487565745	22.40706066109015
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	45013.0
1	41910.5
2	25801.0
3	12367.5
4	11211.5
5	8304.0
6	4359.0
7	2628.0
8	2507.5
9	2210.5
10	1948.0
11	1810.0
12	1745.0
13	1638.0
14	1669.0
15	1723.5
16	1643.0
17	1632.0
18	1692.5
19	1782.0
20	1856.5
21	2008.5
22	2359.5
23	3121.0
24	3917.5
25	4349.0
26	5264.0
27	6555.5
28	7256.0
29	7786.0
30	9078.5
31	12858.5
32	18779.0
33	21247.0
34	22881.0
35	26756.5
36	27095.5
37	24967.0
38	24556.5
39	26590.0
40	28777.5
41	30802.5
42	33803.0
43	36179.5
44	39594.5
45	43665.5
46	48006.0
47	49313.5
48	56714.5
49	69876.0
50	86707.0
51	102008.5
52	93663.5
53	77482.5
54	70471.5
55	67136.5
56	61492.0
57	55820.5
58	54310.0
59	49581.0
60	41316.5
61	35841.0
62	31495.0
63	26062.5
64	22031.5
65	19633.0
66	15107.0
67	10696.0
68	7696.0
69	5056.0
70	3804.5
71	2944.0
72	2076.5
73	1431.0
74	891.5
75	494.0
76	302.0
77	185.5
78	118.5
79	75.0
80	37.0
81	23.0
82	25.5
83	37.0
84	41.5
85	39.0
86	28.5
87	12.0
88	4.0
89	4.0
90	2.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	7119.0
25	8569.0
26	9904.0
27	11503.0
28	11285.0
29	9097.0
30	8196.0
31	7241.0
32	6285.0
33	5244.0
34	5370.0
35	4750.0
36	4852.0
37	4395.0
38	5106.0
39	5784.0
40	7381.0
41	7151.0
42	9728.0
43	16061.0
44	41984.0
45	77183.0
46	78646.0
47	102269.0
48	112894.0
49	143171.0
50	106279.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.854346520324865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.30648173947144	7.9787435760361225
2	11.579620857971374	2.7453767644874834
3	4.542687016913821	1.6155175809563187
4	2.3322291363528476	1.1058820938849858
5	1.4798303458097273	0.8771210855260342
6	1.1062609000753332	0.7868400030827687
7	0.8606544689018916	0.7141747416040428
8	0.6831573841883121	0.6478707488069563
9	0.5985366810109078	0.6385735099645604
>10	5.860499674932665	16.094866089177646
>50	2.044312353590704	17.21175807116547
>100	1.4963417025272696	29.274436140814018
>500	0.07326914543409388	5.907416627622341
>1k	0.03199075364023817	7.511557324205728
>5k	0.0020639195896927855	2.0083259220475456
>10k+	0.0020639195896927855	4.881539720617973
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	23968	2.9320555338755905	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	15936	1.949484186742382	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	8954	1.0953615341422747	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	7463	0.9129643879052709	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	4547	0.5562440133733441	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	4436	0.5426651513798448	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	4360	0.533367912537449	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	3986	0.4876157108656585	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3433	0.41996606507822526	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3330	0.40736585980497814	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	2690	0.3290733221848022	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	2686	0.3285839938246761	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2312	0.28283179215288573	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	2304	0.2818531354326336	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	2213	0.27072091523976477	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	1616	0.19768865749094436	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1603	0.19609834032053453	No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC	1507	0.18435445967750816	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1456	0.17811552308590037	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC	1425	0.1743232282949231	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	1339	0.16380266855221196	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	1323	0.16184535511170756	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1298	0.15878705286091943	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	1283	0.15695207151044654	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT	1264	0.15462776179984758	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	1205	0.1474101684879876	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	1184	0.14484119459732558	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	1146	0.14019257517612763	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	1141	0.13958091472597	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1091	0.13346431022439376	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	1073	0.1312623326038263	No Hit
TCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGC	1059	0.12954968334338496	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA	1046	0.12795936617297513	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	1030	0.12600205273247073	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT	1017	0.12441173556206091	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	986	0.12061944077108364	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	977	0.1195184519607999	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	951	0.11633781761998027	No Hit
CCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGC	942	0.11523682880969653	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	897	0.1097318847582779	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	881	0.10777457131777352	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	869	0.10630658623739522	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAA	850	0.10398227652679624	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	849	0.10385994443676472	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG	839	0.10263662353644945	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	825	0.10092397427600812	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.12318841466174565	0.0	0.0	0.0	0.0
2	0.22851634417888866	0.0	0.0	0.0	0.0
3	0.301303937747646	0.0	0.0	0.0	0.0
4	0.35231641929079194	0.0	0.0	0.0	0.0
5	0.39501031871179415	0.0	0.0	0.0	0.0
6	0.457889012987998	0.0	0.0	0.0	0.0
7	0.5053538639202297	0.0	0.0	0.0	0.0
8	0.5590576514440692	0.0	0.0	0.0	0.0
9	0.6015068866850083	0.0	0.0	0.0	0.0
10	0.6439561219259475	0.0	0.0	0.0	0.0
11	0.6878733422472649	0.0	0.0	0.0	0.0
12	0.7365615140798119	0.0	0.0	0.0	0.0
13	0.7783990888705934	0.0	0.0	0.0	0.0
14	0.8180346860408075	0.0	0.0	0.0	0.0
15	0.859382932471463	0.0	0.0	0.0	0.0
16	0.8973058803812357	0.0	0.0	0.0	0.0
17	0.9340055073906932	0.0	0.0	0.0	0.0
18	0.9800023732425466	0.0	0.0	0.0	0.0
19	1.0064261046893561	0.0	0.0	0.0	0.0
20	1.0289352092551567	0.0	0.0	0.0	0.0
21	1.0562152653321866	0.0	0.0	0.0	0.0
22	1.0811710116986177	0.0	0.0	0.0	0.0
23	1.1085733998656793	0.0	0.0	0.0	0.0
24	1.131816496971669	0.0	0.0	0.0	0.0
25	1.1513896313767131	0.0	0.0	0.0	0.0
26	1.1767123740132388	0.0	0.0	0.0	0.0
27	1.2006894636594176	0.0	0.0	0.0	0.0
28	1.2273578592862902	0.0	0.0	0.0	0.0
29	1.253659258643068	0.0	0.0	0.0	0.0
30	1.2791043333696253	0.0	0.0	0.0	0.0
31	1.305405732726403	0.0	0.0	0.0	0.0
32	1.3286488298323929	0.0	0.0	0.0	0.0
33	1.3550725612792023	0.0	0.0	0.0	0.0
34	1.3818632889961062	0.0	0.0	0.0	0.0
35	1.4029044084815285	0.0	0.0	0.0	0.0
36	1.4219882145264464	0.0	0.0	0.0	0.0
37	1.4427846698318056	0.0	0.0	0.0	0.0
38	1.4529382333044223	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTACC	40	0.0	296.89096	44
GATGGAT	25	5.0749804E-10	296.89096	44
ATTGTTA	35	1.2732926E-11	254.47798	44
GGTGCGT	20	2.2190623E-5	222.66823	44
AATCGCA	80	0.0	222.66823	44
GGTAGAG	20	2.2190623E-5	222.66823	44
CTATCAT	1180	0.0	208.83008	44
CGAACTG	15	0.0027921947	197.9273	44
GGTACAA	15	0.0027921947	197.9273	44
TTTAATC	60	0.0	197.9273	44
TAGGGAT	15	0.0027921947	197.9273	44
ACATTGG	300	0.0	188.03094	44
TGCATGG	25	5.4030883E-5	178.13458	44
GTATCAT	50	1.546141E-10	178.13458	44
TTTTATC	25	5.4030883E-5	178.13458	44
ATATTCC	25	5.4030883E-5	178.13458	44
TATCAGA	70	0.0	169.65198	44
CATCGAA	55	2.9831426E-10	161.94054	44
GGATGTC	20	0.00660187	148.44548	44
CATAGAT	30	1.11737696E-4	148.44548	44
>>END_MODULE