FastQCFastQC Report
Tue 24 May 2016
ERR841056.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841056.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615873
Sequences flagged as poor quality0
Sequence length24-50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC75501.225902093451085No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA47760.7754845560691895No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT33480.5436185707118188No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA32590.5291675394115345No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA31710.5148788792494556No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA29930.485976816648887No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT28480.46243300160909795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA25240.4098247528305348No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA24500.3978092886033322No Hit
AAAAAAAAAAAAAAAAAAAAAAAA19530.3171108329152276No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC19270.31288918332188614No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16660.2705103162502659No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16430.26677578007154074No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA12410.20150258251295317No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA11300.18347938617214912No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA10800.17536082926187704No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9840.15977319999415465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9600.15587629267722403No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9540.1549020658479914No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8310.13493041584872204No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA8250.1339561890194894No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC7840.12729897235306628No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG7270.11804381747535612No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC7070.11479639471124728No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7070.11479639471124728No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA6910.11219845649996021No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC6900.11203608536175477No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6490.10537886869533167No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA6390.10375515731327724No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG6320.10261855934583916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA251.5306796E-8168.055344
GCGAGCC251.5306796E-8168.055344
AGATATC100.004556988168.0552844
TACCACG302.3101165E-10168.0552844
CATGCGG156.82004E-5168.0552844
GATCATA100.004556988168.0552844
ATCGTGC156.82004E-5168.0552844
CCGTATA353.6379788E-12168.0552844
AGTCGCT356.7666406E-10144.047444
CGAAGGA356.7666406E-10144.047444
TACGCCG202.1452544E-4126.0414644
AATTAGG207.188002E-6113.5103443
ATAACGT358.0035534E-11113.5103443
GTCATGC153.2566432E-4113.5103343
CCCTACG153.2566432E-4113.5103343
GGGTATG153.2566432E-4113.5103343
GTATGAC251.5951264E-7113.5103343
GCCCCCT800.0105.0345544
GGTACCA402.529141E-7105.0345544
GTGTCTA402.529141E-7105.0345544
Produced by FastQC (version 0.11.5)