##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841056.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	615873
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.68346883204816	39.0	37.0	39.0	32.0	39.0
2	37.30306735317184	39.0	37.0	39.0	34.0	39.0
3	37.54953050385388	39.0	37.0	39.0	35.0	39.0
4	37.94002822010382	39.0	37.0	39.0	35.0	41.0
5	38.83894406801402	40.0	38.0	41.0	35.0	41.0
6	38.828102547116046	40.0	38.0	41.0	35.0	41.0
7	38.89554339936968	40.0	38.0	41.0	35.0	41.0
8	38.83740478962383	40.0	38.0	41.0	35.0	41.0
9	38.85358344983462	40.0	38.0	41.0	35.0	41.0
10	38.79868414429598	40.0	38.0	41.0	35.0	41.0
11	38.73199669412362	40.0	38.0	41.0	35.0	41.0
12	38.69101097141781	40.0	38.0	41.0	35.0	41.0
13	38.573438030243246	40.0	38.0	41.0	35.0	41.0
14	38.58002705103163	40.0	38.0	41.0	35.0	41.0
15	38.49284186837221	40.0	37.0	41.0	35.0	41.0
16	38.37839619531949	40.0	37.0	41.0	35.0	41.0
17	38.29062485285115	40.0	36.0	41.0	34.0	41.0
18	38.205941809431486	40.0	36.0	41.0	34.0	41.0
19	38.16662526202642	40.0	36.0	41.0	34.0	41.0
20	38.083419795964424	40.0	36.0	41.0	34.0	41.0
21	37.98437177794773	40.0	35.0	41.0	34.0	41.0
22	37.87580231638666	39.0	35.0	41.0	33.0	41.0
23	37.83205953175411	39.0	35.0	41.0	33.0	41.0
24	37.65861305821168	39.0	35.0	41.0	33.0	41.0
25	37.587960597007104	39.0	35.0	41.0	33.0	41.0
26	37.4864453818644	39.0	35.0	41.0	33.0	41.0
27	37.40780030261237	39.0	35.0	41.0	33.0	41.0
28	37.33443059774355	39.0	35.0	41.0	33.0	41.0
29	37.3035948840252	38.0	35.0	41.0	33.0	41.0
30	37.29641417098225	38.0	35.0	40.0	33.0	41.0
31	37.24734785819923	38.0	35.0	40.0	33.0	41.0
32	37.25036262904287	38.0	35.0	40.0	33.0	41.0
33	37.12907451562701	38.0	35.0	40.0	33.0	41.0
34	37.0717625679665	38.0	35.0	40.0	32.0	41.0
35	36.95846909365745	38.0	35.0	40.0	32.0	41.0
36	36.87162210150404	38.0	35.0	40.0	32.0	41.0
37	36.824995280693365	38.0	35.0	40.0	32.0	41.0
38	36.666779109270244	38.0	35.0	40.0	31.0	41.0
39	36.58202761812679	38.0	35.0	40.0	31.0	41.0
40	36.378529593383355	37.0	34.0	40.0	31.0	41.0
41	36.30798517220564	37.0	34.0	40.0	31.0	41.0
42	36.16272593385995	37.0	34.0	40.0	31.0	41.0
43	36.04882463864075	37.0	34.0	40.0	30.0	41.0
44	35.84953840574568	36.0	34.0	40.0	30.0	41.0
45	35.63561443386261	36.0	34.0	40.0	29.0	41.0
46	35.249862944471644	36.0	33.0	40.0	28.0	41.0
47	35.155101974818905	36.0	33.0	39.0	28.0	41.0
48	36.019464576705204	37.0	34.0	39.0	31.0	41.0
49	36.99059866712908	38.0	35.0	40.0	33.0	41.0
50	36.713358382826286	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	6.0
21	18.0
22	32.0
23	71.0
24	162.0
25	280.0
26	496.0
27	849.0
28	1587.0
29	3032.0
30	5481.0
31	10160.0
32	18255.0
33	31873.0
34	49856.0
35	38846.0
36	62398.0
37	99342.0
38	91238.0
39	82829.0
40	119059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.237555470040085	17.69520664162904	30.067237888330872
2	23.36585627231588	29.31448529161045	30.828596155376193	16.491062280697484
3	22.917874302007068	28.658181150984053	24.423054753171513	24.000889793837366
4	18.897240177763923	30.25834871799868	29.398106427786246	21.446304676451152
5	15.587466896584198	33.87678953290695	26.654034192114285	23.88170937839457
6	17.37598498391714	34.63197769670045	24.812258371449957	23.179778947932448
7	24.316376915370537	34.08998283737069	20.055433506583338	21.53820674067543
8	23.1396732767957	31.00736677854038	24.44270166089437	21.410258283769544
9	20.922170642323987	35.59207823690923	25.045585697051177	18.440165423715605
10	24.026869175950235	27.556005864845513	24.653134656008625	23.763990303195627
11	21.69749282725497	31.36929204560031	24.92932796209608	22.00388716504864
12	20.09099278585033	29.609676020867937	26.936559972591752	23.36277122068998
13	19.722735044400387	29.540343544854213	26.042869227908998	24.694052182836394
14	16.609917953863864	32.41431269109054	27.070353790473035	23.905415564572564
15	18.117858714377803	35.713369477148696	23.965005772293964	22.203766036179537
16	16.953820024582992	41.33985415824367	23.31000060077321	18.396325216400133
17	20.670008264690935	40.228910830642036	19.2338355472638	19.86724535740323
18	20.043093300079722	31.745668343960524	22.19012686057028	26.02111149538947
19	20.103657734630353	31.230951835849275	24.094740311720113	24.57065011780026
20	19.197789154582196	31.057539460245863	24.66092847064249	25.083742914529456
21	19.40318864441208	36.67135919256081	22.97080729306204	20.954644869965076
22	19.501585554164578	35.26522513570168	22.27342325446967	22.959766055664073
23	20.00477371146324	32.84654466099342	25.58806766979556	21.560613957747783
24	20.64662682078935	31.730405456969212	25.51288983280644	22.110077889434997
25	19.704444793877926	32.126641245380924	23.935483659616846	24.233430301124304
26	20.246781595309297	35.94236908345593	23.693151446647878	20.11769787458689
27	20.04790246776375	34.30499578217265	23.203741145910044	22.443360604153558
28	19.387509492297678	31.79614612714255	22.279948199175525	26.536396181384248
29	20.864132030473325	33.63707072840314	24.40098143750719	21.097815803616346
30	21.208178212627274	32.25543624604128	26.9768443801281	19.55954116120335
31	19.632954451756195	33.37970670741442	23.868837618923145	23.118501221906243
32	21.341338295749633	31.670959251109114	23.2592037020003	23.728498751140954
33	19.636202687074345	30.08663030597239	28.215351447050928	22.06181555990234
34	19.920738671052916	34.26463299885795	25.334714810052937	20.4799135200362
35	19.803167118096113	30.997291423322686	23.983379681697038	25.21616177688416
36	18.30598356282131	31.375416347216735	24.90270814629198	25.415891943669976
37	18.22385458304239	37.53177513740971	22.003881744255732	22.240488535292165
38	22.41607818631924	30.97904327934245	23.563730071391838	23.041148462946477
39	19.21688636334112	34.72846752986458	25.927300598126745	20.12734550866755
40	17.98562901143317	32.85847944462117	26.377500421025058	22.778391122920606
41	20.111567988161987	35.09717730719231	22.239231588727396	22.552023115918306
42	17.150316546487385	37.151846680565384	24.174014498020387	21.52382227492684
43	20.088205342657233	29.82602870739291	23.459407343733666	26.626358606216193
44	20.6412651860962	30.129569625442475	24.217339545737644	25.011825642723686
45	19.988162226816666	28.462856931468334	30.223931209384723	21.325049632330273
46	19.476098192671873	32.026323685676964	27.40907521923244	21.08850290241872
47	18.966939030700242	33.63309546395309	25.36192437047258	22.038041134874096
48	22.20221929075813	30.76030107860635	23.32986730813999	23.70761232249554
49	19.51174932278534	30.34905846073092	23.733444562130344	26.40574765435339
50	19.708967987825407	33.46868140007811	25.060940298708466	21.761410313388012
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	38021.0
1	33773.0
2	19980.0
3	9837.5
4	8514.5
5	6186.5
6	3435.0
7	2225.5
8	1957.0
9	1552.5
10	1285.5
11	1340.5
12	1537.5
13	1614.0
14	1839.0
15	2375.5
16	2859.5
17	3160.5
18	3397.0
19	3908.5
20	4566.0
21	5169.5
22	5921.0
23	7097.0
24	8422.5
25	9770.0
26	10833.0
27	12071.5
28	13503.0
29	14114.0
30	15286.5
31	17854.5
32	20832.5
33	23304.0
34	27019.5
35	30338.0
36	29444.5
37	26953.0
38	26070.5
39	28173.0
40	31048.5
41	34318.5
42	37530.5
43	37569.5
44	37347.5
45	39695.5
46	42123.0
47	40078.5
48	40764.0
49	46134.0
50	50751.0
51	53695.5
52	49027.5
53	39657.0
54	35422.5
55	33597.5
56	30328.0
57	28704.5
58	27448.0
59	23680.5
60	19921.5
61	18342.0
62	16596.0
63	13513.5
64	10107.5
65	7917.5
66	6089.0
67	4129.0
68	2689.5
69	1868.5
70	1469.0
71	1237.0
72	1077.5
73	907.5
74	759.5
75	585.5
76	444.5
77	283.5
78	144.0
79	123.0
80	121.5
81	117.0
82	95.5
83	65.0
84	32.5
85	20.5
86	10.5
87	4.5
88	0.5
89	0.0
90	0.0
91	0.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	5361.0
25	6253.0
26	6795.0
27	7512.0
28	7541.0
29	6784.0
30	5616.0
31	4695.0
32	5004.0
33	3420.0
34	3834.0
35	3937.0
36	3490.0
37	3132.0
38	3650.0
39	4439.0
40	5943.0
41	5637.0
42	6308.0
43	9150.0
44	20794.0
45	43327.0
46	72179.0
47	48897.0
48	108801.0
49	64868.0
50	148506.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.994044226650624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.58969777570226	11.450087923971338
2	10.6473914500066	3.4058969949973457
3	3.9369359308853538	1.889025821882109
4	1.9877567180695004	1.2716907544250196
5	1.2456473406901312	0.9961469328903849
6	0.9349969036476046	0.8972629097232709
7	0.691349502045623	0.7740232158253406
8	0.5573434311645331	0.7131340389983
9	0.41420058272336885	0.5962268194903819
>10	4.951118240053602	19.040451521661122
>50	1.8913129549353827	21.51255210083897
>100	1.1126564673157162	27.826191438819365
>500	0.023349542653523243	2.5545851173862144
>1k	0.015227962600123855	5.84682231563975
>5k	0.0010151975066749236	1.225902093451085
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	7550	1.225902093451085	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	4776	0.7754845560691895	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	3348	0.5436185707118188	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	3259	0.5291675394115345	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	3171	0.5148788792494556	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	2993	0.485976816648887	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	2848	0.46243300160909795	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2524	0.4098247528305348	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	2450	0.3978092886033322	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	1953	0.3171108329152276	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	1927	0.31288918332188614	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1666	0.2705103162502659	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1643	0.26677578007154074	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	1241	0.20150258251295317	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	1130	0.18347938617214912	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	1080	0.17536082926187704	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	984	0.15977319999415465	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	960	0.15587629267722403	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	954	0.1549020658479914	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	831	0.13493041584872204	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	825	0.1339561890194894	No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC	784	0.12729897235306628	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	727	0.11804381747535612	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	707	0.11479639471124728	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	707	0.11479639471124728	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	691	0.11219845649996021	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	690	0.11203608536175477	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	649	0.10537886869533167	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	639	0.10375515731327724	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	632	0.10261855934583916	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.10456701300430446	0.0	0.0	0.0	0.0
2	0.19338402560268106	0.0	0.0	0.0	0.0
3	0.26417784186025367	0.0	0.0	0.0	0.0
4	0.32003351340292563	0.0	0.0	0.0	0.0
5	0.37280413331969414	0.0	0.0	0.0	0.0
6	0.4387268154311035	0.0	0.0	0.0	0.0
7	0.4890618682747904	0.0	0.0	0.0	0.0
8	0.5436185707118188	0.0	0.0	0.0	0.0
9	0.5939536235555057	0.0	0.0	0.0	0.0
10	0.6394175422530295	0.0	0.0	0.0	0.0
11	0.6946237292428796	0.0	0.0	0.0	0.0
12	0.7443092975337448	0.0	0.0	0.0	0.0
13	0.799515484523595	0.0	0.0	0.0	0.0
14	0.8573196097247322	0.0	0.0	0.0	0.0
15	0.9008350747637905	0.0	0.0	0.0	0.0
16	0.944350539802849	0.0	0.0	0.0	0.0
17	0.990301571914989	0.0	0.0	0.0	0.0
18	1.0349536349214854	0.0	0.0	0.0	0.0
19	1.0688892028064227	0.0	0.0	0.0	0.0
20	1.1091572450813723	0.0	0.0	0.0	0.0
21	1.1455283800393912	0.0	0.0	0.0	0.0
22	1.1747551849163707	0.0	0.0	0.0	0.0
23	1.2007345670292415	0.0	0.0	0.0	0.0
24	1.22963662962981	0.0	0.0	0.0	0.0
25	1.2606495170270493	0.0	0.0	0.0	0.0
26	1.2989691056435337	0.0	0.0	0.0	0.0
27	1.33436601377232	0.0	0.0	0.0	0.0
28	1.3694381796246953	0.0	0.0	0.0	0.0
29	1.4054845723063034	0.0	0.0	0.0	0.0
30	1.4426675629553496	0.0	0.0	0.0	0.0
31	1.4795258113279848	0.0	0.0	0.0	0.0
32	1.515247461733182	0.0	0.0	0.0	0.0
33	1.5511314832765846	0.0	0.0	0.0	0.0
34	1.5889639584784525	0.0	0.0	0.0	0.0
35	1.6190026190464593	0.0	0.0	0.0	0.0
36	1.6440077743300974	0.0	0.0	0.0	0.0
37	1.6664149914024482	0.0	0.0	0.0	0.0
38	1.680703651564527	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTA	25	1.5306796E-8	168.0553	44
GCGAGCC	25	1.5306796E-8	168.0553	44
AGATATC	10	0.004556988	168.05528	44
TACCACG	30	2.3101165E-10	168.05528	44
CATGCGG	15	6.82004E-5	168.05528	44
GATCATA	10	0.004556988	168.05528	44
ATCGTGC	15	6.82004E-5	168.05528	44
CCGTATA	35	3.6379788E-12	168.05528	44
AGTCGCT	35	6.7666406E-10	144.0474	44
CGAAGGA	35	6.7666406E-10	144.0474	44
TACGCCG	20	2.1452544E-4	126.04146	44
AATTAGG	20	7.188002E-6	113.51034	43
ATAACGT	35	8.0035534E-11	113.51034	43
GTCATGC	15	3.2566432E-4	113.51033	43
CCCTACG	15	3.2566432E-4	113.51033	43
GGGTATG	15	3.2566432E-4	113.51033	43
GTATGAC	25	1.5951264E-7	113.51033	43
GCCCCCT	80	0.0	105.03455	44
GGTACCA	40	2.529141E-7	105.03455	44
GTGTCTA	40	2.529141E-7	105.03455	44
>>END_MODULE