FastQCFastQC Report
Tue 24 May 2016
ERR841055.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841055.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376381
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC431011.8137243144091795No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA284721.1981243748372No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT164100.6905458341907295No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT163060.686169431585255No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACC102200.4300657175764324No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC95700.40271320129221705No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAA74680.3142593717084929No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA74070.31169244325720497No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA69430.2921669547097035No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC68890.2898945918183995No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA60780.2557670676545554No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT55060.23169685332444587No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT54820.2306869142616441No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG47490.19984169205190583No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC46610.1961385821549659No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC46080.19390830005794527No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG45130.18991062460102145No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT44700.18810115044683492No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA40830.1718158830591559No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA39860.16773404601366532No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG37500.15780297856278097No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA37290.1569192818828294No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA36590.1539736262829908No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36380.15308992960303924No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT35670.15010219320891724No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC32830.1381512476324293No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC32760.13785668207244545No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG32680.13752003571817817No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT31200.13129207816423377No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA30860.12986133115859788No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA30760.1294405232157638No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA30500.12834642256439519No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT29010.12207638421616737No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA28280.11900448623347855No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT26820.11286069026810094No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT26580.11185075120529915No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC26190.11020960022824623No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA25510.10734810621697446No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACAC25150.10583319762277177No Hit
AAAAAAAAAAAAAAAAAAAAAAAA24630.10364499632003454No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG24630.10364499632003454No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT24440.10284546122864978No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA23890.10053101754306233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGC203.994337E-7202.9083444
CATGCGA254.980393E-9202.9083144
CGATCAG455.456968E-12157.8175844
CCGCCGT2100.0135.2722244
CGTCGCC150.008725307135.272244
CTATCAT32450.0122.8705244
ATGCGAA701.8189894E-12115.9476244
TGCCCGG805.456968E-12101.4541744
TATACAC401.2573866E-5101.4541744
ACTTCTA16300.098.9644944
CTTAACC23600.094.5759144
CCTTAAC69000.094.2060243
CCGATAA1000.091.3087444
ACATTGG7650.090.1814744
ACCAGCG452.25658E-590.1814744
ATCGGCT452.25658E-590.1814744
CCAGAAT7150.089.3931744
AATGCGA552.910383E-1183.1561843
TAATTCC5500.079.318744
ATGCGTT251.4603793E-678.2954642
Produced by FastQC (version 0.11.5)